Clinical Medicine ; Humans ; Neoplasms ; prevention & control ; Periodicals as Topic ; Research

A total of 115 patients with Hoshimoto thyroiditis were classified under 2 groups: one group being treated with selenium and L-throxine (L-T4) and other group with L-T4 alone.All patients were followed up for 3 months.In the selenium treated group thyroid peroxidase antibody level was decreased in 33 patients (56%) and thyroglobulin antibody level was decreased in cases.

Objective To verify the effectiveness of electrical impedance tomography (EIT) with multi-layer electrodes in gastric emptying measurement by simulation experiments using EIT with three-layer electrodes.Methods Firstly,EIT system with three-layer electrodes and gastric emptying measurement simulation equipment was designed and constructed to simulate gastric emptying process and obtain EIT reconstruction images.Secondly,the region where the electrical characteristic of gastric changes was selected as region of interest.Finally,the relative impedance change rate in region of interest for every image obtained from all three layers was consecutively recorded,then the gastric emptying curve was formed and gastric emptying time was calculated.Results There are obvious differences among the sharp of impedance change curves of interested region for three layers,and the gastric emptying time and process of each layer is also different from one another.It is suggested that the passing path and emptying manner of food in gastric emptying process reflected by EIT images varied from layer to layer.Therefore,the necessity and feasibility of using EIT with three-layer electrodes in gastric emptying measurement study is verified.Conclusions EIT with three-layer electrodes can provide more abundant information of gastric motility function associated with gastric emptying pathology and physiology state.It is likely to be a noninvasive,convenient and effective imaging method for the detection and evaluation research on gastric emptying and gastric motility.

Eight-weeks old C57BL/6J male mice were treated with normal or high fat diet for4 weeks,and then bone mass,number and activity of osteoclasts,differentiation of osteoblasts from bone marrow mesenchymal cells and the change in lipid genes expression were studied.Compared with the normal diet treated group,bone mass of femur from high fat diet treated animal was decreased,but there were increased number and activity of osteoclasts and raised lipid genes expression levels.Besides,no obvious change was found in differentiation of osteoblasts from bone marrow mesenchymal cells.The loss of bone mass induced with high fat diet in young mice was mainly due to increased activity of osteoclasts and bone resorption.

Objective To study the phase correlation of cutaneous multichannel impedance measurements and the contractions, peristalsis of stomach and the conduction properties in space.Methods A spiral gastric body model was rotated to simulate shrinkage and creep of the stomach.In order to verify the feasibility and validity of the multichannel impedance gastric dynamic measurement, experiments with emulation of multichannel impedance gastric motility measuring principle were conducted through three-dimensional simulation on the electrical characteristics of gastric body model in saline tank simulator.Results The emulation results showed that the change of epigastric current density and potential distribution is consistent with the course of moving from top to bottom when lateral edge of model (namely the intumescent location when the stomach is full) after detecting electrodes in sequence.Meanwhile, the measurement voltage wave forms clearly showed the contraction location of stomach after the model rotating for three cycles in a row, and the phase relationship between adjacent measurement channels was also measured.The sixty seconds data acquired by the experiment device proved that voltage wave form of three measurement channels corresponded to the phase relationship of movement status of the gastric body model.Conclusions Cutaneous multichannel wave forms prove a cyclical movement of stomach, and phase difference among the multichannel signals relating to speed of peristaltic wave, position of the measuring electrodes and channel spacing, etc.This study provides simulation and experimental basis for explanation of quantitative study of conduction properties in space and phase relationship between the results of surface multichannel impedance measurements and the contractions, peristalsis of stomach.

Objective To explore the effect of lymph node metastases in gastric cancer on vagniae vasorum of gastric vessels,in order to provide evidence for the surgical treatment of gastric cancer.Methods The study included 90 specimens of left and right gastric arteries from 47 patients who underwent radical gastrectomy for carcinoma from January 2004 to 2006.All specimens were dealt with frozen section method,then they were stained with HE and immunohistochemical method respectively.Results Metastatic cancer cells were not found inside vagina vasorum in stage Ⅰ specimens.Cytokeratiu positive of tumor cens in or on vagina vasorum was showed on 25 slices from 10 tumors.Among them,2 slices from 1 tumor belonged to stage Ⅱ.8 slices from 3 tumors belonged to stage Ⅲ and 15 slices from 6 tumors belonged to stage Ⅳ.There was significant differences among cytokeratin positive rate in different stages of gastric cancer(P＜0.01).Conclusion Vaginae vasorum dissection is recommended when clearing lymph node around arteries in radical gastrectomy for carcinomas in stage Ⅱ above.

Objective To evaluate the vestibular function in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Methods Seventeen CADASIL patients were recruited in the present study and 17 healthy volunteers served as control subjects. Electronystagmogram examinations including gaze nystagmus test, spontaneous nystagmus test, saccade test, pursuit test, optokinetic nystagmus test and caloric test were performed in the subjects. Results Neither patients nor controls had gaze nystagmus or spontaneous nystagmus. There was no difference in the latency and velocity of saccade movement between patients and controls. The accuracy of the saccade movement, the accuracy in leftward saccade, was significantly lower in CADASIL group compared with controls. The pursuit movement gains was also significantly lower in CADASIL group than in control group(G_L:0.79±0.08, G_R:0.76±0.12)(t=-3.739、-2.911,P <0.05) compared with controls(G_L:0.87±0.04, G_R:0.86±0.06).The optokinetic nystagmus gains were significantly decreased in CADASIL group(G_L:0.79±0.17,G_R:0.78±0.18)(t=-2.342、-2.335,P<0.05) compared with controls(G_L:0.90±0.08,G_R:0.89±0.09). The caloric test was performed in one CADASIL patient and the result revealed an incomplete fixing inhibition. CADASIL group was further divided into normal subgroup and abnormal subgroup based on the pursuit curve. The comparison between those two subgroups demonstrated a significant correlation between the pursuit movement and the symptoms of vertigo or dizziness(P<0.05). Conclusions The central vestibular function is impaired in CADASIL patients and the abnormal vestibular function is related to the symptom of vertigo or dizziness in CADASIL patients.

AIM: To synthesize biodegradable poly lactic acid (PLA) through the optimization and selection of process and catalyst.METHODS: This experiment was performed at the Research Center for Green Chemistry and Technology in the School of Municipal and Environmental Engineering of Harbin Institute of Technology from February 2003 to October 2004. Biodegradable poly L-lactic acid (PLLA) was synthesis by melt polycondensation using L-lactic acid (LA) as material. Firstly, oligo L-lactic acid (OLLA) was prepared by dehydrating aqueous solution of LA without catalyst. And then, the mixture of OLLA and catalyst was heated at a certain temperature and pressure for a period of time to get the product of PLLA. The structure of PLLA was characterized by Fourier transform infrared (FTIR) spectra and 1H- nuclear magnetic resonance (1H-NMR) spectra. The polydispersity was determined by gel permeation chromatography (GPC).RESULTS: OLLA with the viscosity average molecular weight (Mη) of 6 500 g/mol was prepared by the following steps: the solution of LA without catalyst was dehydrated at 140 ℃, first at reduced pressure of 30 kPa for 2 hours, and then at 5 kPa for 4 hours. SnCl2-p-toluenesulfonic acid (TSA) system was the effective catalyst for the polycondensation of OLLA. After the mixture of OLLA and catalysts polycondensed at 165 ℃ and 5 kPA for about 8 hours, PLLA with the Mη of 65 000 g/mol was obtained by using SnCl2-TSA system as catalyst with the SnCl2 amount of 0.4wt% to OLLA and equal mol ratio of SnCl2 and TSA.CONCLUSION:PLLA with a certain practicability was obtained under the optimal process and catalyst. Oligomerization of LA played an important role on improving the molecular weight of PLLA.

Objective To explore the effect of applying problem-based learning (PBL) combined with ' pre-resident physician' teaching model in clinical practice in the nephrology department.Methods Sixty students in clinical practice were divided into two groups:experimental group and control group.The students in experimental group were received PBL in combination with 'pre-resident physician' teaching model while those in control group were received traditional teaching model.The effect was evaluated according to the results of theoretic examination and investigation.The results were analyzed by x2 test and P ＜ 0.05 signified statistically significant.Results The average score of theoretic examination in experimental group was 80.22 while that in control group was 76.53.The students in experimental group had significant higher score than those in control group (P ＜ 0.05 ).The overall satisfaction degree was 93.00％ in experimental group concerning the 8 indicators in PBL combined 'pre-resident physician' teaching model,while that was 73.75 ％ in control group concerning the 8 indicators in traditional teaching mode.Concerning the aspects of teaching flexibility,arousing learning interest,training literature capacity and improving ability of independent problem solving,the satisfaction degree was higher in experimental group than in control group (P ＜ 0.05 ).Conclusion PBL in combination with ' pre-resident physician' teaching model has evident advantages in clinical practice in nephrology department.

MicroRNAs (miRNAs) are a class of highly conserved small non-coding RNA molecules.They negatively regulate gene expression at the post-transcriptional level,which may lead either to translational repression or mRNA degradation.MiRNAs are highly conserved in evolution and extensively involve in organism growth,development,apoptosis,and other pathological or physiological processes.Recent studies have shown that the specific miRNAs play important roles in the process of the occurrence and development of ischemic cerebrovascular disease.At the same time,they participate in the regulation of injury mechanism as well as protection and repair mechanisms after cerebral ischemia.Fully understanding the regulatory effect of functional significance of the miRNAs and their changes of expression levels not only help identify therapeutic targets,prevent neuronal death,and reduce ischemic brain damage,but also help clear apoptotic cells,promote angiogenesis and neurogenesis by regulating their expression levels.In addition,miRNAs may also provide a new strategy for the preservation and diagnosis of ischemic stroke.