1.Isolation and molecular characterization on Abbey Lake Orthobunyavirus (Bunyaviridae) in Xinjiang, China.
Ran LIU ; Guilin ZHANG ; Xiang SUN ; Zhong ZHENG ; Xiaoming LIU ; Yan ZHAO ; Shuankui LIU ; Rongli DANG ; Tongyan ZHAO
Chinese Journal of Epidemiology 2014;35(8):939-942
OBJECTIVETo monitor and discover medically important mosquito-borne viruses circulating in Xinjiang, China.
METHODSMosquitoes were collected from Abbey Lake wetland in Bortala, in Northern Xinjiang. Viral isolates were obtained through inoculating and serial passaging into susceptible mammalian host cells (BHK-21), identified by cytopathogenic effect (CPE) observation and plague forming assay. Genetic identification of viral isolates was conducted by RT-PCR, sequencing and phylogenetic analysis.
RESULTSA virus strain which causing CPE on BHK-21 cells, was isolated from the predominant Culex modestus (36.6%) and tentatively designated as Abbey Lake virus. Information on molecular identification revealed that Abbey Lake virus belonged to Orthobunyavirus genus within Bunyaviridae. Partial sequences (651 bp and 980 bp) of viral genomic S and M segment showed that Abbey Lake virus was phylogenetically related to Germiston virus that uniquely found in South Africa with 90.6% nucleotides and 95.0% amino acids similarities in S segment. However, viral M segment displayed much variability with 78.6% nucleotides and 86.1% amino acid similarities, suggesting a new member of Orthobunyavirus genus was discovered in the area.
CONCLUSIONIn this study, Abbey Lake virus was isolated and characterized indicating its potential circulation nature of this newly-emerged mosquito-borne virus.
Animals ; China ; Culicidae ; virology ; Orthobunyavirus ; classification ; genetics ; isolation & purification ; Phylogeny ; Sequence Analysis, RNA ; Viral Proteins ; genetics
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