BACKGROUND:Bone marrow stromal stem cels transplanted into infracted cardiac tissue can inhibit and reduce myocardial apoptosis, but whether this effect is correlated with improvement in cardiac function is stil unclear. OBJECTIVE:To study the early effect of bone marrow stromal stem cels transplanted into the ischemic myocardium on the cardiac function. METHODS: Models of acute myocardial infarction were established by ligation of the left anterior descending branch, while no ligation was done in the sham group. In the transplantation group, rat bone marrow stromal stem cels (0.1 mL, 2×106 RESULTS AND CONCLUSION:At 3 days after cel transplantation, myocardial apoptosis was more evident in the infarct and ischemic zones of the model group than the sham group; the number of apoptotic myocardial cels was significantly lower in the infarct and ischemic zones of the transplantation group than the model group. Compared with the sham group, the mean arterial blood pressure and left ventricular systolic pressure were ) were injected into five sites on the edge of infarcted myocardial tissues at 30 minutes after myocardial infarction. In the sham group and model group, the same volume of normal saline was injected into the myocardial tissues. Three days after cel transplantation, hemodynamic monitoring, echocardiography, TUNEL assay were employed to detect myocardial apoptosis. significantly reduced, the left ventricular end diastolic pressure was increased, and the left ventricular ejection fraction and shortened fraction were significantly lowered in the model and transplantation groups (P < 0.05), but there was no difference between the model and transplantation groups (P > 0.05). These findings indicate that myocardial apoptosis can be reduced but the cardiac function cannot be improved in acute myocardial infarction rats at early stage after bone marrow stromal stem cels transplantation.

Pimavanserin is a selective 5-HT2A receptor inverse agonist approved by FDA on April 29, 2016, and it is the first drug for the treatment of hallucinations and delusions associated with Parkinson' s disease. Pimavanserin is with high safety, good toleration and promising clinical efficiency, which is a timely option to resolve the drug shortage in clinic treatment. This paper summarized its pharmacodynamics, pharmacokinetics, clinical studies, adverse reactions and drug interactions.

The Health Anti-poverty Project is a main and effective method for raising the health level of poor areas people and realizing the Healthy China.It is important to construct and improve the health service system of poverty-stricken area by means of strengthening construction of standardized medical and health institutions,public health service network,telemedicine service system,and Chinese medicine service ability,aiming at forming the health service system which is compatible with the local economic development and health needs.The system can ensure the health of people in poverty-stricken areas,and implement the Health for All in China.

As a member of nuclear receptor superfamily, farnesoid X receptor (FXR) has been shown to regulate numerous metabolic pathways, which include playing an important role in bile acid metabolism, maintaining lipid and glucose homeostasis when FXR is activated. With the prevalence of the glucose and lipids disorder, FXR attracts increasing attention. It may be a potential target for the treatment of type 2 diabetes mellitus and lipid disorders.
Bile Acids and Salts ; Diabetes Mellitus, Type 2 ; Glucose ; metabolism ; Homeostasis ; Humans ; Lipid Metabolism ; Receptors, Cytoplasmic and Nuclear ; metabolism

Objective:To establish an HPLC method for the determination of related substances in piperazine ferulate. Methods:An HPLC method was used to determine the related substances in piperazine ferulate. The separation was performed on an Xtimate C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was 0. 5% acetic acid-methanol-acetonitrile with gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was 30℃. The detection wavelength was 286 nm and the injection volume was 20μl. Results:Ferulic acid had a good linear relationship within the range of 5-30 μg·ml-1(r=1.0000). The detection limit was 0. 02 ng. Conclusion:The method is reliable, simple, accurate, stable and durable, and suitable for the determination of related sub-stances in piperazine ferulate.

BACKGROUND:The third molar extraction may lead to different degrees of soft tissue damage. Ultrasound and ultrashort waves belong to hyperthermia, which can increase tissue temperature and improve the therapeutic efficacy of soft tissue stretch. OBJECTIVE:To observe the effects of soft tissue stretch combined with ultrasound and ultrashort wave therapy for patients who were recently diagnosed with trismus and pain folowing third molar removal. METHODS:Sixteen patients with trismus and pain after third molar removal were randomly divided into two groups: patients in stretch group received soft tissue stretch with ultrasound and ultrashort wave therapy, while those in control group received only ultrasound and ultrashort wave therapy. Patients received the treatment five times per week, totaly for 2 weeks. RESULTS AND CONCLUSION:It was found that after treatment pain was significantly reduced at the temporomandibular joint, and the range of maximum active mouth opening increased significantly. The improvement in mouth opening and temporomandibular joint function was better in the stretch group than the control group. These findings indicate that ultrasound and ultrashort wave therapy can improve symptoms of trismus and pain folowing third molar removal, and the combination of ultrasound and ultrashort wave therapy and soft tissue stretch therapy can achieve more effective results.

ObjectiveTo evaluate the effects of all-trans retinoic acid(ATRA) on melanin content,activity and protein expression of tyrosinase,mRNA expression of Cu/Zn superoxide dismutase(SOD) in A375 cells irradiated with ultraviolet B(UVB).MethodsCultured A375 cells were classified into 6 groups:ATRA+UVB group treated with ATRA after UVB irradiation,hydroquinone+UVB group treated with hydroquinone after UVB irradiation,UVB group and ATRA group treated with UVB irradiation and ATRA respectively,negative control group receiving no treatment.Melanin content and tyrosinase activity were determined by NaOH solubilization assay and dopa-oxidation assay respectively at 24,48 and 72 hours after the addition of ATRA into medium.Western blot was performed to detect the protein expression of tyrosinase,and real-time quantitative PCR to measure the mRNA expressions of tyrosinase and Cu/Zn SOD in A375 cells after 24-hour culture with ATRA.ResultsThe melanin content and tyrosinase activity decreased in UVB-irradiated cells after being treated with ATRA for 24,48 and 72 hours.The protein (gray scale) and mRNA (2-△△Ct value) expression levels of tyrosinase were 0.72 ± 0.070 and 1.400 ± 0.135 respectively at 24 hours after UVB irradiation,decreased to 0.42 ± 0.056(P ＜0.01) and 0.810 ± 0.062(P ＜ 0.01 ) respectively after additional treatment with ATRA.The mRNA expression level of Cu/Zn SOD was 0.323 ± 0.066 in A375 cells at 24 hours after UVB irradiation,and increased to 0.625 ±0.103 (P ＜ 0.01 ) after additional treatment with ATRA.ConclusionATRA can suppress UVB-induced increase in melanin synthesis and elevate Cu/Zn SOD level in A375 cells,likely through tyrosinase pathway.

ObjectiveTo establish a central nervous system axonal mechanical transection model in vitro and observe the axonal regeneration style and speed following the transection. MethodsThe cortical explants the mice were cultured in vitro,of which the axon and dendrite parts were marked using immunofluorescence.The model was built by mechanically transecting the axons and removing the severed axons.The axonal regeneration style was observed by tracing the undeveloped cellular bodies adhering to the residual axonal surface.The growth speed of the regenerated axons and normal axons were measured as well.Results ( 1 ) After mechauically transecting the axons of explants,the axonal regeneration was largely founded at the transected line.The undeveloped cellular bodies went over the transected line and spread to the distal area with axonai regeneration. (2) The axonal growth speed was (118 ± 32) μm/d and (72 ±41) μm/d in the transection group,but was (41 ± 17) μm/d and (32 ± 19) μm/d in the control group at 24 and 48 hours respectively. ConclusionThe regeneration style of the transected axons is the extension of the axonal stumps,rather than sprouting growth,and the growth speed is faster than that of the normal axons.

Adult ; Chronic Periodontitis ; diagnosis ; diagnostic imaging ; therapy ; Combined Modality Therapy ; Dental Implantation ; Dental Scaling ; Female ; Follow-Up Studies ; Guided Tissue Regeneration, Periodontal ; Humans ; Orthodontics, Corrective ; Radiography, Panoramic ; Tooth Loss ; diagnosis ; diagnostic imaging ; surgery ; Toothbrushing

Objective: To detect the expression of CDX2, COX-2, and NF-κB in the tissues of gastric carcinoma (GC), precancerous lesions, and normal gastric mucosa and to in vestigate the correlation among CDX2, COX-2, and NF-κB. Methods:Data on the expression of CDX2, COX-2, and NF-κB were evaluated using immunohistochemistry in 60 cases with GC, 45 cases with precancerous lesions, and 20 cases with normal tissues. The protein levels in different pathological tissues, as well as their correlation with GC, were analyzed. Results:The positive rates of NF-κB and COX-2 proteins were significantly higher in GC group than those in precancerous lesion and normal tissue groups (P<0.05). CDX2 was expressed in GC and precancerous lesion groups but poorly expressed in the normal mucosa. The positive rate of CDX2 expression was significantly higher in precancerous lesion group than that in GC group (P<0.01). NF-κB expression was positively correlated with COX-2 in GC (P<0.01), whereas CDX2 expression was negatively correlated with COX-2 and NF-κB in GC (P<0.01). Conclusion:CDX2, COX-2, and NF-κB are possibly correlated with one another, and they cooperate during the onset, progression, and metastasis of GC.