1.The Expression of IL-8 mRNA, TSP-1 mRNA and Their Clinicopathologic Value in the Breast Cancer
Shugen LI ; Xingming YIN ; Yanchun LI
Journal of Chinese Physician 2001;0(07):-
Objective To study the expressions of IL-8 mRNA, TSP-1 mRNA and their clinicopathologic significance in the breast cancer. Methods The expressions of IL-8 mRNA, TSP-1mRNA were detected by in situ hybridization technique on the 10% formalin-fixed and routinely paraffin-embedded sections of 58 breast cancer tissues and 15 lobular hyperplastic tissues of mammary gland. Results The positive rate of IL-8mRNA was significantly higher in the breast cancer than that of lobular hyperplasia (56 9% vs 13 3%,P
2.The study of cause of early death and a matched study for the risk factors in patients undergoing maintenance hemodialysis
Jincheng HUA ; Meng LIANG ; Shuqiong SHEN ; Caifeng LI ; Shugen XU
Chinese Critical Care Medicine 2015;(5):354-358
ObjectiveTo explore the cause of early death (death within 3-12 months after hemodialysis) and the related influencing factors patients undergoing maintenance hemodialysis (MHD) as to provide a scientific basis for the prevention of early death.Methods A retrospective matched controlled study was conducted. Fifty-one patients who underwent MHD from January 2004 to April 2014 and died within 3-12 months after hemodialysis in hemodialysis center of the 174th Chinese People's Liberation Army Hospital were included in the case group by retrospective analysis method. According to 1∶2 matched controls, 102 patients underwent hemodialysis in the same period (±2 months) and survived over 12 months were selected as control group. All patients received regular hemodialysis (dialysis 2-3 times per week), with conventional limitation of water and sodium intake, routine treatments such as control of blood pressure, treatment of anemia and disorders of calcium and phosphorus contents. Causes of short-term death were analyzed. Clinical and biochemical parameters of two groups were collected when dialysis was started, and the single factor and multiple factors logistic regression was used to analyze the related risk factors when dialysis was started. Receiver operating characteristic curve (ROC) was plotted to evaluate the value of above parameters in predicting the early death in patents with MHD.Results The main causes of early death of 51 patients with MHD were mainly cardiovascular and cerebrovascular diseases (27 cases, 52.9%), and infections (15 cases, 29.4%). It was shown by single factor analysis that the age [odds ratio (OR) = 6.625, 95% confidence interval (95%CI) = 3.232-13.580,P = 0.000], diabetes (OR = 3.875, 95%CI = 0.654 - 10.622,P = 0.031), specialist intervention time before dialysis (OR = 0.349, 95%CI =0.287 - 0.572,P = 0.004), the emergence of cardiovascular and cerebrovascular events before dialysis (OR = 9.667, 95%CI = 4.632 - 20.174,P = 0.000), the first dialysis for emergency dialysis (OR = 3.875, 95%CI = 1.713 - 8.765, P = 0.005), blood albumin level (OR = 0.294, 95%CI = 0.068 - 0.550,P = 0.008), leukocyte count (OR = 6.286, 95%CI = 1.648 - 23.982,P = 0.026), neutrophil count (OR = 2.833, 95%CI = 1.630 - 4.923,P = 0.001) might be the factors correlating with early death. Eight independent factors were statistically significant, and their effect on the MHD patients was analyzed by logistic regression analysis inα = 0.05 level. The results showed that patients with old age (OR = 1.054, 95%CI = 1.019-1.090,P = 0.002), and the emergence of cardio-cerebrovascular events (OR = 7.469, 95%CI = 2.474 - 22.545,P = 0.000)were early death risk factors of MHD patients, and early specialist intervention before dialysis was a protective factor (OR = 0.286, 95%CI = 0.113-0.722,P = 0.008). ROC curve showed that age had moderate diagnostic value for early death of MHD [area under ROC curve (AUC) = 0.756], the cut-off value was 59.0 years old, the sensitivity was 66.7%, and the specificity was 77.5%. The diagnostic value of early specialist intervention before dialysis was relatively low (AUC = 0.367), the cut-off value was 0.875 years, the sensitivity was 39.2%, and the specificity was 33.3%.Conclusion Old age, the emergency of cardiovascular and cerebrovascular events before dialysis is associated with early death, and specialist intervention ahead of dialysis can reduce the risk of early death.
3.The protective effect of bioimpedance spectroscopy guided ultrafiltration on residual renal function in hemodialysis patients
Yangyang WANG ; Caifeng LI ; Yuqing HU ; Meng LIANG ; Shugen XU
Chinese Journal of Disease Control & Prevention 2017;21(9):939-942
Objective To observe the protective effect of bioimpedance spectroscopy guided ultrahltration on residual renal function in new hemodialysis patients.Methods Patients with end-stage renal disease recruited from January 2015 to June 2016,were randomly divided into experimental group and control group.And all the patients were followed up for 3 months.The ultrafiltration was guided by the bioimpedance spectroscopy analysis in the experimental group,while the ultrafiltration was based on the edema,blood pressure,symptoms of low blood pressure and the increase of weight during the hemodialysis interphase in the control group.The difference of residual renal function,24 hours urine volume and the incidence rate of adverse events between the two groups were collected.Results Compared with the control group,the urine volume(932.58 ± 230.16 ml vs 584.45 ± 137.76 ml,t =7.226,P < 0.001) and residual renal function (RRF) (4.55 ± 0.90 ml/min vs 3.08 ±0.68 ml/min,t =7.300,P <0.001)in the experimental group were higher.The drop of RRF(3.14 ±2.05 ml/min vs 4.40 ±2.09 ml/min,t =-2.384,P =0.020) and urinary volume (452.58 ±456.96 ml vs 877.45 ± 452.45 ml,t =-3.679,P =0.001) were lower in the experimental group.While there was no significant difference in the incidence of adverse events between the two groups (t =2.081,P =0.084).Conclusions It is helpful for slowing down the decline of residual renal function by using the bioimpedance spectroscopy guided ultrafiltration.
4.The Clinical Observation on Treatment of Stable Cardiac Angina Pectoris due to Qi-deficiency and Blood-stasis with Yiqi Huoxue Jiedu Decoction
Shugen DING ; Zhongzhi LI ; Qianglong XIE ; Yebin HU
International Journal of Traditional Chinese Medicine 2008;30(4):268-269
Objective To investigate the effects of Yiqi Huoxue Jiedu Decoction on Syndrome,hemorheology and homocysteine(Hcy)of patients with stable cardiac angina pectoris due to qi-deficiency and blood-stasis.Methods 98 patients with stable angina pectoris due to qi-deficiency and blood-stasis are randomly divided into a treatment group and a control groups.Conventional treatments as inhaling oxygen,dilating coronary artery,and resisting coagulation were used for the both group.Besides conventional treatments.Yiqi Huoxue Jiedu Decoction was additionally given to the treatment group.Observe the changes of Syndrome,hcmorheology and homocysteine.Results The improvement of Syndrome,hemorheology and homocysteine in the treatment group was significantly better than the Control group(P<0.01 or P<0.05).Conclusion The curative effect of treating stable a.gina pectoris due to qi-deficiency and blood-stasis with conventional treatments together with Yiqi Huoxue Jiedu Decoction is better than conventional western medical treatment.
5.STATICS ANALYSIS AND OPENGL BASED 3D SIMULATION OF COLLABORATIVE RECONFIGURABLE PLANETARY ROBOTS
Zheng ZHANG ; Shugen MA ; Bin LI ; Liping ZHANG ; Binggang CAO
Journal of Pharmaceutical Analysis 2006;18(2):102-105,117
Objective To study mechanics characteristics of two cooperative reconfigurable planetary robots when they get across an obstacle, and to find out the relationship between the maximum height of a stair with the configuration of the two-robot, and to find some restrictions of kinematics for the cooperation. Methods Multirobot cooperation theory is used in the whole study process. Inverse kinematics of the robot is used to form a desired configuration in the cooperation process. Static equations are established to analyze the relations between the friction factor, the configuration of robots and the maximum height of a stair. Kinematics analysis is used to find the restrictions of the two collaborative robots in position, velocity and acceleration. Results 3D simulation shows that the two cooperative robots can climb up a stair under the condition of a certain height and a certain friction factor between robot wheel and the surface of the stair. Following the restrictions of kinematics, the climbing mission is fulfilled successfully and smoothly. Conclusion The maximum height of a stair, which the two cooperative robots can climb up, is involved in the configuration of robots, friction factor between the stair and the robots. The most strict restriction of the friction factor does not appear in the horizontal position. In any case, the maximum height is smaller than half of the distance between the centroid of robot1 with the centroid of robot2. However, the height can be higher than the radius of one robot wheel, which profit from the collaboration.
6.Genotyping of Mycobacterium tuberculosis isolates circulating in Qinghai province by spoligotyping.
Zhaofen WANG ; Email: KRISTY538@163.COM. ; Bin LI ; Yongcheng MA ; Mingxia JIANG ; Xiaodong SU ; Binzhong MA ; Shou LIU ; Shugen QU ; Ze ZHOU ; Xiaojuan ZHANG
Chinese Journal of Preventive Medicine 2015;49(6):565-567
7.Effects of cinepazide maleate injection on blood pressure in patients with acute ischemic stroke and hypertension
Huisheng CHEN ; Yi YANG ; Jun NI ; Guofang CHEN ; Yong JI ; Fei YI ; Zhuobo ZHANG ; Jin WU ; Xueli CAI ; Bei SHAO ; Jianfeng WANG ; Yafang LIU ; Deqin GENG ; Xinhui QU ; Xiaohong LI ; Yan WEI ; Shugen HAN ; Runxiu ZHU ; Jianping DING ; Hua LYU ; Yining HUANG ; Yonghua HUANG ; Bo XIAO ; Tao GONG ; Xiaofei YU ; Liying CUI
Chinese Journal of Internal Medicine 2022;61(8):916-920
Objective:To investigate the blood pressure change in patients with acute ischemic stroke (AIS) and hypertension treated with cinepazide maleate injection.Methods:This was a subgroup analysis of post-marketing clinical confirmation study of cinepazide maleate injection for acute ischemic stroke: a randomized, double-blinded, multicenter, placebo-parallel controlled trial, which conducted in China from August 2016 to February 2019. Eligible patients fulfilled the inclusive criteria of acute anterior circulation ischemic stroke with National Institutes of Health Stroke Scale (NIHSS) scores of 7-25. The primary endpoints were mean blood pressure of AIS patients treated with cinepazide maleate or control, which were assessed during the treatment period (14 days), and the proportion of the patients with normal blood pressure was analyzed after the treatment period. Furthermore, a subgroup analysis was performed to investigate a possible effect of the history of hypertension on outcomes.Results:This analysis included 809 patients with hypertension. There was no significant difference in patients blood pressure and the proportion of patients with normal blood pressure (60.5% vs. 59.0%, P>0.05) between cinepazide maleate group and control group. Conclusion:Administration of cinepazide maleate injection does not affect the management of clinical blood pressure in patients with AIS.
8.Study on VNTR diversity of clinical Mycobacterium tuberculosis isolates from Qinghai.
Bin LI ; Haican LIU ; Zhaofen WANG ; Email: KRISTY538@163.COM. ; Yongcheng MA ; Xiaodong SU ; Mingxia JIANG ; Kanglin WAN ; Shou LIU ; Xiuqin ZHAO ; Shugen QU
Chinese Journal of Epidemiology 2015;36(10):1158-1161
OBJECTIVETo investigate the variable number tandem repeats (VNTR) genetic polymorphisms, genotyping and distribution pattern of clinical Mycobacterium (M.) tuberculosis isolates from Qinghai province.
METHODSThe clinical M. tuberculosis strains isolated from the patients with tuberculosis and related background data were collected from Qinghai Provincial Center for Disease Control and Prevention from 2009 to 2012. Genotyping was conducted by using multiple locus VNTR analysis (MLVA). Genomic DNA was extracted and 15 VNTR loci were amplified with PCR and the PCR products were detected with gel electrophoresis. The VNTR diversity and clusters of genotyping were analyzed with BioNumerics (Version 5.0).
RESULTSA total of 251 clinical M. tuberculosis isolates were analyzed with 15 VNTR loci showing that there were great genetic diversity in these isolates. Six of the 15 VNTR loci, showed that the Hunter-Gaston index (HGI) were higher than 0.6, in which the highest resolution was MIRU26. The clusters of genotyping showed that these isolates could be categorized into four gene clusters and 238 genotypes. The four gene clusters accounted for 4.9%, 91.9%, 1.6% and 1.6% of the clinical isolates, respectively.
CONCLUSIONThe results showed that there is great variety of VNTR genetic polymorphisms in clinical M. tuberculosis isolates in Qinghai province.
China ; DNA, Bacterial ; genetics ; Genetic Variation ; Genotype ; Humans ; Minisatellite Repeats ; Mycobacterium tuberculosis ; genetics ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Sequence Analysis, DNA ; Tuberculosis ; microbiology