Objective To develop TaqMan real-time PCR assay for detection and identification of streptococcus pneumonia iso-lated from children CAP.Methods Based on the sequences of lytA gene,primers and probe were designed and the assay was optimized.Then 1 504 sputum samples were detected by culture and the developed assay with double-blind testing.Results The lower limit of detection in the developed real-time PCR assay was 18.75 cfu/PCR,and had no cross reaction.141 strep-tococcus pneumonia strains were detected from 1 504 samples and 140 were isolated by culture.The whole process just nee-ded 2.5 h.Conclusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the i-dentification of streptococcus pneumonia,but also provide evidence for antibiotic therapy.

OBJECTIVE To investigate the infection status and susceptibility of mycoplasma to 14 antibiotic agents. METHODS Mycoplasma was cultured and its susceptibility from 3577 patients with genitourinary tract infections were statistically analyzed. RESULTS The positive rate of mycoplasma was 43.9%.The positive rate of Ureaplasma urealyticum (Uu),Mycoplasma hominis (Mh ) and Uu+Mh were 33.4%,1.5% and 8.9%,respectively. The positive rate of Uu was significantly higher than that of Mh and Uu+Mh (P

Objective:To explore the assessment value of ultrasound images for the curative effect of placed umbrella-shaped filters in treating deep vein thrombosis in the lower limbs.Methods:A retrospective analysis was conducted on the clinical data of 85 patients with deep vein thrombosis of the lower extremities who admitted to Peking University Third Hospital Yanqing Hospital from October 2018 to October 2022.Based on the treatment effect and the improvement of clinical symptoms,the patients were divided into a cure group(38 cases),a significant effect group(27 cases)and an effective group(20 cases).All patients underwent the placement of an umbrella-shape filter and popliteal vein puncture on the affected side,and the urokinase was pumped into the body after a thrombolytic catheter was connected to micro pump.All patients underwent contrast-enhanced ultrasound examination,and the diluted ultrasound contrast agent was injected through a thrombolytic catheter on the 1st,3rd and 5th day after surgery,so as to analyze the perfusion situation of ultrasound contrast.Time intensity curve(TIC),time to peak(TTP),density peak intensity(DPI)and ascending branch slope(C)of the curve were obtained by using ultrasound contrast quantitative analysis software.And then,the quantitative parameters of patients among the 1st,3rd and 5th day after surgery were compared.Results:Compared with these before treatment,the reduction of the intima of the wall(82 cases),the better vascular compression(58 cases)and the clear echo in the lumen(61 cases)were significantly improved after treatment,and the differences were statistically significant(x2=52.407,61.917,74.518,P<0.05),respectively.The total effective rate was 100%.The TTP,DPI and C of the cure group,the significant effect group and the effective group were respectively(14.02±5.41)s,(-10.77±3.22)s and(4.91±1.33)dB/s on the 5th day after surgery.The TTP,DPI and C of the significant effect group[(17.95±7.41)s,(-13.87±4.01)s and(4.11±1.20)dB/s]on the 5th d after surgery were significantly different from those on the 1st day after surgery(cure group:t=11.615,-22.122,-16.112,P＜0.001,significant effect group:t=8.820,-13.073,-10.804,P＜0.001,effective group:t=6.791,-7.725,-6.633,P＜0.05),respectively.The DPI and C of the three groups on the 3rd day after surgery were significantly higher than those on the 1st d after surgery,while the TTP was significantly lower than that on the 1st day after surgery,and the differences were statistically significant(cure group:t=-18.857,-12.387,-7.254,-7.435,P＜0.001,effective group:t=-5.076,-6.919,P＜0.05),respectively.The DPI and C on the 5th d after surgery in the cure group and the significant effect group were significantly higher than those in the effective group(tDPI=12.219,7.457,tC=4.826,2.530,P＜0.05),respectively.Conclusion:The deep vein thrombosis of the lower extremities can choose the method of thrombolysis treatment after the umbrella-shape filter is placed,which can effectively intercept the thrombosis and can be indwelled in the vein in a long time,and which can also observe perfusion during inferior vena cava contrast,and which can also intuitive appear the recanalization of the veins after umbrella-shape filter is placed in patients with deep vein thrombosis of the lower extremities after the treatment of catheter thrombolysis.

Objective:To investigate the current situation of general practitioners' occupational pressure in Heilongjiang Province, and explore its relationship with professional identity and perceived organizational support. It aims to provide references for the construction of general practitioners and the development of general medical education.Methods:The general situation questionnaire, perceived organizational support scale, occupational pressure scale, and professional identity scale were applied through network and site surveys for collecting data. A total of 288 questionnaires were collected with 263 valid questionnaires (91.3%). Pearson correction analysis and bootstrap analysis were performed for data analysis.Results:The average score of occupational pressure among general practitioners was (115.95±22.40), and the results of Pearson correction analysis showed that perceived organizational support was negatively correlated with occupational pressure ( r=-0.413, P<0.01) and positive correlation with professional identity ( r=0.587, P<0.01). There were also significant negative correlations between professional identity and occupational pressure ( r=-0.442, P<0.01). Moreover, the mediating effect showed that professional identity had a certain mediating effect on the relationship between perceived organizational support and occupational pressure (LLCI=-0.2039, ULCI=-0.0760, P<0.05). Conclusion:General practitioners' occupational pressure was at a high level, which can be reduced through improving the perceived organizational support and professional identity.