Aim To evaluate the effect of Cdk7 silencing on the c ell cycle control, the phosphorylation level changes of Cdk2 and pRb in human he patoblastoma HepG2 cell culture in vitro, and to validate Cdk7 as a novel ta rget for anticancer therapeutics.Method Levels of Cdk7 and the phosphorylation levels of Cdk2 and pRb were measured by Western-blotting. DNA c ontents, cell cycle and apoptosis induced by Cdk7 silencing were analyzed by flo w cytometry and ultrastructural changes of cells were observed with transmission electron microscopy.Result The phosphorylation levels of pRb a nd Cdk2 and the levels of Cdk7 decreased in a concentration-dependent manner wh en the concentration was above 100 nmol?L -1. Indice of cells arrested in G 0/G 1 phases and apoptotic cells increased in a dosage-and time-dependent manner, the difference was significant between Cdk7 ASODN and the sense control (P

The majority of patients have experienced pain or discomfort associated with surgery or mechanical ventilation in intensive care unit (ICU), and the use of analgesic drugs can relieve patients' anxiety, improve sleep, promote the disease recovery, reduce the use of sedative drug dosage and the occurrence of delirium. Because of less adverse reactions of the non opioid analgesic drugs, in recent years gradually the doctors in ICU have paid attention to them. In this study, the related literatures have been reviewed to realize the present situation of applying non opioid analgesic drugs in ICU. It was found that nowadays the application of commonly used non opioid drugs in ICU (including ketamine, non-steroidal anti-inflammatory drugs, tramadol, lidocaine, tramadol and gabapentin) can all decrease the use of sedative drugs and opioids, reduce the incidence of adverse reactions, and gradually they will obtain more and more attention.

AIM: Remnant polyp tissues are often found after polypectomy. In this study, we assessed the outcome and safety of argon plasma coagulation (APC) in the management of colorectal sessile or semipedunculated polyp remnants after polypectomy. METHODS: Sixty-one colorectal polyps of 33 patients underwent polypectomy in Department of Digestion Medicine, Tangdu Hosptial, the Fourth Military Medical University of Chinese PLA from 2004 to 2007. Colorectal polyps type was identified by pathological examination. All polyps were removed under colonoscope with the diathermic snare, and the polyp remnants were destroyed with APC (Erbe, Germany). The power output was initially set at 40-50 W. In case of visibly insufficient coagulation, the output power was increased gradually by 5 W up until the remnants were completely ablated. Follow-up endoscopies were performed 1 and 3 months after the treatment. Pathological examination and APC treatment were performed when the recurrence of polyps was found. RESULTS: ①All 61 colorectal polyps were removed with the diathermic snare and the polyp remnants were destroyed with APC successfully only once. ②During the follow-up averaged 2.4 months, effective destruction of remnant polyp tissue was obtained in 59 polyps (96.7%) in 31 patients (93.9%). Colorectal polyp of two cases relapsed one month after treatment and identified as villous adenoma. ③Final power output was positively correlated with lesion size (r =0.97, P

Acupuncture Therapy ; Adult ; Erectile Dysfunction ; therapy ; Humans ; Male

0.05). Shufeng Xuanfei Tang could decline integral of Chinese medicine syptom markedly after treatment. Shufeng Xuanfei Tang could evidently improve the lung function.Conclusion The effect Shufeng Xuanfei Tang in treating cough variant asthina is sure.

Acupuncture Points ; Acupuncture Therapy ; Cervical Vertebrae ; physiopathology ; Female ; Humans ; Middle Aged ; Treatment Outcome ; Vertigo ; physiopathology ; therapy

Purpose To determine the effect of RNase MC2 purified from momordica charantia on cell growth of hepatocellular carcino-ma ( HCC) and its underlying mechanism. Methods MTT, colony formation and nude mice model were used to examine the activity of RNase MC2 in cell proliferation. Cell cycle analysis was done by flow cytometry. Autophagy induced by RNase MC2 treatment was observed via transmission electron microscope. Western blot was performed to detect the RNase MC2-mediated changes of proteins. Re-sults In vitro and in vivo data showed that RNase MC2 markedly inhibited HCC cell proliferation, arrested cells at G2/M phase by in-creasing expression of p53 and p21, induced autophagy via upregulating Beclin-1 and LC3-Ⅱ. Furthermore, combination of RNase MC2 and Sorafenib exerted enhanced lethal effect on HCC cells. Conclusion RNase MC2 manifests significant antitumor activities and enhances the killing effect of Sorafenib in HCC via inducing cell cycle arrest and autophagy.

Objective To evaluate the efficacy of IQ200 urine sediment quantitative analyzer(IQ200) and Mejer700 urine dry chemistry analyzer(Mejer700) in detecting urine erythrocyte(RBC)and leukocyte(WBC) .Methods The WBC and RBC in 1 000 u‐rine specimens were detected by using IQ200 ,Mejer700 and sediment microscopy respectively .Taking sediment microscopy as the golden standard ,the sensitivity ,specificity and accuracy of IQ200 and Mejer700 in detecting urine RBC and WBC were analysed .Re‐sults No statistically significant differences of positive rates of RBC and WBC detected by using the three methods were found (P>0 .05) .When IQ200 and Mejer700 were combined ,the sensitivities of detection of RBC and WBC test were improved .Conclu‐sion The sensitivities of RBC and WBC detection by using IQ200 and Mejer700 are high .Combing the two methods the sensitivity and accuracy is higher ,which could improve work efficiency .Combination of the three methods should be recommended ,in order to improve the quality of urine analysis .

The improvement of quality management for medical equipment is an important part in overall quality management for modern hospital under the new circumstance of medical reform.The principles and approaches of evidence-based medicine were applied to quality management for medical equipment.In the view of generalized quality management,the core thoughts and basic framework of evidence-based quality management for medical equipment were put forward.The foundation of evidence-based management system was discussed including organization structure,PDCA quality management cycle,quality evidence procedure and overall quality-evaluating index system.

ObjectiveTo explore the safety and therapeutic effect of 8 patients with esophageal Hiatal Hernia treated by laparoscopic hernia repair.MethodsA retrospective analysis was performed on the clinical data of 8 patients with esophageal Hiatal Hernia form Jun.2009 to Jun. 2010.Among the participants,3 conducted 360-degree fundoplication,5 conducted partial(270-degree) fundoplication.Silk sutures were used for the repair of esophageal perforation in 4 patients,and patch repair was used for the other 4 cases.ResultsEight patients were treated by laparoscopic hernia repair,and all of them were cured without postoperative complications.The mean duration of surgery was ( 120 ± 30) min,with average blood loss ( 50 ± 12 ) ml.Patients had a mean postoperative hospital stay of(4.5 ± 2.5 )days.All the patients were followed up for 1 to 2 years,and no case was found to be relapsed.ConclusionTotal laparoscopic hernia repair is minimally invasive,with short recovery course,less pain after surgery,little complication and short hospitalized time.Laparoscopic Hernia repair should be the preferred effective operation method for patients with esophageal Hiatal Hernia.