1.Unique GGT --> GTT mutation at K-ras codon 12 in six human pancreatic cancer cell lines from Chinese patients.
Shuanzeng WEI ; Tonghua LIU ; Hongrui LIU ; Jie GAO
Chinese Medical Journal 2003;116(10):1585-1587
OBJECTIVETo investigate the K-ras mutation pattern in six pancreatic cancer cell lines from Chinese patients.
METHODSAll six cell lines were analyzed for mutation in exon 1 of the K-ras gene by polymerase chain reaction (PCR) and direct sequencing.
RESULTSAll 6 pancreatic cancer cell lines had GGT --> GTT mutation at K-ras codon 12 but no mutations at codon 13.
CONCLUSIONThe unique GGT --> GTT mutation at codon 12 plays a potential role in the carcinogenesis of pancreatic cancers in Chinese.
Adenocarcinoma ; genetics ; Asian Continental Ancestry Group ; Cell Line, Tumor ; China ; Codon ; Genes, ras ; genetics ; Humans ; Mutation ; Pancreatic Neoplasms ; genetics
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