Diabetes Mellitus ; surgery ; Humans ; Kidney Transplantation ; adverse effects ; methods ; trends ; Organ Preservation ; methods ; trends ; Pancreas Transplantation ; adverse effects ; methods ; trends ; Renal Insufficiency ; surgery

Objective To explore the clinical characteristics and treatment of nodular panniculitis disease in children. Methods Clinical data of 13 cases with nodular panniculitis disease were reviewed retrospectively. Their etiology,clinical manifestation,misdiagnosis cause,pathologic characteristics, treatment and outcome were analyzed. Results Its clinical manifestation was multiform and showed mainly as fever and hypodermic nodule. Concomitant damages to digestive, respiratory, circulatory and renal system might occur in those children with the system type of this disorder. Conclusion Pediatric nodular panniculitis disease can be easily misdiagnosed and lack of specificity in the early stage, and complicates multiple organs damage.

Abdomen/surgery* ; Humans ; Laparoscopy/methods* ; Pelvic Floor/surgery* ; Perineum/surgery* ; Proctectomy ; Rectal Neoplasms/surgery*

Objective: To establish a rat model of pancr eas transplantation for basic research.Methods: The neck region of r at was selected as transplanting site and the operation was performed without cl amping the systemic circulation.The aortic segment of the graft was anastomosed with recipient's cervical artery by cuff technique,while the portal vein of the graft was anastomosed with recipient's jugular vein by microsurgical sewing.Flui d supplement and warm keeping was maintained after transplantation.Results : Seventy experiments were performed,among which 20 were former transplant ation.The total procedure of transplantation lasted about 1 hour and success rat e was 85%.The histological change was similar to those of clinical pancreas tran splantation.Conclusion: This pancreas transplantation model is simpl e and stable,while imitating the clinical procedure perfectly.It can be utilized in basic research of pancreas transplantation.

Red in vivo recombination is a new kind of genetic engineering technique based on homologous recombination. In this work, plasmid pKD46 which expresses Red recombination proteins is transferred into Escherichia coli strain DH5?.The kanamycine resistant gene is generated by PCR by using primers with homology to hisDCB gene of E.coli chromosome. Thus, the hisDCB gene was replaced with kanamycine resistant gene by the plasmid recombination system, then the resistant gene was eliminated by a helper plasmid encoding the FLP recombinase. At last, a E.coli histidine auxotroph which is sensitive to kanamycine was got. The results indicate that Red in vivo recombination is a convenient method to construct auxotrophs.

OBJECTIVETo create a gene transfer vehicle for targeting gene therapy of cancer with epidermal growth factor receptor overexpressing.

METHODSEncoding sequences of the first domain of histone gene (H1) and EGF C-loop (EGFc) were obtained by PCR amplification. These two DNA fragments were ligated by EcoR I site, and cloned and sequenced. E. coli expression vector of the fusion gene was then constructed. The fusion protein H1EGFc was purified by specific band isolation from SDS-PAGE.

RESULTSThe molecular weight of purified protein was consistent with the designed request. Its purity reached 94.02%.

CONCLUSIONA fusion protein H1EGFc was expressed and purified.

Amino Acid Sequence ; Base Sequence ; Escherichia coli ; genetics ; Gene Transfer Techniques ; Genetic Therapy ; Genetic Vectors ; Histones ; biosynthesis ; genetics ; Humans ; Molecular Sequence Data ; Protein Engineering ; Receptor, Epidermal Growth Factor ; biosynthesis ; genetics ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; isolation & purification

OBJECTIVETo establish a protocol for the targeting gene therapy against cancer with rich epidermal growth factor receptor (EGFR).

METHODSA recombinant pcDNA3.1-PE III mut was constructed and combined with a non-viral vector, a fusion protein histone H1, epidermal growth factor C-loop previously expressed by us, to be a protein-DNA complex in vitro. Using the complex to treat BT-325 and Hela cancer cells with EGFR and JK cells without EGFR. The killing rates of the cells was calculated after 48 h of incubation at 37 degrees C.

RESULTSTo BT-325 and Hela cells, the killing rates were 46.03% and 48.12% respectively. To JK cells, the complex had no killing function.

CONCLUSIONThe protocol for targeting gene therapy against cancer with EGFR has been established successfully.

ADP Ribose Transferases ; genetics ; pharmacology ; Bacterial Toxins ; genetics ; pharmacology ; Base Sequence ; Cell Line, Tumor ; Cells ; DNA ; genetics ; Exotoxins ; genetics ; pharmacology ; Gene Targeting ; Genetic Therapy ; Genetic Vectors ; Histones ; genetics ; Humans ; Molecular Sequence Data ; Receptor, Epidermal Growth Factor ; genetics ; metabolism ; Recombinant Fusion Proteins ; genetics ; metabolism ; pharmacology ; Transfection ; Virulence Factors ; genetics ; pharmacology

OBJECTIVETo investigate the effect of augmentative plate fixation to increase stability in the treatment of femoral shaft nonunions subsequent to intramedullary fixation.

METHODSNine patients with femoral nonunions after intramedullary nail internal fixation were treated with augmentative plate internal fixation from April 1998 to Jane 2008, included 8 males and 1 female, with an average age of 32 years old ranging from 21 to 54 years. One case was upper 1/3 femoral fractures, 5 cases were middle 1/3 femoral fractures, 3 cases were lower 1/3 femoral fractures. The interspace of bone nonunion was more than 5 mm in 6 cases, of them, iliac bone grafting were applied in 4 cases, artificial bone combined with iliac bone grafting were applied in 2 cases; The interspace of bone nonunion was less than 5 mm in other 3 cases,artificial bone grafting was applied in 1 case, fitting bone callus were applied in 2 cases. All patients got protected weight loading preventing the main screw break.

RESULTSAll patients achieved radiological solid union at an average of 8 months (ranged 6 to 11 months ). The fixation was removed during 6 to 11 months after operation in 5 cases. Donor site pain of iliac occurrenced on 4 cases,3 cases relieved 1 month later and 1 case relieved 3 months later. No infection, fixation loosening or breaking was observed.

CONCLUSIONThe augmentative plate fixation can be applied at the fracture site to prevent the rotational instability. The technique is simple and does not require any special instrument, which facilitates an early weight bearing and gives a quick recovery from nonunion.

Adult ; Bone Plates ; Female ; Femoral Fractures ; surgery ; Femur ; pathology ; Fracture Fixation, Intramedullary ; methods ; Fractures, Ununited ; surgery ; Humans ; Hypertrophy ; Male ; Middle Aged

OBJECTIVETo observe the effects of sodium tanshinone II A sulfonate (STS) on angiotensin II (Ang II)-induced hypertrophy of myocardial cells through the expression of phosphorylated extracellular signal-regulated kinase (p-ERK1/2).

METHODSIn the primary culture of neonatal rat myocardial cells, the total protein content in myocardial cells was determined by coomassie brilliant blue and the protein synthesis rate was measured by [3H]-Leucine incorporation as indexes for hypertrophy of myocardial cells. The expression of p-ERK1/2 was determined using Western blot and immunofluorescence labeling.

RESULTS(1) The total protein and protein synthesis rate increased significantly in contrast to the control group after the myocardial cells were stimulated by Ang II (1 micromol/L) for 24 h; STS markedly inhibited the increment of the total protein level induced by Ang II and the syntheses of protein. (2) After pretreatment of myocardial cells with Ang II (1 micromol/L) for 5 min, the p-ERK1/2 protein expression was increased, with the most obvious effect shown at about 10 min; pretreatment of myocardial cells with STS at different doses (2, 10, 50 micromol/L) for 30 min resulted in obvious inhibition of the expression of p-ERK1/2 stimulated by Ang II in a dose-dependent manner. (3) After the myocardial cells were stimulated by Ang II (1 micromol/L), the immunofluorescence of ERK1/2 rapidly appeared in the nucleus. The activation and translocation process of ERK1/2 induced by Ang II was blocked distinctly by STS.

CONCLUSIONSTS inhibited the myocardial cell hypertrophy induced by Ang II, and the mechanism may be associated with the inhibition of p-ERK1/2 expression.

Angiotensin II ; pharmacology ; Animals ; Hypertrophy ; Leucine ; metabolism ; Mitogen-Activated Protein Kinase 1 ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Myocytes, Cardiac ; drug effects ; enzymology ; pathology ; Phenanthrenes ; pharmacology ; Phosphorylation ; drug effects ; Protein Biosynthesis ; drug effects ; Protein Transport ; drug effects ; Rats ; Rats, Wistar ; Tritium

Objective To investigate the related factors of children bruxism.Methods Data of 117 children with primary and mixed dentition of 4-10 years of age were collected in the present study.There were 59 children in bruxism group and 58 children in control group.Oral and ternporomandibular joint exarninations were carried out on each child,and the parents were asked to fill the questionnaires.The data were statistically analyzed,and the relationship between the factors and the occurrence of bruxism was examined.Results The odd ratio(OR)for psychology factor,occlusal factor,specific sleep posture,parents heredity and relatives heredity were 1.074,1.528,4.472,11.164 and 8.757,respectively.Conclusions Psychology factor,occhsal factor,specific sleep posture and heredity factor are the related factors of children broxism.