1.Investigation on the endemic foci of new emerged tick-borne encephalitis in Charles Hilary, Xinjiang.
Gui-lin ZHANG ; Ran LIU ; Xiang SUN ; Yang ZHENG ; Xiao-ming LIU ; Yan ZHAO ; Rong-li DANG ; Shuan-kui LIU ; Jing XIA ; Zhong ZHENG ; Yin-hui YANG
Chinese Journal of Epidemiology 2013;34(5):438-442
OBJECTIVETo investigate the recent emerged endemic region of tick-borne encephalitis (TBE) regarding its natural reserves, in Charles Hilary, northern Xinjiang and to isolate and characterize the viral geographic strain.
METHODSUsing indirect fluorescent assay to detect tick-borne encephalitis virus (TBEV) specific IgG antibodies from serum of local residents including 2 unconfirmed viral encephalitis patients in 2011 spring-summer. Viruses were isolated from tick samples by inoculating BALB/c mice and BHK-21 cells. For phylogenetic analysis. TBEV NS1 gene fragments were detected by RT-PCR and then subjected to sequence alignment.
RESULTS1760 ticks were captured from the fields to have found that Ixodes persulcatus were dominated among the tick population. Two viral encephalitis patients were diagnosed as TBEV infection. In addition, 35.4% (23/65) local residents were detected positive for presence of TBEV specific-IgG antibodies in serum. After inoculation, morbidity and mortality of BALB/c mice were 72.9% (70/96) and 55.7% (44/79), respectively. TBEV specific-fragments were amplified from brain tissue of dead mice and cells culture supernatant. NS1 sequence alignment showed that the viral isolates were clustered into TBEV far-eastern sub-type, phylogenetically, and were mostly close to the isolates from northeastern China (99%) and Russian strain (98%).
CONCLUSIONIn this study, a new endemic loci of TBE was firstly described in Charles Hilary natural reserve, northern Xinjiang. TBEV geographic isolates belonged to TBEV far-eastern subtype while Ixodes persulcatus and Dermacentor silvarum played crucial roles for disease transition.
Adult ; Animals ; China ; epidemiology ; Encephalitis Viruses, Tick-Borne ; genetics ; immunology ; isolation & purification ; Encephalitis, Tick-Borne ; epidemiology ; transmission ; virology ; Female ; Humans ; Male ; Mice ; RNA, Viral ; genetics
2.Intervention Effect and Mechanism of Fufang Huangbaiye Tuji on Skin with Deep Ⅱ Degree Burn Wound
Chang-shuan XIAO ; Ya-ping LIU ; Kui SUN ; Jing-zhe YANG ; Hong-Yu CHEN
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(16):102-110
Objective:To study the intervention effect and underlying mechanism of Fufang Huangbaiye Tuji (FFHBY) on skin with deep Ⅱ degree burn wound. Method:Patients with deep Ⅱ degree burn of fire-toxin injuring fluid syndrome diagnosed in the Affiliated Hospital of Chengde Medical University from June 2019 to June 2020 were randomly divided into a control group (iodophor solution, 35 mL per 1% body surface area), a low-dose treatment group (FFHBY, 17.5 mL per 1% body surface area), and a high-dose treatment group (FFHBY, 35 mL per 1% body surface area), 40 cases in each group. The patients in each group were treated correspondingly with dressing chance once per day. The pathological changes of the wound were observed on the 14th day after treatment. Wound symptoms and signs in each group before treatment and on the 7th, 14th, and 21st days after treatment were quantified, and the clinical efficacy on the 21st day after treatment was evaluated. Wound healing rates in each group were calculated on the 7th, 14th, and 21st days after treatment. The levels of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF)-2, FGF-7, epidermal growth factor (EGF), interleukin (IL)-10, tumor necrosis factor (TNF)-
3.Efficacy of Fufang Huangbaiye Tuji on Inflammation on Skin with Deep Ⅱ Degree Burn and Its Dose-effect Relationship
Chang-shuan XIAO ; Ya-ping LIU ; Kui SUN ; Jing-zhe YANG ; Hong-yu CHEN
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(4):88-94
Objective:To observe the effect of different doses of Fufang Huangbaiye Tuji asin the treatment onof the inflammatory response in healing process for of skin with deep Ⅱ degree burn. Methods in healing process. Methods:The 120 cses patients with deep Ⅱ degree burn of fire-toxin injuring fluid syndrome diagnosed in the affiliated hospital of Chengde Medical University between June 2019 and March 2020 were randomly divided into control group,low -dose treatment group and high -dose treatment group,with 40 cases in each group and once. They got a dressing change perevery day. Control group was locally administered with lodophor solution 35 mL per 1% on the body surface area. Low-dose treatment group was locally administered with compound cortex phellodendri fluid 17.5 mL per 1% on the body surface area,while high-dose treatment group was locally administered with compound cortex phellodendri fluid 35 mL per 1% on the body surface area. Observe theThe inflammatory reaction of wound surface in each group onwas observed at admission and after treatment. The pathological changes of each groupsgroup were observed, and determination of nuclear factor kappa-B(NF-