1.Gastric collision tumor (adenocarcinoma and gastrointestinal stromal tumor): report of a case.
Jian WU ; Yu-jie HE ; Shu-lan TONG
Chinese Journal of Pathology 2010;39(8):563-564
Adenocarcinoma
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metabolism
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pathology
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surgery
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Aged
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Female
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Gastrectomy
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methods
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Gastrointestinal Stromal Tumors
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metabolism
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pathology
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surgery
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Humans
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Keratins
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metabolism
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Neoplasms, Multiple Primary
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metabolism
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pathology
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surgery
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Proto-Oncogene Proteins c-kit
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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surgery
2.Gefitinib for advanced bronchioloalveolar carcinoma.
Xiao-tong ZHANG ; Shu-lan WANG ; Long-yun LI
Acta Academiae Medicinae Sinicae 2004;26(3):340-343
We described 3 cases of advanced bronchioloalveolar carcinoma (BAC), in whom once-daily treatment with 250 mg Gefitinib (Iressa) demonstrated remarkable antitumor effects. Gefitinib may produce dramatic clinical responses when administered to patients with poor performance status who had received heavy platinum/docetaxel-based prior chemotherapy.
Adenocarcinoma, Bronchiolo-Alveolar
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drug therapy
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Aged
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Antineoplastic Agents
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therapeutic use
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Female
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Humans
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Lung Neoplasms
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drug therapy
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Middle Aged
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Quinazolines
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therapeutic use
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Receptor, Epidermal Growth Factor
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antagonists & inhibitors
3.The expression and significance of glucose-6-phosphate isomerase mRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Guan-Fei ZHAO ; Yong-Zhe LI ; Yu-Guo SONG ; Chao-Jun HU ; Da-Wei TONG ; Shu-Lan ZHANG ;
Chinese Journal of Rheumatology 2001;0(04):-
Objective To investigate the mRNA level of glucose-6-phosphate isomerase(GPI)ex- pression in patients with rheumatoid arthritis(RA).Method Reverse transcription-polymerase chain reaction (RT-PCR)was applied to semiquantitatively analyze GPI mRNA expression in the peripheral blood mononu- clear cells(PBMCs)of 30 active RA patients,30 stable RA patients,30 patients with other rheumatic disease and 30 healthy subjects.Results There was statistically significant differences between patients with RA and other rheumatic diseases(P
4.Clinical analysis of 12 cases of acute myeloid leukemia with Ph chromosome and BCR-ABL positive.
Xin-Hong FEI ; Shu-Lan WU ; Rui-Juan SUN ; Jia-Rui ZHOU ; Jing-Bo WANG ; Tong WANG ; Hong-Xing LIU ; Hui WANG ; Chun-Rong TONG ; Tong WU ; Dao-Pei LU
Journal of Experimental Hematology 2012;20(3):545-548
This study was purposed to analyze the characteristics of morphology, immunology, cytogenetic and molecular biology of leukemia cells in 12 AML patients with Ph(+) and their correlation with survival of patients. 12 patients with Ph(+) AML were diagnosed according to diagnostic criteria of WHO and existence of t(9;22) (q34;q11) or t(9;22) abnormality, meanwhile no evidence of CML chronic phase was observed. The results showed that 8 out of 12 cases were confirmedly diagnosed to be AML by morphologic and immunophenotypic examination, 4 cases were diagnosed as myeloid and B lymphocytic mixed acute leukemia. The Ph chromosome was detected in 10 cases by chromosome analysis at the first time of diagnosis, and some of the cases had coexistence of complex chromosome and/or normal karyotype. BCR-ABL transcript was detected in all 12 cases, including 7 cases with b3a2, 1 case with b2a2, 1 case with b2a2 variants, 2 cases with e1a2 and 1 case with e18a2. The 12 cases all got complete remission after chemotherapy and/or gleevec treatment, out of them 3 cases received chemotherapy and gleevec treatment, but 2 cases died; 9 cases received allogeneic hematopoietic stem-cell transplantation (allo-HSCT), 1 case died from relapse, among them 1 case died from transplant complications. The median survival was 24 (8 - 80) months, the overall survival of 3 years was (51.4 ± 17.7)%. It is concluded that the Ph(+) AML is a acute myelogenous leukemia with poor prognosis, but long-term survival may be achieved with HSCT as quick as after complete remission from gleevec and chemotherapy treatment. Meanwhile, the detection of BCR-ABL gene and it variants may be give more opportunity for diagnose and treatment, which can be used as routine screening for newly diagnosed leukemia.
Adult
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Child
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Female
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Hematopoietic Stem Cell Transplantation
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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diagnosis
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Male
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Middle Aged
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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diagnosis
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Prognosis
5.Short-term outcome of the acellular dermal matrix in dental implant surgical sites.
Shu-lan XU ; Lei ZHOU ; Shi-tong XU ; Wei-ping LIU ; Wang-xi WU ; Chun-xian WANG
Chinese Journal of Stomatology 2010;45(7):435-438
OBJECTIVETo evaluate the clinical short-term results of the acellular dermal matrix for guided bone regeneration.
METHODSSixty-four patients with bone defect in anterior maxillary area (average bone width: 3 mm) were included. Ridge-splitting technique with simultaneous placement of implants and artificial bone material implantation was performed in 21 patients (non-membrane group). Forty-three patients received the same procedure but with acellular dermal matrix covering the surgical sites (membrane group). The patients were followed up for three months and the new bone formation was checked in clinic and by X-ray.
RESULTSThree months after operation, the membrane group showed good osseointegration and high bone density over the implant cover screws. In the second operation, the membranes became thinner and the new bone fully covered the implant in the membrane group. The labial bone exhibited slight absorption and labial surface of 7 implants in 7 patients was exposed in non-membrane group. The width and the height of the ridge in the second operation were greater in membrane group than in non-membrane group (P < 0.05).
CONCLUSIONSThe acellular dermal matrix can effectively resist the growth of soft tissue to allow bone regeneration around the implant.
Acellular Dermis ; Bone Regeneration ; Bone Substitutes ; Dental Implants ; Guided Tissue Regeneration, Periodontal ; Humans
6.Clinical application of maxillary sinus lift with Summers osteotome.
Lei ZHOU ; Shu-lan XU ; Shi-tong XU ; Jian-sheng HUANG ; Guang-bao SONG ; Xue-yang ZHANG
West China Journal of Stomatology 2008;26(3):296-298
OBJECTIVETo introduce and evaluate the procedure and the effect of maxillary sinus lift with closed technique by Summers osteotome, bone grafting and simultaneous implant placement.
METHODS66 cases with severely resorbed alveolar bone in maxillary posterior region received sinus lift with Summers osteotome, simultaneously bone grafting and implants placement. The final restoration was finished at 6 months postoperatively.
RESULTSThe operation procedure were eventless in the 66 cases, the sinus floor were elevated by 2-5 mm, three-dimensional reconstruction of the CT scan pictures showed the smooth dome profile of the lifting sites and no signs of laceration on the membrane, and there were no maxillary antritis after operation. After 6 months, no significantly bone graft resorption and good osseointegration were noticed in X-ray imaging. The final restoration was finished at this time. 12-24 months after the restoration, all implants inserted were remain, the hard and soft tissue were healthy, prosthesis were stable and functioned. X-ray showed good osseointegration in the lifting sites, the vertical resorption around the implants were less than 1 mm.
CONCLUSIONWith properly use of Summers osteotome, scraps of the bone in the implant sockets can be pushed into the sinus, these autogenous bone scraps were in favor of the osseogenesis and the sinus floor can be easily elevated by the method with very infrequent complications. It enlarged indication of dental implants and avoided operation of harvesting autogenous bone in other site. The method is simple and valuable to clinical application.
Adult ; Alveolar Bone Loss ; Bone Transplantation ; Dental Implantation, Endosseous ; Dental Implants ; Humans ; Male ; Maxilla ; Maxillary Sinus ; Middle Aged ; Osseointegration ; Osteotomy ; Sinus Floor Augmentation
7.Comparison of shade matching accuracy of tabs of shadeguide and tabs out of shadeguide.
Ming-Ming XU ; Tong-Kai XU ; Feng LIU ; Shu-Xin REN ; Hai-Lan FENG
Chinese Journal of Stomatology 2009;44(7):430-432
OBJECTIVETo compare the shade matching accuracy between tabs of shade guide and tabs out of shade guide by prosthodontists.
METHODSSixty-two graduate students who had 1 to 5 years clinical experience without color blindness were included in this study. Twenty-nine tabs from Vita 3D-Master shadeguide and 7 tabs out of Vita 3D-Master shade guide but from Vita Bleachedguide 3D-Master were taken as the targets of shade matching and observed under the standard light. Subjects' shade matching accuracy on tabs of shadeguide and tabs out of shadeguide was recorded. Frequency of right match was analyzed using Monte Carlo exact probability-test. Wilcoxin test was used to analyze the difference between each group on ranked data of value, chroma and hue.
RESULTSThe shade matching accuracy of tabs of shade guide [45.9% (199/434)] was significantly higher than that of tabs out of shade guide [9.0% (39/434)]. There was significant difference between the two kinds of tabs of ranked data of value [0.2 (0.1 - 0.3), 0.4 (0.4 - 0.5)] and chroma [0.2 (0.1 - 0.4), 0.5 (0.4 - 0.6), P < 0.01, but there was no significant difference in hue data [0.3 (0.1 - 0.4), 0.3 (0.1 - 0.4)].
CONCLUSIONSThe shade matching ability of prosthodontists on tabs out of shade guide is lower than that on tabs of shadeguide. To fulfill clinical need, more training is necessary.
Dentists ; psychology ; Esthetics, Dental ; Humans ; Observer Variation ; Pattern Recognition, Visual ; Prosthesis Coloring ; methods ; Reproducibility of Results
8.Effects of purinergic analogues on spontaneous contraction and electrical activities of gastric antral circular muscle in guinea-pig.
Zheng JIN ; Hui-Shu GUO ; Dong-Yuan XU ; Ming-Yu HONG ; Xiang-Lan LI ; Wen-Xie XU
Acta Physiologica Sinica 2004;56(6):678-684
In order to investigate the role of non-adrenergic non-cholinergic nerves in regulating mechanical and electrical activity of gastric circular smooth muscle, the effects of ATP and its analogues on gastric motility and electrical activities were observed in guinea-pig. In organ bath system, isometric force of the circular smooth muscle of guinea-pig gastric antrum was measured. Electrical activity of the muscle was recorded by using intracellular microelectrode. Electrical and mechanical activities were recorded by chart recorder. ATP and 2-MeSATP potentiated the mechanical activity but did not affect electrical activity in gastric circular smooth muscle. ATP and 2-MeSATP-induced contraction was effectively blocked by nonselective P2y-purinoceptor antagonist, reactive-blue-2 and suramin, but ATP-induced contraction was not blocked by alpha,beta-MeATP-induced desensitization of P2x-purinoceptors. However, alpha,beta-MeATP, P2x-purinoceptor agonist, attenuated slow waves with membrane hyperpolarization and inhibited contraction. The relaxation by beta,gamma-MeATP was blocked by alpha,beta-MeATP-induced desensitization of P2x-purinoceptors. ATP-induced contraction was blocked by external calcium-free, but not by nicardipine, a L-type calcium channel blocker. Indomethacin, a nonselective cyclooxygenase inhibitor, did not block ATP-induced contraction. The results suggest that: (1) ATP- and analogues-induced contraction is mediated by P2y-purinoceptor, whereas alpha,beta-MeATP-induced relaxation by P2x-purinoceptor in guinea-pig gastric antral circular smooth muscle. (2) ATP-induced contraction is dependent on extracellular calcium, but Ca2+ entry is not mediated by L-type calcium channel. (3) Prostaglandins are not involved in ATP- and analogue-induced contraction of gastric circular smooth muscle in guinea-pigs, and alpha,beta-MeATP-induced relaxation is related to membrane hyperpolarization.
Adenosine Triphosphate
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analogs & derivatives
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pharmacology
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Animals
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Electrophysiology
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Guinea Pigs
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In Vitro Techniques
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Microelectrodes
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Muscle Contraction
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drug effects
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physiology
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Muscle, Smooth
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drug effects
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physiology
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Purinergic Agonists
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Pyloric Antrum
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drug effects
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physiology
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Thionucleotides
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pharmacology
9.Study of molecular mechanism of tanshinone II A inducing differentiation in acute promyelocytic leukemia NB4 cells.
Rui DU ; Hong ZHENG ; Yan-Ping WANG ; Wen-Tong MENG ; Hui QIN ; Shu-Lan YUAN
China Journal of Chinese Materia Medica 2008;33(24):2954-2958
OBJECTIVETo investigate molecular mechanism of tanshinone II A inducing differentiation and apoptosis in acute promyelocytic leukemia NB4 cells.
METHODNB4 cells were cultured in vitro and treated with tanshinone II A and observed cellular morphology, cell category and the cellular proliferation. DNA microarray technique was used to analyze the gene expression profiles of NB4 cells induced by tanshinone II A.
RESULT92.8% of NB4 cells treated with 0.5 mg x L(-1) tanshinone II A were induced into mature neutrophils, in which myetocytes and melamyetocytes were 27.0%, banded and segmented neutrophits 68.2%. Cell growth were inhibited. cDNA microarray showed the enormously expressed 183 genes including 23 differentiation associated genes, and other interrelated genes.
CONCLUSIONTanshinone II A inducing differentiation in NB4 cells may be via regulation of many kinds of genes, especially differentiation associated genes expression. This partially explained the molecular mechanism of tanshinone II A inducing differentiation.
Cell Differentiation ; drug effects ; Cell Line, Tumor ; Diterpenes, Abietane ; Drugs, Chinese Herbal ; pharmacology ; Gene Expression Regulation, Leukemic ; drug effects ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; genetics ; metabolism ; Phenanthrenes ; pharmacology
10.The comparison of the three anti-dsDNA antibody detecting test.
Da-Wei TONG ; Shu-Lan ZHANG ; Yong-Zhe LI ; Chao-Jun HU
Chinese Journal of Experimental and Clinical Virology 2009;23(1):74-75
OBJECTIVETo compare the three Anti-dsDNA antibody detecting test (IIF, ELISA, Farr) with 200 serum samples to evaluate which one has higher sensitivity and specificity.
METHODS200 serum samples including 120 serum samples of SLE, 20 serum samples of rheumatoid arthritis, 20 serum samples of MCTD, 20 serum samples of SS, 20 serum samples of PSS and 50 serum samples of healthy measured by IIF, Farr and ELISA.
RESULTSDetection the Anti-dsDNA antibody of the serum sample with the methods of IIF, ELISA and Farr. The positive percentage of Anti-dsDNA in SLE is 25%, 32% and 32%, while in RA is 0, 0 and 0; in PSS is 0, 0 and 5%; in SS is 0, 0 and 0; in healthy is 0, 0 and 0.
CONCLUSIONDetection the Anti-dsDNA antibody with two method in the same time, especially with IIF and ELISA, will heighten the positive rate than with single method and will be helpful for the diagnosis of SLE.
Antibodies, Antinuclear ; analysis ; immunology ; Case-Control Studies ; DNA ; immunology ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique, Indirect ; Humans ; Immunologic Tests ; methods ; Lupus Erythematosus, Systemic ; diagnosis ; immunology ; Radioimmunoassay