1.Hepatic T2 value in evaluation of HBV based acute-on-chronic liver failure
Lianjun LAN ; Jian SHU ; Xiaofei LU ; Wen CHEN ; Qin LI
Chinese Journal of Medical Imaging Technology 2017;33(6):902-906
Objective To investigate the value of hepatic T2 value in evaluation of chronic HBV-related acute-on-chronic liver failure (HBV-ACLF).Methods The HBV-ACLF group,chronic hepatitis B group and control group who underwent liver MRI (M-GRASE sequence) were enrolled.The T2 map was produced from the post-processing software,and the mean T2 and R2 value of liver was calculated.The blood biochemical indexes from HBV-ACLF and chronic hepatitis B group were collected in 2 days pre-MR scaning.The differences of T2 and R2 values among 3 groups and the correlation between biochemical indexes and T2 value were analyzed.ROC curve was conducted to evaluate diagnostic efficiency of T2 value for HBV-ACLF.Results There were significant differences of T2value (x2 =19.074,P<0.001) or R2 value (F=10.411,P<0.001) among the 3 groups.The AUC of T2 value for diagnosing HBV-ACLF was 0.86 (P<0.001),with the cut-off value 57.73 ms (R2=0.017).Moderate positive correlation was shown between T2 values and international normalized ratio (INR),prothrombin time (PT),haluronicacid (HA) values (rs =0.65,0.67,0.39,all P<0.05),and moderate negative correlation was shown between T2 values and prothrombin activity (PTA),albumin (ALB),prealbumin (PA) values (rs =-0.67,-0.48,-0.37,all P<0.05).Conclusion T2 or R2 value could reflect the liver function,and were correlated with some biochemical indexes,which illustrated a good diagnostic efficiency for diagnostic of HBV-ACLF.
2.The Metabolism Yield of Cordycepin and Adenosine in Cordyceps militaris by Liquid Culture
Lu WEN ; Hu-Wei SONG ; Jie JIANG ; Chen-Shu YUAN ;
Microbiology 1992;0(03):-
In order to improve the metabolism yield of cordycepin and adenosine,we studied nitrogen sources ,the levels of nitrogen sources and carbon sources,dynamic development in liquid culture ,as well as the total yield of cordycepin and adenosine in culture system through determining the contents of cordycepin and adenosine by HPLC .The results are as follows: not only animal protein but also some plant protein is very good nitrogen sources; in culture solution the suitable levels of nitrogen sources and carbon sources is respectively 3% and 4%; the content of adenosine in culture solution is very low, while it is quite high in mycelia; it is much higher that the total yield of cordycepin in culture solution than in mycelia; when the culture system is oscillated and cultured after 7~9 days, the total yield of cordycepin and adenosine are both high.
3.Small molecular agents against MERS-CoV infection.
Xiao-yun ZENG ; Lu LU ; Shi-bo JIANG ; Shu-wen LIU
Acta Pharmaceutica Sinica 2015;50(12):1520-1526
Middle East respiratory syndrome coronavirus (MERS-CoV) has caused outbreaks of SARS-like disease with 35% case-fatality rate, mainly in the Middle East. A more severe outbreak of MERS occurred recently in the Republic of Korea, where 186 people contracted the infections, causing great concern worldwide. So far, there has been no clinically available drug for the treatment of MERS-CoV infection. The potential drugs against MERS-CoV mainly consist of monoclonal antibodies, peptides and small molecular agents. Small molecular agents have an advantage of easier synthesis, lower cost in production and relatively higher stability. There is better chance for those candidates to gain a quick development. This article reviews the progress of developing small molecular MERS-CoV agents.
Antibodies, Monoclonal
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pharmacology
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Antiviral Agents
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pharmacology
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Coronavirus Infections
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drug therapy
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Drug Design
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Humans
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Middle East Respiratory Syndrome Coronavirus
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drug effects
4.Development of peptidic MERS-CoV entry inhibitors.
Shuai XIA ; Qian WANG ; Shu-wen LIU ; Lu LU ; Shi-bo JIANG
Acta Pharmaceutica Sinica 2015;50(12):1513-1519
In 2012, a new SARS-like coronavirus emerged in the Middle East, namely the Middle East respiratory syndrome coronavirus (MERS-CoV). It has caused outbreaks with high mortality. During infection of target cell, MERS-CoV S protein S1 subunit binds to the cellular receptor (DPP4), and its S2 subunit HR1 and HR2 regions intact with each other to form a stable six-helix bundle to mediate the fusion between virus and target cell membranes. Hence, blocking the process of six-helix bundle formation can effectively inhibit MERS-CoV entry into the target cells. This review focuses on the recent advance in the development of peptidic entry inhibitors targeting the MERS-CoV S2 subunit.
Antiviral Agents
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pharmacology
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Coronavirus Infections
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drug therapy
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Dipeptidyl Peptidase 4
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metabolism
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Drug Design
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Humans
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Middle East Respiratory Syndrome Coronavirus
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drug effects
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physiology
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Peptides
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pharmacology
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Spike Glycoprotein, Coronavirus
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metabolism
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Virus Internalization
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drug effects
5.Study on immunocyte proliferation and DNA damage induced by lead in mice.
Shu-ying DONG ; Chang-qing YING ; Ying WEN ; Ming-jun LU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(6):457-458
Animals
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Cell Proliferation
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drug effects
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Cells, Cultured
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Comet Assay
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DNA Damage
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Dose-Response Relationship, Drug
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Lead
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toxicity
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Lymphocytes
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drug effects
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Male
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Mice
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Mice, Inbred Strains
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Spleen
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cytology
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drug effects
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Thymus Gland
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cytology
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drug effects
6.The automatic optimization arithmetic of selecting beam arcs in X-knife radiotherapy planning system.
Yin-xiang LIU ; Shu-xiang LI ; Qing-wen LU
Chinese Journal of Medical Instrumentation 2002;26(1):20-22
In this paper, two new concepts of DR (Dose Repulsion) and DG (Dose Gravitation) are presented with their calculation formulas. For the problem of selecting beam arcs in x-knife radiotherapy Planning system, a mathematics model of constrained optimization has been built. Furthermore, we have produced a feasible project of automatic selecting optimized beam arcs plan using SA (Simulated Annealing) arithmetic based on the distribution of the fields of DR and DG in the reduced phase space.
Algorithms
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Brain Neoplasms
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radiotherapy
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Models, Theoretical
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Radiotherapy Dosage
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Radiotherapy Planning, Computer-Assisted
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methods
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Radiotherapy, Conformal
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methods
7.Application of the Innovative and Integrative Experiments on Special Skills Training in Food Sciences
Shu-Qing LIU ; Xiao-Lu JIANG ; Hai-Jin MU ; Jin-Xiao FENG ; Wen-Xiao LIU ;
Microbiology 1992;0(03):-
In order to make students have integrative qualities and reform the shortage of former teaching methods in the food microbiology experiment,a new experiment teaching system was established,introducina the integrative and innovative experiment to the students gradually.The reformation of experiment in food microbiology has trained the students well and improved their special skills.Most important of all,it can offer them a chance to realize their own ideas.The students can design an innovative and integrative experiment to carry out their originalities.Good effects have been made since this system is very helpful to improve their consciousness of innovation and integrative abilities in doing experiments.
8.Effects of cluster nursing intervention on dysuria in hospitalized patients undergoing renal biopsy
Xiaoyun LI ; Wen ZHOU ; Jin PENG ; Yang LU ; Qin LIU ; Shu XIONG ; Tao LEI
Chinese Journal of Practical Nursing 2017;33(21):1612-1615
Objective To study the effects of cluster nursing intervention on dysuria in hospitalized patients after renal biopsy. Methods A total of 106 hospitalized patients undergoing renal biopsy during April. 2016 to September. 2016 were divided into control group (50 cases) and experimental group (56 cases) by random number table method. The control group were implemented with traditional methods of care and the experimental group were implemented with cluster nursing intervention.The incidence of dysuria, first average urination time and post-operative urination pattern were compared between two groups. Results The incidence of dysuria in the experimental group was 10.7%(6/56), which was significantly lower than 28.0% (14/50) of the control group (χ2=5.156, P<0.05).The first average urination time of experimental group was (2.95±1.17) hours, which was lower than (5.04±2.27) hours of the control group (t =5.401, P<0.05). The proportion of patients with post-operative self-urination in the experimental group was significantly higher than that in the control group (χ2=6.152, P<0.05). Conclusions Cluster nursing intervention can reduce the incidence of dysuria, shorten the first average urination time, promote post-operative self-urination of patients after renel biopsy and enhance comfort.
9.Effects of advanced glycation end-products on skin keratinocytes by NF-?B activation
Ming TIAN ; Chun QING ; Yi-Wen NIU ; Jiao-Yun DONG ; Shu-Wen JIN ; Fei SONG ; Lan-Nv HUA ; Shu-liang LU
Chinese Journal of Trauma 2003;0(10):-
Objective To investigate the effects of advanced glycation end-products(AGEPs)on the function of normal keratinocytes in vitro so as to explore the role of AGEPs in impaired wound healing. Methods Normal rat keratinocytes were incubated with different concentrations of AGEPs.After 48 hours of culturing,the cell proliferation rates were measured by MTT colorimetric determination.The cell cycle distributions and apoptosis were analyzed with flow cytometry,and the migration was investigated by 24-well fluorimetric cell migration assay kit by exposing to 100?g/ml AGEPs.Nuclear extracts from these cells were examined for binding of nucleotides containing NF-?B consensus by immunocytochemistry and EMSA in vitro.Results The proliferations of normal keratinocytes were significantly arrested and many cells were induced to early apoptosis compared with control ones(P<0.05)by exposing to AGEPs for 48 hours. Meanwhile AGEPs also irritated keratinocytes migration compared with control ones(P<0.05).Inhibiting the activation of NF-?B could partly recover the proliferation of keratinocytes,reverse apoptosis and attenu- ate migration.Conclusion AGEPs are correlated with the migration,proliferation and apoptosis of kera- tinocytes by NF-?B.
10.Validation of an improved Demons deformable registration algorithm and its application in re-contouring in 4D-CT.
Xin ZHEN ; Ling-hong ZHOU ; Wen-ting LU ; Shu-xu ZHANG ; Lu ZHOU
Journal of Southern Medical University 2010;30(12):2619-2624
OBJECTIVETo validate the efficiency and accuracy of an improved Demons deformable registration algorithm and evaluate its application in contour recontouring in 4D-CT.
METHODSTo increase the additional Demons force and reallocate the bilateral forces to accelerate convergent speed, we propose a novel energy function as the similarity measure, and utilize a BFGS method for optimization to avoid specifying the numbers of iteration. Mathematical transformed deformable CT images and home-made deformable phantom were used to validate the accuracy of the improved algorithm, and its effectiveness for contour recontouring was tested.
RESULTSThe improved algorithm showed a relatively high registration accuracy and speed when compared with the classic Demons algorithm and optical flow based method. Visual inspection of the positions and shapes of the deformed contours agreed well with the physician-drawn contours.
CONCLUSIONDeformable registration is a key technique in 4D-CT, and this improved Demons algorithm for contour recontouring can significantly reduce the workload of the physicians. The registration accuracy of this method proves to be sufficient for clinical needs.
Algorithms ; Four-Dimensional Computed Tomography