Adult ; Calmodulin-Binding Proteins ; metabolism ; Desmin ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Leiomyoma ; metabolism ; pathology ; surgery ; Leiomyosarcoma ; pathology ; Spinal Canal ; Spinal Neoplasms ; metabolism ; pathology ; surgery ; Young Adult

Anemia, Hemolytic ; etiology ; Child ; Female ; Humans ; Insecticides ; poisoning ; Trichlorfon ; poisoning

OBJECTIVETo observe the pharmacodynamics of Jingjielianqiao decoction in promoting the healing of acute radiation-induced skin ulcers in mice.

METHODSSkin ulcers were induced in mice by radiation, and Jingjielianqiao decoction, rivanol or normal saline was applied for the treatment. The swelling of the legs was measured in mice using volumetric method, and the ulcer area after treatment was compared among the 3 groups.

RESULTSNo significant difference was found in the anti-inflammatory effects between Jingjielianqiao decoction group and rivanol group (P>0.05), but a significant difference was noted between Jingjielianqiao decoction group and normal saline group and also between rivanol group and normal saline group (P<0.01). Significant differences were observed in the ulcer healing among the 3 groups (P<0.01).

CONCLUSIONJingjielianqiao decoction can significantly suppress radiation-induced acute inflammation and shows better effect than rivanol in promoting the healing of acute radiation-induced skin ulcer in rats.

Animals ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; therapeutic use ; Female ; Phytotherapy ; Radiodermatitis ; drug therapy ; Rats ; Rats, Wistar ; Skin Ulcer ; drug therapy ; etiology ; Wound Healing ; drug effects

Protein-protein interactions (PPI) are involved in a variety of biological processes, including cell-to-cell interactions, metabolism and development control. The misregulation, post-translational modification and interference of PPI are related to a variety of human diseases, making the regulation of these interactions a very attractive field of drug discovery. In recent years, the interaction between MDM2 and p53 has become a research hotspot, which plays an important role in the treatment of tumors. But unfortunately there are no such inhibitors approved all over the world. In this view, recent advances of MDM2-p53 inhibitors were briefly described and its inhibitors with potential therapeutic activities in clinical studies were introduced.

Objective:To explore the value of cardiovascular MR (CMR) T 1 mapping in evaluating myocardial injury in patients recovered from COVID-19. Methods:The clinical and image data of 15 patients with COVID-19 (9 with moderate clinical manifestation, 6 with severe clinical manifestation) who underwent CMR screening at 3 months after being discharged from the Second People′s Hospital of Fuyang City during May 2020 to June 2020 were prospective collected. Fifteen COVID-19 patients were selected as case group, and another 11 healthy volunteers were selected as control group. A standardized CMR protocol included cine, native and enhanced T 1 mapping, late gadolinium enhancement (LGE). Cardiac functional parameters, native T 1 value of left ventricular and extracellular volume fraction (ECV) were measured. One way ANOVA was used to assess the difference between CMR parameters among moderate and severe manifestation groups and control group, and LSD- t was used to assess the difference between the three groups. Results:LGE value was negative in all subjects. ECV values were higher in recovered COVID-19 patients with either moderate (27.9%±2.7%) or severe manifestation (30.0%±3.7%) than control group (23.2±1.9%) ( P<0.05); there was no significant difference of ECV values between recovered COVID-19 patients with moderate and severe manifestation ( P=0.100). There was no difference of native T 1 values and other functional and morphologic parameters of left ventricular and right ventricular among recovered COVID-19 patients with moderate and severe manifestation and control group ( P>0.05). Conclusion:CMR myocardial tissue ECV increase in patients who recovered from COVID-19, suggesting subclinical myocardial injury.

OBJECTIVETo explore the effect of the transporter 1 associated with antigen processing (TAP1) gene 637 A/G polymorphism on the risk of metabolic syndrome(MS).

METHODSA case-control study was conducted on 138 based-community patients (68 males and 70 females, 61.31 +/- 11.00 years old) diagnosed as MS with 162 healthy subjects (74 males and 88 females, 48.73 +/- 11.66 years old) came from the same origin as cases. The allele polymorphisms TAP1 637 A/G was examined by the specificity restriction fragment length polymorphism-polymerase chain reaction(RFLP-PCR) method with genomic DNA. The effect of TAP1 637 A/G polymorphisms on MS were analyzed by multivariable unconditional logistic regression models.

RESULTSThe TAPI 637 A/G allele genotypes frequencies (83.3%, 16.7%) contribution in control group were consistent with the distribution predicted by Hardy-Weinberg equilibrium (chi2 = 1.46, P > 0.05). TAP1 637 G allele genotypes frequencies (26.1%) of cases were significantly higher than controls (16.7%) with P = 0.005. There were significant differences of AA (58.0%), AG (31.9%) and GG (10.1%) genotypes in cases than controls, AA (68.5%). AG (29.6%) and GG (1.9%) for recessive model and addictive model after age was adjusted with P value as 0.006 and 0.044, but no significant differences for dominant model (P = 0. 298). Results from recessive model with OR = 6.62, 95% CI :1.73-25.31, Addictive model with OR = 1.56, 95% CI:1.01-2.41 and one-way ANOVA analysis showed that systolic blood pressure(SBP) and diastolic blood pressure (DBP) levels of GG genotype were significantly higher than AA or AG genotype (P < 0.05) whereas no significantly statistical differences for other clinical characteristics.

CONCLUSIONThe TAP1 637 allele A to G alteration or genotype AA to GG and AG to GG alterations could increase the risk of MS significantly, especially for SBP and DBP levels, and this positive association results might be helpful to support the biological role of TAP1 in MS but in need of larger sample size to provide more powerful evidences.

ATP-Binding Cassette Sub-Family B Member 2 ; ATP-Binding Cassette Transporters ; genetics ; Adult ; Case-Control Studies ; Female ; Genetic Predisposition to Disease ; Humans ; Male ; Metabolic Syndrome ; genetics ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length

OBJECTIVETo explore the awareness on sever acute respiratory syndrome (SARS) and public health emergencies among general publics.

METHODSA cluster sampling method was implemented in Harbin and Jiagedaqi district of Daxinanling of Heilongjiang province. Research subjects were divided into three groups as city, township and rural areas and were given questionnaires to fill in. Data was analyzed with Epi-data and SPSS.

RESULTS2003 available questionnaires were collected. The general publics well understood the knowledge on public health emergencies and the SARS with the whole recognition rate more than 60 percent. During the epidemics, people in city, town and countryside were calm (71.7%). The rates of attitude towards the government were significantly different among the subjects living with the city, town or rural areas. The city group expressed the highest favor to the government and media, 71.8% of them gave the credit on the control of SARS to the effective method taken by the government and 65.0% of them showed that they had enough confidence on the governmental ability of dealing with crises while the countryside group trusted the hospitals and relative specialists the most.

CONCLUSIONIt is essential for the government to interact and communicate with the publics through media, medical and related institutions when confronting with the public health emergencies. Publicity on health knowledge and coping system on emergency should play key roles in the development of an effective public health system while the government should lead the battle.

China ; Data Collection ; Emergencies ; Health Knowledge, Attitudes, Practice ; Humans ; Public Health ; Rural Population ; Severe Acute Respiratory Syndrome ; Urban Population

OBJECTIVETo study the effect of podophyllotoxin nanostructured lipid carriers (POD-NLC) on immortalized human cervical epithelial cells (H8) infected with HPV in vitro.

METHODSPOD-NLC was prepared by emulsion evaporation method and characterized using transmission electron microscopy, Zetasizer analyzer and high-performance liquid chromatography (HPLC). H8 cells were treated with different concentrations (0.0001-1 µg/ml) of POD-NLC, free POD, or blank nanostructured lipid carriers (NLC), and the cell proliferation was assessed using MTT assay to evaluate the cytotoxic effects. The changes of cell morphology were observed using fluorescence microscopy, and the cell cycle changes and cell apoptosis were analyzed using flow cytometry.

RESULTSPOD-NLC showed a spherical or elliptical shape with good stability in vitro. The average particle size of POD-NLC was 85.6∓10.25 nm, with a Zeta potential of 26.2∓4.1 mV and entrapment efficiency of POD of (88.56∓3.1)%. POD-NLC caused a significant inhibition of H8 cell proliferation in a concentration- and time-dependent manner. At an equivalent concentration, POD-NLC produced a stronger inhibitory effect on cell proliferation than POD. The inhibition rate of H8 cells after a 48-h exposure to POD-NLC and POD reached 95.8% and 65.6%, respectively, and at the highest concentration of 1 µg/ml, the IC(50) of POD-NLC and POD was 0.015 µg/ml and 0.13 µg/ml, respectively. Blank NLC did not obviously affect the proliferation of H8 cells. POD-NLC and POD both caused obvious increases in G(2)/M phase cell percentages and induced typical apoptotic changes of the cells, and their effects were comparable (P>0.05).

CONCLUSIONCompared with POD, POD-NLC has more potent effect in inhibiting H8 cell proliferation and inducing cell apoptosis, suggesting its potential in the treatment of cervical HPV infection.

Apoptosis ; drug effects ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Cervix Uteri ; cytology ; Drug Carriers ; pharmacology ; Epithelial Cells ; drug effects ; Female ; HIV Infections ; pathology ; Humans ; Lipids ; Nanostructures ; Particle Size ; Podophyllotoxin ; chemical synthesis ; pharmacology

Purpose To investigate the expression of signal transduction and activator 3 (Stat3) ,and phosphorylated Stat3 (p-Stat3) in human gastric cancer cell lines MGC-803 and BGC-823,and to explore the role of p-Stat3 in the invasion and migration of gastric cancer. Methods The expressed Stat3 and p-Stat3 in gastric cancer MGC-803 and BGC-823 cells were investigated by flow cytometry,and the migration and invasion abilities of cancer cells were observed using scratch test and in vitro Transwell test. Results Flow cytometry showed that the expression of Stat3 in MGC-803 and BGC-823 cells was basically unchanged before and after IL-6 stimulation (10 ng/mL) ,and the activated p-Stat3,however,was significantly higher after IL-6 stimulation. The activated p-Stat3 in BGC-823 cells was higher than that of MGC-803 cells (P < 0. 001) . The results of scratch tests showed that the scar healing area of BGC-823 cells was significantly larger than that of MGC-803 cells after 48 h (P = 0. 031) . Transwell cell experiments showed that the number of penetrating cells from BGC-823 cell line were significantly greater than those from MGC-803 cell line (P < 0. 001) . Conclusion Over activated p-Stat3 enhances the invasion and migration of MGC-803 and BGC-823 gastric cancer cells.

Objective: To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism. Methods: The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot. Results: MTT assay indicated bortezomib (2.5μM, 5μM, 10μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9. Conclusions: These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.