1.Establishment of a rat model of craniocerebral blast injury induced by cabin explosion
Yan-Teng LI ; Gang CHENG ; Shuai LIU ; Bang-Xin LIU ; Shu-Wei WANG ; Bo-Yuan WEI ; Han-Ding MAO ; Jian-Ning ZHANG
Medical Journal of Chinese People's Liberation Army 2017;42(9):820-825
Objective To establish a rat model of craniocerebral blast injury caused by the shock wave of cabin explosion.Methods Fifty male adult Sprague-Dawley rats were randomly divided into 5 groups (10 each):3g,5g,8g TNT with vest groups,5g TNT without vest group and control group.Uncased explosives of different equivalent were suspended in the cabin center.After anesthesia,with exception of control group,the rats were placed in prone position about 31 cm below the explosive,facing the explosive with or without vest.After the explosion,the survived rats were observed,serological and pathological examinations were performed at 3h,1d and 3d after the explosion.Results In terms of tissue damage and mortality,compared with the control group,no obvious injury formed in rats of the 3g TNT with vest group,and all of them survived;Rats in 5g TNT with vest group showed mild lung injury,brain tissue edema,enlarged blood vessel,patchy hemorrhage on the brain surface,and with a mortality of 30%;Rats in 8g TNT with vest group showed serious organ damage with a mortality of 80%;Rats in 5g TNT without vest group suffered from severe lung injury,almost all died right after the explosion.Therefore,rats in 5g TNT with vest group were more in line with the experimental needs.Further serum and pathologic examinations showed that the brain water content increased,the serum neuron specific enolase (NSE) and S-100β protein also increased markedly,and necrotic or apoptotic changes happened in the cortex and hippocampus neurons.Conclusion A stable animal model of craniocerebral blast injury may be established with rats in the case of chest and abdomen protected and then exposed to 5g TNT explosion in cabin.
2.Injury characteristics and injury analysis in Beagle dogs after multi-cabin explosion
Shuai LIU ; Gang CHENG ; xin Bang LIU ; teng Yan LI ; wei Shu WANG ; yuan Bo WEI ; Bo JIA ; nan Ya LIU ; ning Jian ZHANG
Medical Journal of Chinese People's Liberation Army 2017;42(11):1011-1015
Objective To investigate the injury characteristics of Beagle dogs after warship multi cabin explosion.Methods Forty-eight adult male Beagle dogs were placed in the simulated blast-cabin and adjacent cabin (24 each),and ammunitions respectively containing 0.75kg TNT and 3.50kg TNT were then detonated in the blast-cabin.The survival situation,fluctuation of vital signs,morphological changes of organs and the incidence of various types of injury of the dogs were observed immediately after the explosion to 24h after injury,and the neurological functions score was performed.Results Twenty dogs died immediately after the explosion,and another 9 dogs died 24h after the explosion.The total mortality was 60.42%(29/48),and the mortalities in blastcabin and adjacent cabin were 79.17%(19/24) and 41.67%(10/24),respectively.The dog's skull was penetrated by bomb fragments,and congestion and bleeding were observed in brain tissue,lung,heart,stomach,bowels,liver and kidneys.Extremities fracture,soft tissue contusions,perforation and rupture were also checked out after explosion.The fatality rate of bomb fragment injury,blast injury and combined bomb fragment-blast injury was 27.59%(8/29),17.24%(5/29) and 55.17%(16/29),respectively.Conclusions The combined bomb fragment-blast injury show high incidence and make high fatality rate and serious injury.Combatants should effectually shield themselves with occluded objects as far as possible to avoid damage and reduce fatality.The key of early treatment is to treat the multiple injuries promptly.Intravenous fluid therapy should be practiced after hemodynamic monitoring.
3.Efficacy of Baxian Xiaoyaotang Against Ankylosis of Wind-Cold-Dampness Obstruction Syndrome After Acute Achilles Tendon Rupture Surgery and Its Influences on TGF-β1, IGF-1, and EGF
Shuai-bang CHENG ; Yu-jing CAO ; Xiu-chun QI ; Yang LI ; Zi-bin ZHU ; Chao LIU
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(12):103-110
Objective:To investigate the efficacy of Baxian Xiaoyaotang (BXT) in treating ankylosis of wind-cold-dampness obstruction syndrome after acute Achilles tendon rupture surgery and its effects on transforming growth factor-