1.Screening of differentially expressed genes in placentas with hepatitis B virus infection by suppression subtractive hybridization technique
Gui-Qin BAI ; Ya-Fei YUE ; Shu-Lin ZHANG ; Jun CHENG ; Yan LIU ; Shu-Hong LI ; Xin-E ZHANG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(02):-
Objective To screen differentially expressed genes in placentas with hepatitis B virus (HBV)infection and to discuss the molecular mechanism of HBV intrauterine infection.Methods Thirty placenta tissue specimens from HBsAg and HBV DNA positive pregnant women were used as the study group and 30 placenta tissue specimens from normal pregnant women with HBsAg and HBV DNA negativity were served as the control group.The suppression subtractive hybridization(SSH)technique was used.Total RNAs of placenta tissue of the study group were mixed as the tester,and total RNAs of placenta tissue of the control group were mixed as the driver.A subtractive cDNA library was constructed by PCR-selective cDNA subtraction systems.Amplifications of the library were carried out with E.coil strain DH5? by reverse spot hybridization.RT-PCR confirmed that phosphatidylinositol 3-kinase(PI3K)was up-regulated in placenta tissue with HBV infection.Results Colony PCR showed that the clones contained 200-1000 bp inserts. Thirty five clones were confirmed by reverse spot hybridization and analyzed by sequencing and bioinformatics.Thirty three known genes and 2 genes with unknown function were obtained.RT-PCR preliminarily confirmed that PI3K gene was up-regulated in HBV infected placenta.Conclusions The differentially expressed genes in placentas with hepatitis B virus(HBV)infection using SSH technique has been screened out successfully.These differentially expressed genes encoding proteins participating in cell vital metabolism and malformation,and signal conduction-antiapoptosis pathway.This finding brings some new clues for studying the mechanisms of HBV intrauterine infection.
2.Influence of timing of coronal preparation on microleakage of fiber post and core systems.
Shu-e LI ; Xiang-rong CHENG ; Yu-feng ZHANG
Chinese Journal of Stomatology 2012;47(2):109-113
OBJECTIVETo evaluate the microleakage of fiber post and core systems after high-speed handpiece preparation at different time points.
METHODSThe crowns of forty-five extracted human premolar were removed and the roots were endodontically treated. The samples were devided into five groups. Root canal preparation was performed on each premolar followed by fiber post cementation and core build up. Tooth preparation was applied at 5 min in group 1, at 15 min in group 2 and at 30 min in group 3 after post cementation. Five teeth with only 5 mm apical sealing were served as a positive control group, and ten with fiber post and core build-up but no coronal preparation were taken as a negative control group. Microleakage was evaluated using a fluid filtration system. The bonding interface was observed by scanning electronic microscope (SEM).
RESULTSThe microleakage was significantly increased after coronal preparation with high-speed handpiece. The negative control group has less leakage [(1.50 × 10(-6) ± 0.37 × 10(-6)) µl×min(-1)×Pa(-1)] than the groups with coronal preparation (P < 0.05); Group 1 leaked significantly more [(6.02 × 10(-5) ± 1.02 × 10(-5)) µl×min(-1)×Pa(-1)] than group 2 [(1.50 × 10(-5) ± 0.26 × 10(-5)) µl·min(-1)×Pa(-1)] and group 3 [(1.50 × 10(-5) ± 0.39 × 10(-5)) µl×min(-1)×Pa(-1)] did (P < 0.05). Corresponding to microleakage, the micro gaps between the resin cement and dentine in group 1 were wider than those in the other groups. The coronal section was wider than the apical part.
CONCLUSIONSHigh-speed handpiece had negative effects on microleakage of fiber post and core systems. Coronal preparation should be performed 15 min or more after post cementation.
Cementation ; Dental Bonding ; Dental Leakage ; Dentin-Bonding Agents ; Humans ; Microscopy, Electron, Scanning ; Post and Core Technique ; instrumentation ; Resin Cements ; Root Canal Preparation ; Time Factors
3.Exercise training and the expression of glycogen synthase kinase 3 bate in the adipose tissues of insulin resistant rats
Li-Feng ZHANG ; Hui-Min BI ; Xing-Fu WANG ; Shu-E WANG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(10):-
Objective To investigate the effects of exercise training(ET)on the expression of glucogen synthase kinase 3 bate(GSK 3?)in the adipose tissues of insulin resistant(IR)rats on a high fat diet(HFD). Methods Thirty male Wistar rats were randomly divided into a control group(n=10)and a model group(M group,n=20).Insulin resistance was established by feeding a HFD to the M group for 4 w,while rats in the control group were fed a normal diet.The IR rats were then randomly divided into two subgroups:an IR group and an ET group.All rats in the IR and ET groups were fed a HFD,but ET was administrated to the ET group for 6w.The expres- sion of GSK 3?protein in the rats'epididymis adipose tissue was detected using Western blotting,and body weight (BW),the concentrations of serum triglyceride and cholesterol(TG and TC),fasting plasma glucose(FPG)and serum insulin(FINS),as well as insulin sensitivity index(ISI)were regularly detected.Results Compared with the con- trol group,BW and the concentrations of serum TG and TC,FPG and FINS in the model group were significantly in- creased(P<0.05),while ISI was decreased(P<0.01).Compared with the control group,there was no difference in GSK 3 protein expression in the ET group,but the expression of GSK 3?protein in the ET group was obviously de- creased in comparison with that in the IR group(P<0.05).Conclusion ET can ameliorate IR by decreasing GSK 3?protein expression in adipose tissues and enhancing the ingestion of glucose and the synthesis of glycogen.
4.Dignostic value of histogram analysis of ultrasound gray scale in differential diagnosis of triple negative breast invasive ductal carcinoma and non-triple-negative breast invasive ductal carcinoma
Maolin XU ; Fang LI ; Shu′e ZENG ; Qi TANG ; Gaolong WENG ; Qiongzhi YANG
Chinese Journal of Ultrasonography 2021;30(2):138-144
Objective:To investigate the value of histogram analysis of ultrasound gray scale in differential diagnosis of triple negative breast invasive ductal carcinoma (TN-IDC) and non-triple-negative breast invasive ductal carcinoma (NTN-IDC).Methods:Totally 195 patients with invasive ductal carcinoma confirmed by pathology in Hubei Cancer Hospital from September 2017 to July 2020 were retrospectively analyzed.According to immunohistochemical results after surgery, 195 patients were divided into TN-IDC group ( n=44) and NTN-IDC group ( n=151). All cases were retrospectively analyzed to observe the ultrasonoscopy histogram features of tumors and obtain the histogram parameters, including mean, variance, skewness, kurtosis and percentile gray-scale values. The histogram parameters of TN-IDC were compared with those of NTN-IDC. The ROC curves were constructed to observe the efficiency of differential diagnosis. Results:The values of variance, 90th and 99th percentiles in TN-IDC group were much lower than those in NTN-IDC group (all P<0.05). The values of mean, skewness, kurtosis and other percentile (1st, 10th, and 50th) values between the two groups were not significantly different(all P>0.05). The optimal cutoff value for the accurate identification of TN-IDC and NTN-IDC groups was 552.85 for variance[sensitivity 75.0%, specificity 79.5%, positive predictive value(PPV) 51.6%, negative predictive value(NPV) 91.6%, accuracy 78.5%, and area under curve 0.829, respectively]. The optimal cutoff value for the accurate identification of TN-IDC and NTN-IDC groups was 74 for 90th percentile (sensitivity 68.2%, specificity 57.6%, PPV 31.9%, NPV 86.1%, accuracy 60.0%, and area under curve 0.648, respectively). The optimal cutoff value for the accurate identification of the two groups was 107 for 99th percentile (sensitivity 75.0%, specificity 77.5%, PPV 49.3%, NPV 91.4%, accuracy 76.9%, and area under curve 0.772, respectively). Conclusions:Histogram analysis of ultrasound gray scale can provide certain value for the differential diagnosis of TN-IDC and NTN-IDC, the variance and 99th percentile values could perform better.
5.Expressions of MAP2K4 and estrogen receptor and their clinical significance in invasive breast cancer.
Journal of Southern Medical University 2016;37(4):488-493
OBJECTIVETo explore the association of mitogen-activated protein kinase kinase-4 (MAP2K4) with the pathological features, prognosis and expression of estrogen receptor (ER) in patients with breast cancer.
METHODSThe expression of MAP2K4 was detected immunohistochemically in 102 breast cancer tissues. Chi square test was used to analyze the correlation of MAP2K4 expression with the clinicopathological features of the patients. Kaplan-Meier and log rank test were used for survival analysis of the patients. Multivariate survival analysis was performed using Cox proportional hazard regression model. The correlation between the expressionsof MAP2K4 and ER was investigated using Spearman rank correlation test.
RESULTSImmunohistochemical analysis revealed low MAP2K4 expression in 55.9%(57/102) and high MAP2K4 expression in 44.1%(45/102) of the breast cancer tissues. The expression of MAP2K4 was significantly correlated with the pathological grades of breast cancer (P=0.011). Kaplan-Meier survival analysis showed that patients with a high expression of MAP2K4 had a shorter overall survival rate than those with low MAP2K4 expressions (P=0.009). Multivariate analysis identified high expression of MAP2K4 as the independent predictor of a poor outcome of patients with breast cancer. The expressions of MAP2K4 and ER were not significantly correlated, but ER-negative patients with a high MAP2K4 expressionshowed the shortest overall survival time.
CONCLUSIONOverexpression of MAP2K4 promotes the progression in breast cancer and is associated with a poor outcome of the patients. TheER-negativepatients with a high MAP2K4 expression have the shortest overall survival time, suggestingthe value of combined examination of MAP2K4 and ER in accurate estimation of the prognosis of breast cancer patients.
Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; Female ; Humans ; Kaplan-Meier Estimate ; MAP Kinase Kinase 4 ; metabolism ; Prognosis ; Proportional Hazards Models ; Receptors, Estrogen ; metabolism ; Survival Analysis
6.Dynamic monitoring of occupational hazards exposure level and evaluation the effects of prevention measures at a sentinel factory.
Jing LIU ; Yan-rang WANG ; Jin-yan SUN ; Xiao-dan XUE ; Shu-lan ZHAO ; Mei-li LI ; Jian ZHANG ; Wan-chao ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(12):919-921
7.Effects of neuroglobin gene transfer in vivo on hearing response properties of neurons in the inferior colliculus in mice after administration of sodium salicylate.
Shi-Hua YIN ; E-mail: SHUSHENGGONG@HOTMAIL.COM. ; Shu-Sheng GONG ; Kai-Sheng YAN ; Sui LI ; Pei CHEN ; Guang-Li CHEN
Acta Physiologica Sinica 2005;57(4):529-536
The effects of neuroglobin (NGB) gene transfer in vivo mediated by GeneJamer on the hearing response properties of the inferior colliculus (IC) neurons in mice after administration of sodium salicylate were studied. Forty-eight Kunming mice were divided into 4 groups (n=12 in each group): Group A1 (negative control);Group A2 (positive control);Group B, sodium salicylate (450 mg/kg every day) + pEGFP-C1;Group C, sodium salicylate (450 mg/kg every day) + pEGFP-NGB. The GeneJamer and pEGFP-NGB were mixed and injected into IC neurons in mice. The expression of NGB mRNA and protein of IC neurons in mice was detected by using RT-PCR and Western blot methods. The intensity-rate functions, intensity-latency functions and frequency-turning curves in IC neurons were recorded by extracellular electrophysiological recording techniques and the effects of pEGFP-NGB transfer following injection of sodium salicylate on them were studied. It was found that: (1) The GeneJamer-mediated pEGFP-NGB could be effectively transferred into the IC brain tissues in mice and NGB could be expressed intensively. (2) The intensity-rate functions of IC neurons were raised after administration of sodium salicylate. The non-monotonic styles of intensity-rate functions in groups A1, A2 and C were accounted for 74.6%, 72.2 %, 59.3 %, respectively, and the function in group B for 47%. There were significant differences between group B and groups A1, A2 or C (P<0.01, P<0.01, P<0.05). (3) The intensity-latency functions in IC neurons were reduced after administration of sodium salicylate. The non-monotonic styles of intensity-latency functions in groups A1, A2 and C were accounted for 3.2 %, 5.1 %and 21 %, respectively, and that in group B for 45.5 %. There were significant differences between group B and groups A1, A2 or C (P<0.01, P<0.01, P<0.05, respectively). (4) The frequency-turning curves in groups A1 and A2 were sharpened. In 72 acoustic neurons recorded in the group B, the frequency-turning curves from 53 neurons were broadened while those of the rest were sharpened. In group C the frequency-turning curves recorded from 12 of 67 acoustic neurons were broadened while those of the remaining were sharpened. These results suggest that in vivo transfer of NGB gene is highly expressed in IC neurons in mice. In vivo transfer of NGB gene reverses the change of intensity-rate functions, intensity-latency functions and the code styles after administration of sodium salicylate in IC neurons in mice.
8.Resveratrol inhibits the electrical activity of subfornical organ neurons in rat.
Ming LI ; E-mail: WQS413926@163.COM. ; Qing-Shan WANG ; Yi CHEN ; Ze-Min WANG ; Zheng LIU ; Shu-Mei GUO
Acta Physiologica Sinica 2005;57(4):523-528
The effects of resveratrol on the discharges of neurons in rat subfornical organ (SFO) slices were examined by using extracellular recording technique. The results are as follows: (1) In response to the application of resveratrol (1, 5, 10 mumol/L, n=65) into the superfusate for 2 min, the spontaneous discharge rate of 60/65 (92.3%) neurons was significantly decreased in a dose-dependent manner;(2) Application of L-glutamate (0.3 mmol/L) into the superfusate led to a marked increase in discharge rate of all 12 (100%) neurons in an epileptiform pattern. The increased discharges of 10/12 (83.3%) neurons were suppressed by application of resveratrol (5 mumol/L);(3) In 8 neurons, the selective L-type calcium channel agonist, Bay K8644 (0.1 mumol/L), induced a significant increase in discharge rate of all 8 (100%) neurons. The increased discharges of all 8 (100%) neurons were suppressed by resveratrol (5 mumol/L);(4) In 14 neurons, nitric oxide synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME) 50 mumol/L significantly increased the discharge rate of 11/14 (78.6%) neurons. Resveratrol (5 ?mol/L) applied into the superfusate reduced the increased discharges of 9/11 (81.8%) neurons;(5) In 12 neurons, the large-conductance Ca(2+)-activated K(+) channel blocker tetraethylammonium chloride (TEA) 1 mmol/L significantly increased the discharge rate of 10/12 (83.3%) neurons. Resveratrol (5 mumol/L) inhibited the increased discharges of 9/10 (90%) neurons. These results suggest that resveratrol inhibits the electrical activity of SFO neurons. This effect may be related to its properties of blockade of L-type voltage-gated calcium channel and nitric oxide (NO) promoting, and probably has no association with large-conductance Ca(2+)-activated K(+) channel.
9.Effect of extra-high-voltage power line on blood system.
Tian-tong TANG ; Jiong-li HUANG ; Xiao-wu PENG ; Jing ZHENG ; Yu-yu WANG ; Guo-cheng HU ; Shu GUO ; Qin-zhi WEI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(12):906-909
Adolescent
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Blood Cell Count
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Child
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Copper
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blood
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Electromagnetic Fields
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adverse effects
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Female
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Humans
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Iron
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blood
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Magnesium
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blood
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Male
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Students
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Trace Elements
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blood
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Zinc
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blood
10.Influencing factors for hierarchical medical system:interpretative structural modeling
Dexin MENG ; Shu′e ZHANG ; Chao FAN ; Yifu RU ; Haiyan LI ; Yu SHI ; Fengzhe XIE ; Libin YANG ; Mohan CHI ; Dachuan MAO ; Tao SUN
Chinese Journal of Hospital Administration 2016;32(7):481-484
Objective To build a multi‐level hierarchical structure model of the influencing factors for hierarchical medical system ,to identify the role relationship between all the factors and transmission pathways ,and to recommend on developing China′s hierarchical medical system . Methods Thirty influencing factors were identified in a screening based on literature review for the hierarchical medical system .On such basis ,16 influencing factors were identified by three health policy experts ,which affect operations of the current system .Interpretative structural modeling was called into play in the end to analyze the hierarchy relationship between various influencing factors and the conduction loops .Results There exist among the 16 factors a 3‐level hierarchical structureand two conduction loops .The factor directly limiting the hierarchical medical system is two‐way referral,and most internal core drivers arehuman resources development and governance mechanism.By means of self‐growth and external constraints ,they exert their influence on the operation of hierarchical medical system .Conclusions There are interactive hierarchical effects among the factors ,merging into three node elements of functional role,inter‐entity relationshipand patient participation.