2.Acute toluene or xylene poisoning case analysis of domestic journals.
Jian-shu HUANG ; Yuan-ling ZHOU ; Wei-guo WAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(5):369-371
Acute Disease
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Adolescent
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Adult
;
Aged
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Female
;
Humans
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Male
;
Middle Aged
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Toluene
;
poisoning
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Xylenes
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poisoning
;
Young Adult
4.Limbs anaesthesia caused by terbutaline sulphate solution for nebulization in a child.
Shu-Mei HUANG ; Jian-Jun LIN ; Xiao-Wei HONG
Chinese Journal of Contemporary Pediatrics 2009;11(4):Inside front cover-Inside front cover
5.Construction of recombinant adenovirus vector expressing anti-VEGF hairpin ribozyme and human interleukin-24 and its cytotoxic effect on colon cancer HT-29 cells.
Shu-Jian CHANG ; Ji-Cheng YANG ; Wei-Chang CHEN
Chinese Journal of Oncology 2009;31(6):433-433
Adenoviridae
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genetics
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Cell Death
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Genetic Vectors
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HT29 Cells
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Humans
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Interleukins
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biosynthesis
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genetics
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RNA, Catalytic
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biosynthesis
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genetics
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RNA, Messenger
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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Transfection
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Vascular Endothelial Growth Factor A
;
metabolism
6.Perioperative care of total anomalous pulmonary venous connection in newborns and babies within 6 months
Yaqin SHU ; Wei PENG ; Jirong QI ; Jian SUN ; Xuming MO
Chinese Journal of Thoracic and Cardiovascular Surgery 2014;30(3):156-159
Objective To summarize perioperative care and effect of surgical treatment of total anomalous pulmonary venous connection (TAPVC) in newborns and babies within 6 months.Methods From September 2001 to May 2013,ninetytwo consecutive patients with TAPVC received surgical correction under cardiopulmonary bypass (CPB) with moderate or deep hypothermia.The anatomic subtype included supracardiac type 48,intracardiac type 36,infracardiac type 6 and mixed type 2.There were 36 newboms.Pathological diagnosis was made by echocardiogram,computerized tomography and operative findings during operation.The option of procedure was determined by findings.Respirator assisted breathing after operation and cardiac function was maintained by a variety of positive inotropic drugs,to prevent the happening of the pulmonary hypertension crisis.Results Severely low cardiac output syndrome occurred in 8 patients and they died within 24 hours after operation.The remaining cases postoperative recover smoothly and receive postoperative follow-up about 1 month to 12 years,3 cases of postoperative pulmonary vein stenosis (PPVS) occurred.Conclusion Total anomalous pulmonary venous connection should be surgical treatment as soon as possible with satisfied effect.The key of perioperative care is maintenance of left heart function,negative balance of liquid,prevention and treatment of pulmonary hypertension.
7.Quantitative assessment of left ventricular systolic synchrony in patients with chronic renal failure using real-time three-dimensional echocardiography
Shu-jiao, JI ; Jian-jun, YUAN ; Chang-hua, WEI
Chinese Journal of Medical Ultrasound (Electronic Edition) 2008;5(2):267-272
Objective To quantitatively evaluate the left ventricular systolic synchrony in patients with chronic renal failure (CRF) by real-time three-dimensional echocardiography (RT-3DE). Methods Thirty patients with CRF and twenty-five normal subjects were enrolled in this study.The M-mode ejection fraction (M-EF), left ventricular end diastolic internal diameter (LVIDd), interventricular septum diameter (IVSd), left ventricular posterior wall diameter (LVPWd) were obtained on two-dimension. The global and regional volume-time curves were obtained on three -dimensional. The end diastolic volume (LVEDV),end systolic volume (LVESV) and ejection fraction (LVEF) of left ventricule, the time to minimal systolic volume (Tmsv) of 16,12,6-segmental standard deviation (Tmsv16, 12, 6-SD), maximal difference (Tmsv16, 12, 6-Dif) were derived from Qlab software . The above parameters as a percentage of the cardiac cycle with different heart rates between patients were also calculated from the Qlab software, which were Tmsv16, 12, 6-SD% and Tmsv16, 12, 6-Dif %, rspectively. Results The indices of HR, LVIDd, IVSd, LVPWd and LVESV were significantly higher while LVEF was significantly lower in the CRF group than those of the control group (P<0.05 or P<0.01); The indices of systolic asynchrony of Tmsv16,12-SD%, Tmsv16,12-Dif, Tmsv16 ,12 ,6-SD/R-R%,Tmsv16,12,6-Dif/R-R% were significantly larger in the CRF group than those of the control group(P<0.05 or P<0.01). Conclusions RT-3DE provides a simple, intuitional and noninvasive approach to assess the systolic synchrony of all the LV segments simultaneously and LVEF in patients with chronic renal failure.
8.Study on the health standard for phosphorus pentasulfide in the workshop air.
Chun-Mi LAI ; Shu-Bo LIU ; Shun TAO ; Jian-Yun DAI ; Yun GAO ; Wei-Jun LI ; Shu-Qiao CAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(4):310-311
Adult
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Air Pollutants, Occupational
;
adverse effects
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Chemical Industry
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Female
;
Humans
;
Insecticides
;
adverse effects
;
Male
;
Maximum Allowable Concentration
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Middle Aged
;
Occupational Diseases
;
chemically induced
;
diagnosis
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Organothiophosphorus Compounds
;
adverse effects
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Phosphorus Compounds
;
adverse effects
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Sulfides
;
adverse effects
9.Chemical constituents from bark of Juglans mandshurica
Jin-Lan WANG ; Shu-Xia ZHANG ; Tie-Jun LI ; Wei-Qi ZHANG ; Jian-Jun WANG ; Shu-Jun ZHANG ;
Chinese Traditional and Herbal Drugs 1994;0(04):-
Objective To study the chemical constituents from the bark of Juglans mandshurica. Methods The compounds were isolated and purified by column chromatography on silica gel,HPLC,and recrystallization.Their structures were elucidated by the physicochemical and spectroscopic evidences. Results Fifteen compounds were identified as:4,8-dihydroxynaphthalenyl-O-?D-(6′-acetoxyl)gluco- pyranoside(Ⅰ),dihydrokaempferol(Ⅱ),juglone(Ⅲ),daucosterol(Ⅳ),kaempferol(Ⅴ),4,8-dihy- droxynaphthalenyl-1-O-?-D-[6′-O-(3″,5″-dimethoxy-4″-hydroxybenzoyl)] glucopyranoside(Ⅵ), kaempferol-3-O-?-L-rhamnoside(Ⅶ),3,3′-dimethoxylellagic acid(Ⅷ),naringenin(Ⅸ),quercetin (Ⅹ),reginolone(Ⅺ),quercetin-3-O-?-L-rhamnoside(Ⅻ),naringenin-7-O-?-D-glucoside(ⅩⅢ),4,8- dihydroxynaphthalenyl-1-O-?-glucoside(ⅩⅣ),4,5,8-trihydroxy-?-tetralone-5-O-?-D-[6′-O-(4″-hy- droxy-3″,5″-dimethoxy-benzoyl)] glucoside(ⅩⅤ).Conclusion CompoundⅠ(juglamanol)is a new compound.CompoundsⅡ,Ⅶ—Ⅸ,Ⅻ,andⅩⅢare isolated from plants of Carya Nutt.for the first time.
10.Enzymatic cyclization of peptides using immobilized sortase A.
Shu-xiang ZHANG ; Min-zhi LIU ; Yan YANG ; Ke-di CHENG ; Jian-qiang KONG ; Wei WANG
Acta Pharmaceutica Sinica 2015;50(5):627-632
Peptide cyclization, a pivotal approach to modifying linear precursors of proteins and pepticles, has been used to enhance their biological activities and serum stabilities. Recently, sortase A (SrtA) from Staphyloccus aureus becomes a promising new technology for efficiently incorporating site specific modifications into proteins, conjugating the cell surface and cyclizing the linear peptides. In this study, we constructed two recombinant expression systems, one with chitin binding domain and the other with six-histidine tag and chitin binding domain on the N-terminal of SrtA, separately. The results of enzymatic kinetics indicate that the two recombinant tags do not impair the transpeptidase activity of SrtA compared with the standard reaction reported under the same reaction condition. The two synthesized peptides with N-ternimal three glycines and C-terminal penta-amino acid motif, LPETG, were cyclized using immobilized and recycled SrtA. The SrtA-based cyclization promises to represent a simple method for easy and efficient enzymatic synthesis of large cyclic peptides.
Aminoacyltransferases
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metabolism
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Bacterial Proteins
;
metabolism
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Cyclization
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Cysteine Endopeptidases
;
metabolism
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Enzymes, Immobilized
;
metabolism
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Kinetics
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Peptides
;
metabolism
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Peptides, Cyclic
;
biosynthesis
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Staphylococcus aureus
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enzymology