Objective To observe the effect of intrathecal p38 MAPK inhibitor(SB203580) treatment on neuropathic pain and the expression of p38 MAPK and BDNF in dorsal horn of spinal cord in rats with chronic constriction injury(CCI),So as to investigate the possible mechanisms of neuropathic pain.Methods Totally 30 SD rats were evenly randomized into 3 groups(n=10) :sham group receiving intrathecal injection of sodium chloride,control group receiving intrathecal injection of sodium chloride and CCI surgery,and SB203580 group receiving intrathecal injection of SB203580 and CCI surgery.SB203580(0.1 ml/kg) was administered 0.5 h before and 1-14 d after CCI surgery.The mechanical thresholds were tested 24 h before and 4-14 d after CCI surgery.p38 MAPK expression and BDNF release in the dorsal horn were determined using immunohistochemistry method 14 d after CCI surgery.Results The mechanical thresholds in the control and SB203580 groups were significantly lower after CCI surgery compared with that before CCI surgery(P0.05).Compared with the sham operation group,the mechanical thresholds were significantly lower in the other two groups after CCI surgery(P

Objective To investigate the effect of propofol pretreatment on hypoxia-induced apoptosis of alveolar epithelial type Ⅱ (AE Ⅱ) cells in fetal rats. Methods Primary cultured AE Ⅱ cells isolated from fetal rats were seeded in 96-well plates (1 × 106/L, 180 μl/well) and randomly assigned to one of 3 groups (n = 72each):normal control group (group C), hypoxia group (group H) and propofol-hypoxia group (group P-H).Group H and P-H were exposed to hypoxia (5% O2). In group P-H, propofol (final concentration 5 μ mol/L) was added 1 h prior to hypoxia (5% O2). The apoptotic rate and expression of hypoxia-inducible factor (HIF)-1αmRNA, Bnip3L mRNA, HIF-1α protein and Bnip3L protein were determined at 3, 12, 24 and 48 h of hypoxia.Results The apoptotic rate and expression of HIF-1α mRNA, Bnip3L mRNA, HIF-lα protein and Bnip3L protein were significantly up-regulated in group H compared with group C (P ＜ 0.05). Propofol pretreatment could significantly inhibit the hypoxia-induced changes mentioned above (P ＜ 0.05). Conclusion Propofol pretreatment can inhibit hypoxia-induced apoptosis of AE Ⅱ cells, and the mechanism is related to inhibition of HIF-1αactivation and down-regulation of Bnip3L expression in fetal rats.

Objective:To explore the clinical effects of enhanced recovery after surgery (ERAS) nursing concept in perioperative period of patients with gynecological malignant tumors undergoing minimally invasive surgery.Methods:A total of 60 patients with gynecological malignant tumors who underwent minimally invasive surgery in Shanghai First Maternity and Infant Hospital from August 2018 to May 2019 were selected by convenience sampling method. According to the random number table, they were divided into the non-ERAS group and ERAS group, with 30 cases in each group. Patients in the non-ERAS group were given routine nursing while patients in ERAS group were given enhanced recovery after surgery nursing. The first feeding time after operation, the out of bed activity time on 3 days after operation, activity amount of 3 days after operation, anal exsufflation time, defecation time, length of hospital stay, total hospitalization expenditures, complications, pain score and satisfaction score were compared between the two groups.Results:The out of bed activity time on 3 days after operation and activity amount of 3 days after operation of patients in ERAS group were both more than those of the non-ERAS group, volume of fluid input on 3 days after operation was less than that of the non-ERAS group, anal exsufflation time and defecation time were earlier than those of the non-ERAS group, the pain score on the second day after the operation was better than that of the non-ERAS group and satisfaction degree was higher than that of the non-ERAS group. The differences were statistically significant ( P<0.05) . Conclusions:Application of enhanced recovery after surgery nursing in minimally invasive surgery for gynecologic malignant tumors can improve medical experience of patients, accelerate the postoperative recovery process, shorten the recovery time of patients and help patients to recover, which is worthy of promotion and application.