Brain Stem/pathology* ; Death, Sudden/etiology* ; Fatal Outcome ; Humans ; Intracranial Hemorrhages/mortality* ; Leukemia/pathology*

OBJECT: To investigate the changes in the expression_level of synaptophysin following diffuse brain injury (DBI) in rats and to correlate the changes of the synaptophysin expression_level with the post injury time interval. METHODS: Wister rats were used as a DBI model induced by Marmarou method. The changes of synaptophysin immunoreactivity on coronal sections of the rats sampled at different post-injury time intervals were used as a marker. The densitometry of the synaptophysin immunoreactivity was documented by imaging technique and analyzed by SPSS software. RESULTS: The expression level of synaptophysin in DBI rats showed dynamic changes following DBI as well as during the repairing period. CONCLUSION The changes of synaptophysin level may be used as a marker for estimation of the post injury time interval in DBI.
Animals ; Brain/pathology* ; Brain Injuries/pathology* ; Cerebral Cortex/pathology* ; Diffuse Axonal Injury/pathology* ; Disease Models, Animal ; Immunohistochemistry ; Intracranial Hemorrhage, Traumatic/pathology* ; Neurons/pathology* ; Rats ; Rats, Sprague-Dawley ; Staining and Labeling ; Synapses/pathology* ; Synaptophysin/metabolism* ; Time Factors

OBJECTIVE: To apply Fourier transform infrared (FTIR) spectroscopy to study the process of postmortem degradation of the rat brain and to provide a new way for the estimation of postmortem interval (PMI). METHODS: The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at (30 +/- 2) degrees C. To measure the content of the chemical groups in postmortem rat brains at the different time points from 0 to 36 h using the FTIR spectrograph. RESULTS: With prolongation of PMI, the peak position of main absorbance bands in the FTIR spectra showed no significant changes, while the peak levels showed dramatic changes: (1) The relative peak intensity of 1080 cm(-1), 1238 cm(-1) (I1080/I1398, I1238/I1398) associated with nucleic acid decreased obviously; (2) The peak intensity ratio at Amide I, II (I1647/I1541) decreased; (3) The peak intensities at 1456 cm(-1) and 1398 cm(-1) showed a decreased and an increased trend, respectively; (4) Compared to the peak intensity of 1647 cm(-1), the peak intensities at 2852 cm(-1), 2871 cm(-1), 2923 cm(-1), and 2958 cm(-1) tended to increase, with only a slightly increased tendency in peak intensity of 2871 cm(-1). CONCLUSION FTIR spectroscopy may be potentially used as an effective method for estimating the PMI in medicolegal practice using brain tissue sample.
Animals ; Brain Chemistry ; Death ; Forensic Pathology/methods* ; Male ; Postmortem Changes ; Rats ; Rats, Sprague-Dawley ; Spectroscopy, Fourier Transform Infrared ; Time Factors

OBJECTIVE: To explore medico-legal characteristics of sudden death caused by coronary heart disease combined with coronary thrombosis. METHODS: Ninety-six cases of sudden death caused by coronary heart disease were collected and divided into two groups: thrombus positive and thrombus negative groups. The time onset, induction and pathological features of coronary artery disease were analyzed. RESULTS: Two groups showed man-dominant population. There were no statistical significant differences in season, circadian rhythm and induction factor. The thrombus positive group (age < 40) showed a higher disease incidence. Heart weight and degree of coronary stenosis were lower in thrombus positive group. However, there was no statistical difference in the number of atherosclerotic coronary arteries (> or = 2), the length of coronary lesions and myocardial infarct. But thrombosis positive group showed lower tendency. CONCLUSION Two groups are man-dominant population and similar induction factor, lesion position, mechanism of death. But thrombus positive group appeared more in a younger population and the degree of coronary stenosis is milder than thrombus negative group. Forensic pathologists should pay more attention to these characteristics in death investigation.
Adult ; Age Factors ; Aged ; Autopsy ; Cause of Death ; Coronary Artery Disease/pathology* ; Coronary Thrombosis/pathology* ; Coronary Vessels/pathology* ; Death, Sudden, Cardiac/pathology* ; Female ; Forensic Pathology ; Humans ; Male ; Middle Aged ; Myocardial Infarction/pathology* ; Myocardium/pathology* ; Risk Factors