Negative pressure aspirator is necessary to every clinical sections. Automatic wash of negative pressure aspirator is completed by applying the technology of liquid level induction. This development eases the work intensity of medical care personnel, lowers the medical treatment cost, cuts down the infection probability to medical care personnel in and reduces the failure rate.

Objective To comfort patients, alleviate nurses and the family member's labor, and accommodate the relationship of doctors and patients. Methods To refit a common controlled sickbed by hands to a sickbed which can rise and fall automatically by electric power. Results According to the straightly driven screw, patients can control comfortable a pose by keyboard. Conclusion It is easy to refit. The cost is low while the effect is desirable.

Excessive activation of renal sympathetic nerves may be a key factor for occurrence and maintenance of hypertension.Renal sympathetic nerve ablation is a selective sympathetic denervation therapy,Which provides neW route for antihypertensive treatment.This article made an overvieW on further expansion of safety,effectiveness and indications of renal sympathetic nerve ablation treatment for hypertension.

Objective To improve the surgical technique to establish a simple model of heterotopic segmental small bowel transplantation in rats. Methods The segmental intestine was harvested with "non-injury"technique and consisted of donor aortic segment with superior mesenteric artery and portal vein, after perfused in situ with lactated Ringer's solution, the graft was stored in 4℃ lactated Ringer's solution for about 1 h. The heterotopic segmental intestine transplantation was performed by end-to-side anastomosis of donor aorta to recipient aorta. The left kidney of the recipient was resected and the donor's portal vein was anastomosed to the recipient left renal vein by" cuff anastomosis". Results A total of 87 intestine transplantations were performed, 26 of them was regarded as formal experiment group.The average time for the arterial and venous anastomosis were 25～30min and 5 min.Among the 26 recipient rats,21 survived more than 3days.The average survival was 8.93?2.59 days, The longest survival was 14 days in this group. Conclusions It is indicated that the keys of success of small intestinal transplantation are as follows:the high quality graft,excellent anastomosis of blood vessels and adequate liquid supplement.

Objective To determine the changes of gene expression profile in small intestinal allograft in rats after ischemic preconditioning(IPC),and to study the mechanism of graft protection of IPC.Methods Rats are randomly divided into 3 groups:sham operation(S group),small bowel transplant(SBT group)and IPC small bowel transplant group(ISBT group).Total RNAs was extracted from intestine of the 3 groups 1h after the intestine remored and cold preservation/reperfusion,and then purified to mRNA.mRNAs was then reversely transcribed to cDNA and to prepare hybridization probes.The mixed probes were hybridized to the cDNA microarray.After high-stringent washing,the fluorescent signals on cDNA microarray were scanned and analyzed.Results Among the 4 096 target genes,297 differentially expressed genes were identified between normal intestine and intestinal allograft in ISBT group;among those 84 genes which have been reported,including 18 genes expressing down and 66 genes expressing up regulation.Differentially expressing genes could be related to the protective effect of IPC.Conclusions The mechanisms of protective effect of IPC on cells of the graft are by modulation of genes related to cell adhesion,related to cellular energy and metabolism,and related to the signal transmission of the cells.

Objective\ To introduce the clinical application of the osteocutaneous flap pedicled with the lateral superior genicular artery or the lateral most superior genicular artery in reconstruction of defect of the soft tissue, bone or compound tissue defect on the hand or distal of the forearm.Methods\ The origin course distribution and an astomosis of the lateral superior genicular artery and the lateral most superior genicular artery were observed on the lower limbs of 30 adult cadavers perfused with pink plastics. The osteocutaneous flap based on the lateral superior genicular artery or the lateral most superior genicular artery were designed according to anatomic study. Results\ One cutaneneous flap, 1 osteo flap and 3 osteocutaneous flaps pedicled with lateral superior genicular artery were used; the area of the cutaneous or osteo flap were 7.5 cm?5.0 cm-14.5 cm?6.5 cm and 5.0 cm?1.5 cm?1.2 cm-6.5 cm?2.0 cm?1.6 cm respectively; all the flap and osteocutaneous flap survived. Bone union occurred 3-5 months after the operation. Conclusion\ The lateral supragenual osteocutaneous flap can be used to reconstruct the defect of soft tissue, bone or compound tissue defect on the hand and distal of the forearm.

Objective To observe the outcome of healthy C7 nerve root transfer through the anterior spinal approach to repair the brachial plexus avulsion. Methods The healthy C7 nerve root was sectioned at the junction site of trunk and division, the proximal side was dissected to the foramina and transfered behind the posterior aspect of anterior scalenus to the space between the esophagus and cervical vertebrae, then the 4-6 bands of cutaneous nerves were used for bridging the defect between the healthy C7 and recipient nerve to repair the upper trunk or dorsal division of the upper trunk of the injured brachial plexus. From March 2002 to August 2003, 21 patients with brachial plexus avulsion were repaired using this procedure, among which 14 patients (12 males, 2 females) were followed up for more than 12 months. The average age was 31 years with a range of 17 to 41 years. The interval from injury to operation ranged from 2 to 6 months with a mean of 4 months. 14 patients included 5 cases with total brachial plexus avulsion, 8 cases with upper and middle trunk avulsion associated with partial injury of the lower trunk and 1 case with cutting injury of upper and middle trunk. Results The average follow-up duration was 16 months ranging from 12 to 19 months. 9 of the 10 cases who underwent the healthy C7 transfer through the anterior spinal approach to repair the upper trunk, the C5 and C6 nerve roots or the anterior and posterior division of upper trunk achieved at least the MRC grade 3 motor recovery of shoulder abduction and elbow flexion and 1 case failed. 3 cases underwent the healthy C7 transfer to repair the dorsal division of the upper trunk achieved at least the MRC grade 3 motor recovery of shoulder abduction. 1 case who underwent the healthy C7 transfer to repair the C6 nerve root didnt get recovery. Conclusion The result of healthy C7 transfer througth the anterior spinal approach to repair the upper trunk of brachial plexus for restoration of the shoulder abduction and elbow flexion or the function of shoulder abduction in repairing the dorsal division of upper trunk is satisfactory.

0.05). The 5-year survival rate of cyclinD1-posit ive patients was significantly lower than that of cyclinD1-negative patients ( P

Objective To observe the results of free medialis pedis mini-flap pedicled on medial branch of deep branch of the medial plantar artery to repair a soft tissue defect of finger. Methods The mini-flap designed on the medial side of the foot pedicled on the medial branch of deep branch of medial plantar artery was used to repair the soft tissue defect of finger or the degloved injury of the finger combined with second toe flap, furthermore, the vascular pedicle of flap was anastomosed with the digital artery and dorsal vein respectively. 13 fingers of 11 cases, which included 10 fingers of 8 cases of male and 3 fingers of 3 cases of female with an average of 32 years ranging from 18 to 58 years, were performed 13 free medialis pedis mini-flaps transplantation. Eight free medialis pedis mini-flaps were used to repair the soft tissue defect of the finger, and 5 free medialis pedis mini-flaps combined with the second toe composite flap were used to reconstruct the degloved finger. Results All the patients were followed up on an average of 21 months ranging from 3 months to 5.5 years. The maximal area of flaps was 5 cm?4 cm, and the minimal was 2.5 cm?3 cm. 12 of the 13 free medialis pedis mini-flaps were survived completely, however, 1 healed but partly necrosis. The color, character and thickness of this flap after transplantation were similar to the skin of the finger; and the appearance of the reconstructed finger by mini-medialis pedis flap and second toe composite flap was perfect. The transplanted skin of donated areas survived well, and the appearance and functions of donated feet were not affected significantly. Conclusion The free medialis pedis flap pedicled on medial branch of the deep branch of the medial plantar artery was one of the optimal flaps to repair soft tissue defect of the finger especially on the palmer side.

Objective To study the early language development for children with cleft lip and palate. Methods 105 non-intellectual and hearing impairment children who has done cleft palate operation, accepted one-to-one treatment mode for speech rehabilitation, once or twice a week, 30~45 min each time. Results The speech articulation of all the children improved (P<0.001). Conclusion Early surgery can restore the palatopharyngeal closure function and get better voice as soon as possible.