1.Orbitofrontal cortex action of 5-hydroxytryptamine, glutamate and nitric oxide in an acute forced swimming stress-induced depression model.
Chinese Journal of Applied Physiology 2011;27(4):474-I
Animals
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Depression
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etiology
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metabolism
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Frontal Lobe
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metabolism
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Glutamic Acid
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metabolism
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Male
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Nitric Oxide
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metabolism
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Rats
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Rats, Sprague-Dawley
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Serotonin
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metabolism
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Stress, Physiological
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physiology
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Swimming
3.Case of infertility induced by abortion.
Chinese Acupuncture & Moxibustion 2014;34(11):1072-1072
4.Construction and Identification of Recombinant Human Bcl-2 Adenoviral Vector
Haibing JU ; Zaiquan CHENG ; Zizheng SHU
Journal of Chinese Physician 2001;0(07):-
Objective To construct recombinant human Bcl-2 adenoviral vector. Methods Sense human Bcl-2 cDNA fragment from plasimid pcDNA3 1/bcl-2 was cloned into adenovirus shuttle vector pAdCMV. pAdCMV/bcl-2 and plasmid pJM17 were cotransfected into 293 cells with lipofectamine 2000. Replication defective adenovirus was reproduced and purified. Adenoviral virus DNA was detected with polymerase chain reaction(PCR), and plaque assay was performed to determine the titer of virus. Results Recombinant adenovirus containing bcl-2 target gene was obtained. The titer of virus was 2 0?10 10 pfu/ml. Conclusion High titer of adenovirus containing target gene was successfully obtained by recombinant adenovirus vector.
5.Long-term effect of lamivudine combined with interferon alpha treatment for chronic hepatitis B virus infection.
Dan SHU ; Cheng XU ; Daguo YANG
Chinese Journal of Practical Internal Medicine 2002;0(08):-
Objective To evaluate the efficacy of 2-year treatment of interferon alpha combined with lamivudine therapy in chronic hepatitis B patients. Methods From 1999 to 2001 87 patients were enrolled and randomized into two groups.36 patients received interferon alpha and lamivudine simultaneously for 26 weeks,then lamivudine alone for 76 weeks.51 patients received lamivudine alone for 104 weeks.The efficacy was evaluated by biochemical and virological parameters.The YMDD motif was detected in both groups. Results There was no difference between two groups in HBV DNA response ( P =0.24).Proportion of HBeAg/Anti-HBe seroconversion in combination group was significantly higher than lamivudine group (38.9% vs 17.6%)( P =0.03).HBV YMDD mutation rate in combination group was lower than in lamivudine group (22.2% vs 43.1%, P =0.04). Conclusion The efficacy of combination therapy appeares to be better than lamivudine monotherapy.Combination treatment may delay or diminish the development of HBV YMDD mutation.
6.Competing endogenous RNA and tumor pathological mechanism
Shu ZHOU ; Na SHEN ; Fanjun CHENG
Journal of International Oncology 2017;44(2):115-117
Competing endogenous RNA (ceRNA) is a class of RNA which includes mRNA,pseudogenes,long non-coding RNA (lncRNA),circular RNA (circRNA).ceRNA weakens its inhibitory effect on mRNA translation through competitive binding with shared microRNA (miRNA).Many studies have confirmed that the disorder of ceRNA is closely related to the occur-ence of breast cancer,gastric cancer,lymphoma and other tumors.With the improvement of researches,ceRNA may be used as a tumor marker of clinical diagnosis and therapeutic target.
8.Advances in UL7 gene of herpesvirus.
Jie HUANG ; An-Chun CHENG ; Ming-Shu WANG
Chinese Journal of Virology 2011;27(5):501-504
Animals
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Herpesviridae
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genetics
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metabolism
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Humans
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Viral Proteins
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chemistry
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genetics
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metabolism
10.Recent progress in short QT syndrome.
Kui HONG ; Hai SU ; Xiao-shu CHENG
Chinese Journal of Cardiology 2006;34(9):858-860
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Arrhythmias, Cardiac
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diagnosis
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genetics
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therapy
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Child
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Child, Preschool
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Electrocardiography
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Humans
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Infant
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Male
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Middle Aged
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Syndrome