Objective To investigate the change of sperm mitochondrial membrane potential in obese male infertility patients,and to explore the re?lationship of sperm mitochondrial membrane potential with the reactive oxygen species and free fatty acids in seminal plasma. Methods According to the research conditions,samples were randomly selected by cluster sampling from outpatient,and divided into normal body mass index fertile men as control group(n=51),normal body mass index infertile group(n=36),overweight infertile group(n=44),and obesity infertile group(n=45). Semen routine analysis was performed. Free fatty acid and reactive oxygen species in the seminal plasma was determined by ELSA method,sperm mitochondrial membrane potential was determined by flow cytometry. Results The rate of normal sperm mitochondrial membrane potential in nor?mal weight infertility(27.34%±13.38%),overweight infertility(28.26%±9.76%),obesity infertility group(25.27%±7.51%)were lower than the control group(35.12%±15.90%),the difference was statistically significant(P<0.05). Although obesity infertility group normal rate of mitochon?drial membrane potential were lower than normal weight infertility group and the overweight infertility group,but there was no statistically significant difference. The rate of sperm normal mitochondrial membrane potential was positively correlated with the rate of sperm progressive motility(r=0.29, P<0.01). Free fatty acid was positively correlated with reactive oxygen species in seminal plasma(r=0.30,P<0.01),reactive oxygen species in seminal plasma was negatively correlated with sperm normal mitochondrial membrane potential(r=-0.24,P<0.01). Conclusion Free fatty acid was elevated in the seminal plasma of overweight and obese patients with male infertility,which causes increased reactive oxygen species,reduced mitochondrial membrane potential,and eventually lead to the decline of sperm movement ability. Patients undergoing the treatment should be ad?vised to reduce the high fat diet and perform proper exercise.

Objective To summarize the effect of treatment for common bile duct stones by laparoscope combined with choledocho-scope, and to assess the feasibility in judgment the patency of common bile duct by stone basket. Methods To review and analyze the clini-cal data of 32 patients who were given choledocholithiasis operation through laparoscope combined with choledochoscope,and the operation method,effect and complications were compared. Results In this research,32 choledocholithiasis operations were carried out using laparo-scope combined with choledochoscope from January 2013 to January 2014. The stones of 31 patients were taken out completely,and there were no stone in the other patient. All 32 patients recovered without any complication. After operation,all 32 patients showed unrestricted inferior bile common duct and no residual stone by T-tube cholangiography. Conclusion It showed satisfactory effect in treatment of choledocholithi-asis by laparoscope combined with choledochoscope. Compared to traditional laparotomy,which showed less injure and faster recovery. It is a simple and feasible method to judge the patency of common bile duct using large size stone basket combined with methylene blue injection.

Objective To study the application of 99Tcm in rabbit cerebral thromboembolic stroke and thrombolysis effect of recombinant tissue plasminogen activator (rt-PA).Methods The 0.5 mL radioactive pertechnetate sodium (specification:5 mCi/2mL and radiation intensity 92.5 MBq/mL) was combined with 30 μL stannous chloride (5 mg/mL),and the 20 μL mixture was joined to whole blood,red blood cells,and plasma for labelling.Then 50 μL CaCl2 (0.5 mol/L) and bovine thrombin (50 IU/mL) were doped in mixture,and rapidly sucked into a polyethylene plastic pipe (PE80).Thrombus was formed for 2 h at 37 ℃ and cut into small pieces of 10 mm.Autologous blood clots combined with 99Tcm from external carotid artery were injected to internal carotid artery of rabbit,the radioactivity (counts per minute,CPM) was measured by gamma counting instrument,and the improvement of rt-PA 4.5 mg/kg (clinical equivalent dose) on this model was observed.Results After thromboembolism,CPM increased approximately by (5.1 ± 1.3) times,which suggested that the model was reliable.The rt-PA 4.5 mg/kg had significant progressive thrombolysis effect.Conclusion 99Tcm tracer technology could be applied to rabbit cerebral stroke model,which is stable and reliable

Objective To investigate the protective effects of Platycodon grandiflorum total saponins (PGTS) on acute lung injury (ALI) in rats and the related mechanisms.Methods Total of 60 SD rats were randomly (random number) divided into control group,model group,low-,middle-and high-dose PGTS group,and dexamethasone group,10 rats in each group.The latter 4 groups and dexamethasone group were injected with 50,100,200 mg/kg PGTS and 5 mg/kg dexamethasone,respectively.After 1 h,the latter 5 groups were intraperitoneally injected with mg/kg LPS to establish the ALI model.The clinical symptoms of the rats were observed.After 12 h,the arterial PaO2 and PaCO2,serum TNF-α and IL-10 level,lung wet/dry weight ratio (W/D),lung tissue SOD,GSH-Px and MDA level and NF-κB protein expression were determined.Results Rats in model group manifested noticeable symptoms of acute lung injury (ALI) and lung tissue lesions.In treatment group with appropriate PGTS dose,ALI symptoms and lung lesions were significantly alleviated,arterial PaO2 was markedly increased (P ＜ 0.05),PaCO2 was decreased obviously (P ＜ 0.05),serum TNF-α level was prominently decreased (P ＜ 0.05),IL-10 level was strikingly decreased (P ＜ 0.05),lung W/D ratio was significantly decreased (P ＜0.05),lung tissue SOD and GSH-Px level were distincdy increased (P ＜0.05),MDA was clearly decreased (P ＜ 0.05),and NF-κB protein expression was plainly decreased (P ＜ 0.05),compared with model group.Conclusions PGTS has undoubted protective effects on acute lung injury induced by LPS in rats.The mechanism may be associated with its role of anti-inflammation,anti-lipid peroxidation and down regulation of NF-κB protein level in lung tissue.

Unicentric Castleman disease (CD) is a rarely abnormal lymphoproliferative disease with unknown etiology and hyaline vascular variant is the most common histopathological type. The clinical manifestations of unicentric CD are solitary masses with slow growth, mainly occurring in the mediastinum, abdominal cavity, retroperitoneum, pelvis, and neck. Application of multidisciplinary cooperation between surgical, radiologic, pathological and oncological experts is necessary for the diagnosis and treatment of unicentric CD, in order to achieve good treatment results. The best treatment method for unicentric CD is surgical resection. With the accumulation of experience and technological progress, there may be more precise and minimally invasive surgical methods such as laparoscopic or robotic techniques besides open surgery. The authors report the diagnosis and treatment of unicentric hepatic CD, aiming to provide reference for related treatments.

Objective To explore the changes of microvasculature in the macular area before and after the surgery of acute primary angle-closure glaucoma(APACG)complicated by cataract by using optical coherence tomography angiogra-phy(OCTA).Methods In this retrospective study,23 patients(46 eyes)with APACG(one eye in acute attack and the other eye in preclinical stage)complicated by age-related cataract in both eyes admitted to our hospital from May to No-vember 2023 were selected as the research subjects.The 23 eyes in the acute attack were taken as the observation group,and the 23 contralateral eyes in the preclinical stage were taken as the control group.After the intraocular pressure was sta-bilized,eyes in the observation group underwent phacoemulsification with intraocular lens implantation and goniosynechial-ysis;two weeks later,eyes in the control group underwent phacoemulsification with intraocular lens implantation.The pa-rameters of the foveal avascular zone(FAZ),parafoveal and perifoveal retinal vessel density,and foveal choroidal capillary perfusion area were examined by OCTA before and after surgery.The results were analyzed statistically.Results Before the surgery,the uncorrected visual acuity in the observation group was lower than that in the control group,and the in-traocular pressure was higher than that in the control group(both P＜0.01).At1 week,1 month and 3 months after sur-gery,the uncorrected visual acuity was higher and the intraocular pressure was lower than that before surgery in the two groups(all P＜0.05).The FAZ area and perimeter in the observation group were bigger,but the FAZ circularity index was smaller than that in the control group before the surgery(all P＜0.05).At 1 week after the surgery,the FAZ perimeter in the observation group was bigger,and the FAZ circularity index in the observation group was smaller than that in the con-trol group(both P＜0.05).After the surgery,the FAZ area and perimeter of affected eyes in both groups showed a down-ward trend,while the FAZ circularity index showed an upward trend.Compared with the values before surgery,the FAZ area and perimeter decreased,but the FAZ circularity index increased in the two groups 3 months after the surgery(all P＜0.01).The parafoveal and perifoveal retinal vessel densities in the observation group were lower than those in the control group before and at different time points after the surgery(all P＜0.05).The foveal choroidal capillary perfusion area in the observation group was lower than that in the control group before and at different time points after the surgery(all P＜0.01).The foveal choroidal capillary perfusion area in both groups after surgery increased than that before surgery;3 months after the surgery,the foveal choroidal capillary perfusion area was higher than that before the surgery in the obser-vation group(P＜0.01),but there was no significant difference in that before and after surgery in the control group(P＞0.05).Conclusion The FAZ area and perimeter are bigger,the FAZ circularity index is smaller,and retinal vessel den-sity of the macular area and foveal choroidal capillary perfusion area are lower in the acute attack eyes than those of the preclinical eyes with APACG complicated by age-related cataract.Phacoemulsification with intraocular lens implantation and goniosynechialysis is safe and effective in the treatment of acute attack APACG eyes with cataracts.After the surgery,the FAZ area and perimeter will decrease,while the FAZ circularity index and the foveal choroidal capillary perfusion area will increase.OCTA provides a reference for a more objective evaluation of the changes in microvasculature in the macular area of APACG patients before and after surgery.