Objective: To study the antimicrobial effectiveness against traditional She medicine-Gardeniae Radix and determine the titer of Gardeniae Radix.Methods: The antibacterial activity of five standard strains(Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Candida albicans and Aspergillus niger)was investigated.A method for the biological value evaluation of Gardeniae Radix was established by using a turbidity method, and compared with the content of chikusetsu saponin Ⅳa determined by HPLC.Results: The sensitivity of Gardeniae Radix to Staphylococcus aureus and Escherichia coli was high.The recovery of the turbidity method was within the range of 90%-110%, and the reliability limit rate was less than 5%.The content of chikusetsu saponin Ⅳa in Gardeniae Radix was not completely consistent with the result of the bioassay method.Conclusion: The bioassay method established in the experiment can be one of the methods for the quality evaluation of Gardeniae Radix.

Objective To explore the characteristics of distribution of TCMsyndrome elements of disease nature between active stage and stationary stage of patients with hepatitis cirrhosis,and to study the corre-lation between the TCMsyndrome elements and serum level of homocysteine (Hcy).Methods By using Information Collection Form ofHepatitis Cirrhosis drafted by our research team,a national multicenter and cross-sectional epidemiological survey was conducted to collect the general condition,symptoms,and blood samples from which the serum Hcy levels were obtained.In accordance with Standard ofHepatitis Cirrhosis Syndrome Elements Differentiation,the TCM syndrome elements of nature of the patients were determined.Then the correlation of the TCMsyndrome elements and the serum levels of Hcy was investi-gated at active stage and stationary stage.Results Altogether 715 patients were included.The frequen-cy of all syndrome elements in patients of active stage were higher than those in patients of stationary phase,in which the element of blood stasis,yin deficiency,qi deficiency,yang deficiency and water re-tention showed statistical differences (P <0.05).The serum level of Hcy in patients of active stage was higher significantly than that of stationary stage (P <0.05).The Hcy level of patients with yin deficiency pattern was lower than that of patients without yin deficiency pattern,the same as element of damp heat, qi stagnation and yang deficiency;however,the situations of element of blood stasis,qi deficiency and water retention were quite the reverse.In the patients of stationary stage,the serum levels of Hcy showed significant difference between qi stagnation group and non qi stagnation group (P <0.05).In the pa-tients of active stage,the levels of Hcy showed difference between yin deficiency group and non yin defi-ciency group,as well as the element of qi deficiency (P <0.05).Conclusion The frequency of TCM syndrome elements and the serum Hcy level in patients of active stage higher than those of patients of sta-tionary stage,reflected the degree of hepatic inflammation and severity of disease.That the correlation between the Hcy level and the TCMsyndrome elements of nature could provide the evidence for pathologi-cal basis and standardization study of TCMsyndrome elements in patients with hepatitis cirrhosis.

Objective To analyze the distribution of diseases with TCMpattern of spleen-stomach damp-ness-heat (SSDHP)and the prescriptions and Chinese herbal medicines involved,so as to guide TCM clinical practice and further research on SSDHP.Methods Taking “SSDHP”(Chinese:脾胃湿热证) as subject term or title /keyword,the databases of China National Knowledge Infrastructure(CNKI),Wan-fang Data Knowledge Service Platform and VIP Information were systematically retrieved,from the begin-ning of the databases to 31 December,2015.The articles related to treatment on SSDHP were collected, and the database was established by using Microsoft Excel 2010,on which data were descriptively statisti-cal analyzed.Results 109 articles were eligible from total 1 681 retrieved ones.The top two diseases with SSDHP were digestive system disorders (86.41%)and dermatoses (5.83%).The common formu-la directed at clearing heat and dampness including Pingwei Powder,Huopu Xialing Decoction,and Sanren Decoction,combined with specific medications for exact symptoms.The common Chinese herbal medications with frequency more than 1 percent were listed as the following:Golden Thread (Huangli-an),Pinellia Tuber(Banxia),Official Magnolia Bark(Houpu),Liquorice Root (Gancao),Dried De-coctionerine Peel (Chenpi),Baical Skullcap Root (Huangqin),Indian Bread (Fuling),Dandelion (Pugongying),Agastache Rugosus (Huoxiang),Atractylodes Rhizome (Cangzhu),Coix Seed (Yiy-iren),Round Cardamon Fruit (Baidoukou),Largehead Atractylodes Rhizome (Baizhu),Orange Fruit (Zhiqiao),Immature Orange Fruit (Zhizi ),Corydalis Rhizoma (Yanhusuo ),White Peony Root (Baishao),Medicinal Evodia Fruit (Wuzhuyu),Dried Ginger (Ganjiang),Bamboo Shavings (Zhu-ru),Virgate Wormwood Herb (Yinchen),Grassleaf Sweetflag Rhizome (Shichangpu),Turmeric Root Tuber (Yujin),Immature Orange Fruit (Zhishi),Chinese Thorowax Root(Chaihu),Common Bletilla Tuber (Baiji),Danshan Root (Danshen),Bitter Apricot Seed (Kuxingren),Villous Amomum Fruit (Sharen),Common Aucklandia Root (Muxiang),Finger Citron (Foshou),Talc (Huashi).Conclu-sion Now,the diseases with SSDHP were most common in digestive system,but also in other systems. And the prescription and Chinese herbal medicines should be applied by holistic conception,combination of disease and pattern and pattern differentiation and treatment.Classic herbal formulas in TCM ancient books are still playing the strictly key roles in clinical practice,but proved formulas of modern outstand-ing senior traditional Chinese practitioners and Hospital Preparations also are paid more attention by re-searchers.

To explore the significance of Erbin expression in esophageal squamous cell carcinoma (ESCC) and its relation-ship with patient prognosis. Methods: Erbin expression in a tissue chip containing samples from 299 cases were examined using immu-nohistochemistry; in addition, the relationship between Erbin expression and clinical-pathological parameters and patient survival time were also analyzed. Cox regression was used to predict the risk of clinical-pathological parameters. The mRNA and protein expres-sion of Erbin was also determined in 25 cases with paired ESCC and normal tissues through polymerase chain reaction (PCR) and West-ern blot. Results: The expression of Erbin protein and mRNA in ESCC were significantly higher than those of normal esophageal epithe-lium adjacent to cancer (55.2% vs. 0, P<0.05). The high expression of Erbin in ESCC was closely related to TNM stage (64.9% vs. 47.3%, P=0.002) and lymph node metastasis (65.5% vs. 45.0%, P<0.001). Moreover, the high expression of Erbin in ESCC was closely related to poor prognosis (P<0.05). Conclusions: Erbin expression was increased in ESCC and was closely related to poor patient prognosis, which suggested that Erbin may be an important biomarker for the prognosis of cancer patients.