To probe into problems in the existing essential medicine system in China and the necessity of legislature in this regard, and propose to build a legal framework for such a system. The author holds that the administrative regulations formulated by the State Council can ensure the promotion and implementation of the essential medicine system effectively.

Malunion is one of common complications after distal radius fractures.It is indicated that malunion plays an important role in fracture recovery in many studies.This article sums up the research data,from aspects of both biomechanical analysis and follows-up of clinical cases,of domestic and foreign scholars in recent years,and reviews the influences of malunion on wrist joint functional outcome.

"Conducting yang from yin, and conducting yin from yang" is the essential principle of acupuncture and moxibustion in treatment of diseases, originated from Neijing (Internal Classic). In clinical, yin and yang are not just limited as the natures of meridians and zangfu organs, but also refer to the locations of acupoints, such as the upper and the lower, the left and the right, the anterior and the posterior, the exterior and the interior. Besides, they include the time of acupuncture, such as four seasons and midnight-midday ebb flow, and the manipulation of acupuncture, etc. The ultimate goal of acupuncture is to regulate yin and yang.
Acupuncture Points ; Acupuncture Therapy ; Humans ; Meridians ; Seasons ; Yin-Yang

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Torticollis ; therapy

This study was aimed to find the correlation between amino acid compositions and Cold-Heat Nature of traditional Chinese medicine (TCM) in order to provide basis for the research of TCM natural theory. A total of 17 kinds of amino acid were determined by application before column derivatization reaction and high-perfor-mance liquid chromatography (HPLC) and the tryptophan was determined by UV method. The data were collected for analysis by Fisher method in PAST software. The best statistical identification model was determined. And the Cold-Hot medicine property markers (CHMP-markers) were determined. The results showed that the discriminant function established by Fisher method based on 18 kinds of amino acid contents has good identification ability, and the accuracy of the Fisher discriminant analysis is 82%. Support vector machine (SVM) is the best statistical identification model . The cold and heat markers were analyzed by SVM . The cold nature material bases in-clude Glu, Gly, Arg, Thr, Ala, Tyr, Val, Ile and lys. And the heat nature material bases contain Asp, Ser, His, Pro, Met, Cys, Leu, Phe and Trp. It was concluded that there is relationship between 18 kinds of amino acid contents and the Cold-Heat nature of TCM .

Objective To confirm that astrocytes from cerebral cortex of newborn rat can be the target cells of Hantaan virus (HTNV)and Seoul virus (SEOV)infection and to observe changes of astrocytes after different infection time. Methods Astrocytes were prepared from cerebral cortex of newborn rat, and then infected with HTNV and SEOV. The established virus infections were confirmed by detection of virus nucleocapsid protein (NP) and S segment RNA in astrocytes using double-label immunofluoreseence, Western-blot and reverse transcriptase-polymerase chain reaction (RT-PCR). Results The astrocytes from cerebral cortex of newborn rat were cultured successfully in vitro, which could be infected by HTNV and SEOV. The number of infected astrocytes and the virus titer in the infected astrocytes kept on increasing along with the extended infection duration. Conclusions Astrocytes from cerebral cortex of newborn rat are the target cells for HTNV and SEOV infection. Then establishment of in vitro cultured astrocytes model for Hantaviruses infection will be helpful for the study on the pathogenesis of hemorrhagic fever with renal syndrome.

Acupuncture Therapy ; instrumentation ; methods ; Adult ; Aged ; Facial Paralysis ; therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome

Objective To explore the relationship between TSLC-1 protein expression and the progression and carcinogenesis of human papillary thyroid carcinogenesis. Methods Expressions of TSLC-1 protein in 20 pieces of benign thyroid lesions and 40 pieces of papillary thyroid carcinoma tissues were examined by immunohistochemistry. Results The expression of TSLC-1 in papillary thyroid carcinoma (9/40) was lower than that in benign thyroid lesions (22.50% versus 90.00%, P < 0.05). Expression of TSLC-1 was significantly associated with lymph node metastasis and TNM in papillary thyroid carcinoma (P < 0.05). Conclusions Expression of TSLC-1 is closely related with carcinogenesis , lymph node metastasis , and clinical stage in papillary thyroid carcinoma.

Objective To explore the difference of ocular biological measurements between type 1 and type 2 diabetic patients with proliferative diabetic retinopathy.Methods A total of 66 type 2 diabetic patients (67 eyes) with PDR (aged 37-77 years old),10 type 1 diabetic patients (19 eyes) with PDR (aged 18-57 years old) and 35 idiopathic macular hole patients (35 eyes,aged 21-74 years old) from September,2014 to July,2016 in our hospital were retrospectively analyzed,and the differences of axial length,corneal endothelial cell and age among three groups were compared.Results The average axial length of type 1 diabetic patients with PDR was (21.74 ± 0.68) mm,type 2 diabetic patients with PDR was (23.06 ± 0.65) mm,idiopathic macular hole patients was (23.39 ± 0.65) mm,the difference was statistically significant (P ＜ 0.001).There was no statistical difference in average corneal endothelial cells number among three groups (P =0.103),but there was statistical difference in the age (P ＜ 0.001).Conclusion The axial length in patients with type 2 and type 1 diabetic patients with PDR are less than the normal,while type 1 diabetic patient is shorter than type 2 diabetic patient,and the onset time of type 1 diabetic patients with PDR is earlier than that of type 2 diabetic patients with PDR.

Objective To establish a HPLC method to determine the content of acteoside in Conghuang Bushen Capsule. Methods The HPLC method was employed with a column of Hypersil ODS2(4.6 mm? 250 mm,5 ? m) and a mobile phase of acetonitrile-methanol-1 % HOAc(13 ∶ 9 ∶ 78),the detection wavelength being at 334 nm,flow rate being 1.0 mL/min and the column temperature being at 40 ℃ . Results The linear range was 0.108 5 ? g ～ 0.650 7 ? g,r=0.999; 1.The average recovery of acteoside and RSD were 98.42 % and 1.89 %,respectively. Conclusion The present method is simple,rapid and reproducible. It can be used for quality control of Conghuang Bushen Capsule.