Objective The study is to investigate the effect of tanshinone on lung reperfusion injury. Methods 24 New-Zealand rabbits were randomly divided into two groups. In one group, the lungs were perfused and preserved with LPD solution, the other with LPD+ tanshinone solution. The isolated lungs were preserved for 18 hours and then reperfused with rewarming whole blood. After reperfusing 30 minutes the lung samples were examined by pathology, wet/dry weight and measure of NO, SOD and MDA. Results In two groups, the reperfusion injury lungs were fresh, but had partial congestion, edema and ecchymoma under naked eye, under light microscopy the structures of the pulmonary vesiculars, branchia and capillaries were complete, but the epithelial cells of the vesicles and capillaries in the LPD group were more swelling and hypertrophy than that in LPD+ tanshinone group. The wet/dry weight ratio was significantly lower in LPD+ tanshinone group than in the LPD group. NO [(2.16±2.15)μmol/g] and SOD [(37.03±18.01NU/mg] were higher in the LPD+ tanshinone group than in the LPD group [(0.68±0.33) μmol/g, (17.63±11.04)NU/mg] (all P<0.05), MDA was lower in the LPD+ tanshinone group [(0.47±0.20)μmol/mg] than in the LPD group [(0.86±0.44)μmol/mg] (P<0.05). Conclusions Tanshinone can dean oxygen free radical and is of stronger antioxidation, results in alleriating of lung reperfusing injury. Tanshinone has positive effect on preservative quality of the reperfusion injury lung.

Clinical Medicine ; Humans ; Neoplasms ; prevention & control ; Periodicals as Topic ; Research

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature

Acetic Acid ; Biopsy ; Eosine Yellowish-(YS) ; Fixatives ; Formaldehyde ; Hematoxylin ; Humans ; Infertility, Male ; pathology ; Male ; Picrates ; Spermatozoa ; pathology ; Testis ; pathology ; Tissue Fixation ; methods

Based on their multiple years of personal experiences in hospital administration,authors of this article point out that medical service,humanistic service,and emotional service should be the core aspects in the current hospital competition.Medical professionalism embodied in the three levels of consciousness,behaviors,and regulations should be the main subject in the research on the role of medical ethics in hospital administration and medical treatment behaviors.It is an urgent ethical requirement and the chief task for hospital administrators to achieve humanistic medical service through humanistic administration of hospitals.

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Carbon Monoxide Poisoning ; epidemiology ; therapy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Inpatients ; statistics & numerical data ; Male ; Middle Aged ; Young Adult

Objective To study the influence of valproate sodium(VPA)on neuroprotective effects of topiramate(TPM).Methods For-ty-eight 3-4 week-old male Wistar rats were randomly divided into 4 groups of 12 rats each.Group A was negative control rats,and groups B-D were rat model of epilepsy,induced by pentylenetetrazol(PTZ).The rats in 2 experimental groups were adminstered intragastrically with TPM 40 mg/kg and TPM 40 mg/kg+ VPA 200 mg/kg;2 control groups(groups A and B)with the equal amount of distilled water administration.After 2-month administration,changes of the behavior,levels of serum neuron-specific enolase(NSE)and the pathological in hippocampus were examined.Results The level of NSE in the group of TPM were significantly lower than that in masculine group,but no difference between masculine group and the TPM plus VPA.The pathological change in hippocampus were abatement in the group of TPM.Conclusion TPM plus high dosage of VPA will impaire the neuroprotective effects of TPM.

Objective To explore the effect of brain derived neurotrophic factor(BDNF) on the birth weight and the brain weight of newborn rats with intrauterine growth retardation(IUGR).Methods Female and male Sprague-Dawley rats were mated together and the vaginal plug formation after mating was for pregnancy diagnosis.The day when the plug was observed was considered day 1 of gestation.Then,the pregnant rats were divided into IUGR group and sham surgery group.On day 17 of gestation,the uterine arteries of pregnant rats in IUGR group were clamped for 30 min by blood vessel forceps after abdominal cavity with clamping blood uessels was opened.The pegnant rats in sham surgery group only had abdominal cavity opened without clamping blood vessels.Then the pegnant rats in IUGR group received 9 g/L saline(IUGR group) or BDNF 1 ?g injected in the rat through caudal veins for 5 days after surgery(BDNF intervention group).After vaginal birth,the weight of newborn rats and their brain weigh were measured and analyzed.Results Compared with the sham surgery group,the birth weight and brain weight of IUGR group group rats decreased by 24.9% and 17.3%,respectively,the results were significant(Pa

Acupuncture Points ; Head ; anatomy & histology ; Humans

Catheter Ablation ; Consensus Development Conferences as Topic ; Humans ; Microsurgery ; Neoplasms ; therapy ; Radiotherapy Planning, Computer-Assisted ; Radiotherapy, Intensity-Modulated