1.Research on biological and genetic characteristics of human placenta mesenchymal stem cells cultured in vitro.
Dongming ZHENG ; ; Xiaorui LI ; Yue LIU ; Haiyan LI ; Ming MA ; Yin DENG ; Jianglin LI ; Shuangqing CEN ; Rong ZHANG ; Quan HAI
Chinese Journal of Medical Genetics 2016;33(4):471-475
OBJECTIVETo investigate the biological characteristics and genetic features of human placenta mesenchymal stem cells (hPA-MSCs) cultured in vitro in order to assess its safety for clinical use.
METHODSThe shapes of the 1st, 3rd, 5th, 7th, 10th, 13th, 17th and 20th generation hPA-MSCs cultured in vitro using serum-free culture medium were observed. Their cell cycle, cell surface markers, and karyotype were analyzed, and relevant genes and cytokines were measured.
RESULTSThe shape of hPA-MSCs has remained as fusiform or short fusiform, and there was no significant change. About 93% of hPA-MSCs cells were in G0/G1 phase and remained stable. No obvious chromosomal translocation, loss or inversion was noted by karyotyping analysis. Cytokines expression level remained stable. Related gene expression level as a whole was on the decline, but the gene expression level of the first five generations showed very slight variations, with genetic characteristics remaining stable.
CONCLUSIONThe hPA-MSCs cultured in vitro with serum-free medium has retained stable in the first five generations.
Cells, Cultured ; Cytokines ; analysis ; Female ; Humans ; Karyotyping ; Mesenchymal Stromal Cells ; physiology ; Placenta ; cytology ; Pregnancy
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