Objective To investigate the clinical applied value of magnetic mitomycin microspheres and magnetic Fe 3O 4 in treating tumors and vascular diseases by embolism.Methods The embolic therapy in 106 cases useing microspheres or drugs of Fe 3O 4 under TV-guided through catheterization using Seldinger's technique were performed.Results In 94 malignant tumors and 12 benign vascular diseases.The most of tumors became small remarkably on CT,USG or BSA one month later after embolism.Pathology showed that necrosis of cancer tissue was 100 percent.Examination of blood biochemistry showed that the functions of liver and kidney had some changes,however these functions could recover to normal or better after therapy.AFP decreased significantly after embolism in most primary hepatic cancer.Changes in density of serum Fe before and after embolism showed that embolism of Fe 3O 4 microspheres could maintain serum Fe in normal condition.DSA showed that microspheres of Fe 3O 4 were moved from the near portion to the peripheral embolic arteries after embolism one month later and the clinical symptoes improved.Conclusion Fe 3O 4 is good embolic substance and can avoid some complications by embolism.

Objective To investigate experiment research of embolizing rabbits’ kidney arteries by using microspheres of Fe 3O 4 . Methods Kidney arteries in one side of 4 big white Newzeland rabbits had been embolized by using Fe 3O 4.Results Embolism of rabbits’ kidney arteries was safe and lasting . No reperfusion and lateral circulation were established. Conclusion Embolism with microspheres of Fe 3O 4 in kidney arteries is good and safe. It is valuable in clinical treatment.

Objective To investigate pathologic changes and method of magnetic microsphere(Fe3O4) embolization for tumor.Methods 18 cases with tumors were treated by embolized using Fe3O4 under fluoroscopy through catheters by Seldinger’s technique,pathological examinations were done in 15 cases before operation,including primary hepatic carcinoma in 8,renal carcinoma in 6,renal myolipoma in 3 and breast carcinoma in 1.Results Pathological examination after embolism showed that necrosis of neoplastic tissue was 100 percent.Conclusion Fe4O4 is a good embolic substance for treating tumors and there is no damage to the other tissues.

Objective To investigate the mechanism and the clinical value of magnetic microsphere(Fe_3O_4) in embolizing tumor through artery catheter.Methods 94 patients with tumor were treated by embolized using Fe_3O_4 under fluoroscopy through catheters by Seldinger's technique.Of them,5 cases of hepatocellular carcinoma underwent magnetic field putting on outside the target area,pathological examination was done in 15 cases,25 cases underwent DSA examination one month later and the DSA images before and after operation were analysed statistically.Results Pathological examination after embolism showed that necrosis of neoplastic tissue was 100 percent.The statistical analysis of DSA images was in P

Objective To investigate the differential expression of monocyte ehemoattraetant protein-1 (MCP-1) in renal biopsy tissues of patients with IgA nephropathy, and to analyse the association between these 2 markers and their effect on various pathologic types of IgA nephropathy. Methods According to pathologic type, 88 renal biopsy tissues of patients with IgA nephropathy were divided into 4 groups: a minimal change group, a mesangial proliferative glomerulonephritis group, a focal sclerosing glomerulonephritis group, and a diffused sclerosing glomerulonephritis group. Immunohistochemical staining was used to detect the in situ expression of MCP-1 and CD68 on renal biopsy tissues. The expression levels were semi-quantified by image analysis and clinical data were collected from the patients. Results The differences in glomerular MCP-1 expressions were not statistically significant among all groups, while the tubulointerstitial MCP-1 expressions were statistically different among the 4 groups, with the average scores of 1.43 ± 0. 60, 5.98 ±0.92, 10. 60 ± 0.76 and 11.65 ±0.39 for minimal change group, mesangial proliferative glomerulonephritis group, focal sclerotic glomerulonephritis, and diffused sclerotic glomerulonephritis group, respectively. The tubular and interstitial CD68 scores were 0. 75 ± 0. 71, 5. 87 ± 0. 96, 10. 42 ± 0. 61, and 11.40 ±0.49 for the 4 groups, with significant differences in both MCP-1 and CD68 among the 4 groups. Correlation analysis indicated a positive correlation between tubulointerstitial MCP-1 and CD68 (r = 0. 688, P ＜ 0. 01) . MCP-1 in tubulointerstitial was significantly correlated with 24 h urinary protein excretion (r=0.531, P＜0.01). Conclusion MCP-1 plays a critical role in mac-rophage infiltration in the kidney. MCP-1 is associated with the severity of tubulointerstitial damage and clinical prognosis.

Objective To investigate the value of MRI diagnosis in tangential osteochondral fracture.Methods 1 7 patients with tangential osteochondral fracture were examined by MRI,and the related signs were analyzed,including the subluxation or disloca-tion of patellofemoral joint,the injury or fracture of bone and cartilage,the injury of meniscus,the injury of medial patella retinacu-lum and ligament,the effusion articular capsule.we investigated the role of X-ray film,CT,MRI,compared with arthroscopy.Re-sults MRI showed subluxation of patellofemoral joint(Ⅰtype 12 cases、Ⅱtype 3 cases、Ⅲ type 1 case)in 1 6 cases,1 case had mild move of patella.All of 1 7 cases were found fracture in bone or cartilage of the inferior medial part of patella and the anterior lateral part of lateral femoral condyle.All cases were found the injury of medial patella retinaculum(Ⅰtype 7 cases、Ⅱtype 9 cases、Ⅲ type 1 case),3 cases were found the injury of MCL,2 cases were found the injury of ACL.3 cases were found the injury or tear in anteri-or horn of meniscus.1 1 cases were found articular capsule filling with simple effusion,5 cases were found articular capsule filling with hemorrhagic effusion,1 case was found articular capsule filling with hemorrhagic effusion and fat granule.Conclusion MRI can clearly show tangential osteochondral fracture and its accompanying changes:the subluxation or dislocation of patellofemoral joint, the injury or fracture of bone and cartilage,the injury of meniscus,the injury of medial patella retinaculum and other ligament,the articular capsule effusion.

The improvement of quality management for medical equipment is an important part in overall quality management for modern hospital under the new circumstance of medical reform.The principles and approaches of evidence-based medicine were applied to quality management for medical equipment.In the view of generalized quality management,the core thoughts and basic framework of evidence-based quality management for medical equipment were put forward.The foundation of evidence-based management system was discussed including organization structure,PDCA quality management cycle,quality evidence procedure and overall quality-evaluating index system.

Objective To analyse MRI and MRCP features of primary gallbladder carcinoma.Methods MRI and MRCP data of 87 cases with gallbladder carcinoma confirmed by operation and pathology were studied retrospectively.Results Four patterns of gallbladder carcinomas were found by MRI:wall thickening (35 cases),papillary nodular(17 cases),mass-type (25 cases) and mixed (wall thickening and nodular) (10 cases).Pathology showed the tumors in combination with chronic cholecystitis and cholecystolithiasis in 69 cases(79.31%).In comparison with that of operations,the accurate diagnostic rates were 46/50(92%) with MR imaging,13/19(68.4%) with MRCP and 80/82(97.6%) in combination with both MR imaging and MRCP.The other features included:hepatic invasion directly by gallbladder cancer in 25/32(78.12%),liver metastases in 17/19(89.47%),lymph node metastases in 28/34(82.35%),intra-adbominal distant metastases in 13/13(100%) and invasion of bile ducts in 25/26(96.15%).Conclusion MRI combined with MRCP is of significant value in diagnosing gallbladder carcinoma.

Background Diabetic retinopathy (DR) is a common ocular complication of diabetes,and its pathogenesis is associated with a variety of factors.c-Jun N terminal kinase (JNK),one of the genes involving in apoptosis,plays an important role in the pathology of diabetes,and relative research is catching increasing interests in recent years.Objective This study was to quantify the expression of JNK3 in retinas of DR murine.Methods Forty-eight SPF male C57BL/6 mice were randomly divided into the diabetes group and the normal control group.Diabetic mouse models were establishend by intraperitoneal injection of 1％ streptozocin (STZ) dissolved by sodium citrate buffer,and equvilant volume of sodium citrate buffer was used in the same way in the mice of the control mice.The left eyeballs were obtained 2,4,8 weeks after modeling and the retinas were collected.Real-time quantitaive PCR was perfored to detect the expression of JNK3 mRNA in retinas.The use and care of the experimental mice complied with the Administration of Experimental Animals in Kunming Medical College.Results Blood glucose levels were significantly higher in 2,4,8 weeks after modeling in the diabetic group compared with the normal control group (t=-5.675,-5.498,-5.347,all at P＜0.01).The relative expression levels of JNK3 mRNA (A value) in the retinas were significantly different between the groups at various time points (Fgroup =102.345,P＜0.05 ; Ftime =131.679,P＜ 0.05).The relative expression levels of JNK3 mRNA in the retinas were 3.21 ±0.14 and 5.43 ±O.37 in 4 and 8 weeks after modeling in the diabetic group,which were significantly elevated in comparison with the normal control group (2.54±0.42 versus 2.26±0.67) (t =4.073,23.399,both at P＜0.05).Compared with the second week and fourth week,the relative expression levels of JNK3 mRNA in the retinas in the eighth week were significantly raised in the diabetic group (t =10.756,16.857,both at P ＜ 0.05).Conclusions JNK3 expression in the retina upregulates in diabtic mice in a time-dependent manner.JNK3 is paopably involved in the pathogenesis and development of DR.

Objective To observe the effect of Bobath approach applied in early stage on hemiplegia after cerebral infarction. Methods 174 cases of cerebral infarction were treated with Bobath approach and assessed using Fugl-Meyer Assessment (FMA) and Modified Barthel Index (MBI), and the patients who accepted rehabilitation within or over a month after stroke were compared. Results After treatment the scores of FMA and MBI were improved more in the patients within a month than those over a month(both P<0.01). Conclusion The Bobath approach is effective on the locomotor function and the activity of daily living in patients with cerebral infarction. The earlier it starts, the better the effect should be.