0.05),but that MFI and/or PPC of CAR in the 2 types cells markedly increased compared with lymphocytes in the same group(Pa

Objective To investigate influence of nano-?-linolenic acid on expression of macrophage migration inhibitory factor (MIF) in murine model with viral myocarditis (VM).Methods Eighty male Balb/c mice were randomly divided into 4 groups equally:control group,model group,low and high dose intervention group.Mice in control group were inoculated introperitoneally with Eagle's solution.Every mouse in other groups was treated with 0.1 mL Coxsackie B3 virus(CVB3) intraperitoneally.Then,mice in low and high dose intervention group were treated with 60 and 180 mg/kg nano-?-linolenic acid solution for 1 week,respectively.Mice in control group and model group were treated with 9 g/L saline for 1 week.All mice were killed on day 15.The expression levels of myocardial MIF mRNA and protein were detected by reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry.Serum MIF concentration were exa-mined by enzymelinked immunosorbent assay(ELISA).Myocardial histopathology was determined with hematoxylin and eosin stain.Results The mortality rate was 0,45%,30% and 20% in control group,model group,low and high dose intervention group,respectively.The mortality rate was significantly lower in high dose intervention group compared with model group (P

OBJECTIVETo observe the effect of Astragalus Injection (ASI) combined with rat's mesenchymal stem cells transplantation (rMSCs) for repairing spinal cord injury in rats.

METHODSOne hundred and twenty Wistar rats were randomly divided into 6 groups, named respectively by letters from A-F. they were treated respectively with none, PBS solution, ASI, rMSCs, and ASI + rMSCs, with the ASI administered via intraperitoneal injection and the rMSCs given by local injection to the spinal cord, on the 3rd day of operation. The condition of nerve function recovery was assessed on the 7th, 14th, 21st and 28th day after treatment by scoring according to the Basso-Beattie-Bresnahan (BBB) locomotor rating scale. Besides, the pathological change of the injured spinal cord was observed and expressions of glial fibrillary acidic protein (GFAP) and neurofilament-M (NF-M) in the BrdU-labeled rMSCs in the spinal cord tissue were examined by immune histochemistry.

RESULTSThe recovery of spinal cord in Group D, E and F was better than that in Group B and C, showing higher BBB scores. As compared with Group E, Group F showed a higher score of nerve function and a milder inflammatory cell infiltration with lessened tissue edema in the spinal cord and more active proliferation of gliacyte. Double-labelled immunohistochemical examination showed that the transplanted rMSCs were alive in the host's spinal cord, revealing the expressions of GFAP and NF-M from the 7th day after transplantation, which were migrating to the injured site. The amount of GFAP and NF-M positive cells in Group F was much more than that in Group E (P < 0.05)

CONCLUSIONTransplantation of rMSCs is an effective method in treatment of the spinal cord injury; ASI has the capacity for inducing rMSCs differentiated into neurons, and could synergize with rMSCs to promote the repairing of the spinal cord from injury.

Animals ; Astragalus Plant ; Drugs, Chinese Herbal ; therapeutic use ; Male ; Mesenchymal Stem Cell Transplantation ; Nerve Regeneration ; Phytotherapy ; Rats ; Rats, Wistar ; Spinal Cord Injuries ; therapy

0.05).Conclusions It suggests that immune dysfunction exists in child patients with VM,MIF,TNF-? and IL-1? may play a role in the VM pathogenesis,and they can be a new indicator for VM.

MicroRNA (miRNA), small non-coding RNA consisted of 19-24 nucleotides, are able to regulate gene expression at the post-transcriptional level. The aberrant expressions of miRNA have been found in various cancers and contribute to carcinogenesis by promoting the expression of proto-oncogenes or by inhibiting the expression of tumor suppressor genes. miRNA are related closely with the oncogenesis, progression, and prognosis of tumors. The discovery of the aberrant expression of miRNA in pancreatic ductal adenocarcinoma (PDA) and its target genes are helpful for the understanding of the pathogenesis of PDA and for the early diagnosis and prediction of this disease. In this paper, we summarize the recent research advances in miRNA expression in PDA and its target genes and discuss the potential role of miRNA in the diagnosis, and treatment of PDA.
Carcinoma, Pancreatic Ductal ; genetics ; Humans ; MicroRNAs ; genetics ; Pancreatic Neoplasms ; genetics

OBJECTIVETo study the relationship between fetomaternal cellular traffic and hepatitis B virus (HBV) intrauterine infection.

METHODSMaternal DNA and fetal DNA were amplified by short tandem repeat (STR)-polymerase chain reaction (PCR), allele-specific PCR (As-PCR) and heminested PCR (hemi-nPCR). Cell transfer from mother-to-fetus or fetus-to-mother was determined by detecting the existence of TH01, GSTM1 and ACE. The relationship between cell transfer from mother-to-fetus and HBV intrauterine infection was analyzed by nested case-control study.

RESULTS26 of the 42 informative mother-baby pairs indicated mother-to-fetus cell traffic, 32 of the 40 informative mother-baby pairs indicated fetus-to-mother cell traffic and two-way cell traffic occurred in 10 mother-baby pairs. Statistical analysis demonstrated that the mother-to-fetus instead of fetus-to-mother cell traffic presented the association with HBV intrauterine infection. There was no significant correlation between mother-to-fetus cell traffic or the fetus-to-mother cell traffic. Both mother-to-fetus cell traffic and PBMC HBV DNA positivity appeared in pregnant women were risk factors of HBV intrauterine infection but the two did not manifest the interaction. The positive risk factors of positivity PBMC HBV DNA in newborns would included mother-to-fetus cell traffic and PBMC HBV DNA in pregnant women, also did not display the interaction.

CONCLUSIONThe cell traffic from HBsAg positive mother to fetus had more contribution to HBV intrauterine infection, which was possibly one of the HBV routes of intrauterine infecting.

Biological Transport ; Cell Movement ; physiology ; Child ; Child, Preschool ; DNA, Viral ; blood ; Female ; Fetal Blood ; cytology ; Hepatitis B ; transmission ; Humans ; Infant ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; Leukocytes, Mononuclear ; pathology ; Maternal-Fetal Exchange ; Pregnancy ; Pregnancy Complications, Infectious ; Protein Transport ; physiology

Clinical study of modified Ganmai Dazao decoction in the treatment of yang deficiency climacteric depression and observe the effects of modified Ganmai Dazao decoction on neuroendocrine system in patients with yang deficiency climacteric depression. 86 cases were randomly divided into treatment group treated with modified Ganmai Dazao decoction and control group treated with Deanxit. The curative effect was evaluated with Hamilton's depressive scale (HAMD) and pittsburgh sleep quality scale (PSQI) before and at the end of the two and four weeks of the treatment, the serum levels of serotonin (5-HT), norepinephrine (NE), estradiol (E2), follicle stimulating hormone (FSH), luteotropic hormone (LH) were detected before and after the four weeks of the treatment The results showed that the total effective power of treatment group was 88.4% and the total effective power of control group was 81.4% after four weeks interference, with insignificant difference between the two groups. After two and four weeks of the treatment, the score of HAMD decreased remarkably in both groups (P < 0.01), with insignificant difference between the two groups in same phase. After two and four weeks of the treatment, the total score of PSQI decreased remarkably in both groups (P < 0.05), with significant difference between the two groups after four weeks (P < 0.01). After four weeks of treatment, the serum levels of 5-HT and NE increased (P < 0.01), with insignificant difference between the groups. After four weeks of treatment, the serum levels of E2 increased obviously (P < 0.05), the levels of FSH decreased obviously (P < 0.05), the levels of LH decreased insignificant, with insignificant difference between two groups. This study indicates that modified Ganmai Dazao decoction has obvious therapeutic effects in the treatment of climacteric depression, and showed equivalent efficacy with Deanxit, and modified Ganmai Dazao decoction has better effect on improving the sleep quality in patients than Deanxit, the effect of improved clinical symptoms may be through adjusted levels of 5-HT, NE, E2, FSH and LH of climacteric depression.
Adult ; Depression ; blood ; drug therapy ; etiology ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Follicle Stimulating Hormone ; blood ; Humans ; Menopause ; drug effects ; psychology ; Middle Aged ; Neurosecretory Systems ; drug effects ; metabolism ; Norepinephrine ; blood ; Phytotherapy ; Serotonin ; blood

Objective To investigate renal tissue changes in rats exposed to 16 Hz/130 dB infrasound.Methods Twenty male Sprague-Dawley adult rats were randomly divided into a control group,a pseudo-infrasound group,and two infrasound groups-A and B.Groups A and B were exposed to 16 Hz/130 dB infrasound for 2 h or4 h daily respectively over 7 days.Pathological and uhrastruetural changes in their renal tissues were observed with a light microscope and an electron microscope.Results Under the light microscope,Bowman's capsules expanded,epithelial cells were shed,and a little secretion was found in the renal tubules of infrasound group A.Slight degeneration and necrosis of the renal tubules and glomerular exudation could be observed in group B.Under the electron microscope,a large number of lysosomes displayed hyperplasia,there was interstitial edema,and leukocyte pavementing wag found in group B.Other changes such as swelling of podocytes' processes,fusion of foot processes,and vacuolization in the mitochondria could be observed in both infrasound groups.Conclusion Lengthy exposure to 16 Hz/130 dB infrasound can cause severe pathological and uhrastruetural changes in renal tissue,at least in rats.

OBJECTIVETo investigate the frequency and type of PHEX gene mutations in children with X-linked hypophosphatemic rickets (XLH), the possible presence of mutational hot spots, and the relationship between genotype and clinical phenotype.

METHODSClinical data of 10 children with XLH was retrospectively reviewed. The relationship between gene mutation type and severity of XLH was evaluated.

RESULTSPHEX gene mutations were detected in all 10 children with XLH, including 6 cases of missense mutation, 2 cases of splice site mutation, 1 case of frameshift mutation, and 1 case of nonsense mutation. Two new mutations, c.2048T>C and IVS14+1delAG, were found. The type of PHEX gene mutation was not associated with the degree of short stature and leg deformity (P=0.571 and 0.467), and the mutation site was also not associated with the degree of short stature and leg deformity (P=0.400 and 1.000).

CONCLUSIONSMissense mutation is the most common type of PHEX gene mutation in children with XLH, and c.2048T>C and IVS14+1delAG are two new PHEX gene mutations. The type and site of PHEX gene mutation are not associated with the severity of XLH.

Adolescent ; Child ; Child, Preschool ; Familial Hypophosphatemic Rickets ; genetics ; Female ; Humans ; Infant ; Male ; Mutation ; PHEX Phosphate Regulating Neutral Endopeptidase ; genetics ; Retrospective Studies