Objective To observe the inhibitory role of valproic acid (VPA) in hypertrophic cardiomycytes induced by angiotensin Ⅱ (Ang Ⅱ)in rats. Methods The cultured rat cardiomyocytes were divided randomly into control group, hypertrophic group and VPA group. They were treated with Ang Ⅱ to induce hypertrophy and then given with VPA. The morphologic changes of cardiomyocytes were observed under the contrast phase microscope and electron microscope. The mRNA levels ofβ-MHC were exmained by reverse transcriptase polymerase chain reaction (RT-PCR) method. The protein expression of C-FOS was exmanined by immunohistochemistry method. Results Stimulated by Ang Ⅱ, the cardiomyocytes were enlarged under the contrast phase microscope and the ultrastructure also changed. After stimulated by Ang Ⅱ, the mRNA level of β-MHC and the protein expression of C-FOS increased in the hypertrophic cardiocytes. Given by VPA, these data decreased accordingly. Conclusion VPA may inhibit the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in some degree.

Objective To observe the inhibitory role of valproic acid(VPA) in hypertrophic cardiomycytes induced by angiotensin Ⅱ(AngⅡ)in rats.Methods The cultured rat cardiomyocytes were divided randomly into control group,hypertrophic group and VPA group.They were treated with AngⅡ to induce hypertrophy and then given with VPA.The morphologic changes of cardiomyocytes were observed under the contrast phase microscope and electron microscope.The mRNA levels of ?-MHC were exmained by reverse transcriptase polymerase chain reaction(RT-PCR) method.The protein expression of C-FOS was exmanined by immunohistochemistry method.ResultsStimulated by AngⅡ,the cardiomyocytes were enlarged under the contrast phase microscope and the ultrastructure also changed.After stimulated by AngⅡ,the mRNA level of ?-MHC and the protein expression of C-FOS increased in the hypertrophic cardiocytes.Given by VPA,these data decreased accordingly.Conclusion VPA may inhibit the cardiomyocyte hypertrophy induced by angiotensin Ⅱ in some degree.

ObjectiveTo discuss the relationship between patella location and chondromalacia patella,explore its mechanism and clinical significance.Methods Knee joint MRI was performed in 1052 patients (506 men and 546 female).Among them,there were 299 patients( 100 men and 199 female)with chondromalacia patella.They were divide into the group of 1-19,the group of 20-39,the group of 40-59 years old and the group of older than 60 years to compute the positive rate respectively.Insall-Salvati method was used to measure the length of chondromalacia ligaments(L) and the length of chondromalacia path(P).The relationship between patella location and chondromalacia was tested by using t test and x2 test.ResultsThe total positive rate of chondromalacia patella was 28.4％ (299/1052).In female it was 36.4％ (199/546) and in men was 19.8％ (100/506).The group of 1-19 years old had 16 patients ( 16.8％,16/95).The group of 20-39 years old had 71 patients( 17.9％,71/396).The group of 40-59 years old had 116 patients ( 33.2％,116/349 ).The group of older than 60 years had 96 patients (45.3％,96/212).The positive rate of chondromalacia patella increased with age.The L/P value of normal group and chondromalacia patella group were 1.15 ±0.15 and 1.24 ±0.17 respectively.The L/P value of normal group of men and women were 1.13 ± 0.15 and 1.17 ± 0.14 respectively.The L/P value of chondromalacia patella group of men and women were 1.20 ±0.17 and 1.26 ±0.16 respectively.The InsallSalvati index of age groups showed significant differences.The L/P value of pathological changes group and normal group of 1-19 years old were 1.38 ± 0.25 and 1.24 ± 0.16 respectively.The L/P value of pathological changes group and normal group of 20-39 years old were 1.24 ± 0.17 and 1.15 ± 0.16 respectively.The L/P value of pathological changes group and normal group of 40-59 years old were 1.24 ±0.16 and 1.12 ±0.12 respectively.The L/p value of pathological changes group and normal group of older than 60 years were 1.21 ±0.16 and 1.12 ±0.12 respectively.All of them had significant differences (P ＜ 0.01 ).ConclusionsThe positive rate of chondromalacia patella for female is higher than that for men,which also increases with age.High patella location is relevant to chondromalacia patella.

To study the influence of individualized medication guidance based on an Short Messaging Service( SMS) platform on the medication compliance in the patients with diabetes. Methods: Totally 100 cases of diabetic patients from the outpa-tient section were randomly divided into two groups using a number method with 50 ones in each, namely odd number was in the obser-vation group and even number was in the control group. The observation group was given pharmaceutical guidance based on the SMS technology platform by pharmacists, and the control group was given telephone follow-up once a week to understand the medical situa-tion. At the beginning of the study and after three-month treatment, the medication compliance in the two groups was evaluated, and the control situation of blood glucose was also compared. Results:There was no significant difference in the medication compliance be-tween the two groups at the beginning (P>0. 05). After the 3-month treatment, the blood glucose and medication compliance between the two group had statistical significance (P<0. 01). Conclusion:Based on a mobile phone short message technology platform, indi-vidualized clinical pharmaceutical guidance can significantly improve the medication compliance in diabetic patients.

Acute Disease ; Adolescent ; Humans ; Male ; Myositis

Herba Hedyotis Diffusae has many kinds of pharmacological activities such as anti-tumor, antioxidant, immune regulation and so on. This article collected and reported the related literature at home and abroad in recent years, found that pharmacological active ingredients of Herba Hedyotis Diffusae show consistency of polysaccharides and flavonoids to varying degrees, and summarized the pharmacological effects and extraction and purification process. The author believed that, the study about Herba Hedyotis Diffusae has made relatively great achievements, but the research mainly focused on polysaccharides or flavonoids, and efficacy of Herba Hedyotis Diffusae was not in enough in-depth exploration. The indicator of extraction and purification process is relatively single. This study estimated the mechanism of Herba Hedyotis Diffusae from the aspects of absorption and metabolism of intestinal flora and relationship between medicine structure and exert efficacy, hoping to optimize the extracting processes by multiple indexes, and provided references for further exploitation and utilization of Herba Hedyotis Diffusae.

This study analysed the tissue specific expression of critical genes involved in chlorogenic acid and luteolin biosynthesis, for exploiting the molecular mechanism of components biosynthesis in Lonicera confusa. Expression of PAL, 4CL, C4H, CHS, CHI, FNS and HQT gene families of chlorogenic acid and luteolin biosynthesis-related genes in buds and leaves of L. confusa were analyed by Real-time PCR. Expressions of PAL1, C4H1, 4CL1, CHS1, CHI3 and HQT2 in buds were lower than that in leaves, and expressions of PAL3, 4CL2, CHI2 and FNS2 in buds were higher than that in leaves. The results indicated that that PAL3 and 4CL2 may be associated with accumulation of chlorogenic acid, and the expression patterns of PAL1, CHS1, CHI3 and HQT2 in buds and leaves of L. confusa were different with L. japonica. This study provided some theoretical basis for the further research on genetic mechanism of active components differences in L. confusa and L. japonica.
Biosynthetic Pathways ; Chlorogenic Acid ; metabolism ; Gene Expression Regulation, Plant ; Lonicera ; genetics ; metabolism ; Luteolin ; biosynthesis ; Multigene Family ; Plant Proteins ; genetics ; metabolism

Objective To explore the relationship between temperature and change of serum electrolytes (potassium, natrium, chlorine,and calcium) in patients with spinal cord injury. Methods 321 patients with spinal cord injury (SCI) following fever were regarded as observation group, while 313 SCI patients in normal temperature as control group. The level of serum electrolytes (potassium, natrium, chlorine, and calcium) in both groups was analyzed. Results There was no significant difference in the level of serum potassium (P>0.05), but was in the serum natrium, chlorine, and calcium (P<0.05) between 2 groups. The level of serum potassium didn't correlated with their temperature, and the level of serum natrium, chlorine, and calcium correlated with the temperature negatively. Conclusion The level of serum natrium, chlorine, and calcium may decrease in SCI patients following fever while the level of serum natrium is unchanged.

Objective To investigate the trend of length of stay (LOS) and identify variables that predict LOS for inpatient rehabilitation after stroke. Methods A total of 3049 inpatients who completed the stroke rehabilitation program at Beijing Bo'ai Hospital from January 2002 to December 2011 were reviewed and analyzed with nonparametric rank sum test and binary Logistic regression analysis. Results The median of LOS was 82 days, and tended to decrease obviously in 2010 and 2011. On Logistic regression analysis, there were 13 variables associated with LOS, in which operation, muscle spasm, gain of activities of daily living (ADL), onset admission interval, nosocomial infection,complications, speech disorder, the type of stroke, the number of hospitalization, methods of payment were the risk factors, and age,functional ambulation classificateion and Fugl-Meyer Assessment were protect factors. Conclusion Reducing LOS must base on rehabilitation efficacy, and the improvement of hospital management process, the prevention of complications and nosocomial infection control, and early rehabilitation intervention can reduce the LOS.

OBJECTIVE: To analyze the development of managerial psychology so as to solve culture, managerial system and peculiar problems at the transitional phase.DATA SOURCES: By using CNKI and PSYCINFO database, we searched literature on organization and managerial psychology in the past 20 years. We also collected the authors' research papers related to this area in the recent years.DATA SELECTION: We selected 24 research articles on organizational reforms, leadership behavior, motivation mechanism and organizational culture.DATA EXTRACTION: Seventeen out of the 24 articles were excluded because they overlapped with each other in content to some extent. Therefore,this article reviewed the other 7.DATA SYNTHESIS: On the basis of comprehensive analysis of development of managerial psychology at home and abroad, this article predicts the newly emerging hot issues and trend of research on managerial psychology, and further puts forward the short-term and long-term development goals of managerial psychology research.CONCLUSION: Paying attention to research on organization-level changes,accentuating on the systematic exploitation of human resources, continuously expanding research field and giving more concern for the national goals are the development trend of managerial psychology.