The tissue engineering technology that developed in the 1980s brings a hope for the regeneration of human organs. It is feasible to construct by-pass vessels, which have no immune resistance but are capable of anticoagulation. At present, a huge number of researches have provided the necessary basis for the construction. However, there are still some difficulties on the points of induction and proliferation of endothelial cells, development of new materials for scaffold and co-culture of seed cells in scaffold. These difficulties have become the bottle neck of tissue engineered blood vessels. The tissue engineering vessels are nowadays absent in clinical practice. With the progress in researches of tissue engineering, it is strongly believed that human organs and tissues can be constructed using tissue engineering technology, thus satisfying the donors of organ transplantation.

Acupuncture Points ; Administration, Cutaneous ; Adult ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; Facial Paralysis ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

Objective To compare the efficacy and safety of intravenous infusion of recombinant human brain natriuretic peptide(rhBNP)versus Sodium Nitroprusside on treating patients with decompensated acute heart failure.Discuss rhBNP 's effect on connective tissue of heart.Methods A total of 44 patients characterized of decompensated acute heart failure were enrolled in this study.The patients were randomLy allocated to rhBNP group who receive rhBNP(1.5?g/kg bolus intravenous injection followed by 0.0075?g/(kg?min)for 24 hours,n=23)and Sodium Nitroprusside group(10 ?g/ rain for 24 hours,n=21 ).Blood pressure,heart rate,dyspnea grade,24 hours fluid in-and-output and improvement in patient symptoms and signs were evaluated and adverse events were documented.Results Dyspnea and symptom im- provements were much more significant in rhBNP group compared to Sodium Nitroprusside group(P

Cerebrospinal Fluid Rhinorrhea ; surgery ; Female ; Humans ; Middle Aged ; Semicircular Canals ; surgery

Bronchogenic Cyst ; diagnosis ; Diagnostic Errors ; Goiter ; Goiter, Nodular ; diagnosis ; Humans ; Neck

Carcinoma, Adenoid Cystic ; diagnosis ; surgery ; Endoscopy ; Humans ; Neoplasm Recurrence, Local ; diagnosis ; Nose ; surgery ; Nose Neoplasms ; diagnosis

Esthesioneuroblastoma is one of the most uncommon nasal malignancies which is characterized by low incidence and high misdiagnosis rate.The only prognosis factor is the metastasis on lymph node of neck.Its clinical symptoms are associated with tumor infiltrating extent.Pathological diagnosis is the “gold standard” on esthesioneuroblastoma,and the Kadish staging based on the image is the most important staging standard.The traditional operation therapy mode has been replaced by the combined method of operation and radiotherapy,with the adjunctive therapy of chemotherapy.

Diffusion tensor imaging is a non-invasive MRI technique which can identify changes of cerebral microstructure that CT and MRI is difficult to find, especially in the change of nerve fibers direction, which can be used for the researches of evaluation, recovery mechanism and prognosis of neurology. It has been applied in rehabilitation of motor, language and recognition of post-stroke patients.

Chromatography, Gas ; methods ; Humans ; Trifluralin ; blood

Objective:To study the isolation, culture and identification of the TMJ cells and to observe the biological characteristics of cultured fibrochondrocytes. Methods:The TMJ discs were dissected from two 1 month goats under sterile conditions and were digested with collagenase. The cells were collected. Morphological changes and attachment efficiency were constantly observed under phase-contrast microscope. Immunohistochemical staining for type I collagen as well as toluidine blue staining were performed. Ultrastructures of the TMJ cells were observed under transmission electron microscope. Results: Most of the primary fibrochondrocytes presented a short spindle-shape while the rest showed polygon-shape. On the 7th day, the perliferating fibrochondrocytes started to contact each other to form a monolayer covering the bottom of the incubation disc. Immunohistochemical staining of type I and toluidine blue staining exhibited positive results. The fibrochondrocytes cytoplasms were rich in mictochondria and endoplasm reticulum. Conclusion: The fibrochondrcytes isolated from one-month-old goat TMJ disc have good proliferation ability in vitro and cells from passage 1 to 3 might be used as seed cells for TMJ disc tissue engineering.