Animals ; Depression ; etiology ; metabolism ; Frontal Lobe ; metabolism ; Glutamic Acid ; metabolism ; Male ; Nitric Oxide ; metabolism ; Rats ; Rats, Sprague-Dawley ; Serotonin ; metabolism ; Stress, Physiological ; physiology ; Swimming

Objective To investigate the influence of RNA interference targeting ORC1 gene on the phenotype of rat vascular smooth muscle cells (VSMCs). Methods VSMCs were transfected with siRNA targeting ORC1 gene by liposome. The expression of ORC1 protein was detected by Western blotting. The changes of phenotype-dependent markers was analyzed by flow cytometry. Results After transfected of siRNA targeting ORC1 genes, the expression of ORC1 was lower than that in the control groups (non-transfection and negative siRNA). ORC1 gene silencing increased the expression of VSMC contractile markers ?-SM actin and SM-2, and decreased the expression of synthetic marker osteopontin. ORC1 gene silencing greatly affected VSMC differentiated morphology. Conclusion ORC1 gene silenced by RNA interference can mediate the transition of VSMCs from synthetic phenotype to contractile phenotype.

Aged ; Anthracosis ; complications ; Catheterization ; Drainage ; methods ; Humans ; Male ; Middle Aged ; Pleural Effusion ; etiology ; therapy ; Pneumothorax ; etiology ; therapy ; Retrospective Studies

OBJECTIVE: To research the content of alkaloids and polysaccharides in different varieties of Aconitum carmichaeli. METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively. RESULTS: In different varieties, the differences of total alkaloids and diester diterpenoid alkaloids were significant. However, the difference of polysaccharides wasn’t significant. The content of total alkaloids and diester diterpenoid alkaloids in processed A. carmichaeli reduced significantly while the content of polysaccharides increased slightly. CONCLUSION: The results of study could provide a scientific basis for rational breeding of A. carmichaeli.

ObjectiveTo observe the effect of rehabilitation on fractures around knee treated with external fixation.Methods48 cases with the fractures around knee who accepted early rehabilitation after external fixation had been observed for 0.5～4 years.ResultsAll the cases came up to clinical healing standards within 7 months. According to Kolmert's standard, the rate of fine and good was 89.6%.ConclusionThere was satisfactory outcome treating fractures around knee with external fixation. Early rehabilitation is important to avoid the knee joint rigor.

Adult ; Humans ; Male ; Occupational Diseases ; Pulmonary Aspergillosis

Female ; Humans ; Male ; Middle Aged ; Multiple Myeloma

Medical care on the battlefield is the core and basis of echelons of care.This review summarizes the background and characteristics of medical care units on the battlefield from the birth and growth of mobile army surgical hospitals before being replaced by forward surgical teams and combat support hospitals, since the United States Armed Forces began to lead the world military revolution during and after the World WarⅡ.Quick adaptation to the combat envi-ronment and the combat modes is the main reason that medical care units on the battlefield are adjusted continuously.This paper may provide some ideas for the development of our medical care units on the battlefield in the future.

Objective To investigate the effect of macrophage-conditioned medium on the growth of mouse myoblasts. Methods After a 3-day intraperitoneal injection with a mixture of muscle homogenate and starch,macrophages were obtained from mouse peritoneal cavity and cultured for 48 hours in serum-free DMEM/F12.Then the cultured medium was harvested.Myoblasts were obtained from newborn mice skeletal muscle and grown in DMEM/F12 supplied with 10% FBS for 36-48 hours,and then exposed to 0.5% FBS medium with or without macrophage-conditioned medium.Cells were harvested at 72 hours later,their growth was observed with Wright staining,LDH cytochemistry and immunocytochemistry. Results Macrophage-conditioned medium can maintain the normal morphology and the activity of myoblasts grown in low concentration of serum and promote their proliferation.There was a significant difference between average absorbance and integral absorbance of LDH positive cells of the two groups(P

Objective To investigate prevention and treatment of parastomal hernia. Methods Clinical data of parastomal hernia of 44 cases were analyzed respectively with special respect to clinical features and repair methods. Result Simple sutures were used in 23 cases, mesh repair in 16 cases, stoma relocation plus mesh repair in 5 cases. Postoperative recovery was satisfactory in 39 cases. Incisional infection occurred in 5 cases, and hernia recurrence developed in 3 cases (6.8%) during a follow-up of 6 to 108 months (average 49 months) in 41 patients. Conclusion The causes of parastomal hernia are miscellaneous especially incisional infection; Perioperative malnutrition must be corrected companying diseases properly controlled, and operative technique improved to prevent the incidence of parastomal hernia; Surgery is the only cure for parastomal hernia. Mesh repair and/or stoma relocation is sometimes necessary.