Objective To investigate the changes in the detection rate of gastric ulcer,duodenal ulcer and gastric cancer by gastroscopy after 10 years' eradicative treatment of Helicobacter pylori,in order to learn the characteristic alternations of these diseases and the significance of eradicative treatment of Helicobacter pylori.Methods The clinical and endoscopic data of gastric ulcer,duodenal ulcer and gastric cancer at our hospital in January 1994 to December 1995 and January 2004 to December 2005 were collected and analyzed.Results The detection rate of gastric ulcer,duodenal ulcer,and gastric cancer by gastroscopy during the period of January 2004 to December 2005(5.53%,10.07% and 4.03%,respectively) was significantly lower than that during the period of January 1994 to December 1995(8.17%,11.21% and 6.21%,respectively,P

Objective To study the influences of risk factors on the 2-week mortality in patients with acute cerebral hemorrhage.Methods From January 2004 to June 2007,a total of 65 patients with intracerebral hemorrhage in Beijing Red Cross Hospital were studied.Logistic regression was performed to analyze the relationship between prognostic factors and 2-week mortality.These factors included sex,age,volume of hemorrhage,location of hematoma,the highest body temperature in the first 24 h,and consciousness state on admission etc.Results The in-hospital mortality in 2 weeks was 18.5%(12/65).The volume of hemarrhage,body temperature and consciousness state were probably significant factors.Conclusion The increasing body temperature in the first 24 h and the unconsciousness may be the main risk factors which are associated with high 2-week hospital mortality in patience with intracerebral hemorrhage.

At present, due to the opening and circulation of salt market, potassium iodate iodized salt, potassium iodide iodized salt, seaweed iodized salt and non-iodized salt may exist simultaneously in the edible salt market of a region, which brings new problems to the work of testing iodine content of edible salt in the laboratory. In order to carry out salt iodine detection for prevention and monitoring of iodine deficiency disorders, this paper introduces some methods which established by the author for a rapid qualitative, semi-quantitative and quantitative detection of iodized salt of different forms of iodine and non-iodized salt. And further application and discussion of the methods are described for reference of iodized salt monitoring.

Objective:To establish adoptive transfer model mice of type 1 diabetes for investigating the pathogenesis of type 1 diabetes.Methods:16 NOD.Scid mice were randomly divided into two groups.One was injected i.p. with splenocytes which were obtained from a diabetic NOD mouse and the other was injected with splenocytes obtained from a normal one.The blood glucose levels and weight were detected daily.The mice were killed when they had significant clinic appearance or 10 weeks after cell injection,followed by pathologic studies and cytokine assays.Results:The incidence of adoption group was 8/8 whereas that of control group was 0/8.The pathology scores of 2 groups were 2.5?0.2,0 separately.The amounts of IL-2,IL-10,IFN-? were 60.7 pg/ml,15.5 pg/ml and 20.2 ng/ml respectively in adoption group,and those in control group were 2.4 pg/ml,17.5 pg/ml,3.2 ng/ml.Conclusion:Model mice of type 1 diabetes can be established in NOD.Scid mice during the 5-10 week after adoptive transfer of splenocytes of diabetic NOD mice.

Objective To explore the histological morphological features of complications following liver transplantation. Methods In 639 patients with complications following liver transplantation, the percutaneous liver biopsy tissues were stained by HE method. Van Gieson, Masson, PAS, reticulin and immunohistochemical staining were performed. HBsAg, HBcAg, HCVAg, CMV-EA, CMV-LA, CMV pp65, EBVAg and the expression of CK19 were detected. The rejection was graded according to Banff standard and scored with RAI.Results 906 times of liver biopsies in 639 cases were performed. Acute cellular rejection (ACR) was most commonly seen in 386 cases ( 42.61 %), followed by complication of biliary tract (251 cases, 27.70 %), drug-induced liver damage (72 cases, 7.95 %), CMV infection (55 cases, 6.07 %), infection or recurrence of hepatitis virus (B or C) (45 cases, 4.97 %), ischemia-reperfusion injury (42 cases, 4.64 %), chronic rejection (32 cases, 3.53 %), obstruction of efferent tract (6 cases, 0.66 %), recurrence of the primary affection (5 cases, 0.55 %), and non-function of live grafts (4 cases, 0.44 %). The complications in 8 cases were difficult to diagnose ( 0.88 %). Compared with the previous report of complications, the incidence rate of ACR was decreased and that of complication of biliary tract and drug-induced liver damage increased in this study. Conclusion Percutaneous liver biopsy is valuable for the diagnosis of complications. It provides the evidence of settling plan of treatment and improves the survival rate.

Objective To observe the inhibition effect of recombinant human monocyte chemoattractant protein－ 1(MCP－ 1) on implantation and growth of human osteosarcoma cells. Methods The method of protein fusion was used for the expression of MCP－ 1 in b.coli and then the MCP－ 1 was extracted and purified. Fifty nude mice were divided into 10 groups. For A1－ 4 groups, the 5 mice in each group were locally injected with doses of 1? g, 10? g, 100? g, 1 mg of MCP－ 1 at the same time when 4.4? 106 osteosarcoma cells were implanted in vivo. For B0－ 4 groups, 5 mice in each group, the injection were given 2 weeks later when there was the formation of the tumor mass at doses of 0? g(0.2 ml normal saline), 1? g, 10? g, 100? g, 1 mg of MCP－ 1 every other day. Five mice in group C were injected with dose of 0.2 ml NS as control. Results Implantation of osteosarcoma cells were completely prevented among mice of group A2－ 4, tumor inhibiting effect even in group A1 and the rate of tumor inhibition was 69.69％ . AKP values in mice of group A1－ 4 were much lower than those of group B0(P

Objective To explore the effectiveness of using middle-latency somatosensory evoked potentials (MLSEP) to predict the prognosis in patients with acute severe stroke. Methods MLSEP, shortlatency somatosensory evoked potentials (SLSEP), and Glasgow Coma Scale (GCS) were recorded in 70 acute severe supratentorial stroke patients within 1 week after onset. All patients were evaluated with modified Rankin Scale (mRS) and follow-up in 6 months after onset. SLSEP and MLSEP were recorded in 20 normal controls. The consistency between MLSEP, SLSEP, GCS and prognosis, as well as the prognostic authenticity of MLSEP, SLSEP, and GCS were analyzed. Results Bilateral N20, N35, and N60 exited in all normal controls. Some waves of MLSEP were absent in stroke patients, and the proportion of absent waves in ipsilateral MLSEP was higher than in contralateral MLSEP. The consistency between bilateral absence of N60 and unfavorable outcome ( Kappa = 0.828, P ＜ 0.01 ), and between bilateral absence of N60 and death ( Kappa = 0.686, P ＜ 0.01 ) was satisfactory. By using the prognostic authenticity analysis of predictors, the ipsilateral absence of N60 showed the highest sensitivity ( 100% ) for unfavorable outcome and death, which added 14.3% compared with the sensitivity of ipsilateral absence of N20 ( 85.7% ). Bilateral absence of N60 showed a high specificity of 100% for unfavorable outcome, which equaled bilateral absence of N20.However, it showed a lower specificity ( 82.9% ) for death, than bilateral absence of N20 (97.1% ).Conclusions MLSEP was able to reflect the degree of brain injury and showed higher sensitivity than SLSEP for predicting unfavorable outcomes. Therefore combined use of MLSEP and SLSEP in evaluating and predicting the outcomes in brain injuries is suggested.

Objective To evaluate a blood test,UniCAP system(screening IgE to food or inhalant allergens,eosinophil cationic protein,ECP)in differentiation the capability of lgE-mediated allergic diseases in children. Methods 26 atopic dermatitis,33 asthma/bronchiolitis,20 allergic purpura, 15 Kawasaki disease patients and 20 normal children were tested total lgE,lgE to food or inhalant allergens,ECP by UniCAP system and compared.Resuits Fx5E positive rate are 73.1%,24.2%,25.0%,13.3%in atopic dermatitis,asthma,allergic purpura,Kawasaki disease respectively,and the Phadiatop positive rate are 23.1%、69.7%、10.0%、6.6%.The serum levels of ECP、TIgE of patients were higher than the mormal controls,with the highest level in asthma, and lowest in Kawasaki disease,the differences were significant(P<0.05).Conclusions Food orinhalant allergens plays an important role in the inflammatory process of asthma,atopic dermatitis,and some role in allergic purpura,Kawasaki disease.Food allergens are more important to atopic dermatitis.and inhalant allergens are more important to asthma.

Objective To analyze the implementation effects of peer-assisted learning on critical thinking ability of undergraduate nursing students. Methods A total of 411 college nursing students were selected with 203 students of Class One, Class Three, and Class Five from Grade 2012 majoring in nursing were taken as the control group, 208 students of Class Two, Class Four, and Class Six as the experimental group. In the course of basic nursing practice, the control group was trained by the traditional practice, and the experimental group was trained by the way of peer mutual aid. Critical thinking disposition inventory Chinese version (CTDI-CV) was adopted to evaluate the level of critical thinking ability of the nursing students of both groups. SPSS 17.0 was used and the data of the two groups were compared by t test. Results The score of experimental group students' critical thinking ability and the score of 7 dimensions including their seeking truth, analysis ability, self-confidence, inquisitiveness, cognitive maturity, open mind and systematic ability were higher than those before the experiment (P<0.05). The total score of the experimental group students' critical thinking ability and the score of 5 dimensions including seeking truth , analysis ability, self-confidence, curiosity and cognitive maturity were higher than those of the control group (P<0.05). Conclusion Peer-assisted learning is beneficial to the improvement of critical thinking ability of nursing undergraduate students.

Aim To explore the vasodilative effect on rat thoracic aortic ring of total flavonoids of Buckwheat flowers and leaves(TFBFL) and the underlying mechanisms.Methods Isometric tension measurements were used to study the effect of TFBFL on isolated rat thoracic aorta rings.Laser scanning confocal microscope was employed to measure the concentration of intracellular free calciums.Results In aorta rings precontracted with phenylephrine or potassium chloride,TFBFL caused a dose-dependent relaxation in both endothelium-intact and denuded rings and the relaxant effect of TFBFL was more potent on endothelium-intact aorta rings than that on endotheliumdenuded aorta rings(P