Anemia, Hemolytic, Autoimmune ; therapy ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Female ; Humans ; Infant ; Rituximab

Animals ; Herpesviridae ; genetics ; metabolism ; Humans ; Viral Proteins ; chemistry ; genetics ; metabolism

Codon ; Herpes Simplex ; virology ; Herpesvirus 1, Human ; genetics ; metabolism ; Humans ; Protein Binding ; Protein Biosynthesis ; Viral Proteins ; genetics ; metabolism

Humans ; Male ; Osteosarcoma ; complications ; diagnostic imaging ; surgery ; Ultrasonography ; Wounds and Injuries ; complications ; Young Adult

Accidents, Occupational ; mortality ; Humans ; Hydrogen Sulfide ; poisoning

Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; Viral Envelope Proteins ; genetics ; metabolism

Adolescent ; Child, Preschool ; Eosinophilia ; complications ; Female ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; complications

?-arbutin is biosynthesized by whole cell method with Xanthomona maltophilia BT-112.The conditions for cell biosynthesized ?-arbutin are investigated as follows:temperature,25℃;concentration of hydroquinone,30mmol/L;mol ratio of sucrose and hydroquinone,20∶1;time course of ?-arbutin biosynthesis,45 hours;rotational speed,160r/min;concentration of Xanthomona maltophilia BT-112,85g/L;concentration of K-2HPO-4-KH-2PO-4 buffer solution,25mmol/L;pH of K-2HPO-4-KH-2PO-4 buffer solution,8.0.Under the above optimal conditions,the maximum of molar conversion yield based on the amount of hydroquinone supplied reaches 86.7%.

OBJECTIVETo compare the anti-oxidative stress effects of 5 classical traditional Chinese herbal recipes in rats with dimethylnitrosamine (DMN)-induced cirrhosis.

METHODSDMN was injected intraperitoneally three consecutive days a week to male Wistar rats for 4 weeks to develop cirrhosis model, and the model cirrhotic rats were randomly grouped and administered with 5 traditional Chinese classic recipes, i. e. Yiguanjian; Huangqi Decoction; Yinchenhao Decoction; Xiayuxue Decoction and Xiaochaihu Decoction, by gastrogavage, respectively, for 2 weeks. Changes in activity of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and malondialdehyde (MDA) content in liver tissue, and serum parameters of liver function were determined.

RESULTSGST activity and MDA content increased significantly in model rats, peaked at week 2 (liver fibrosis formation period) and week 4 (cirrhosis formation period), respectively, while activity of T-SOD and GSH-Px decreased markedly. As compared with the model control group at week 6, the degree of cirrhosis was evidently milder with lower level of MDA and GST and higher T-SOD activity in rats treated by Huangqi Decoction and Yinchenhao Decoction.

CONCLUSIONAnti-oxidative stress action should be one important mechanism for Yinchenhao Decoction and Huangqi Decoction in reversing liver cirrhosis of rats. The action of Yinchenhao Decoction focuses on eliminating hepatic lipid peroxide and that of Huangqi Decoction emphasizes for enhancing anti-oxidation ability of the organism.

Animals ; Chemical and Drug Induced Liver Injury ; drug therapy ; Disease Models, Animal ; Drug Compounding ; Drugs, Chinese Herbal ; therapeutic use ; Glutathione Peroxidase ; metabolism ; Glutathione Transferase ; metabolism ; Liver Cirrhosis ; chemically induced ; drug therapy ; metabolism ; Male ; Malondialdehyde ; metabolism ; Medicine, Chinese Traditional ; Oxidative Stress ; drug effects ; Rats ; Rats, Wistar ; Superoxide Dismutase ; metabolism

AIM: To study the effect of wild-type p53 gene on the differentiation, apoptosis and expression of scavenger receptor CD36 in U937 cells. METHODS: Recombinant adenovirus vector with wild-type p53 gene was constructed and used to transfect U937 cells. With the expression of wild-type p53 gene following adenoviral infection, transfected U937 cells were largely promoted to differentiate into macrophages. RESUITS: Trypanblue-staining test demonstrated that the percentage of positive cells increased from (14.2±5.5)% to (64.6±9.2)% and nitroblue tetrazolium (NBT) reduction test reached similar results (6.3±1.8)% vs (49.7±12.6)%. Furthermore, CD36 mRNA was up-regulated as confirmed by RT-PCR. The increased expression level of CD 36 was also detected by flow cytometry analysis. CONCLUSION: These results suggest that wild-type p53 gene can affect U937 cells differentiation and apoptosis, up-regulate expression of scavenger receptor CD36. It may have a potential significance on atherogenesis.