The paper studied effect of different doses of Tanshinone on human polymorphonuclear leucocyte chemokinesis by agarose microdroplet assay. The results indicate that more than 10ng/ml Tanshinone inhibites significantly human polymorphonuclear leucocyte chemokinetic response to 10~(-9)M FMLP. This inhibitory effect is probably one of mechanisms of Tanshinone anti-inflammatory activity. The agarose microdroplet assay is a reliable mothod for studying leucocyte chemokinesis in vitro.

OBJECTIVE To discuss nursing department′s function in preventing hospital infection management in order to improve its level. METHODS Nursing department actively works with hospital infection management department to establish organization,consummate system,carry out measure,regulate nursing technique operation procedure and nurse conduct norm,and train the nursing staff. RESULTS The rate of hospital infection has been descended gradually from 2.29% in 2000 to 1.01% in 2005. CONCLUSIONS Nursing department is performing important function in preventing hospital infection management work.

Objective: To investigate rules and affect factors in virus hepatitis complicated with biliary system damage. Methods: To observe 158 patients with various clinical types of viral hepatitis, gallbladder filling degree of gallbladder wall thickness, the gallbladder bile in the gallbladder situation, through sound situation by ultrasound, and the patients with intrahepatic, extrahepatic bile duct wall thickness were measured, the clarity of cavity were observed. Results:The 158 cases of patients with biliary ultrasonic testing results showed the gallbladder wall thickening, rough bilateral syndrome, sound transparent difference, stones, gallbladder enlargement or smaller, like solid. Severe hepatitis, biliary system in patients and liver cirrhosis was significantly higher than that of the abnormal in patients with acute hepatitis and chronic hepatitis (x2=17.96, x2=16.27; P<0.05). Conclusion: Viral hepatitis complicated with biliary system damage is related to clinical type, course of disease, liver function damage. Observe the change of ultrasonic dynamic biliary system, liver function testing timely, and provide the basis for guiding clinical treatment, to judge the severity and chronicity of hepatitis prediction.

Objective To explore the mechanism of quercetin against the toxicity of amyloid β(Aβ)to neuroblastoma cell SK -N -SH.Methods In the experiment,SK -N -SH cells were divided to four groups,control group,Aβgroup,Aβ+quercetin group and quercetin group,and treated for 72h.The proliferation of cells,the concen-trations of reactive oxygen species (ROS)and glutathione (GSH)in cells were determined.Results Compared with the control group,Aβ[AβgrouP =(50.6 ±2.5)]could significantly suppress the proliferation of neuroblastoma cells (P =0.000).However,Aβgroup added with quercetin[Aβ+quercetin =(88.0 ±9.1)]showed decreased cellular toxicity induced by Aβ(P =0.001).Additionally,there was no significant difference between the control group and quercetin group.Likely,the concentration of ROS increased in Aβgroup (523.3 ±22.5 ),which was higher than (190.3 ±25.7)in the control group and (224.0 ±56.0)in quercetin group (P =0.000).At last,quercetin could increase GSH level in SK -N -SH cells and higher than Aβgroup[(3.0 ±0.2),P =0.004]and Aβ+quercetin [(5.6 ±1.1),P =0.008].Conclusion Quercetin could attenuate the suppression of proliferation of SK -N -SH cells induced by Aβ,that might be though increased concentration of GSH and decreased level of ROS.These data suggested that quercetin has clinical potentials in neurology.

‘Typhoid fever and Miscellaneous Diseases’is well known as‘the origin of prescription’. Prescriptions in this book are reliable, exquisite and rigorous, can cure hard diseases using very few kinds of TCM. The principle of‘corresponding prescription to symptom’makes proved cases simple but highly effective to complex illnesses. This thesis introduced four proved cases of remedying miscellaneous internal medicine diseases under the guidance of determination of treatment based on syndrome differentiation, principle of correspondence of prescription and syndrome, the author used classic prescriptions, such as Wuzhuyu Decoction, Guizhi Shaoyao Zhimu Decoction, Guizhi and Houpo Xizi Decoction and Dachaihu Decocion.

Minimal residual disease (MRD) is the cause of relapse in leukemia,and its dynamic monitoring has been a hotspot yet proven to be difficult.MRD in acute B-lineage lymphoblastic leukemia has its own characteristics,normal bone marrow produces a large number of B precursor cells,easily mistaken for leukemia cells,and there were no specific gene alterations that can be conveniently detected.This paper summarizes the antibody combinations in flow cytometry,timing for surveillance and polymerase chain reaction for gene detection,and provide a reference for B lymphocytic leukemia MRD monitoring.

Objective To improve the ability of rheumatologist to diagnose systemic lupus erythematosus (SLE) complicated with ureterohydronephrosis by analyzing the characteristics of clinical manifestations.Methods Patients with ureterohydronephrosis hospitalized in Peking Union Medical College Hospital between 2000 to 2008 were analyzed retrospectively. The clinical characteristics, serological findings, treatment and prognosis of these patients were reviewed. Comparisons between the groups were performed with X2 test and t-test. Results SLE patient with ureterohydronephrosis accounted for 1.26% of the SLE patients hospitalized in the same period. Twenty-eight patients presented with gastrointestinal symptoms, 14 patients suffered from bladder irritative symptoms. Nineteen patients were with bilateral ureterohydronephrosis, and 8 patients were with unilateral ureterohydronephrosis. Fourteen patients had stype positive ANA, 14 patients had positive antiSSA antibodies. All patients were treated with steroid and immune suppressive therapy, 11 patients were cured, while 3 patients had no improvement. Conclusion Ureterohydronephrosis isn't a very rare complication of SLE. SLE patients with ureterohydronephrosis often present with gastrointestinal symptoms and have high incidence of chronic intestinal pseudo obstruction. High ratio of stype ANA antibody and high positive rate of anti-SSA are most important characteristics in this subtype of SLE patients. The complications can be reversed if the patients are treated early and appropritaely.

Objective　In order to understand situation and need s for health education of AIDS prevention in high school students in Anhui. Methods　1 617 students from 12 schools in 4 prefectures were sur veyed with questionnaires. Results　More than 50% students got the i nformation from newspaper, broadcasting, TV. Only 7.5% and 4.7% st udents acquired the info rmation from the subject lectures at school level and class level respectively. 14.4% students attended health education activities f or AIDS prevention. 10.9% students received relevant pro paganda materials, 77.8% students had never seen condom, 44.1% students knew the function of condom, and 8.6% students approved of premarital sexual life, of wh ich , ratio of male students were significantly higher than that of female students. More than 70% students were willing to care and help patients with HIV infectio n. Conc lusions　It is necessary and possible to develop health education for AI DS prevention in high school students. It is an important measure to develop hea lth education to block HIV transmission.

Objective To investigate the association between human leukocyte antigen DRB1gene (HLA-DRB1) polymorphisms and asthma among Mongolian and Han peoples in China. Methods Sequence-specific primer polymerase chain reaction (PCR-SSP) was used to detect frequencies of HLA DRB1 alleles in 45 Han and 45 Mongolian patients of asthma, as well as 46 healthy Han and 45 healthy Mongolian people as controls. Frequencies of each locus of HLA-DRB1 gene were calculated and odds ratio (OR) for each locus was estimated and tested by chi-square test. Results Frequency of HLA-DR9 allele was significantly higher in Han ( 13.33 %, OR of 7. 82) and Mongolian ( 16.67 %, OR of 4. 10) asthmatic patients, as compared to Han ( 2. 22 % ) and Mongolian ( 5.43 % ) controls, respectively ( P < 0. 05 ) ;frequency of HLA-DR11 allele in Mongolian asthmatic patients ( 10. 00 % ) was significantly higher than that in healthy Mongolians ( 1.11% ) ( P < 0. 05, OR of 11.00 ) ;and frequency of HLA-DR7 allele in Han asthmatic patients was significantly lower than that in healthy Han people ( P < 0. 05, OR of 0. 08 ). Frequency of HLA-DR7 allele in Han asthmatic patients (1.11% ) was significantly lower than that in Mongolian patients (10. 87 % ). Conclusions HLA-DR9 allele is a common in the people of Inner Mongolian Autonomous Region, and associated with susceptibility of asthma in Han and Mongolian peoples. HLA-DR11 allele is a unique susceptive gene for Mongolian asthmatic patients and HLA-DR7 allele is a protecting one for asthma in Han people.

Objective To investigate the serum lipoprotein (Lp) (a) level in patients with acute ischemic stroke and the prediction for short-term functional outcome. Methods The levels of serum Lp(a) were measured in 210 patients with acute ischemic stroke (within 24 h after onset) and 100 healthy people matched the gender and age. The patients were assessed with National Institutes of Health Stroke Scale (NIHSS) as admission, and modified Rankin Scale (mRS) as discharge. Results The median of Lp(a) was 325 mg/L (IQR: 180~545) in patients,significantly more than 148 mg/L (IQR: 69~265) in the normal controls (P<0.001). Level of serum Lp(a) was an independent variable for functional outcome (OR=1.004, 95%CI: 1.001~1.007, P<0.001) and death (OR=1.006, 95%CI: 1.001~1.008, P<0.001) in patients. In the receiver operating characteristic curve, the area under curve was more in the combined model [Lp(a) and NIHSS] than the NIHSS alone.Conclusion Serum Lp(a) increased in the in patients with acute ischemic stroke, and may predict the short-term outcome independently.