Objective To evaluate the expression of interleukin-6 (IL-6) on peripheral blood and adenoid in children with chronic rhinosinusitis (CRS) and adenoidal hypertrophy ,to investigate the mechanism of IL-6 on the occurrence and development of CRS .Methods A total of 216 cases of hypertrophic adenoids were collected ,including 95 CRS children with hypertrophic adenoid (CRS group) ,70 children with allergic rhinitis and hypertrophic adenoid (AR group)and 51 children with hypertrophic adenoid (control group) .The plasma IL-6 concentrations were detected by ELISA ,and the expression and distribution of IL-6 in adenoid tissue was detected by immunohistochemistry .Results The plasma IL-6 level in CRS group was (49 .8 ± 8 .3)pg/mL ,significantly higher than that of in control group (33 .6 ± 6 .9)pg/mL and in AR group (35 .9 ± 5 .6)pg/mL ,differences had statistic significance ( P< 0 .05) .However ,there was no statistically significant difference between AR group and control group .Meanwhile ,the presence of IL-6 in adenoid tissue was mainly seen in inflammation cells and fibroblasts while less in mesenchymal cells ,there were statistical differences between CRS group and control group/AR group (P< 0 .05) ,but no statistical difference between AP group and control group(P> 0 .05) .Conclusion Among children with CRS ,expression of IL-6 whether in peri-plasma or in focal tissue was higher than those without CRS ,demonstrating that IL-6 may play an important role in the pathogenesis and developemnt of CRS in chil-dren .

AIM:To demonstrate the relationship between hormones in follicular fluid and the expression of LH receptor in granulosa cells(GC) in anovulatory women with polycystic ovary syndrome(PCOS).METHODS: Follicles were obtained from 12 women with PCOS and 15 women with normal menstrual period through surgery at time between day 7 and day 10 of menstrual cycle.The accumulations of estrogen(E2),progesterone(P),luteinizing hormone(LH),follicle stimulating hormone(FSH) and insulin in follicular fluid were determined by a automatism chemiluminescent microparticle immunoassay(CMIA) for the quantitative determination.The accumulation of androstenediol(A) was determined by ELISA.The amounts of the mRNA expressions of LH receptors from GC and theca cells(TC) respectively were measured by RT-PCR using ?-actin as intra-control simultaneously.RESULTS: The levels of LH [(3.8?2.1 vs 1.7?0.8)IU/L,P

Anastomosis, Surgical ; Colon, Sigmoid/surgery* ; Colorectal Neoplasms/surgery* ; Humans ; Robotics

Objective To provide evidence for the off-indication use of voriconazole.Methods PubMed,EmBase,the CochraneLibrary and three Chinese databases (CNKI,SinoMed and WangFang) were searched through January 2016.The indications,pathogens,route of administration,cure rate and type of study were inclu-ded in the statistical analysis.All thestudies about the off-indication use of voriconazole were included,according to the established criteria.Results Thirty-nine case reports and case series involving the off-indication use and research advance of voriconazole on ocular infections,skin and soft tissue infections,bone and joint infections,abdominal infections,cardiac infection,and histoplasmosiswere included in the review.Conclusion We provided support for the treatment of fungal infections in rare sites with a low incidence rate and no clear antifungal treatment regimen.

Objective To define the questions and outcomes included in the Practice Guidelines for Individualized Medication of voriconazole and their importance ranking.Methods Using the Delphi method , experts were consulted in three rounds.Enthusiasm index , conformity indexes and authority indexes were calculated for quality control.Results Eighteen questions and 10 outcomes were defined.Recommendations of seven questions must be developed in the guideline.Four outcomes were ranked as “critical outcome”.Enthusiasm index was 90.5%.Authority indexes were all >0.75.Conformity indexes of only 2 questions and 2 outcomes were ≤15%.Conclusion Through Delphi method , questions and outcomes included in the Practice Guidelines for Individualized Medication of voriconazole were defined , which gives directions to guide-line development.

Objective To investigate selcnium(Se) levels of environment and human body in Gaomi City and Zichuan District of Shandong. Methods Lijiaying Township in Gaomi City of Weifang City, Zhaili Township and Longquan Township in Zichuan District of Zibo City were selected. Two farming soil samples at different spot, local wheat and corn, residents nail samples from 3 to 4 families were collected in each natural village in the investigated towns. The contents of Se were detected by 2,3-diamino naphthalene fluorescence method. Results Se level of the soil, wheat, corn, and nails in Lijiaying [(0.054 ± 0.019), (0.022 ± 0.009), (0.018 ± 0.007), (0.365 ± 0.108)mg/kg] was significantly lower than that in Zhaili [(0.425 ± 0.080), (0.130 ± 0.043), (0.098 ± 0.026), (0.751 ± 0.134)mg/kg] and Longquan[(0.487 ± 0.153), (0.112 ± 0.030), (0.097 ± 0.029), (0.735 ± 0.145)mg/kg;P < 0.01]. In Lijiaying, Se was deficient in soil, wheat, corn(< 0.200, < 0.025 mg/kg), above Se deficiency diagnosis and below Se-adequate level in the nail, while in Zhaili and Longquan, the Se level in the soil (0.425, 0.487 mg/kg), wheat(0.130, 0.112 mg/kg), corn (0.098, 0.097 mg/kg), nails (0.751, 0.735 mg/kg) was adequate (≥0.400 mg/kg). Conclusions The external environment is Se-deficient in Lijiaying, Se-adequate in Longquan and Zhaili. The selenium level in human body is consistent with the external environment.

Objective To observe the clinical efficacy and safety of dantrolone sodium capsules in thethrombolytic therapy of acute stroke.Methods One hundred and thirty-six patients with acute stroke were randomly divided into control and treatment groups with 68 cases per group. Control group was given routine thrombolytic therapy + low molecular weight heparin calcium 0. 4 m L per, q12 h, subcutaneous injection for 10 days + aspirin 100 mg per time, qd, orally for 3 months.Treatment group was given dantraline sodium capsules 25 mg per time, qd, orally for 3 months after thrombolytic therapy, on the basis of control group. The clinical efficacy, American institutes of health stroke scale (NIHSS) scores and adverse drug reactions were compared between two groups. Results After treatment, the total effective rates of treatment and control groups were 77. 94％ (53 cases/68 cases) and 54. 41％ (37 cases/68 cases) with significant difference (P < 0. 05) . The NIHSS scores of treatment and control groups were (16. 07 ± 1. 18) and (18. 27 ± 1. 21) at 7 days after thrombolytic therapy, and those were (12. 15 ± 1. 06) and (16. 84 ± 1. 10) at 3 months after thrombolytictherapy. The differences were statistically significant between two groups (all P < 0. 05) . The adverse drug reactions of control group were gastrointestinal reaction, hematuria, sleepiness and asymptomatic cerebral hemorrhage, which in treatment group were gastrointestinal reaction, lethargy, fatigue, dizziness, asymptomatic cerebral hemorrhage and hematuria. The total incidences of adverse drug reactions in treatment and control groups were 17. 65％ and 13. 24％without significant difference (P> 0. 05) . Conclusion Dantraline sodium capsule is effective in thrombolytic therapy for acute stroke, without increasing the incidence of adverse drug reactions.

Adenocarcinoma ; blood supply ; physiopathology ; Capillaries ; metabolism ; ultrastructure ; Cell Separation ; Cells, Cultured ; Endothelial Cells ; cytology ; metabolism ; ultrastructure ; Humans ; Microcirculation ; Stomach Neoplasms ; blood supply ; physiopathology

UNLABELLEDProminent eosinophil airway inflammation is important in the pathogenesis of asthma. There is increasing evidence that the disorder of eosinophil apoptosis contributes to the mechanism. But most of the studies have been done in vitro or on animal models, very few were done among the adult asthmatics in vivo.

OBJECTIVEThe aim of this study was to elucidate the relationship between the apoptotic eosinophils and Bcl-2 in asthmatic children in vivo.

METHODSEleven mild to moderate asthmatic patients were recruited and the range of age was 7 - 14 years (9 males, 2 females), meanwhile 7 patients with lower respiratory infection were recruited as control and the range of age was 9 - 14 years (5 males, 2 females). Before and after inhaled glucocorticoid (GC) induced sputum, bronchoalveolar lavage (BAL), bronchial mucosa specimens and peripheral blood were obtained for measuring and comparing the changes of apoptotic EG(2)(+) cell by combining the techniques of TUNEL and immunohistochemistry, meanwhile the expression of Bcl-2 in bronchial mucosa specimens was measured by using the immunohistochemical assay.

RESULTSBefore the inhalation of GC, the apoptotic EG(2)(+) cells in asthmatics were significantly lower than that in control group (P < 0.01), and the numbers of EG(2)(+) cell in asthmatics group were significantly higher than that in control group (P < 0.001). After the treatment apoptotic EG(2)(+) cells in asthmatics were increased (P < 0.01), and the numbers of EG(2)(+)cell were decreased (P < 0.01, P < 0.05 and P < 0.05, respectively), FEV(1)% was increased (P < 0.05). Before the inhalation of GC, the numbers of Bcl-2(+) cell in asthmatic airway submucosa were higher than that in control group (P < 0.05) but after the treatment the number of Bcl-2(+) cell did not change significantly. (4) Before and after GC treatment the percentages of apoptotic eosinophils of peripheral blood in vivo had no significant changes compared with those of control subjects (P > 0.05). There was a positive correlation between apoptosis of EG(2)(+) cell in sputum, BAL, airway submucosa and FEV(1)% (P < 0.05).

CONCLUSIONApoptosis of EG(2)(+) cell decreased in the airway of asthmatic children and inducing EOS apoptosis is one of the important mechanism of inhaled GC therapy for asthma.

Adolescent ; Apoptosis ; Asthma ; blood ; drug therapy ; pathology ; Bronchoalveolar Lavage Fluid ; cytology ; Child ; Eosinophils ; cytology ; Female ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Male ; Proto-Oncogene Proteins c-bcl-2 ; analysis ; Respiratory Mucosa ; chemistry ; cytology

OBJECTIVESTo investigate the effect of gamma-aminobutyric acid (GABA) on the sperm acrosin activity in normal men and positive antisperm antibody (AsAb) men.

METHODSSperm acrosin activity was detected by BAEE/ADH method.

RESULTSGABA could increase the sperm acrosin activity in normal and AsAb positive patients (P < 0.01). The results also indicated that GABA significantly increased Na(+)-K(+)-ATPase activity (P < 0.01), Ca(2+)-ATPase activity (P < 0.05) and SOD activity (P < 0.01) of sperm.

CONCLUSIONSThe results demonstrated that GABA could influence the sperm acrosin activity.

Acrosin ; metabolism ; Humans ; Male ; Spermatozoa ; drug effects ; immunology ; metabolism ; gamma-Aminobutyric Acid ; pharmacology