Objective To study the optimum conditions of extraction and ? - cyclodextrin inclusion for the volatile oil from Radix Angelicae Sinensis, Rhizoma Chuanxiong and Cortex Cinnamomi in Shaofu Zhuyu Tablets. Methods The extraction conditions were evaluated by the ultimate oil extract ratio with single- factor analysis, and the inclusion technology were optimized by the inclusion output ratio and oil- bearing ratio in the inclusion with orthogonal test. Results The optimum conditions for extraction were as follows: smashing the medicinal material into coarse powder (through 10- mesh screen), adding 8 times of the water (mwater ∶ mpander=8 ∶ 1), extracting oil for 4 hours. The best conditions for inclusion were as: the ratio of Oil and ? - cyclodextrin being 1 mL ∶ 8g, and stirring for 2 hours at 50 ℃ . Conclusion The extraction and inclusion conditions in this study are optimal with high oil extract rate, reasonable inclusion process, and minimum cost.

Objective To establish a method for the determination of schizandrin, deoxyschizandrin and ?-schizandrin in Jiangtang soft capsule. Methods The sample was extracted by methanol. The chromatographic conditions were: a gradient mobile phase of methanol-water(65: 35)within 0～20 rain and methanol-water(70: 30)within 20～80 min, the wavelength at 250 nm. Results A linear range of schizandrin, deoxyschizandrin and ?-schizandrin was within 0.71 ?g～3.53 ?g(r=0.99998, n=5), 0.19?g～0.95 ?g(r=0.99993, n=5)and 0.36?g～1.80?g(r=0.99997, n=5) and the average recoveries were 100.44%, 98.06% and 101.14% respectively. Conclusion This method is easy, sensitive, specific and accurate for the determination of schizandrin, deoxyschizandrin and ?-schizandrin in Jiangtang soft capsule.

Objective To establish a quality control method for tanshinone ⅡA in Niaotong Tablets.Methods The content of tanshinone ⅡA was determined by TLC-scanning method. Benzene-ethyl acetate(19 ∶1) was used as the developer, ?s = 470nm,?R = 620nm,SX=3.Results A linearity was obtained from 0.44?g to 2.20 ?g of tanshinone ⅡA in Niaotong Tablets (r=0.9993,n=5);the average recovery rate was 98.08 %,RSD=3.29 %.Conclusion This method is simple, sensitive and reproducible for the determination of tanshinone ⅡA in Niaotong Tablets and Radix Salviae Miltiorrhizae.

Objective To establish a method for the determination of 5-O-methylvisamminol in Shufeng oral liquid.Methods The sample was extracted with methyl and HPLC method was used.The chromatographic conditions were as follows: Alltima-C18 column(150 mm ? 4.6 mm,5 ?m)with a mobile phase of methanol-water(41∶59),the detection wavelength at 293 nm and the flow rate being 1.0mL?min-1.Results A linearity was obtained from 0.12 ?g to 0.84 ?g of 5-O-Methyvisammioside in Shufeng oral liquid with a good correlation(r = 0.99998,n = 7).The average recovery was 99.8 % and RSD = 2.05 %(n = 5).Conclusion This method for determination of 5-O-Methyvisammioside in Shufeng oral liquid is simple,sensitive,specific and accurate.

AIM: To establish a RP-HPLC method for determining ephedrina hydrochloridum and pseudoephedrine hydrochloride in Chuanliting Spray(Herba Ephedrae,Folium Artenisiae argyi,Radix et Rhizoma Asari,etc.). METHODS: HPLC conditions were as follows: C_(18)(4.6 mm?250mm,5?m) column,0.2% phosphoric acid—acetonitrile(96∶4) as mobile phase with flow rate of 1mL/min,and the detection wavelength at 205nm.(RESULTS:) The calibration curve of ephedrina hydrochloridum was linear between 0.448-3.416?g(r=(0.999 8,) n=7).The average recovery was 100.26% and RSD=2.17%(n=5).The calibration curve of pseudoephedrine hydrochloride was linear between 0.16-1.12?g(r= 0.999 1,n=7).The average recovery was 101.60% and RSD =2.19%(n=5). CONCLUSION: The method is convenient and efficient,and can be used for quantitative analysis and quality control of this preparation.

Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 %ethanol. The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm?4.6 mm,5?m)with a mobile phase of acetonitrile-0.5 %glacial acetic acid,the detection wavelength being at 250 nm and the flow rate being 1.0 mL?min-1. Results A linearity was obtained from 0.338 ?g to 2.336 ?g of Puerarin in Huanglian Jiangtang Tablets with a good correlation (r=0.999997,n=7).The average recovery was 100.2% and RSD=1.80%(n=6). Conclusion This method for determination of Puerarin in Huanglian Jiangtang Tablets is easy,sensitive,specific and accurate.

Objective To establish a RP-HPLC method for the determination of gardenoside in Xinxue Tablets and to provide evidence for ameliorating its quality criterion.Methods HPLC conditions were as follows: C18(4.6?250 mm,5 ?m)column with temperature at 25 ℃,acetonitrile-water(15∶85) as mobile phase with flow rate of 1 mL/min,and the detection wavelength at 238 nm.Results Gardenoside was well separated from other components and had good linearity in the range of 0.128～1.024 ?g(r=0.999 2).The average recovery was 98.67 %and RSD=1.57 %.Conclusion The method is accurate,reproducible,and can be used for the quality control of Xinxue Tablets.

Objective To evaluate endoscopic management of bile duct benign and malignant stenosis after cholecystectomy.Methods A retrospective analysis was made on 120 bile duct benign and malignant stenosis developed after cholecystectomy, ERCP diagnosis and treatment was evaluated.Results Of the 120 cases, there was bile duct stenosis in 79 cases (including 61 cases with common bile duct stones), papillary inflamnatory stenosis in 15 cases, diverticulum associated duodenal papillitis in 8 cases, malignant biliary stricture in 8 cases.Among these 120 cases there were hilar benign stenosis in 3 cases, 3 cases with hilar malignant tumor, 4 cases of chronic pancreatitis.Benign bile duct stricture was treated with plastic stent drainage, concomitant calculi by endoscopic stone extraction.Papillary stenosis and papillitis were managed by EST or EPBD, inoperable malignant biliary stenosis by mental stent placement.Conclusions After cholecystectomy biliary stenosis treated by ERCP and endoscopic therapy is miniinvasive helping control biliary infection when the diagnosis of benign stenosis of bile duct is established.

AIM: To prepare and characterize a novel anti-human CD83 monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD83 transfectant (L929/CD83) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells. The hybridoma cells were screened with CD83 transfectant (L929/CD83 and 293/CD83) by FCM. The biological characteristics of antibody were investigated by rapid isotyping analysis, karyotype analysis, competitive inhibition test and Western blot. RESULTS: One hybridoma cell line named 9D8 was obtained, which had the property of secreting antihuman CD83 monoclonal antibody steadily, This mAb specifically recognized CD83 molecule expressed on human mature DC, activated T cells, and tumor cell line Daudi, myeloma cell line 8226. This mAb can recognize a distinct epitope from comercial mAb(HB15e). CONCLUSION: One hybndoma cell line has been developed successfully, which may lay a fundation for further study on the function of this molecule.

AIM: To prepare and characterize a mouse anti-human CD40 mutant monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD40 mutant transfectant (L929-CD40mu) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells. The hybridoma cells were screened with CD40 mutant transfectant (L929-CD40mu) by FCM. Faststrip analysis was performed to identify Ig subclass of this mAb. The epitope recognized by this mAb was detected by Bio-5C11 competitive assay. Western blot technique was adopted to identify the mAb. The proliferation of tumor cells in vitro was analyzed by MTT assay and apoptosis of tumor cells in vitro was analyzed by PI-annexin V assay. RESULTS: One hybridoma cell line named 10C5 was obtained, which had the property of secreting anti-human CD40 mutant monoclonal antibody continuously and steadily. This mAb specifically recognized human CD40 mutant molecule and induced the apoptosis of tumor cells in vitro. CONCLUSION: One hybridoma cell line which can secret a mouse anti-human CD40 mutant mAb has been prepared successfully. This mAb can inhibit the growth of tumor cells expressing CD40 mutant and induce their apoptosis in vitro.