The paper studied effect of different doses of Tanshinone on human polymorphonuclear leucocyte chemokinesis by agarose microdroplet assay. The results indicate that more than 10ng/ml Tanshinone inhibites significantly human polymorphonuclear leucocyte chemokinetic response to 10~(-9)M FMLP. This inhibitory effect is probably one of mechanisms of Tanshinone anti-inflammatory activity. The agarose microdroplet assay is a reliable mothod for studying leucocyte chemokinesis in vitro.

Objective: To investigate rules and affect factors in virus hepatitis complicated with biliary system damage. Methods: To observe 158 patients with various clinical types of viral hepatitis, gallbladder filling degree of gallbladder wall thickness, the gallbladder bile in the gallbladder situation, through sound situation by ultrasound, and the patients with intrahepatic, extrahepatic bile duct wall thickness were measured, the clarity of cavity were observed. Results:The 158 cases of patients with biliary ultrasonic testing results showed the gallbladder wall thickening, rough bilateral syndrome, sound transparent difference, stones, gallbladder enlargement or smaller, like solid. Severe hepatitis, biliary system in patients and liver cirrhosis was significantly higher than that of the abnormal in patients with acute hepatitis and chronic hepatitis (x2=17.96, x2=16.27; P<0.05). Conclusion: Viral hepatitis complicated with biliary system damage is related to clinical type, course of disease, liver function damage. Observe the change of ultrasonic dynamic biliary system, liver function testing timely, and provide the basis for guiding clinical treatment, to judge the severity and chronicity of hepatitis prediction.

OBJECTIVE To discuss nursing department′s function in preventing hospital infection management in order to improve its level. METHODS Nursing department actively works with hospital infection management department to establish organization,consummate system,carry out measure,regulate nursing technique operation procedure and nurse conduct norm,and train the nursing staff. RESULTS The rate of hospital infection has been descended gradually from 2.29% in 2000 to 1.01% in 2005. CONCLUSIONS Nursing department is performing important function in preventing hospital infection management work.

Pineal arteries and veins of 46 adult human brains were observed and measured with stero-microscope and light microscope.According to the number of branches, the pineal arteries may be classified into four types: single branched, double branched, three branched and multiple branched types. Among them the single and double branched type occur most frequently. (in 54.55% and 34.09％ respectively), the artery originates mainly from the postcromedial choroidal artery (in 69.72％), and the next more frequent origin is the the quadrigeminal artery (in 28.87%). Some pineal arteries have double origins from abovementioned arteries.Most of the starting points of pineal arteries situate in the recess between the superior colliculus and the pineal body, and less of them on the lateral side of the colliculus or beneath the splenium of the corpus callosum. The artery runs frequently forwards or medially towards the gland and enters it at the middle part of its side. Then it gives branches out of or in the capsule to the surface the gland. The branching patterns may be classified into four types and ten subtypes.The arteries vary in length from 0.2～38.4 mm. The mean external diameter of which is 0.30+0.08 mm, and the internal diameter,0.21?0.07mm.On the pineal body there are a pair of venous stems known as choroidal-pineal veins. They begin from the tela choroidea of the third ventricle. After accepting the dorsal and ventral pineal veins on the ventrolateral side of the pineal body, the two venous stems usually unite to form a single vein which converges into the great cerebral vein.

‘Typhoid fever and Miscellaneous Diseases’is well known as‘the origin of prescription’. Prescriptions in this book are reliable, exquisite and rigorous, can cure hard diseases using very few kinds of TCM. The principle of‘corresponding prescription to symptom’makes proved cases simple but highly effective to complex illnesses. This thesis introduced four proved cases of remedying miscellaneous internal medicine diseases under the guidance of determination of treatment based on syndrome differentiation, principle of correspondence of prescription and syndrome, the author used classic prescriptions, such as Wuzhuyu Decoction, Guizhi Shaoyao Zhimu Decoction, Guizhi and Houpo Xizi Decoction and Dachaihu Decocion.

Objective To investigate the association between human leukocyte antigen DRB1gene (HLA-DRB1) polymorphisms and asthma among Mongolian and Han peoples in China. Methods Sequence-specific primer polymerase chain reaction (PCR-SSP) was used to detect frequencies of HLA DRB1 alleles in 45 Han and 45 Mongolian patients of asthma, as well as 46 healthy Han and 45 healthy Mongolian people as controls. Frequencies of each locus of HLA-DRB1 gene were calculated and odds ratio (OR) for each locus was estimated and tested by chi-square test. Results Frequency of HLA-DR9 allele was significantly higher in Han ( 13.33 %, OR of 7. 82) and Mongolian ( 16.67 %, OR of 4. 10) asthmatic patients, as compared to Han ( 2. 22 % ) and Mongolian ( 5.43 % ) controls, respectively ( P < 0. 05 ) ;frequency of HLA-DR11 allele in Mongolian asthmatic patients ( 10. 00 % ) was significantly higher than that in healthy Mongolians ( 1.11% ) ( P < 0. 05, OR of 11.00 ) ;and frequency of HLA-DR7 allele in Han asthmatic patients was significantly lower than that in healthy Han people ( P < 0. 05, OR of 0. 08 ). Frequency of HLA-DR7 allele in Han asthmatic patients (1.11% ) was significantly lower than that in Mongolian patients (10. 87 % ). Conclusions HLA-DR9 allele is a common in the people of Inner Mongolian Autonomous Region, and associated with susceptibility of asthma in Han and Mongolian peoples. HLA-DR11 allele is a unique susceptive gene for Mongolian asthmatic patients and HLA-DR7 allele is a protecting one for asthma in Han people.

Minimal residual disease (MRD) is the cause of relapse in leukemia,and its dynamic monitoring has been a hotspot yet proven to be difficult.MRD in acute B-lineage lymphoblastic leukemia has its own characteristics,normal bone marrow produces a large number of B precursor cells,easily mistaken for leukemia cells,and there were no specific gene alterations that can be conveniently detected.This paper summarizes the antibody combinations in flow cytometry,timing for surveillance and polymerase chain reaction for gene detection,and provide a reference for B lymphocytic leukemia MRD monitoring.

Objective To explore the mechanism of quercetin against the toxicity of amyloid β(Aβ)to neuroblastoma cell SK -N -SH.Methods In the experiment,SK -N -SH cells were divided to four groups,control group,Aβgroup,Aβ+quercetin group and quercetin group,and treated for 72h.The proliferation of cells,the concen-trations of reactive oxygen species (ROS)and glutathione (GSH)in cells were determined.Results Compared with the control group,Aβ[AβgrouP =(50.6 ±2.5)]could significantly suppress the proliferation of neuroblastoma cells (P =0.000).However,Aβgroup added with quercetin[Aβ+quercetin =(88.0 ±9.1)]showed decreased cellular toxicity induced by Aβ(P =0.001).Additionally,there was no significant difference between the control group and quercetin group.Likely,the concentration of ROS increased in Aβgroup (523.3 ±22.5 ),which was higher than (190.3 ±25.7)in the control group and (224.0 ±56.0)in quercetin group (P =0.000).At last,quercetin could increase GSH level in SK -N -SH cells and higher than Aβgroup[(3.0 ±0.2),P =0.004]and Aβ+quercetin [(5.6 ±1.1),P =0.008].Conclusion Quercetin could attenuate the suppression of proliferation of SK -N -SH cells induced by Aβ,that might be though increased concentration of GSH and decreased level of ROS.These data suggested that quercetin has clinical potentials in neurology.

Clinical skills training is a key factor for improvement of the quality of clinical teaching.Developing highly specific clinical skills training specification is an important step to improve the quality of gynecologic oncology clinical teaching.Based on its importance,a'four steps training strategy,known groups-building specification-train thinking-make a request is developed and used by Dept.of Obsterics and Gynecology,the 3rd Affiliated Hospital,Kunming Medical University.It guides students to achieve the basic theory,basic knowledge and basic skills through a comprehensive way,and it also encourages students with highly learning initiative of gynecologic oncology clinical skills.On the other side,in the clinical skills training will lead teachers to develop their thinking and enhance the enthusiasm of teaching.This method provides a new sight for gynecologic oncology clinical skills training in a new manner.

OBJECTIVE To analyze the reason for delayed healing of obstetric abdomen vertical cut and discuss the preventive nursing countermeasure.METHODS The discharged cases during Jul 2005 Jun 2006 and the inpatient cases during Jul 2006 to Jun 2007 were observed and monitored and at the same time the preventive nursing countermeasure was provided and the statistic of clinic material of two years was analyzed.RESULTS A satisfying effect has been made by taking objective monitoring and preventive nursing countermeasure the rate of delayed healing of obstetric abdomen vertical cut been reduced from the original 2.75% to the current 0.93%.CONCLUSIONS The key to prevent the delayed healing of obstetric abdomen vertical cut is prevention.Taking objective monitoring and preventive nursing countermeasure play an importment role in promoting healing of obstetric abdomen vrtical cut.