1.Isolation, Screening, and Identification of Actinomycetes with Antifungaland Enzyme Activity Assays against Colletotrichum dematium ofSarcandra glabra
Lisha SONG ; Ni JIANG ; Shugen WEI ; Zuzai LAN ; Limei PAN
Mycobiology 2020;48(1):37-43
A serious leaf disease caused by Colletotrichum dematium was found during the cultivationof Sarcandra glabra in Jingxi, Rong’an, and Donglan Counties in Guangxi Province, whichinflicted huge losses to plant productivity. Biological control gradually became an effectivecontrol method for plant pathogens. Many studies showed that the application of actinomycetesin biological control has been effective. Therefore, it may be of great significance tostudy the application of actinomycetes on controlling the diseases caused by S. glabra.Strains of antifungal actinomycetes capable of inhibiting C. dematium were identified, isolatedand screened from healthy plants tissues and the rhizospheres in soils containing S.glabra. In this study, 15 actinomycetes strains were isolated and among these, strains JT-2F,DT-3F, and JJ-3F, appeared to show antagonistic effects against anthracnose of S. glabra.The strains JT-2F and DT-3F were isolated from soil, while JJ-3F was isolated from plantstems. The antagonism rate of strain JT-2F was 86.75%, which was the highest value amongthe three strains. Additionally, the JT-2F strain also had the strongest antagonistic activitywhen the antagonistic activities were tested against seven plant pathogens. Strain JT-2F isable to produce proteases and cellulase to degrade the protein and cellulose componentsof cell walls of C. dematium, respectively. This results in mycelia damage which leads toinhibition of the growth of C. dematium. Strain JT-2F was identified as Streptomyces tsukiyonensisbased on morphological traits and 16S rDNA sequence analysis.
2.Isolation, Screening, and Identification of Actinomycetes with Antifungaland Enzyme Activity Assays against Colletotrichum dematium ofSarcandra glabra
Lisha SONG ; Ni JIANG ; Shugen WEI ; Zuzai LAN ; Limei PAN
Mycobiology 2020;48(1):37-43
A serious leaf disease caused by Colletotrichum dematium was found during the cultivationof Sarcandra glabra in Jingxi, Rong’an, and Donglan Counties in Guangxi Province, whichinflicted huge losses to plant productivity. Biological control gradually became an effectivecontrol method for plant pathogens. Many studies showed that the application of actinomycetesin biological control has been effective. Therefore, it may be of great significance tostudy the application of actinomycetes on controlling the diseases caused by S. glabra.Strains of antifungal actinomycetes capable of inhibiting C. dematium were identified, isolatedand screened from healthy plants tissues and the rhizospheres in soils containing S.glabra. In this study, 15 actinomycetes strains were isolated and among these, strains JT-2F,DT-3F, and JJ-3F, appeared to show antagonistic effects against anthracnose of S. glabra.The strains JT-2F and DT-3F were isolated from soil, while JJ-3F was isolated from plantstems. The antagonism rate of strain JT-2F was 86.75%, which was the highest value amongthe three strains. Additionally, the JT-2F strain also had the strongest antagonistic activitywhen the antagonistic activities were tested against seven plant pathogens. Strain JT-2F isable to produce proteases and cellulase to degrade the protein and cellulose componentsof cell walls of C. dematium, respectively. This results in mycelia damage which leads toinhibition of the growth of C. dematium. Strain JT-2F was identified as Streptomyces tsukiyonensisbased on morphological traits and 16S rDNA sequence analysis.
3.Effects of controlled atmosphere storage on fruit quality of Siraitia grosvenorii during storage.
Lingyun WAN ; Xiaojun MA ; Changming MO ; Jiaye LAI ; Hong LUO ; Shugen WEI
China Journal of Chinese Materia Medica 2011;36(21):2937-2940
UNLABELLEDTo explore the condition of CA storage for fruit of Siraitia grosvenorii.
METHODThe CA storage of 5% CO2, 15% CO2, 30% O2 under normal temperature was applied in the experiments, and the results were compared.
RESULTThe results showed that CA storage condition could alleviate the reduction of weight loss and water content, the best CA storage condition were 5% CO2, which alleviates the reduction of firmness and increases of soluble solid content during the 45 day period.
Atmosphere ; analysis ; Cucurbitaceae ; chemistry ; Food Preservation ; methods ; Fruit ; chemistry ; Time Factors
4.Inspection on result of artemisinin content rank distribution in Guangxi.
Zhentao FAN ; Xiaojun MA ; Shixin FENG ; Shugen WEI ; Xiao WEI ; Lanping GUO ; Luqi HUANG
China Journal of Chinese Materia Medica 2009;34(3):269-271
OBJECTIVEIn order to enhance the yield of artemisinin, makes out the Artemisia annua adaptive area regional assignment in Guangxi. To ensure the nicety in study, on the base of literature study and experience on the spot, the article inspect the division result.
METHODBy document analysis and colleted data of A. annua, make out sample collect proceed and inspect the result of artemisinin content rank distribution in Guangxi.
RESULT AND CONCLUSIONResult of A. annua regional assignment is checked out in the article, the result passes the check by AQL (32, 4). The conclusions insure subsequence study and the A. annua sample collect. The result of artemisinin content rank distribution in Guangxi can be used in artemisinin production.
Antimalarials ; analysis ; pharmacology ; Artemisia annua ; chemistry ; Artemisinins ; analysis ; pharmacology ; China ; Plant Extracts ; analysis ; pharmacology
5.Effects of cinepazide maleate injection on blood pressure in patients with acute ischemic stroke and hypertension
Huisheng CHEN ; Yi YANG ; Jun NI ; Guofang CHEN ; Yong JI ; Fei YI ; Zhuobo ZHANG ; Jin WU ; Xueli CAI ; Bei SHAO ; Jianfeng WANG ; Yafang LIU ; Deqin GENG ; Xinhui QU ; Xiaohong LI ; Yan WEI ; Shugen HAN ; Runxiu ZHU ; Jianping DING ; Hua LYU ; Yining HUANG ; Yonghua HUANG ; Bo XIAO ; Tao GONG ; Xiaofei YU ; Liying CUI
Chinese Journal of Internal Medicine 2022;61(8):916-920
Objective:To investigate the blood pressure change in patients with acute ischemic stroke (AIS) and hypertension treated with cinepazide maleate injection.Methods:This was a subgroup analysis of post-marketing clinical confirmation study of cinepazide maleate injection for acute ischemic stroke: a randomized, double-blinded, multicenter, placebo-parallel controlled trial, which conducted in China from August 2016 to February 2019. Eligible patients fulfilled the inclusive criteria of acute anterior circulation ischemic stroke with National Institutes of Health Stroke Scale (NIHSS) scores of 7-25. The primary endpoints were mean blood pressure of AIS patients treated with cinepazide maleate or control, which were assessed during the treatment period (14 days), and the proportion of the patients with normal blood pressure was analyzed after the treatment period. Furthermore, a subgroup analysis was performed to investigate a possible effect of the history of hypertension on outcomes.Results:This analysis included 809 patients with hypertension. There was no significant difference in patients blood pressure and the proportion of patients with normal blood pressure (60.5% vs. 59.0%, P>0.05) between cinepazide maleate group and control group. Conclusion:Administration of cinepazide maleate injection does not affect the management of clinical blood pressure in patients with AIS.