1.Advance in Cerebral Ischemic Model with Middle Cerebral Artery Occlusion in Rats(review)
Chinese Journal of Rehabilitation Theory and Practice 2006;12(11):939-940
The middle cerebral artery occlusion(MCAO) in rats remained a focus cerebral ischemic model that is non-incisioned,reliable,as well as the ischemic and refusion time can be controlled,which is widely used since it was created.However,because it is needed complex operations,associatied with many factors,it is difficult to establish.Many scholars have modified the operation,shape of the occlusion line,as well as the variety,narcotic drugs and so on.
2.Progress of the regulation effect of ginsenosides on HPA axis.
Hui LI ; Shu-Ying LIU ; Bing WANG
Acta Pharmaceutica Sinica 2014;49(5):569-575
Ginseng is a typical adaptogen which has resistance to various stresses. This effect is related to the hypothalamic-pituitary-adrenal (HPA) axis. As the main active ingredients, saponin has the similar structure to steroids. The regulation characteristics of ginseng saponin on the HPA axis are narrated from the aspects of total saponin and saponin monomers in this paper after the introduction of adaptation definition and HPA axis regulation mechanisms. Pharmacological effects of ginseng saponin and the regulation effect of HPA axis are summarized finally.
Adaptation, Physiological
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Adrenocorticotropic Hormone
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secretion
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Animals
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Corticosterone
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secretion
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Ginsenosides
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isolation & purification
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pharmacology
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Humans
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Hypothalamo-Hypophyseal System
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drug effects
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secretion
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Panax
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chemistry
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Pituitary-Adrenal System
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drug effects
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secretion
3.Study on the Physico-chemical Properties of Endothelium Corneum Amylase
Ying SUN ; Tongxiang LIU ; Shu HU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To search for the optimal processing procedure and physico-chemical properties of Endothelium corneum amylase. Methods After dried under 50 ℃ ,amylase was extracted from Endothelium corneum with phosphate-buffered saline. The amylase activity was examined at different temperature,pH and different metal ions,and amylase isozyme was discerned on PAGE. Results The optimal pH,optimal temperature,and optimal substrate concentration for amylase activity were 8.67,50 ℃ and 1.8 g/mL,respectively. Under the experimental conditions,the amylase Km was 0.92 (starch was used as substrate of amylase). Four metal ions tested,including Ca2+,Fe2+,Zn2+,Mn2+,could inhibit the amylase activity to some extent. Furthermore,it showed one amylase isozyme band using PAGE,suggesting only one amylase isozyme existing in the Endothelium corneum tentatively. Conclusion The effects of metal ions should be considered in the process of Endothelium corneum amylase.
5.Expression and significance of NF-κB p65 in ethanol induced acute liver cell damage in mice.
Shu-qing JIANG ; Ning HE ; Ying-hua LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(11):840-841
Animals
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Cells, Cultured
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Ethanol
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toxicity
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Hepatocytes
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drug effects
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metabolism
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pathology
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Male
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Mice
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Mice, Inbred Strains
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Transcription Factor RelA
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metabolism
6.Curative effect of 3-dimensional conformal radiotherapy for recurrence cervix cancer:report of 42 cases
Ying TANG ; Qi ZHOU ; Xiaolei SHU ; Li LIU
Journal of Third Military Medical University 2003;0(17):-
0.05).The effective rate of group A was 52.38%(22/42),and group B was 32.35%(11/34).There was significant differences between the 2 groups(P
7.Research progress of oxytocin receptor ligands and its role in central nerve system and peripheral organs
Yajing MENG ; Yang YANG ; Ying LIU ; Shu AN ; Tianrui XU
Chinese Pharmacological Bulletin 2014;(6):752-756
Oxytocin ( OT ) is a cyclic neuropeptide containing nine amino acids residues, in addition to the traditional roles of uterine contraction and lactation, it also plays important roles in the central nervous system and other peripheral organs, such as improving schizophrenia, autism - related psychiatric and psy-chological symptoms. Oxytocin exhibits its physiological func-tions by binding to its receptor (oxytocin receptor,OTR). Cur-
rently researchers are manipulating OT system by developing new OTR ligand ( agonists and antagonists ) , hoping to prevent and treat OTR related diseases. This paper reviews the latest devel-opment of OTR agonists, antagonists and its physiological roles in central nerve system and peripheral organs.
8.Effects of ganglioside-1 loaded magnetic pluronic nanoparticles on repair of acute complete spinal cord injury
Ying LI ; Shu CHEN ; Qihuang ZHAO ; Chen GUO ; Huizhou LIU
Chinese Journal of Tissue Engineering Research 2009;13(16):3061-3064
BACKGROUND: Temperature-responsive magnetic pluronic nanoparticles possess the capacity of drug release controlled by body temperature and can penetrate blood brain barrier.OBJECTIVE: To detect the capacity of magnetic pluronic nanoparticles to carrying ganglioside-1(GM-1) and its capacity of drug release in vivo, as well as its effects on repair of spinal cord injury.DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the key laboratories of Beijing Institute for Neuroscience, Capital Medical University and Institute of Process Engineering, Chinese Academy of Sciences between June 2006 and February 2007.MATERIALS: GM-1 loaded magnetic pluronic nanoparticles were prepared by Laboratory of Separation Science and Engineering State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences. GM-1 was provided by Trb Pharma S.A of Argentina.METHODS: Twenty Sprague-Dawiey rats were developed into acute complete spinal cord injury models and were then evenly MAIN OUTCOME MEASURES: Behavior evaluation and quantitative analysis (nerve fibers and glial cells) at 4 weeks post-surgery.were significantly greater in the treatment group than in the remaining 3 groups (P < 0.05), the control groups exhibited greater numbers of nerve fibers than the sham-operation groups, and the control group 2 had higher levels compared with the control compared with the remaining 3 groups (P < 0.05). In the rostral areas, the control groups exhibited higher area ratio of glial cells than the sham-operation group (P < 0.05), and in the caudal areas, control group 2 displayed higher levels compared with the sham-operation group (P < 0.05).CONCLUSION: Magnetic pluronic nanoparticles possess drug-carrying and drug-releasing capacities. GM-1 loaded magnetic pluronic nanoparticies can repair spinal cord injury and promote nerve regeneration.
9.Preliminary study of intensity-modulated radiation therapy for recurrent cervical cancer
Qi ZHOU ; Ying TANG ; Xiao-Lei SHU ; Li LIU ;
Chinese Journal of Obstetrics and Gynecology 2000;0(11):-
0.05).The effective rate of group A was 71%(15/21)and of group B 39%(7/18),with a significant difference in the two groups (P
10.Imaging characteristics of intraparenchymal schwannoma and the related pathology
Shu-Yong LIU ; Dao-Ying GENG ; Hui-Jin HE ;
Chinese Journal of Radiology 2001;0(08):-
Objective To Analyze the imaging characteristics of intraparenchymal schwannoma and the related pathology,in order to improve the accuracy of diagnosis and be in favor of the clinics and the prognosis.Methods Four cases were confirmed to be intraparenchymal schwannoma by pathological and immunohistochemistry examination.One case was examined with precontrast and enhanced CT scanning,one with unenhanced MRI scanning,two with unenhanced and enhanced CT and MRI scanning.Their images were retrospectively analyzed.Results Of the four cases,three patients were less than 30 years old,with tumors located supratentorially.Cysts were found in all cases,with nodules on the wall in 3 cases.The nodules were enhanced markedly in two cases and moderately in one ease.In addition,calcification was detected in one case and prominent peritumoral edema existed in 1 case.The picture of the pathology demonstrated Antoni type A and Antoni type B.Immunostaining showed intense immunoreactivity for S-100 protein and Vim and negative immunoreactivity for GFAP and EMA.Conclusions Intraparenchymal schwannoma mostly occurred in juvenile,which located supratentorially in most cases.The presence of a cyst and peritumoral edema together with the tumor appears to be characteristic of intraparenchymal schwannoma.Calcification or the enhanced nodule is the helpful sign for the diagnosis.Combining the imaging findings with the pathology and immunohistochemistry results can gain the accurate diagnosis.