The bcr-abl negative myeloproliferative neoplasms (MPN) include polycythemia vera (PV),essential thrombocythemia (ET) and primary myelofibrosis (PMF).Along with the detecting of JAK2 V617F gene mutation in MPN patients,a series of small molecules drugs that targeted to the mutation have been investigated in clinical trials.Ruxolitinib is the inhibitor of JAK1/2,which has been proved by USA Food and Drug Administration (FDA) and the European Medicines Agency (EMA) to treat patients with intermediate-2 or high-risk PMF or post-PV MF or post-ET MF and PV patients who are intolerant or resistant to hydroxycarbamide,attributed to a number of clinical trials,especially in COMFORT-Ⅰ and COMFORT-Ⅱ.As a result,the MPN patients have new hope for the treatment.

Objective To observe the application effect of stat lock device on fixing butterfly needles. Methods A total of 124 patients with implantable venous access port and venous chemotherapy from our hospital were selected by cluster sampling during April 2015 to July 2015. According to the odd and even-numbered day grouping method, all patients were divided into control group(64 patients) and experimental group(60 patients). Patients in experimental group use stat lock and transparent dressing fixing butterfly needles, and patients in control group use sterile gauze and transparent dressing fixing butterfly needles, statistical cases of comfort changing and drop speed slower and repeated puncture of two groups were compared. Results The comfort of patients fixed butterfly needles in control group were lower than those in experimental group(P<0.01), but the drop speed and repeated puncture in experimental group were lower than those in control group ( P<0. 05 ) . Conclusions The method of stat lock fixing butterfly needles can effectively improve the comfort of patients and cut down the drop speed and repeated puncture, which is suitable for widespread clinical application.

Objective To study the protective effect of lipoxin A4(LXA4)against sepsis-induced acute kidney in-jury(SAKI)in rats and its effect on the expression of toll-like receptor 4(TLR4),myeloid differentiation factor88(MyD88),nuclear factor kappa B(NF-κB)in the kidney.Methods Forty male specific pathogen-free C57BL/6J mice were randomly divided into SAKI group,SAKI+LXA4 group,sham procedure group,sham procedure+LXA4 group.The mice in SAKI group and SAKI+LXA4 group were given cecum ligation and puncture(CLP)to establish SAKI animal models.The mice in SAKI+LXA4 group and sham procedure+LXA4 group were given LXA4(40 ng/kg)with intraperitoneal injection 30 mins after CLP.All mice were sacrificed at the 24th hour after CLP to collect serum,urine and kidney tissues.The enzyme linked immunosorbent assay(ELISA)was used to test the serum creat-inine(Scr),blood urea nitrogen(Bun),interleukin-1 β(IL-1β),IL-6,tumor necrosis factor-α(TNF-α)and urine neutrophil gelatinase-associated lipocalin(NGAL),kidney injury molecule 1(KIM-1)levels of mice.The pathologi-cal changes were examined through hematoxylin-eosin(HE)staining and Periodic Acid-Schiff(PAS)staining.And the mRNA levels of TLR4,MyD88,NF-κB p65 were detected through real-time PCR(RT-PCR);the expression lev-els of TLR4,MyD88,NF-κB p65,phospho-NF-KB p65(p-NF-KB p65)were detected through immunohistochemistry(IHC)and Western blot assay.Results ELISA showed that the values of Scr,Bun,IL-1 β,IL-6,TNF-α,NGAL,KIM-1 in SAKI group were higher than those in SAKI+LXA4 group(P＜0.05),and there were no significant differences in Scr,Bun,IL-1 β,IL-6,TNF-α,NGAL,KIM-1 between sham procedure group and sham procedure+LXA4 group.HE and PAS staining showed that SAKI group had severer pathological changes than SAKI+LXA4 group in the kidney structure(P＜0.05),while pathological structures of the kidney were normal in sham proce-dure group and sham procedure+LXA4 group.RT-PCR showed that the mRNA levels of TLR4,MyD88,N F-κB p65 in SAKI group,SAKI+LXA4 group were higher than sham procedure group and sham procedure+LXA4 group(P＜0.05);the mRNA levels of TLR4,MyD88,NF-κB p65 in SAKI group were higher than SAKI+LXA4 group(P＜0.05);The results of IHC,Western blot assay were as follows:The expression levels of TLR4,MyD88,NF-κB p65,p-NF-κB p65 in SAKI group,SAKI+LXA4 group were higher than sham procedure group and sham procedure+LXA4 group(P＜0.05);the expression levels of TLR4,MyD88,NF-κB p65,p-NF-κB p65 in SAKI group were higher than SAKI+LXA4 group(P＜0.05).Conclusion TLR4/MyD88/NF-KB pathway plays an important role in the occurrence and development of SAKI,and LXA4 may reduce SAKI by inhibiting TLR4/MyD88/NF-κB sig-naling pathway.

Objective To explore the effect of journal club in cultivating the scientific research ability of oncology specialized nurses.Methods By cluster sampling method,a total of 45 oncology specialized nurses attending specialty training in Chinese PLA General Hospital in October 2014 and May 2015 were selected as the control group. A total of 48 oncology specialized nurses attending specialty training in October 2015 and May 2016 were selected as the experimental group. For two groups,training courses such as lecture, group discussion,and written assignment were given to them. The training lasted for a month. Furthermore, the experimental group conducted journal club for 5 times in 3 weeks,and it lasted 2 hours each time. After training,the score of a review completed by all nurses was compared between two groups,and the nurses′ opinions on journal club were investigated in the experimental group.Results The score of review in the experimental group(88.19±3.04)was higher than that(84.45±3.45)in the control group(t=-5.48,P<0.05). In the experimental group,95.83% of nurses believed that journal club training can increase innovative consciousness,93.75% of them thought that journal club training can improve the scientific research ability,and 91.67% of them thought that journal club training can raise self-learning ability.Conclusions The journal club training method can significantly improve the document retrieval ability,learning consciousness and innovation ability. It can promote comprehensive scientific research ability of oncology specialized nurses.