Adult ; Enzyme-Linked Immunosorbent Assay ; Female ; HSP70 Heat-Shock Proteins ; blood ; Humans ; Lead ; toxicity ; Male ; Occupational Exposure

Metastatic epidural compression of the spinal cord is a significant source of morbidity in patients with systemic cancer. With improvment of oncotheray, survival period in the patients is improving and metastatic cord compression is en- countered increasingly often. Surgical management performed for early circumferential decompression for the spinal cord com- pression with spine instability, and spine reconstruction performed. Patients with radiosensitive tumours without spine instabili- ty, radiotherapy is an effective therapy. Spinal stereotactic radiosurgery and minimally invasive techniques, such as vertebro- plasty and kyphoplasty, percutaneous pedicle screw fixation, radiofrequency ablation are promising options for treatment of cer- tain selected patients with spinal metastases.
Decompression, Surgical ; Humans ; Minimally Invasive Surgical Procedures ; Spinal Cord Compression ; therapy ; Spinal Neoplasms ; secondary ; therapy

Objective To evaluate endoluminal repair and preoperative management for acute Standford type B aortic dissection complicating massive hydrothorax. Methods The clinical data of 27 patients (23 males, 4 females) hospitalized from January 2003 to December 2008 were analysed retrospectively. The average age was 47 ±9 years (35 ～70). Eleven patients had bilateral huge hydrothorax (40. 7% ) , while 13 had left hydrothorax (48. 1% ) and 3 had right hydrothorax (11. 1% ) only, and in 2 of them with additional pericardial effusion (7.4% ). SaO2 was below 90% in all cases. All patients underwent emergency endovascular repair. For control of massive hydrothorax found by CT or chest fluoroscopy, puncture drainage or tube drainage were administrated postoperatively. Results All the 27 operations were successful, and there was no perioperative mortality. Three type Ⅰ and one type Ⅳ endoleaks occurred but disappeared in one month. Hydrothorax disappeared 28 days to 3 months postoperatively in all patients, of which 5 cases had puncture drainage (18.5%) and 1 case had tube drainage ( 3. 7% ). Mean follow-up was 30 ± 20 m ( 6 ～ 78 m ) after endovascular management. Complications included pleural thickening (6 of 27, 22. 2% ) , pulmonary atelectasis (2 of 27, 7. 4% ) , and lung consolidation combined with chest dent (2 of 27, 7. 4% ). Conclusions Emergency endovascular therapy is safe and effective for acute Stanford type B aortic dissection with massive hydrothorax. Drainage of hydrothorax after stent-graft deployment is a must for the patient suffering from severe respiratory failure.

AIM:TostudythechangeofradiosensitivityofU251cellsaftertreatedwithsodiumdichloroacetate ( DCA) and further to explore the possible mechanism .METHODS: The U251 cells were divided into 4 groups: control group, DCA group, X-ray irradiation without DCA pretreatment ( IR) group and X-ray irradiation with DCA pretreatment ( DIR) group.MTT assay was applied to determine the cell viability .The intracellular reactive oxygen species ( ROS) were detected by DHE fluorescence .The expression level of Bcl-2 was assessed by Western blot .The percentage of apoptosis of cells was determined by flow cytometry .RESULTS:No difference between control group and DCA group in cell viability (P>0.05) was observed.However, the cell viability of both IR group and DIR group was markedly reduced compared with control group ( P<0.05).Furthermore, the viability of DIR group was significantly decreased compared with IR group ( P<0.05 ) .The percentage of ROS positive cells was obviously increased in DIR group compared with IR group (P<0.05).The expression level of Bcl-2 was sharply decreased in DIR group (P<0.05) and the percentage of apoptosis of cells was significantly elevated ( P<0.05) in DIR group compared with IR group .CONCLUSION:The better antitu-mor effect was obtained by improving the radiosensitivity through pretreating the cells with DCA , and the possible mecha-nism was down-regulation of the Bcl-2 expression by developing the intracellular ROS .

Objective To explore the surgical management for arterial diseases. Methods The clinical data of 86 cases of arterial diseases were analysed retrospectively. These cases included:3 cases of thoracic aortic aneurysm,2 cases of descending aortic dissection with rupture and aneurysm formation,2 cases of abdominal aortic dissection with false aneurysm,1 case of abdominal aortic aneurysm(AAA)with duodenojejunal fistula and GI bleeding,5 cases of ruptured AAA with shock, 3 cases of traumatic rupture of abdominal aorta,21 cases of AAA combined with unilateral or bilateral iliac aneurysms,6 cases of iliac aneurysms,9 cases of popliteal aneurysms,21 cases of false aneurysm of iliac or popliteal artery,1 case of rupture of junction of subclavian and vertebral artery with false aneurysm,3 cases of rupture of subclavian artery with false aneurysm,2 cases of aneurysm of carotid artery and 7 cases of false aneurysm of carotid artery. Prosthetic vascular graft was performed in 71(cases),autologous vein graft in 3 cases and suture(repair) of arterial rupture in 12 cases.Results Operative (mortalty) was 3.5%(3/86)in this series.Seventy-one cases were followed-up for 1 month to 5 years, the results showed that all the patients were alive and well.Conclusions In the(management) of(appropriate) aortic diseases, the use of endoluminal technique is simple, microtraumtic, safe and has few complications. It also can shorten the hospital stay of the patient. Endovascular technique should be the first choice for patients with aortic diseases.

70 bpm during scan),the proportion of segments that could not be assessed because of motion artifact were 0.1%(1/759),1.1%(7/649),2.5% (10/407),42.6%(103/242),and 75.5%(108/143),respectively.With conventional selective coronary angiography as the golden standard,the sensitivity,specificity,positive and negative prediction values to detect≥50% stenotic lesions in the assessable segments were 79.2%,96.0%,83.8%,and 94.6%,respectively.There was a significant correlation between the number of segments per patient not assessable because of motion artifact and heart rate during the scan(r=0.655,P=0.000).Conclusion MSCT is capable of achieving high accuracy for detection of coronary artery stenosis,and is a reliable technique to diagnose coronary artery disease.

Objective To explore the application of diffusion weighted imaging(DWI) in diagnosis of viral encephalitis in children.Methods Conventional MR imaging(T2WI and T1WI) and DWI were performed on 20 patients with viral encephalitis diagnosed clinically.Location and number of lesions demonstrated on these imagings and percents in their abnormality were compared.Results Percen-(tage of abnormality) demonstrated on DWI was significantly higher than that on T1WI(?~2=4.44 P

Objective To detect the level of serum cystatin C(CysC),macrophage migration inhibitory factor(MIF),urinary podocytes(UPC) and explore the correlation between the levels of the three and therapeutic effect in children with primary nephrotic syndrome(PNS).Methods The serum CysC,MIF levels were detected in 38 children with PNS,which were treated before and after remission by double-antibody sandwich sensitive enzyme-linked immunosorbent assay(ELISA).Podocyte-specific marker protein-Podocalyxin(PCX) was detected by immunofluorescene to definite UPC before and after remission and compare with those of healthy control group(n=15).Results Compared with control group,the levels of serum MIF were significantly increased in 22 patients responded sensitively to prednisone prior and post treatment(Pa0.05),however,CysC,MIF and UPC significantly increased after treatment in 16 steroid-resistant children(Pa0.05), MIF significantly decreased(Pa0.05)in steroid-resistant children after treatment.In 16 children with steroid-resistant, complete remission of PNS as cyclophosphamide pulse treatment being added in 10 patients,which CysC, MIF,UPC were significantly higher than those in control group(Pa

Objective To explore the expression of vascular endothelial growth factor(VEGF) and macrophage migration inhibitory factor(MIF) in kidney of diabetic rats,the influence of protein kinase C ? inhibitor LY333531 on the expression of VEGF and MIF.Methods Thirty wistar rats were divided at random into three groups:normal control group(NC group);diabetic model group(DM group),the rats models of diabetic mellitus were induced by streptozotocin(SIZ);LY333531 treated group(LT group),which were given LY333531 [10 mg/(kg?d)] by gastric perfusion after DM model inducing success.At the 10th week,the levels of blood glucose(BG),24 hours urine albumin excretion rate(AER),urine VEGF,and MIF were detected using enzyme-linked immunosorbent assay(ELISA).Glomerular cellularity and extracellular matrix(ECM) were observed by light microscope.The expression levels of VEGF and MIF in the kidney tissues in three groups of rats were detected using immunohistochemistry.Results BG,AER,VEGF,MIF in DM group were significantly higher than those in control group(Pa0.05).There was strongly stained of VEGF,MIF in glomerular mesangium in DM group,weakly positive in LT group by immunohistochemistry.The expression of VEGF and MIF in DM gorup significantly increased than those in NC group(P0.05).Conclusions The expression levels of VEGF and MIF in kidney tisues of diabetic rats significantly increase and correlation with the degree of renal tissue injury.LY333531 can contribute to cut down BG,decrease AER,VEGF,MIF excrection in urine,inhibit glomerular cellularity and ECM proliferation,inhibit expressions of VEGF and MIF to protect kidney.

Objective To explore the effect of 1,25-(OH)2D3 on Nephrin protein expression in renal of diabetic rats.Methods Diabetic rats were induced by streptozotocin(STZ).Groups were divided as follows:diabetic group(DM group),1,25-(OH)2D3 treated group[1,25-(OH)2D3 group] and control group.At the 12th week,haematuria and proteinuria were determined and the kidneys were removed by histopathology,immunostaining,and the expression of Nephrin mRNA and protein in renal cortex were detected by reversed transcription-polymerase chain reaction(RT-PCR).Results Compared with control and 1,25-(OH)2D3 groups,urine erythrocyte and protein excretion and glomerlar cellularity in DM group statistically increased(P0.05).Conclusion The effect of 1,25-(OH)2D3 is in relieving haematuria and proteinuria and up-regulate the expression of nephrin mRNA and protein to protein kidney.