1.Effects of compound Daqiqi decoction combined cisplatin on Bcl-2/Bax expression of nude mice ovarian cancer subcutaneous transplanted tumor.
Yang-bojun YANG ; Shuang WANG ; Shu LI ; Rong CHEN
China Journal of Chinese Materia Medica 2015;40(8):1575-1579
OBJECTIVETo determine the compound Daqiqi decoction( CDQD) combined cisplatin plays on the cell apoptosis of subcutaneous transplanted ovarian cancer in nude mice, to provide theory evidence for clinical treatment.
METHODMaking the models of subcutaneous transplanted ovarian cancer in nude mice, and divide the 40 mice with tumor into 5 groups (n = 8), the model control group, CDQD low dosed group, CDQD high dosed group, cisplatin group, cisplatin combined CDQD group, killing all the mice after 3 weeks' treatment and stripping tumors. Measure the volume and weight of the tumor and calculate tumor growth curve damps, the inhibition rate. Examining the expression of the Bcl-2(B-cell leukemia /lymphoma 2) and Bax (Bcl-2 associated x protein) mRNA and protein by the RT-PCR and the Western blot.
RESULT(O)The tumor weight shows that there was certain lighter effect in each CDQD group, and compared with cisplatin groups has no statistical significance, the cisplatin combined CDQD group is obviously lighter than that of the other group(P <0.01). The tumor growth curve damps shows that compared with model control group, the treatment group tumors had some extent narrowing (P < 0.01). (2) RT-PCR results: Bax (Bcl-2 associated x protein) mRNA expression shows that compared with model control group, the treatment group has increased (P < 0.01), and the cisplatin combined CDQD group compared with the other group is the highest, there was significant difference with the rest of the treatment group (P < 0.01). In the Bcl-2 mRNA express lowest in cisplatin combined CDQD group (P < 0.01), there has no difference between CDQD high dose group and cisplatin group. (Western blot shows: compared with model control group, the Bax protein of treatment group has increased expression (P < 0.01), the expression of Bax protein in cisplatin combined CDQD group is the highest(P < 0.01). There has no difference between CDQD high dose group and cisplatin group. The Bcl-2 protein expression of the cisplatin combined CDQD group is the lowest (P < 0.01), there has no difference between CDQD high dose group and cisplatin group.
CONCLUSIONThe effect of CDQD on subcutaneous transplantation ovarian tumor has promoting apoptosis function, its mechanism may be related to downgrade the Bcl-2 expression, higher expression of bax, stimulation on the apoptosis of tumor cells; cisplatin combined CDQD have synergistic effect.
Animals ; Cell Line, Tumor ; Cisplatin ; administration & dosage ; Disease Models, Animal ; Drug Therapy, Combination ; Female ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Ovarian Neoplasms ; drug therapy ; genetics ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; bcl-2-Associated X Protein ; genetics ; metabolism
2.The correlation between zinc-α2-glycoprotein and adiponectin in population with different glucose tolerance
Riu LIU ; Gangyi YANG ; Shu LI ; Ling LI ; Rong LI
Chinese Journal of Endocrinology and Metabolism 2013;(4):278-281
Objective To investigate the relationship of circulating zinc-α2-glycoprotein (ZAG) as well as adiponectin with adiposity and insulin resistance in humans.Methods Serum ZAG and adiponectin levels were determined by ELISA in 285 subjects with normal glucose tolerance (NGT),impaired glucose tolerance (IGT),and newly diagnosed cases with type 2 diabetes mellitus(T2DM).The relationships between circulating ZAG and insulin resistance or metabolic parameters were also explored.Results Circulating ZAG and adiponectin levels were all lower in T2 DM and IGT subjects than those in control subjects [ZAG:(37.14 ± 13.25 and 48.84 ± 18.74 vs 59.36 ± 16.20) mg/L,P<0.05 or P<0.01 ; adiponectin:(27.79 ± 11.23 and 33.00 ± 9.42 vs 41.81 ± 13.68) μg/L,P<0.01].Circulating ZAG was correlated positively with high density lipoprotein-cholesterol and adiponectin (all P<0.01),and inversely with body mass index,waist-hip ratio,FAT%,diastolic blood pressure,triglyceride,fasting blood glucose,fasting insulin,HbA1C,and homeostasis model assessment for insulin resistance(HOMA-IR,P<0.05 or P<0.01).By multivariate analysis,ZAG levels were independently associated with body mass index,HOMA-IR,diastolic blood pressure,and adiponectin (P<0.05 or P<0.01).Conclusions ZAG levels,associated with fat,insulin sensitivity,and adiponectin expression,are likely to play an important role in insulin resistance and energy homeostasis in humans.
3.To discuss that language art's utilize in building harmonious relation of nurse and patient
Chun-Hui LIU ; Gui-Rong YANG ; Meng-Shu WU
Chinese Medical Ethics 1994;0(05):-
To promote quality of the whole nursing,build harmonious relation of nurse and patient is basement.Nurse how to make language art utilize at the interflow of nurse and patient,to promote understanding and sustain between nurse and patient,improve cure,nursing effect,reduce contradictory of nurse and patient is the key of building harmonious relation of nurse and patient.
4.Clinical study and acoustical analysis for submucosa hemorrhage of vocal cords treated mainly with acupuncture at "Kaiyin point no. 1".
Shu-rong YANG ; Qiang XIE ; Juan-juan JIANG
Chinese Acupuncture & Moxibustion 2006;26(4):243-246
OBJECTIVETo evaluate therapeutic effect of acupuncture at "Kaiyin point No. 1" on submucosal hemorrage of vocal cords.
METHODSOne hundred and sixty cases were randomly divided into a test group and a control group. The test group were treated by acupuncture at "Kaiyin point No. 1" with reducing method in reinforcing or reducing method by manipulating the needle in cooperation with the patient's respiration, combined with deep respiratory movement of the glottis laryngeal cavity, once daily; and the control group by spray inhalation of western medicine, once daily, 7 days constituting one therapeutic course.
RESULTSThe cured and markedly effective rate and the total effective rate were 85.0% and 97.5% in the test group and 60.0% and 85.0% in the control group respectively, with a significant difference between the two groups. After treatment of the test group,the acoustical parameters maximum phonation time (MPT), frequency perturbation quotient (FPQ), amplitude perturbation quotient (APQ), ratio of harmonic to noise (H/N) objectively reflected improvement of voice quality.
CONCLUSIONAcupuncture at "Kaiyin point No. 1" as main method is an ideal therapy for submucosa hemorrhage of vocal cords.
Acoustics ; Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Hemorrhage ; physiopathology ; therapy ; Humans ; Laryngeal Diseases ; physiopathology ; therapy ; Male ; Middle Aged ; Vocal Cords ; physiopathology
5.Clear cell meningioma: report of a case.
Zhi-yi ZHOU ; Rong-chao SUN ; Shu-dong YANG ; Jia-bei LIANG ; Jun RUI
Chinese Journal of Pathology 2009;38(8):562-563
Diagnosis, Differential
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Ependymoma
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metabolism
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pathology
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Female
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Hemangioblastoma
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metabolism
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pathology
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Humans
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Ki-67 Antigen
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metabolism
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Meningeal Neoplasms
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metabolism
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pathology
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surgery
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Meningioma
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metabolism
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pathology
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surgery
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Middle Aged
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Mucin-1
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metabolism
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Neoplasm Recurrence, Local
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Vimentin
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metabolism
6.Icariin Promoted Osteogenic Differentiation of SD Rat Bone Marrow Mesenchymal Stem Cells: an Experimental Study.
Shu-ping FU ; Li YANG ; Hao HONG ; Chen OU ; Rong-hua ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(7):839-846
OBJECTIVETo observe features of Icariin in promoting osteogenic differentiation of SD rat bone marrow mesenchymal stem cells (BMSCs) in vitro.
METHODS(1) SD rats' BMSCs were isolated and purified by mechanically isolated and cultured by whole bone marrow adherent method. Effects of various concentrations Icariin on serum activities of alkaline phosphatase (ALP) were detected using amino antipyrine phenol determination method at day 3, 6, 9, 12, 15, 18, and 21. Calcium nodes of each groups were detected using alizarin red staining. Roles of various concentrations Icariin in promoting osteogenic differentiation of BMSCs were observed. (2) BMSCs were divided into the blank control group, the osteogenic induced group, and the Icariin group (0.5 microg/mL). ALP activities were detected at day 7, 14, and 21 of culture. Meanwhile, ALP positive staining rate and calcium nodes were detected at day 14 and 21 respectively. Additionally, mRNA expressions of Runt-related transcription factor-2 (Runx2) and Osteocalcin were detected at day 7, 14, and 21 by real-time fluorescent quantitative PCR.
RESULTS(1) 0.05-5.0 microg/mL Icariin could significantly elevate serum ALP activities. Of them, 0.2-2.0 microg/mL Icariin significantly increased calcium nodes numbers (P < 0.01). (2) When Icariin promoted osteogenic differentiation of BMSCs, Runx2 mRNA expression levels and ALP activities increased earlier and then decreased, while osteocalcin mRNA expression levels continued to increase (P < 0.01). Compared with the osteogenic induced group, ALP activities and ALP positive staining rate were both elevated after 14 days of Icariin treatment in the Icariin group (P < 0.05, P < 0.01).
CONCLUSIONSIcariin could promote the differentiation of BMSCs to osteoblasts by up-regulating Runx2 mRNA expression levels. It also could promote the mineralization by increasing ALP secretion and Osteocalcin mRNA expression levels, thereby promoting mature of newly generated osteoblasts.
Animals ; Bone Marrow Cells ; Cell Differentiation ; drug effects ; Flavonoids ; pharmacology ; Hematopoietic Stem Cells ; Mesenchymal Stromal Cells ; physiology ; Osteoblasts ; Osteocalcin ; Osteogenesis ; Rats ; Rats, Sprague-Dawley
7.The morbidity of pneumoconiosis in Jiaozuo city.
Zhen-rong MIAO ; Xiao-fa YANG ; Lian-xia GUO ; Shu-xia SHI ; Fang-li DU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):298-299
Adult
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China
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epidemiology
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Coal Mining
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Humans
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Middle Aged
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Morbidity
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Pneumoconiosis
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epidemiology
8.The effects of semiconductor laser irradiation and a high voltage static electric field on small vessel impairment in diabetic rats
Min GAO ; Li-Rong AN ; Yang SUN ; Zheng LV ; Shu-Fang SUN ; Hui-Juan CHEN ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(04):-
Objective To study the effects of semiconductor laser irradiation and high voltage static electric fields on small vessel damage in diabetic rats.Methods Fifty healthy male Wistar rats were randomly divided into two groups:8 rats in a normal group and 42 in a diabetic model group.The diabetic models were created by intrape- ritoneal injection of streptozocin.The diabetic model rats were randomly divided into four subgroups:a diabetes group,a semiconductor laser treatment group,a high voltage static electric field treatment group and a comprehensive treatment group receiving combined semiconductor laser and high voltage static electric field exposure.The rats in each treatment group were subjected to the corresponding intervention.After 20 days of treatment,the venous blood, kidney tissue and myocardium tissue of the rats were collected,and the concentrations of blood glucose,insulin,en- dothelin and tissue were detected.Results Compared with the normal group,a significant increase in blood glu- cose and endothelin was observed in the diabetic model group,along with significantly decreased blood insulin and significant small vessel endothelium proliferation in the kidney tissue.Compared with the diabetes group,endothelin levels were significantly lower in all 3 treatment groups,and blood insulin was also higher in the comprehensive treat- ment group.Conclusion There were severe abnormalities in blood glucose,insulin and endothelin as well as mild impairment of small vessel endothelium proliferation in the diabetic rats.Semiconductor laser and high voltage static electric field exposure have a role in treating and preventing these conditions in diabetic rats.
9.A Rapid Screening Model for Biosurfactant Producing Strains
Chang-Fa NING ; Wei SHEN ; Guang-Rong MENG ; Shu-Lin YANG ;
Microbiology 1992;0(03):-
A rapid screening model for biosurfactant-producing strains is described in this paper, according as biosurfac-tant can make the blood-plate hemolysis and produce the blue cycles in blue-glue-plate. 12 microorganisms have been got from soil and water samples sampled from oil field and refinery using this model, and one of them can produce the glyco-lipid biosurfactant, which can depress the interfacial tension of water from 71. 3mN/m to 30. 5mN/m, and yield is 6.5 g/L.
10.Survey on the Current Situation and Thinking on the Reform of the Basic Medicine Experiment in Higher Vocational Teaching Education
Rong LONG ; Yang SHU ; Bin SU ; Lan LIU ; Mingwu SONG ; Binxue LI
Chinese Journal of Medical Education Research 2003;0(03):-
The basic medicine experiment plays a very significant role in training medical students'creative thinking and working ability.Meantime,it also plays an important role in understanding and exploiting related medical theories.It is the main access to the guarantee of experimental teaching quality to open the laboratory,to establish original experiments and to employ various academic attainments' assessment.