1.Touch DNA of shed skin cells from the deployed airbag to address drunken driving crimes.
Zhe ZHANG ; Hong-bin SUN ; Ji-huai LUO ; Shu-guang WEI ; Sheng-bin LI
Journal of Forensic Medicine 2014;30(4):276-278
In the criminal cases of driving under the influence (DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis. The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.
Accidents, Traffic
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Air Bags
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Alcoholic Intoxication
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Crime
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DNA/analysis*
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Genotype
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Humans
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Motor Vehicles
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Skin/cytology*
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Touch
2.Observation on therapeutic effect of rolling needle therapy on insomnia.
Ling LUO ; You-ping HU ; Shu-guang YU ; Ning LI
Chinese Acupuncture & Moxibustion 2006;26(3):183-185
OBJECTIVETo assess the effect-increasing action of rolling needle therapy on insomnia.
METHODSSixty-four cases were randomly divided into a rolling needle treatment group and a control group, 32 cases in each group. The control group were treated with oral administration of clonazepam 4 mg, once each night, for 4 consecutive weeks. The rolling needle therapy group were treated with the same treatment as the control group, plus the rolling needle stimulation of the back. The therapeutic effect was assessed by effective rate of sleep improvement and Pittsburgh sleep quality index (PSQI).
RESULTSAfter treatment there was a significant difference between the two groups in the score of PSQI (P < 0.01).
CONCLUSIONThe rolling needle therapy can increase the improving action of clonazepam on insomnia, and increase life quality of the patient.
Acupuncture Points ; Acupuncture Therapy ; Humans ; Needles ; Sleep ; Sleep Initiation and Maintenance Disorders
3.Clinical application of oral and lip acupuncture.
Jing WANG ; Ling LUO ; Yong TANG ; Shu-Guang YU
Chinese Acupuncture & Moxibustion 2013;33(3):263-266
The origin, manipulations, including needling tools, postures of patients, selection of points, sterilization and specific manipulations, indications, instructions and contraindications of oral and lip acupuncture are sorted and summarized. It lays foundation to the national standard of oral acupuncture manipulations. It is also supposed to be adopted as clinical practice standard, and is going to be popularized in clinics.
Acupuncture Points
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Acupuncture Therapy
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Humans
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Lip
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physiology
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Mouth
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physiology
4.Aberrance analysis of mitochondrial DNA in a family with hereditary ataxia in Guangxi province.
Jing WANG ; Hui-hua LIU ; Shu-guang LUO
Chinese Journal of Medical Genetics 2006;23(3):323-325
OBJECTIVETo investigate the point mutations of mitochondrial DNA in the families with hereditary ataxia.
METHODSPolymerase chain reaction and single strand conformation polymorphism (SSCP) were used to analyze the mitochondrial DNA extracted from human peripheral white blood cells from the families with HA and 35 normal controls. Sequencing was performed to search the point mutations in mitochondrial DNA of those subjects whose results of SSCP were abnormal.
RESULTSA mitochondrial DNA point mutation 11893(A>G) was identified in 2 patients and 1 family member without symptoms.
CONCLUSIONA new point mutation 11893(A>G) of detected mitochondrial DNA may be relative to hereditary ataxia.
Aged ; Base Sequence ; China ; DNA Mutational Analysis ; DNA, Mitochondrial ; chemistry ; genetics ; Family Health ; Female ; Humans ; Male ; Middle Aged ; Pedigree ; Point Mutation ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Spinocerebellar Degenerations ; genetics
5.Lack of association between three single nucleotide polymorphisms in the PARK9, PARK15, and BST1 genes and Parkinson's disease in the northern Han Chinese population.
Lan-hui ZHU ; Xiao-guang LUO ; Yi-shu ZHOU ; Feng-rui LI ; Yi-chun YANG ; Yan REN ; Hao PANG
Chinese Medical Journal 2012;125(4):588-592
BACKGROUNDParkinson's disease (PD) is an autosomally inherited neurodegenerative disease in elderly people. The etiology of PD has long been thought to be associated with both genetic and environmental factors. To explore potential genetic risk factors for PD in the northern Han Chinese population, we investigated three single nucleotide polymorphisms (SNPs) (rs4538475, rs11107 and rs12564040) in the BST1, PARK15 and PARK9 genes.
METHODSGenomic DNA from 215 PD patients and 212 matched controls was amplified in two independent PCR systems and subsequently genotyped by digestion with the endonuclease PstI. Genetic parameter and association studies were carried out with SPSS 13.0 and PLINK 1.07 software.
RESULTSWe could accurately detect all genotypes in the three loci with the PCR-RFLP or mismatched PCR-RFLP techniques. The observed heterozygosities of the rs4538475 and rs11107 loci in PD and control groups ranged from 0.460 - 0.481 and 0.410 - 0.441, in BST1, PARK15 respectively, while we detected no heterozygosity at the rs12564040 locus in PARK9. The similar distributions of genotypic frequency between both groups suggest that the three SNPs investigated in this study are unlikely to play roles as common risk factors or pathogenic mutations for PD in northern Han Chinese.
CONCLUSIONThe SNPs investigated in the BST1, PARK15 and PARK9 genes associated with PD susceptibility are not associated with PD in the northern Han Chinese population.
ADP-ribosyl Cyclase ; genetics ; Adult ; Aged ; Aged, 80 and over ; Antigens, CD ; genetics ; Asian Continental Ancestry Group ; genetics ; F-Box Proteins ; genetics ; Female ; GPI-Linked Proteins ; genetics ; Genetic Predisposition to Disease ; genetics ; Humans ; Male ; Middle Aged ; Parkinson Disease ; genetics ; Parkinsonian Disorders ; genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; genetics ; Young Adult
6.Experience and discussion on the national standard Standardized Manipulation of Acupuncture and Moxibustion. Part 8: Intradermal Needle.
Ling LUO ; Cheng-Kai YUAN ; Hai-Yan YIN ; Fang ZENG ; Yong TANG ; Shu-Guang YU
Chinese Acupuncture & Moxibustion 2012;32(2):155-158
Standardized Manipulation of Acupuncture and Moxibustion Part 8: Intradermal Needle was compiled with the following principles. The compiling standard, technical features and clinic manipulations of intradermal needle were taken as the basic principle for compiling. Literature research, expert survey and clinic practice verification were applied as the drafting methods. The key issues were focused on the relationship between standardization and individualization, normalization and effectiveness, qualification and quantification. And the postural selection, reinforcing and reducing manipulations, fixing materials and embedding duration involved in intradermal needling were emphasized particularly. At the same time, details and the future way of thinking of intradermal needle were expounded in this article as well.
Acupuncture Therapy
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instrumentation
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standards
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China
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Humans
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Moxibustion
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standards
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Needles
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standards
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Reference Standards
7.Effects of tissue factor pathway inhibitor-1 on no-reflow in a rabbit model
Jing-Guang LUO ; Yun-Dai CHEN ; Feng TIAN ; Chang-Hua WANG ; Yuan L(U) ; Xiu-Xiu YANG ; Shu-Zheng L(U)
Chinese Journal of Cardiology 2009;37(12):1113-1118
Objective To investigate the role of plasma tissue factor (TF) and tissue factor pathway inhibitor-1 (TFPI-1) level and to observe the effect of extrinsic TFPI-1 on no-reflow (NR) in a rabbit model of ischemia/reperfusion. Methods Rabbits were randomized into four groups (n = 10 each): ischemic- reperfusion group (IR, subjected to 120 minutes of coronary artery occlusion and followed by 60 minutes of reperfusion); ischemic- reperfusion TFPI-1 group (100 ng/kg bolus and 1 ng · kg~(-1) · min~(-1) infusion during reperfusion) ; ischemic group (subjected to 180 minutes of coronary artery occlusion) and sham group. The NR area and ischemic area were determined by thioflavin S and Evan's blue staining in vivo. Plasma TF and TFPI-1 levels were measured before operation, before and at 120 minutes post coronary artery ligation, 10 and 60 minutes after reperfusion by ELISA. Results Plasma TF and TFPI-1 levels before and at 120 minutes post coronary artery ligation were similar among the four groups (all P > 0.05). At 10 and 60 minutes after reperfusion, the plasma TF levels in the IR group was significantly higher than those in ischemic group and sham group [10 minutes: (20.7 ±4. 1) pg/ml vs. (13.9 ±2. 2)pg/ml(P <0. 001), (20.7±4. l)pg/ml vs. (13.2±2.6) pg/ml(P<0. 001); 60 minutes; (15.8±2.6) pg/ml vs. (13.5± 1.6) pg/ml(P<0.05), (15.8 ±2.6) pg/ml vs. (12.1 ±0.7) pg/ml (P < 0. 001)] while the plasma TFPI-1 levels were similar among IR, ischemic and sham groups at 10 minutes after reperfusion and at 60 minutes after reperfusion (all P >0. 05). TFPI-1 level [(9.7 ± 1. 6) ng/ml] was significantly lower in the IR group than in the ischemic group [(11.6 ±1.6) ng/ml, P < 0. 05] and sham group [( 10. 1 ±1.3) ng/ml, P < 0. 01] . TF mRNA expression in the NR area in IR group was significantly up-regulated compared to the ischemic group (P<0. 05) and sham group (P <0. 001 ) while TFPI-1 mRNA expression was similar between IR group and ischemic group ( P > 0. 05 ) . NR severity in the ischemic-reperfusion TFPI-1 group was significantly attenuated compared to IR group (0. 39 ±0. 11 vs. 0.54±0.06, P<0.01). Conclusion Upregulated TF mRNA expression in the NR area and increased plasma TF level during reperfusion period, reduced plasma TFPI-1 level during reperfusion period as well as attenuated NR severity by extrinsic application of human rTFPI-1 in this model suggested an important role in the pathogenesis of the NR phenomenon.
8.Chemical Constituents in Volatile Oil from Fruits of Alpinia oxyphylla Miq.
Xiu-Zhen LUO ; Jing-Guang YU ; Li-Zhen XU ; Shi-Lin YANG ; Jing-Dong FENG ; Shu-Ling OU
China Journal of Chinese Materia Medica 2001;26(4):262-264
Objective:To study the chemical constituents in the volatile oil from fruits of Alpinia oxy-phylla.Method:Using GC-MS to identify the constituents.Result and Conclusion:Sixty-four compounds were identified on the basis of GC-MS, the main ones being p-cymene, valencene, linalool, myrtenal, α-pinene, β-pinene, furopelargone A and terpinen-4-ol.Three sesquiterpenes valencene, nootkanone and nootkanol have been isolated from the CHCl3 extract as check,of these 64 identiified compounds linalyl oxide, valencene, bakkenolide A, furopelargone A and 3-hydroxycalamenene are reported for the first time.
10.CA 125 expression in cervical and vaginal secretions in women in normal reproductive period.
Shu-ming HE ; Fu-qi XING ; Hong SUI ; Yong-li WANG ; Xiao-fan MAI ; Zheng-qin LUO ; Xiu-qing CHEN ; Guang-hui CHEN ; Zi-jing KONG
Journal of Southern Medical University 2010;30(1):173-175
OBJECTIVETo investigate the tumor-associated antigen CA125 expression in the serum and cervical and vaginal secretions in women during normal reproductive period, and explore the clinical value of detecting tumor markers in the cervical and vaginal secretions.
METHODSA total of 145 women in reproductive period were divided into 3 age groups (20-29 years, 30-39 years, and over 40 years), and their CA125 levels in cervical secretion, vaginal secretion and serum were detected by automatic electro-chemiluminescent immunoassay.
RESULTSCA125 levels in the cervical secretion, vaginal secretion and serum showed no significant difference between the 3 age groups (P>0.05). In each group, CA125 levels differed significantly between the cervical secretion, vaginal secretion and serum (P<0.001). In the 145 women, the average CA125 level was 497.82 - or + 75.29 U/ml in the cervical secretion, 114.66 - or + 26.40 U/ml in vaginal secretion and 18.06 - or + 3.35 U/ml in serum, showing significant differences between them (P<0.001).
CONCLUSIONCA125 expression level is significantly higher in the cervical and vaginal secretions than in the serum in women in normal reproductive period, and its levels in cervical and vaginal secretions can be more sensitive and convenient for early detection of related diseases.
Adult ; Biomarkers ; analysis ; CA-125 Antigen ; blood ; metabolism ; Cervix Mucus ; metabolism ; Female ; Humans ; Middle Aged ; Vagina ; secretion ; Young Adult