1.Changes of Bid Gene Expression in Neonttal Rats with Hypoxic-Ischemic Brain Dama ge
Ai-zhen, WANG ; Shu-ting, LI ; Xi-rong, GUO
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To study the changes and significance of Bid gene expression in hypoxic-ischemic brain damage (HIBD) in neonatal rats.Methods The animal models of HIBD were made. Total RNA from ipsilateral cerebral hemisph ere was extracted.RT-PCR was used to evaluate the levels of Bid gene expression at different time points after hypoxic-ischemic. Results The levels of Bid mRNA were higher in HIBD rats than those in normal rats.The ex pression of Bid mRNA in ipsilateral hemiphere following cerebral hypoxic-ischem ic began to increase at 6 h, increased to the highest level at 24 h, and then gr adually returned.Conclusion The upregulation of Bid mRNA can play a role in the induction of apoptosis fol lowing cerebral hypoxic-ischemic. J Appl Clin Pediatr,2005,20(2):121-122
2.Radiosynthesis and preliminary evaluation of 5-(11Cmethyloxy)-L-tryptophan as PET tumor imaging.
Shan-zhen HE ; Shu-xia WANG ; Kong-zhen HU ; Bao-guo YAO ; Gang-hua TANG
Acta Pharmaceutica Sinica 2015;50(5):565-568
The PET tracer 5-([11C]methyloxy)-L-tryptophan (5-(11)CMTP) was prepared by nucleophilic fluorination and alkylation reaction via a two-step procedure in order to develop specific tumor probe. The biodistribution and microPET imaging of 5-(11)CMTP were executed. The results unveiled that the overall radiochemical yield with no decay correction was (14.6 ±7.2) %, the radiochemical purity was more than 95% and high uptake and long retention time of 5-(11)CMTP in liver, kidney and blood were observed but low uptake in brain and muscle were found, furthermore, high uptake of 5-(11)CMTP in tumor tissue was observed. It seems that 5-(11)CMTP will be a potential amino acid tracer for tumors imaging with PET.
Amino Acids
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Animals
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Neoplasms
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diagnostic imaging
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Positron-Emission Tomography
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Radioactive Tracers
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Tissue Distribution
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Tryptophan
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analogs & derivatives
3.Effect of Dexamethasone on bid Gene Expression and Cerebral Apoptosis in Brain after Cerebral Hypoxia-Ischemia
Ai-zhen, WANG ; Zhen-yu, ZHANG ; Yuan-ming, ZHANG ; Xi-rong, GUO ; Shu-ting, LI
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To investigate the bid gene expression and cell death in brain after cerebral hypoxia-ischemia in neonatal rats and the effects of dexamethasone(DEX)on bid gene expression,so as to elucidate the possible mechanism of the neuroprotective effect of DEX pretreatment on rats following cerebral hypoxia-ischemia.Methods Twenty-four SD neonatal rats were divided randomly into hypoxia-ischemia brain damage(HIBD),normal,dexamethasone-pretreated and 9 g/L NaCl(NS)control group.The animal models of HIBD were made.Total RNA from ipsilateral cerebral hemisphere was extracted.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate the level of bid gene expression after hypoxia-ischemia.Cerebral apoptosis was determined by terminal-deoxynucleotidy transferase mediated d-UTP nick end labeling(TUNEL).Results The levels of bid mRNA were higher in HIBD rats than those in normal rats.The number of positive apoptosis cells significantly increased in HIBD group(P
4.Influence of Dexamethasone on Cellular Inhibitor of Apoptosis Protein 1 Gene Expression and Caspase-3 Activity in Brain after Cerebral Hypoxia-Ischemia
Ai-zhen, WANG ; Zhen-yu, ZHANG ; Yuan-ming, ZHANG ; Xi-rong, GUO ; Shu-ting, LI
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To explore the cellular inhibitor of apoptosis protein 1(cIAP1)gene expression and Caspase-3 activity in brain after cerebral hypoxia-ischemia in neonatal rats and the influence of dexamethasone(DEX)on cIAP1 gene expression and Caspase-3 activity,so as to elucidate the possible mechanism of the neuro-protective effect of DEX pretreatment on rats following cerebral hypoxia-ischemia.Methods Twenty-four SD neonatal rats were divided randomly into hypoxic-ischemic brain damage group(HIBD group),normal group(NS group),dexamethasone-pretreated group(DEX group)and 9 g/L NaCl control group(NS group).The animal models of HIBD were made.Total RNA from ipsilateral cerebral hemisphere was extracted.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate the level of cIAP1 gene expression after hypoxia-ischemia.Caspase-3 relative activity of brain tissue was determined by colorimetric assay.Results The levels of cIAP1 mRNA were lower in HIBD group than those in NS group.Caspase-3 relative activity significantly increased in HIBD group(P
5.Changes in visual field and retinal nerve fiber layer thickness after Ex - press glaucoma shunt implantation
Zhen-Zhen, WU ; Wei-Lin, WU ; Guo-Wei, WU ; Shu-Nan, XU
International Eye Science 2016;16(10):1883-1885
AIM:To observe and analyze the change of visual field and retinal nerve fiber layer thickness of primary open-angle glaucoma ( POAG ) after the Ex-press glaucoma shunt implantation.
●METHODS:A total of 14 patients (24 eyes) with POAG were underwent Ex-press glaucoma shunt implantation. Visual acuity, intraocular pressure ( lOP ) , mean defect ( MD) , pattern standard deviation ( PSD ) , retinal nerve fiber layer thickness ( RNFLT ) , and corneal endothelial cell number were collected preoperatively. Visual acuity and lOP were collected in 1wk, 1 and 3mo postoperatively respectively. Also, MD, PSD, RNFLT, and corneal endothelial cell number were collected preoperatively and 3mo postoperatively respectively. Complication, the additional treatment, success rate were analyzed.
●RESULTS:There was no significant difference (P>0. 05) in visual acuity preoperative and postoperative 1wk, 1, 3mo. Visual acuity did not significantly reduce after operation. Compared with preoperative, there was significant decreased (P<0. 05) in lOP after 1wk, 1, 3mo postoperative, respectively. lOP keep stead in postoperative 3mo. The reduction of RNFLT had significant difference (P<0. 05) between preoperative and postoperative 3mo. Compared with preoperation, MD and PSD had no significant difference ( P > 0. 05 ) after postoperative 3mo. Corneal endothelial cell number decrease had significant difference (Z=-2. 585, P=0. 01) between preoperative and postoperative 3mo. Success rate: complete success: 79. 2% ( 19 eyes ); partial success:8. 3% (2 eyes);failure:12. 5% (3 eyes).
●CONCLUSION: Ex-press glaucoma shunt implantation could efficiently decrease the lOP in POAG patients. Although it results in reduction of RNFLT in short time. The visual acuity and visual field keep stable after operation. lt is a safe and effective device for treating primary open-angle glaucoma.
6.The protective effect of shenfu injection on myocardium against ischemia reperfusion injury in rats.
Shu-yun ZHEN ; Jian-guo XU ; Zhen-zhong ZHAO
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(6):541-544
OBJECTIVETo observe the protective effect of Panax Ginseng (PG), Aconitum Carmichaeli (AC), and their combination (PG-AC) on myocardial ischemia/reperfusion injury in rats.
METHODSRat's ischemia reperfusion injury model was established by ligating left anterior descending coronary artery for 60 min followed by reperfusion for 240 min. Forty male rats were randomly divided into five groups, the sham operation group, the model group, the three treated groups. The three treated groups were treated with PG, AC and PG-AC respectively by given the drugs 10 min before ischemia reperfusion, and to the sham operation group and the model group, saline was given instead. The infarction area, pathologic changes of myocardial tissue (under light and electron microscopy), activity of creatine phosphokinase (CK) and lactate dehydrogenase (LDH) in serum, content of malondialdehyde (MDA) and superoxide dismutase (SOD) in myocardial tissue were observed to evaluate the protective effect of treatment.
RESULTSThe area of acute myocardial infarction was lesser, activity of LDH and CK were lower in the three treated groups than those in the model group. Content of SOD was significantly higher and that of MDA was markedly lower in the former three than those in the model group. Light and electron microscopic examination showed that the necrotic degeneration and pathologic changes of myocardiocytes in the treated groups were significantly milder than that of the model group. As comparing the effect between the three treated groups, PG-AC showed the best, and insignificant difference was shown between PG and AC.
CONCLUSIONBoth PG and AC, and their combination have obviously protective effects on myocardium against ischemia reperfusion injury, which of PG-AC is superior to that of PG or AC used singly.
Aconitum ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Free Radical Scavengers ; pharmacology ; Male ; Myocardial Reperfusion Injury ; pathology ; Panax ; Rats ; Rats, Sprague-Dawley
7.Analysis of 105 cases of pregnancy -induced hypertension patients with Ⅲphase of retinopathy
Xiao-Yun, QIU ; Jian-Ling, GUO ; Feng-Ci, YU ; Shu-Zhen, LIANG ; Zhen-An, LI ; Qing-Quan, HE
International Eye Science 2014;(9):1699-1701
To explore the related risk factors and outcome in pregnancy- induced hypertension patients with Ⅲ phase of retinopathy.
●METHODS: A total of 105 pregnancy - induced hypertension patients with Ⅲ phase of retinopathy in our hospital from Januany 2012 to December 2013 were enrolled. Clinical date of them were collected to analyze.
●RESULTS: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy were positively correlated with the course of the disease, blood pressure, proteinuria, and it was higher occurred in cold winter and spring, timely termination of pregnancy and appropriate hormone therapy can promote the recovery of vision, and improve outcomes of pregnancy.
●CONCLUSlON: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy associated with season and disease severity. Timely treatment can restore normal vision, improve maternal and neonatal prognosis. Routine examination of fundus examination should be used as the pregnancy induced hypertension syndrome.
8.The morbidity of pneumoconiosis in Jiaozuo city.
Zhen-rong MIAO ; Xiao-fa YANG ; Lian-xia GUO ; Shu-xia SHI ; Fang-li DU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):298-299
Adult
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China
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epidemiology
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Coal Mining
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Humans
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Middle Aged
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Morbidity
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Pneumoconiosis
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epidemiology
9.Fungal detection of American ginseng seeds from Beijing and northeast area in China.
Guo-zhen ZHANG ; Shu-feng ZHANG
China Journal of Chinese Materia Medica 2002;27(9):658-661
OBJECTIVEThe quantity and genus of fungi on 13 samples of American ginseng seed from Beijing and Northeast area in China were detected.
METHODWashing and surface sterilization were used to isolate the fungi existed on seed surface and inside of the seed, respectively.
RESULTThe genera of the fungi in embryo and shell of the seeds mainly belonged to Fusarium spp., Alternaria spp., Penicilium spp., Cephalosporium sp., Rhizopus spp., and a small quantity of Mucor, Aspergillus, Tritroderma, Doratomyces and so on. The isolated fungal frequencies on the shell and in the embryo were 36%-100% and 40%-100% respectively.
CONCLUSIONThe fungal frequency of American ginseng seed was higher. There were significant differences among different seed samples, the main fungal genera and isolation frequency in the seed embryo were identical with the results from the seed shell.
Alternaria ; isolation & purification ; China ; Fusarium ; isolation & purification ; Mitosporic Fungi ; isolation & purification ; Panax ; classification ; microbiology ; Plants, Medicinal ; microbiology ; Rhizopus ; isolation & purification ; Seeds ; microbiology