Ethmoid Bone ; injuries ; Facial Bones ; injuries ; Humans ; Nasal Bone ; injuries ; Orbital Fractures ; surgery ; Osteogenesis, Distraction ; Skull Fractures ; surgery

Background Cardiomyocytes apoptosis was related to oxidative stress which has been shown to play an important role in ventricular remodeling after myocardial infarction(MI).Probucol is a hypolipidemic and antioxidant agents.Several reports demonstrated its cardioprotective effect after MI.However,the exact effect of probucol on the modification of the apoptosis related gene Bcl-2 and Bax is not clear.Objective To investigate the effects of probucol on mRNA and protein expression of Bcl-2,Bax and oxidative stress in myocardial infarcted rats.Methods Forty-one SD rats that survived 24 h after ligating left anterior descending coronary artery were randomly to receive placebo-saline(5 mL/d,n=20)or probucol(probucol 60 mg/kg?d,n=21).Twelve rats underwent sham operation were served as control(n=12).Six weeks after treatment,hemodynamic pararmeters and left ventricular function were measured with catheterization.Cardiomyocytes apoptosis were determined by TUNEL method.Myocardium mRNA of Bcl-2 and Bax expression levels in the non-infarcted myocardium were as- sessed by RT-PCR.The myocardium protein expression of Bcl-2 and Bax in the non-infarcted myocardium were de- termined by Western blot.Colorimetry was used to determine oxidative metabolism index in myocardium.Results 1)Compared with the sham rats,all MI rats showed marked decrease in Bcl-2 mRNA and protein expression in myo- cardium with increase of Bax mRNA and protein expression and apoptosis index(P

0.05) between the radiotherapy and control groups,and the coverage of vascular endothelial cell was incomplete in each group;8 weeks after surgery,the intimal thickness of radiotherapy group was statistically thinner than that of control group(P

Objective To explore the effect of high dose external beam radiation on the ePTFE prosthesis-arterial anastomosis.Methods The infrarenal abdominal aorta was replaced by ePTFE prosthesis graft in 20 dogs,and all the animals were randomly divided into 2 groups,including of irradiated groups and the control groups,which were or were not associated post-operative external radiation(35 Gy) to the anastomosis.All the animals were sacrificed at 4 weeks and 8 weeks after operation for histological and immunohistochemical examination of the prosthesis-arterial anastomosis.Results There was marked histological changes caused by 35 Gy external irradiation at the prosthesis-arterial anastomosis,but no disunion,rupture,or aneurysm was found at the anastomosis.Radiation did not increase the rate of thrombosis at the prosthesis.The result of immunohistochemical examination showed that two side of the anstomosis were CD34 positive.Conclusions High dose of external beam(35 Gy) can cause marked histological changes at the prosthesis-arterial anastomosis,however,it will not exert negative effect on anastomosis in the short term.

Objective To investigate neural correlates of the generation,execution and inhibition of motor intention. Methods Behavioral performance and event-related potentials (ERP) of 14 participants were recorded when they performed a two-staged Go-Nogo task. At the stage of intention preparation,three kinds of the "what" cues were presented separately to the participants : (1) to press a response key by the left or the right hand (the instructed) ,(2) to decide freely which hand will be used (the free) ,(3) to wait for the second cue (the control). At the stage of intention execution,the "whether" cue suggested the participants execute (Go) or withdraw (Nogo) the previous intention either voluntarily or forcedly. Results The interaction effect between the "what" and the "whether" was significant(F(2,26) = 8.262, P=0.002). The forced responses were faster than those of voluntary action under both the instructed condition ((468.52 ±105.87)ms vs (546.67 ±146.33)ms) and the control condition ((536.78 ± 83.28) ms vs (589.75 ± 140.80) ms). No significant difference was found for the free condition((538.71 ± 127. 39) ms vs (561. 44 ± 146. 51) ms). At the stage of intention preparation,significant ERP effects between the instructed intention and the freely intention were revealed in bilateral frontal regions (150～200 ms) , frontoparietal area (300～700 ms)and right temporal area. At the stage of intention execution,ERP effects between voluntary inhibition and voluntary action were observed in left prefrontal area (160～220 ms) ,fro-nto-cento-parietal area and right frontoparietal area. (300～550 ms). Conclusion The motor intention may be generated in the prefrontal area and maintained in the frontoparietal network. The left prefrontal and fronto-centro-parietal areas inhibit intention in the early stage and the right frontoparietal area involves response inhibition lately.

Objective To investigate neural correlates of the retrieval of temporal order.Methods ERP of 19 channels was recorded from 17 healthy young students while they performed a recency judgment task,a recognition task and a visual discrimination task separately,which were based on Sternberg paradigm.One-way ANOVA of repeated-measurement and post-hoc paired comparisons were carried out for both the task factor(temporal order,recognition and control)and the temporal distance factor correspondingly.The latter contains 3 intervals (1,2 and 3)between the targets.Results The temporal distance effect was significant:the closer targets correspond to longer reaction time((745.5±106.1)ms,(711.7±102.2)ms,(653.7±81.5)ms;F_((1.28,20.48))=43.37,P=0.00]and higher error rate((62.8±7.0)%,(72.5±5.4)%,(84.5±6.4)%;F_((1.32,21.11))=135.99,P=0.00].Significant behavioral effect of the task factor was also found for both reaction time(F_((1.32,21.11))=135.99,P=0.00)and error rate(F_((2,32))=200.31,P=0.00).No significant behavioral difference was found between the temporal order group and the recognition group.The spatiotemporal pattern of statistical parametric mapping(SPM)suggested the ERP effects of temporal order were presented in left temporal region (260～270 ms),bilateral parietal-occipital areas(280～290 ms),and bilateral parietal lobes(300～400 ms)prominently.No ERP effect was found between the temporal order task and the recognition task.Conclusions The neural correlates of the temporal distance effect exist in bilateral parietal regions.Temporal order as well as object information processing involves similar cerebral regions during the retrieval period.

Objective To investigate the neural processing of stimulus salience and behavioral relevance with spatiotemporal patterns of event-related potentials (ERP).Methods The ERP was recorded from 20 subjects while they performed Eriksen task.Each stimulus picture contained 3 symbols:a central target (grey symbol ＜/ ＞ or X) represented pressing the corresponding button or withdrawing response respectively.And the flanking distractors were classified by both behavioral relevance ( ＜/ ＞-facilitory,X-inhibitory,H-irrelevant) and color salience (red,grey).Results Significant interaction effect was found for the reaction time(F(1.85,3521)=13.015,P＜0.001).Significant difference was found between the salient ((411.91 ±40.39) ms) and the non-salient ((420.42±38.58) ms) under the facilitory condition(t (19) =-4.858,P＜0.001).The significant ERP effects were revealed by statistical parametric mapping of F-value.The saliency effects appeared in the right occipital-temporal regions (100-125 ms),the bilateral dorsal attentional network (DAN) (150-300 ms) and the right-lateralized ventral attentional network (VAN) (300-450 ms).The effects of the behavioral relevance occurred in the occipital regions (150-200 ms),the VAN (200-300 ms) and the DAN (300-600 ms).The interaction effects of behavioral relevance between the target and the distractor occurred in the DAN (200-350 ms).Conclusion The information of the distractor salience and behavioral relevance access VAN and DAN in different stages respectively.The ERP effect in the DAN may be the neural correlation of contingent attentional capture.

Objective To investigate the protective effect of angiotensin-converting enzyme inhibitor (ACEI) on kidney function in patients with sepsis.Methods Eighty-seven patients with sepsis were randomly (random number) divided into the routine treatment (A group,n ＝45) and the ACEI intervention group (B group,n =42).Patients were managed by international guidelines for sepsis in A group and were treated with benazepril (ACEI) 20 mg a day in addition in B group.Serum creatinine and cystatin C were detected and APACHE Ⅱ scores and urine output were recorded on the 1st,3rd and 7th day.Those laboratory findings and APACH Ⅱ score were compared between two groups.The incidence of acute kidney injury (AKI) and abnormal cystatin C levels were compared between two groups.Results In B group,serum creatinine and cystatin C of patients were lower compared with A group.The incidence of AKI and abnormal cystatin C and APACHE Ⅱ scores were reduced in B group compared with the A group.Conclusions Benazepril improved sepsis-induced AKI and patient conditions.

Objective: To investigate the clinical efficacies of fire acupuncture in treat various types of acne. Methods: An open test was designed. One thousand and eighty-six patients who visited for clinical service were collected as a sample. Results: The cure and marked effective rate was 78.5% for lung heat type, 80.8% for heat toxin type, 77.4% for disharmony of the Thoroughfare and Conception Vessels type and 80.1% for blood stasis and phlegm accumulation type. Statistical analysis showed no significant differences in therapeutic effect between different types (P>0.05). Conclusion: Fire acupuncture has an exact therapeutic effect on various types of acne without toxic side effects.

Objective: To evaluate the effects of BMP 2 on wound healing of facial nerve. Methods: Facial nerve was crushed in 6 rabbits.1 ng of BMP 2 in 20 ?l of saline was injected around the injured nerve on one side, 20 ?l of saline was injected on the other side as the control.Each two animals were sacrificed 1d,2 and 4 weeks after injury.The nerve samples were studied by histological observation,electron microscopic observation and image pattern analysis.Results:Better healing was observed in BMP 2 treated nerves in 2 and 4 weeks.4 weeks after injury,the axon number on each 100 ?m2 in BMP 2 treated nerves and control ones was 0.946?0.572 and 0.614?0.430(P