OBJECTIVE:To study the effect of Erdong granules on glucose metabolism and lipid metabolism in type 2 diabetes mellitus rats.METHODS:Type 2 diabetes mellitus model was induced by giving high-fat and high-calorie diet with intraperitoneal administration of streptozotcin for eight weeks.Model rats were divided into normal group,model group,streptozotcin group and erdong granules high-dose,middle-dose and low-dose groups.The levels of FBG,LDL-C,FFA,SOD and MDA were detected and immunohistochemistry method was used to determine the morphology change of islet cell.RESULTS:The serum levels of FBS,MDA,FFA and LDL-C in Erdong granules high-dose and low-dose group were significantly decreased while the activity of SOD was increased.Erdong granules could protect islet cell.CONCLUSION:Erdong granules can notably improve glucose metabolism and lipid metabolism,antioxidant enzyme activity and inhibit oxidative stress so as to protect islet cells of type 2 diabetic mellitus rats.

OBJECTIVETo investigate the effects of open reduction by palm side for the distal radius fracture and T shape plate internal fixation with simultaneous anterior transverse carpal ligament resection for the prevention of delayed carpal tunnel syndrome after operation.

METHODSFrom March 2000 to March 2007, 32 patients (8 males and 24 females, ranging in age from 46 to 66 years) with distal radius fracture were treated with open reduction by palm side and T shape plate internal fixation with simultaneous anterior transverse carpal ligament resection; while 30 patients (7 males and 23 females,ranging in age from 45 to 65 years) only with open reduction by palm side and T shape plate internal fixation. The incidences of delayed carpal tunnel syndrome between the two groups were compared.

RESULTSAmong 32 patients treated with open reduction by palm side and T shape plate internal fixation with anterior transverse carpal ligament resection, 3 patients had delayed carpal tunnel syndrome; while in 30 patients treated with open reduction by palm side and T shape plate internal fixation, 10 patients had delayed carpal tunnel syndrome. There was significant statistically difference (P < 0.05%).

CONCLUSIONSimultaneous anterior transverse carpal ligament resection can effectively prevent the delayed carpal tunnel syndrome occurrence for the distal radius fracture with open reduction by palm side.

Aged ; Carpal Bones ; Carpal Tunnel Syndrome ; prevention & control ; Case-Control Studies ; Female ; Fracture Fixation, Internal ; Humans ; Ligaments, Articular ; surgery ; Male ; Middle Aged ; Radius Fractures ; complications ; surgery

Objective To explore the role of apoptosis of neutrophils and vascular endothelial cells and changes of relative cytokines and thrombotic factors in the pathogenesis of traumatic sepsis at the advanced stage.Methods The venous blood was collected from the patients with traumatic sepsis at advanced stage and traumatic patients without sepsis and healthy subjects.The peripheral hlood mononu- clear cells(PBMC)and neutrophils were isolated and cultured.The apoptosis of neutrophils and vascular endothelial cells was assayed,and the level of IL-4,IL-10 in PBMC culture supernatants were deter- mined,and the tissue factor(TF)and vW factor(vWF)of peripheral plasma were measured.Results The percentages of apoptosis of neutrophils and numbers of apoptotic circulating vascular endothelial cells were higher than that of traumatic patients without sepsis and healthy controls significantly.And the levels of anti-inflammatory cytokines IL-4,IL-10 and thrombotic factors TF,vWF in traumatic sepsis were elevated than that of traumatic patients without sepsis and controls too.Conclusion Immune suppres- sion and abnormal thrombotic state may be one characteristic of traumatic sepsis at advanced stage,which perhaps involveds in the pathogenesis of traumatic sepsis at the advanced stage and multiple organ dys- function syndrome.

Objective To investigate the adhesive and invasive ability of four common periodontal pathogens,Pg33277,Pi25611,Aa29522 and Fn10953 in human umbilical vein endothelial cells(HUVEC).Methods The model of infection of HUVEC by periodontal pathogens was established in vitro.The invasive ability of four periodontal pathogens in HUVEC was tested by scanning electron microscope(SEM)and antibiotic protection assays-colony-forming units(CFU).Results All of the four periodontal pathogens were found to adhere to HUVEC by SEM and invaded HUVEC at invasion numbers of(0.8±0.1)×10~8,(4.1±0.5)×10~6,(1.6±0.3)×10~6 and(5.0±0.4)×10~6 CFU/L respectively by antibiotic protection assays-CFU.The invasion efficiencies were(0.400±0.050)%,(0.021±0.003)%,(0.008±0.002)% and(0.025±0.002)%,respectively.The invasive ability of Pg33277 was significantly greater than those of the other three periodontal pathogens(P＜0.001).There was no difference in invasive abilities among Pi25611,Aa29522 and Fnl0953(P＞0.05).Conclusions All of the four common periodontal pathogens,Pg33277,Pi25611,Aa29522 and Fnl0953 could adhere to and invaded HUVEC,with Pg33277 being the strongest.

Objective To observe the effect of setting up ward assistant to improve patients'satisfaction in wards.Methods The patients' satisfaction was surveyed and the numbers of hospital patients were investigated every year from 2007 to 2009 before and after the setup of ward assistant to retrospectively analyze the effect of setting up ward assistant to improve patients' satisfaction in wards.Results After setting up the ward assistant, the degrees of satisfaction were improved (P ＜ 0.01) and the number of hospital patients and the rate of re-hospitalization were increased year by year(P ＜ 0.01).There is a positive correlation between the improvements of the satisfaction degree and the rate of re-hospitalization (r = 0.998, P ＜ 0.05).Conclusions Setting up ward assistant is helpful to improve the degrees of satisfaction and increase the amount of hospital patients.

Objective To explore the applied value of high-field intraoperative magnetic resonance imaging (iMRI) in multi-image fusion guided stereotactic brain biopsy.Methods Twenty-four patients with intracranial lesions were examined by conventional MRI and diffusion tensor imaging (DTI) with 1.5T iMRI system before operation in our hospital from June 2011 to April 2012.The digital image data and preoperative PET/CT image data were transferred into BrainLAB planning workstation and all kinds of images were automatically fused.We used the BrainLAB software to reconstruct the corticospinal tract (CST) and three-dimensional display the anatomic relationship of CST and brain lesions.Leksell surgical planning workstation was used to help to choose the best target site and the reasonable needle track.The 1.5T iMRI was used to effectively monitor the intracranial condition during brain biopsy procedure.Results Needle biopsies were successfully performed in all 24 patients and pathological diagnosis was explicitly obtained in 23 patients,leading to a diagnostic yield of 95.8％.No patient suffered from significant complications during or after surgery.Conclusions With the help of high-field iMRI,multi-image fusion guided stereotactic brain biopsy conduces to achieving the purpose of higher positive rate of biopsy and lower incidence of complications.The method with this design combining MRI-DTI fusion images with the frame-based stereotactic biopsy can play a significant role in intracranial lesions of deep functional areas.

Objective To investigate the effects of atorvastatin on parathyroid hormone1-34 (PTH1-34) induced neonatal rat cardiomyocytes hypertrophy and on the expression changes of small GTP-binding protein (K-Ras) and extracellular signal regulated protein kinases 1/2 (ERK1/2).Methods Neonatal rat cardiomyocytes hypertrophy was established with 10-7 mol/L rPTH1-34 in the presence or absence of 10-5 mol/L atorvastatin or 10-4 mol/L mevalonic acid (MVA).Cardiomyocyte diameter was measured by Motic Images Advanced 3.0 software,the synthetic rate of protein in cardiomyocytes was determined by 3 H-leucine incorporation and single-cell protein content was measured by BCA.The concentration of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were determined by ELISA.Protein expression of ERK1/2、p-ERK1/2 and K-Ras was detected by Western blot.Results Compared to PTH1-34 group,cellular diameter was decreased 12.07 μm,3 H-leucine incorporation decreased 1622 cpm/well and singlecell protein content decreased 84.34 pg,ANP or BNP concentration reduced 7.13 μg/L or 20.04 μg/L,protein expression of K-Ras,ERK1/2 or p-ERK1/2 downregulated 0.81,0.19 and 1.44 fold,respectively,in PTH1-34 plus atrovastatin co-treated cardiomyocytes (all P ＜ 0.05).Compared to PTH1-34 plus atrovastatin co-treated group,cardiomyocyte diameter increased 4.95 μm,3H-leucine incorporation increased 750 cpm/well and single-cell protein content increased 49.08 pg,ANP or BNP increased 3.12μg/L or 9.35 μg/L aud protein expression of K-Ras,ERK1/2 or p-ERK1/2 upregulated 0.52,0.06 and 1.19 fold (all P ＜ 0.05) in MVA,PTH1-34 and atrovastatin co-treated cardiomyocytes.Conclusions Atrovastatin attenuates PTH1-34 induced neonatal rat cardiomyocytes hypertrophy through downregulating K-Ras and ERK1/2 pathway.

Objective To improve the core competency and comprehensive quality of fresh nurse, and lay the foundation for future development. Methods Three stages standardized core competency training were employed for 2006-2007 year new graduate nurses, and compared to traditional training methods applied for 2004-2005 year new graduate nurses. Results The service attitude evaluation, professional knowledge, skills evaluation, and comprehensive quality evaluation scores of 2006-2007 year new graduate nurses were significantly higher than the 2004-2005 year new graduate nurses ( P ＜ 0. 05 ). Conclusions A planned and standardized core competency training program was more effective than traditional training methods, and helpful to promote stability and comprehensive quality of nursing staff.

Objective To investigate the application effect of hierarchical health education on hemodialysis patients.Methods 6 months of hierarchical health education were conducted on 100 cases of maintenance hemodialysis patients.Self-designed questionnaire was used to investigate patients' health knowledge before and after education,survival of kidney disease scale (KDQOL-SFTM) was used to study patients' quality of life before and after education,and patients' satisfaction questionnaire drawn up by nursing department was used to compare patients' satisfaction before and after education.Results Patients who had dialysis for less than 1 year,1-5 years,more than 5 years,and whose health knowledge reached the standard were respectively 19,38 and 19 after hierarchical health education,all of which were higher than before (7,17 and 11),and the differences were statistically significant (x2 =9.968,15.590,5.333,respectively; P ＜ 0.05).All items in KDQOL-SFTM got more points after hierarchical health education than before,the difference was statistically significant (P ＜0.01).Patients' satisfaction was 92％ after hierarchical health education and 78％ before,the difference was statistically significant (x2 =7.686,P ＜ 0.01).Conclusions Hierarchical health education on patients with different dialysis years is effective to enhance patients' health knowledge,harmonize nurse-patient relationship and improve patients' quality of life.

OBJECTIVETo investigate the sensitivity to bleomycin (BLM) in peripheral blood lymphocytes (PBL) among coke-oven workers.

METHODSNinty-four coke-oven workers with exposure to a high level of polycyclic aromatic hydrocarbons and 64 non-coke-oven workers (control) were recruited into this study. PBL was challenged by 8 microg/ml BLM, a known carcinogen, to induce certain amount of DNA damage, the difference of olive tail moment (TM) measured by comet assay before and after BLM treatment reflected the sensitivity towards mutagens.

RESULTSThe distribution of age, sex, and prevalence of smoking and drinking were not significantly different between these two groups. The geometric mean of urinary 1-hydroxypyrene (1-OHP) was significantly higher in coke-oven workers than in controls (9.0 versus 1.5 microg/L, t = -9.317, P < 0.01). The coke-oven workers showed significantly higher sensitivity to BLM than controls (17.7 versus 14.9, t = -2.583, P = 0.01). A large inter-group difference in sensitivity to BLM was observed in both controls and coke-oven workers. Stratification analysis revealed the significant association between high 1-OHP level (> 9.0 microg/L) and increased sensitivity to BLM (F = 4.001, P = 0.05) among coke-oven workers. Smoking subjects showed a significant higher value of sensitivity than nonsmokers in controls but not in coke-oven workers. No significant difference was observed between age, drinking status, coking history or external exposure class and BLM sensitivity.

CONCLUSIONExposure to coke oven emission could increase the sensitivity to mutagens, which might be a reason of high incidence of lung cancer among coke-oven workers.

Adult ; Benzo(a)pyrene ; toxicity ; Bleomycin ; toxicity ; Coke ; Comet Assay ; DNA Damage ; DNA Repair ; Female ; Humans ; Lymphocytes ; drug effects ; Male ; Middle Aged ; Mutagens ; toxicity ; Occupational Exposure