Objective To investigate the application of multi-detector row spiral CT(MDCT)and multi-planer reconstruction(MPR)in identify the anatomy detail of normal adult groin region.Methods We retrospectively collected the CT images of 50 adult subjects with normal groin anatomic structure underwent groin region thin-slice MDCT scans between July and December 2009,30 males and 20 females,obtained the coronal and sagittal views by MPR,investigated the value of different plans in identifying anatomic detail.Results Bilateral inferior epigastric artery(100/100,100%),spermatic cord(60/60,100%),and round ligament of uterus(40/40,100%)were well identified on all plans in all subjects.The bilateral "radiological femoral triangle" could be demonstrated on coronal views in all subjects(100/100,100%).The bilateral inguinal ligament were visible on coronal view in all subjects(100/100,100%)and on sagittal views in 34 subjects(68/100,68%),but on axial views was identified in 3 male subjects(6/100,6%).The bilateral inguinal canal and deep inguinal ring were reliably visible on coronal views in all subjects(100/100,100%),and on sagittal views in 46 subjects(92/100,92%).On coronal views,the widths of inguinal canal was(0.97?0.35)cm in left,(0.89?0.23)cm in right for males,and(0.62?0.11)cm in left,(0.71?0.11)cm in right for females.No significant difference was found between two sides(P=0.059 in males,P=0.067 in females),but there were significant differences between males and females(P=0.007 in left,P=0.009 in right).Transverse diameter of deep inguinal ring was(1.32?0.31)cm in left,(1.31?0.36)cm in right for males,and(1.07?0.35)cm in left,(1.07?0.30)cm in right for females.No significant difference was found between two sides(P=0.344 in males,P=0.638 in females),but there were significant differences between males and females(P=0.001 in left,P=0.002 in right).Conclusion MDCT with different plans plays an important role in identify the anatomic details of groin region,the coronal views especially.

Objective:To explore the feasibility of mesencephalon progenitor cells as dopamine neuron's graft in treatment of Parkinson disease(PD) in rats. Methods: Animal model of PD were established in SD rats by 6 hydroxydopamine. Mesencephalon progenitor cells were obtained from 13 day old fetal rats. After in vitro proliferation and differentiation, the cells were planted into PD rat striatum. Results: Progenitor cells were expanded in an undifferentiated state with endothelia growth factor and basic fibroblast growth factor as mitogens. IL 1 and striatal culture media further increased the number of TH immunoreactive cells. Transplantation of these neurons relieved the rotation symptom of PD rats. Conclusion: Transplantation of mesencephalon progenitor cells has certain effects on PD rats.

The sequences of ITS, matK, rbcL and psbA-trnH of 9 Gynostemma species or variety including 38 samples were compared and analyzed by molecular phylogeny method. Hemsleya macrosperma was designated as outgroup. The MP and NJ phylogenetic tree of Gynostemma was built based on ITS sequence, the results of PAUP phylogenetic analysis showed the following results: (1) The eight individuals of G. pentaphyllum var. pentaphyllum were not supported as monophyletic in the strict consensus trees and NJ trees. (2) It is suspected whether G. longipes and G. laxum should be classified as the independent species. (3)The classification of subgenus units of Gynostemma plants is supported.
Gynostemma ; classification ; genetics ; Molecular Sequence Data ; Phylogeny ; Plant Proteins ; genetics ; Sequence Analysis, DNA

AIM and METHODS: To study the culture met ho d of human neural stem/progenitor cells, these cells derived from human fetal fo rebrains were maintained and expanded in serum-free defined medium containing ba sic fibroblast growth factors (bFGF), epidermal growth factor (EGF) and B27. Whe n they formed neurosphere, these three factors and supplemented FBS were removed to induce differentiation. Cell were cultured for 12-14 d, then fixed for immun ocytochemistry examination. RESULTS: This period of expansion resulted in a 107-fold incre ase in this heterogeneous population of cells. Upon differentiation, they form n eurons, astrocytes and oligodendrocytes, the three main phenotypes in the CNS. CONCLUSION: These results demonstrate the feasibility of long-t erm in vitro expansion of human neural progenitor cells. The advantages of s uch a population of neural precursors for allogeneic transplantation, including t he ability to provide an expandable, well-characterized, defined cell source, ca n form specific neuronal or glial subtypes.

Objective To explore the expression of chemerin and chemerin receptor ( chemokine-like receptor 1, CMKLR1) during different periods of non-alcoholic fatty liver disease ( NAFLD) rat model induced by methionine- and choline-deficient ( MCD) diet. Methods Thirty-six Wistar rats were divided into control group and MCD group in random. After one week quarantine and acclimation period, these two groups were fed either normal chow or MCD diet. The animals were respectively sacrificed at the first week, the forth week, and the tenth week. The levels of alanine transaminase (ALT), blood lipid profile, liver function, and the content of triglyceride in liver were detected. HE staining was done to observe the morphologic change of liver. The mRNA expression changes of chemerin and CMKLR1 in liver were measured using real-time PCR, and the change in chemerin mRNA level was further confirmed in liver by Northern blot. Finally, the concentration of chemerin in serum was measured by Western blot. Results The mRNA level of chemerin decreased significantly after four and ten weeks MCD feeding, although no obvious changes were found at first week, similar changes were found in serum chemerin (1.00±0.11 vs 0.96±0.39; 1.00±0.12 vs 0.21 ±0.77; 1.00±0.42 vs 0.21 ±0. 11). Contrasting with the change of chemerin(1.00±0.08 vs 0.72±0.10;1.00±0.24 vs 0.63±0. 31 ;1.00±0.05 vs 0.50±0.13), the mRNA level of CMKLR1 increased after MCD feeding( 1.00±0. 14 vs 0. 84±0. 26; 1.00±0. 38 vs 1. 51 ±0. 33; 1. 01 ±0. 13 vs 1. 84 ± 0. 39 ). Conclusion The change of chemerin and its receptor may participate in the process of the nonalcoholic fatty liver disease.

Objective To investigate the cognition and compliance with the rehabilitation of patients after ACL reconstruction, explore main problem on health education, and provide theoretical basis. Methods 142 patients needing operation were collected from December 2009 to April 2010 in our hospital with serf-design questionnaire about cognition and compliance after ACL. The data were analyzed by SPSS 17.0 software. Results The compliance was associated with patients' age. There were significant correlation between compliance and factors of doing rehabilitation in hospital. The age was influencing factors of early functional exercise (P＜0.05). Conclusions We should increase the level of the health education, improve the patients' cognition, and pay more attention to patients'compliance to help the injured person return to a high level of functional ability.

Objective To observe the effect of non-surgical periodontal therapy on levels of lipoprotein-associated phospholipase A2 (LP-PLA2) and lipid profile markers in chronic periodontitis (CP)subjects with hyperlipidemia.Methods Forty CP subjects with hyperlipidemia (mean age 56.1 ± 10.3 years old,male 20,female 20) were enrolled.In addition to periodontal clinical parameters,serum concentrations of LP-PLA2,serum lipid profiles including total cholesterol (TC),triglyceride (TG),high-density lipoprotein(HDL),low-density lipoprotein (LDL),and white blood cell (WBC) counts were determined at baseline and 3 months after non-surgical periodontal therapy.Results After non-surgical periodontal therapy,all clinical parameters improved significantly [probing depth (PD):from 3.93 (3.63,4.48) mm to 2.47 (2.06,2.92) mm; clinical attachment loss:from 5.14(4.51,5.46) mm to 4.03 (3.38,4.33) mm; plaque index from 1.28 (0.94,1.61) to 0.77 (0.65,1,13) ; bleeding on probing percentage from 99.82％ (86.25％,100.00％) to 33.49％ (29.45％,43.30％) ; P ＜ 0.001.Levels of LP-PLA2,TC,TG and WBC counts were reduced significantly [LP-PLA2:from (30.06 ± 9.96) mg/L to (23.80 ± 14.76) mg/L,P =0.019; TC:from 5.57(5.26,6.25) mmol/L to 5.36(5.16,5.86) mmol/L,P =0.001 ; TG:from 1.79 (1.55,2.01) mmol/L to 1.57 (1.34,1.79) mmol/L,P ＜ 0.001 ; WBC counts:from 5.90(5.40,6.55) × 103 cell/L to 5.45(5.13,5.80) × 103 cell/L,P ＜0.001] ; HDL level increased significantly [from 1.12 (0.97,1.49) mmol/L to 1.23 (1.13,1.53) mmol/L,P =0.001] ; the reduction of LP-PLA2 (△LP-PLA2) was correlated with the reduction of PD (△PD) (r =0.367,P =0.020).Conclusions The results indicate that non-surgical treatment reduces systemic LP-PLA2 and WBC levels and improves lipid profiles.These results suggest that non-surgical periodontal therapy may reduce the risk of cardiovascular disease in some subjects with hyperlipidemia.

Objective To investigate the effects of bradykinin (BK) on the proliferation of rabbit corneal endothehal ceils (RCECs) and the expression of tight junction-related proteins zonula occludens-1 (ZO-1) and zonula occludens-1-associated nucleic-acid-binding protein (ZONAB),and to explore the underlying mechanisms of BK on cell proliferation in corneal endothelium.Methods RCECs at logarithmic growth phase were treated with different concentrations of BK (0.01,0.1,1,10 μmol · L-1) BK group,with the controls left untreated.Morphological changes of cells in each group were examined under phase-contrast microscope,and MTT assays were used to detect cell proliferation at 24 h,48 h,72 h,96 h after BK treatment.And,at 72 h,the expression levels of ZO-1 and ZONAB protein were determined by Western blot.Results After 72 h of treatment,the cells in each group were fused into pieces and closely linked into a monolayer;but after 96 h,the growth of the cells was restricted,with the intercellular space become larger and the cells exfoliated.Compared with the control group,BK induced a significant increase of absorbance value and cell viability,and the differences were statistically significant (all P ＜ 0.001),and the promoting effects showed a concentration-dependent manner,and 1 μmol · L-1 BK demonstrated the strongest regulative effect (P ＜ 0.001).Western blot results showed that BK upregulated the expression of ZO-1 and ZONAB protein in a concentration-dependent manner.Conclusion BK can stimulate the proliferation of RCECs in a time-and concentration-dependent manner,and the mechanisms are probably associated with ZO-1/ZONAB-mediated signaling pathway.

The National Committee for Quality Assurance(NCQA)and the Pharmacy Quality Alliance(PQA)used the American Geriatrics Society(AGS)Beers Criteria to establish the quality measure system of high-risk medications and potentially harmful drug-disease interactions in the elderly.Medications included may be harmful to elderly adults,and negatively affect health care plans' quality ratings.AGS experts conducted a comprehensive literature review and prepared a list of drug-therapy alternatives with supporting references.NCQA,PQA,the 2015 AGS Beers Criteria panel,and the Executive Committee of the AGS reviewed the drug therapy alternatives and nonpharmacological approaches.Prescribers,pharmacists,patients,and health care plans may benefit from this list.

Elderly individuals suffer from a variety of underlying diseaes as a result of physiological decline and are prone to adverse drug reactions and drug-induced diseases.Polypharmacy is a form of inappropriate medications and may increase the risk of adverse drug events,triggering prescription cascades and resulting in poor prognosis for the elderly.Identification and early intervention of inappropriate medications is critical in reducing the risk of medication complications and preventing adverse drug reactions.In recent years,research efforts have focused on the development of criteria for inappropriate medications in the elderly,the use of anticholinergic and sedative agents,polypharmacy issues and deprescribing of medications,among others.Research on inappropriate medications in elder people in China has been inadequate.