1.Radix Astragali decreases the risk of insulin resistance in insulin-treated diabetic rats through lowering oxidative stress
Shuhan XIONG ; Yun LI ; Fanzhu MENG ; Shuping REN
Journal of Chinese Physician 2017;19(4):552-555
Objective Fluctuation of glucose levels is more likely to cause oxidative stress which contributes to the development of insulin resistance through activating c-Jun N-terminal kinase (JNK).Such antio xidants as vitamin C or vitamin E do not appear very helpful.Radix Astragali (RA) is an herbal medicine with antioxidative ability.The study was to explore whether RA would lower the risk of insulin resistance in diabetic rats.Methods Diabetic rats received RA,insulin or both RA and insulin after diabetes were induced in male Wistar rats.Serum levels of interleukin 6 (IL-6) and tumor necrosis factor α (TNFα),advanced glycation endproducts(AGEs) levels in kidney,the expression of insulin receptor (IR),insulin receptor substrate 1 (IRS-1),p38 mitogen-activated protein kinase (MAPK),p-p38 MAPK,p-JNK,p-IRS-1 Ser307,and p-IRS-1 Tyr612 in skeletal muscles were determined.Results Compared to diabetic rats treated with insulin,the diabetic rats treated with both insulin and RA demonstrated significantly lower levels of IL-6,TNF-α and AGEs (P < 0.05),significantly lower activation of p-p38 MAPK and JNK (P <0.05),significantly higher expressions of IRS-1 (P <0.05),p-IRS-1 Tyr612,and significantly lower expression of p-IRS-1 Ser307 (P < 0.05).Conclusions RA can lower the risk of insulin resistance through fighting oxidative stress in diabetic rats.
2.NF-κB and the onset and progression of hepatocellular carcinoma
Shuhan XIONG ; Ruitong GAO ; Fanzhu MENG ; Jinghui YANG
Journal of Chinese Physician 2017;19(11):1758-1760,封3
Hepatocellular carcinoma is one of the malignant tumors with highest mortality.Nuclear factor κB (NF-κB) as the mediator of many pathways,such as metabolic pathway,angiogenesis and adherence factor,is implicated in the onset and progression of hepatocellular carcinoma.Through the literature retrieval,the activity of NF-κB and its implication in the onset,progression,invasion and metastasis have been reviewed in the article.
3. Clinical significance of exosomal miR-1231 in pancreatic cancer
Shilin CHEN ; Min MA ; Lei YAN ; Shuhan XIONG ; Zhuo LIU ; Sha LI ; Teng LIU ; Song SHANG ; Yuying ZHANG ; Hui ZENG ; Hailong XIE ; Chaohui ZUO
Chinese Journal of Oncology 2019;41(1):46-49
Objective:
To investigate the expression and clinical significance of exosomal miR-1231 in plasma of pancreatic cancer (PC) patients and pancreatic cancer cells.
Methods:
A total of 16 patients who were diagnosed with pancreatic cancer in Hunan Cancer Hospital were collected from April 2016 to August 2017. Meanwhile, 16 healthy volunteers were recruited as the healthy control group at the same period. The plasma exosomes were extracted, and the levels of miR-1231 were detected by qRT-PCR in PC and healthy control groups. Moreover, the clinicopathological significance of exosomal miR-1231 expression was analyzed. Furthermore, the expression of exosomal miR-1231 was detected in several pancreatic cancer cells (MIA PaCa-2, PANC-1, SW1990, AsPC-1 and BxPc-3) and two normal pancreatic epithelial cells (HPDE and human primary pancreatic epithelial cell).
Results:
qRT-PCR results showed that the expression level of miR-1231 in plasma exosomes of pancreatic cancer patients (1.06±0.46) was significantly lower than that in healthy controls (2.30±0.99;