Conjunctiva ; surgery ; Diagnosis, Differential ; Eye Neoplasms ; metabolism ; pathology ; surgery ; Humans ; Infant ; Male ; Neoplasm Recurrence, Local ; Nerve Sheath Neoplasms ; metabolism ; pathology ; surgery ; Reoperation ; S100 Proteins ; metabolism

ObjectiveTo observe the pathogenic relationship and the mechanisms between the first molars and the temporomandibular disorders(TMD) by getting epidemiology data.MethodsThe oral examinational people during 2007 to 2011 as investigating objects were selected and epidemiological study was carried out with standard clinical diagnostic criteria for dental caries TMD.ResultsThe people with dysfunctional first molars showed that TMD positive rate was 51.48％,and normal molars was 23.47％.The results had significant difference( x2 =54.34,P ＜ 0.01 ).ConclusionThe people with dvsfunction of first molars mav be liable to TMD.

Objective To construct recombinant prokaryotic plasmid DNA encoding mycobacterium tuberculosis (TB) heat shock protein 70 (HSP70) - Dnak gene obtained from pMT70, then purify its characterizes and the role of rHSP70 in tumor therapy. Methods Amplified by polymerase chain reaction (PCR) and with terminal modification, the Dnak gene was cloned into the vector with T7 promoter and was confirmed by restriction endonuclease digestion. So a new plasmid pMT70 - 3 was constructed and transformed in E. Coli. Strain BL21DE3. The engineering bacteria were induced by IPTG. Western-blot,natine-page and scanning were used to analyze the results. The human HSP70 were induced with peptides of lymphocytic leukemia cells of L1210 of mice in binding buffer which contain MgCl2 and ADP at 37 ℃ , then mice were given rHSP70 peptide - complex by SV injection twice at weekly intervals. Results The Dnak gene could be highly expressed in E. Coli as soluble protein. The expressing efficiency was 65% of the total cell protein and the soluble protein was 90% of the expressed protein. The rHSP70 - peptide complex could improve the immune protective function which had significant difference compared with controls (P

Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Female ; Hemangioblastoma ; metabolism ; pathology ; Humans ; Ki-67 Antigen ; metabolism ; Meningeal Neoplasms ; metabolism ; pathology ; surgery ; Meningioma ; metabolism ; pathology ; surgery ; Middle Aged ; Mucin-1 ; metabolism ; Neoplasm Recurrence, Local ; Vimentin ; metabolism

Objective To determine the correlation of plasma brain natriuretic peptide(BNP) with severe degree of dilated cardiomyopathy in children.Methods Thirty children with dilated cardiomyopathy and 30 healthy subjects were selected in this degree of study, plasma BNP concentration was measured and compared among groups by using t test.Correlation of BNP levels with left ventricular ejection fraction and heart function was investigated using linear regression analysis.Results Children with dilated cardiomyopathy had significantly higher mean BNP levels compared with healthy children [(429.4?270.2) ng/L vs (67.0?10.2) ng/L].Significantly positive correlations were found between BNP and heart classification(r=0.950 P

Aluminum ; toxicity ; Animals ; Animals, Newborn ; Calcium ; metabolism ; Cerebral Cortex ; drug effects ; metabolism ; Dose-Response Relationship, Drug ; Female ; Male ; Rats ; Rats, Wistar

OBJECTIVETo evaluate the role of RNA interference (RNAi) in silencing the enhanced green fluorescent protein (eGFP) expression in 293T and Mel cells.

METHODSNested-PCR was used to amplify H1 promoter from human 293T cells for driving RNAi synthesis. RNAi vectors (TR1) for silencing the eGFP expression was constructed. The eGFP vector and RNAi vector (TR1) were then co-transfected into the 293T and Mel cells, in which the silencing effect on eGFP expression was investigated by fluorescence microscopy, reverse transcription-PCR(RT-PCR), fluorescence-assited cell sorting(FACS) analysis and real-time RT-PCR.

RESULTSRNAi could effectively reduce more than 50 percent of eGFP expression in 293T cells as well as in Mel cells.

CONCLUSIONThe RNAi vector constructed in this way paper can effectively inhibit eGFP expression in cells.

Cell Line ; Flow Cytometry ; Genetic Vectors ; genetics ; Green Fluorescent Proteins ; genetics ; Humans ; RNA Interference ; Reverse Transcriptase Polymerase Chain Reaction

Objective To investigate the intima-media thickness and cardiac remodeling in patients with prehypertension and impaired glucose tolerance.Method Three-hundred patients were divided into four groups:normal control ( NC,n = 61 ),prehypertension ( PH,n = 83 ),impaired glucose tolerance ( IGT,n = 91 ),prehypertension and impaired glucose tolerance ( PH + IGT,n = 65 ).Intima-media thickness (IMT) was measured by doppler ultrasonography.Left ventricle mess (LVMI),midwall fractional shortening (mFS),peakE/peakA ( E/A ) were measured by echocardiography.Results ( 1 ) IMT was significantly increased in PH,IGT and PH + IGT groups than in NC group [ (0.7 ± 0.1 ) mm,(0.7 ± 0.1 )mm and( 1.0 ±0.1 ) mm vs.(0.6 ±0.1 ) mm,all P ＜0.01 ],which was significantly high in PH + IGT group than in PH or IGT group.Regression analysis demonstrated that high-sensitivity C-reactive protein (hsCRP),2-hour postprandial blood glucose (2hPBG),systolic pressure (SBP),diastolic blood pressure (DBP) were independent predictors to increased IMT.(2) LVMI was higher in PH and PH + IGT groups than in NC group [ (97.0 ± 3.3 ) g/m2,(97.1 ± 2.8 ) g/m2 vs.( 87.0 ± 2.0) g/m2,( 87.9 ± 1.5 ) g/m2,all P ＜ 0.01 ],and positively related with SBP,DBP.( 3 ) mFS was lower in PH and PH + IGT groups [(14.0±0.8)％,(14.0±0.8)％ vs.(18.3±1.0)％,(18.2±0.5)％,P＜0.01],negatively related with SBP and DBP.E/A was similar among groups and lower E/A was associated with higher SBP.Conclusion Vascular and left ventricular structure remodeling and systolic dysfunction could be detected in prehypertension patients.Impaired glucose tolerance could enhance vascular remodeling in prehypertension patients.

OBJECTIVETo study role of dental follicle in tooth root development.

METHODSSixteen mandibular first molar dental germs from eight five-day postnatal Balb/c mice were divided into two groups randomly. Dental follicle of germs in one group was undetached and that of another group was removed. Subsequently, each of the germs was separately transplanted to back-muscles of adult nude mice. At seventh and fourteenth day after transplanting, the germs were collected, fixed, demineralized, dehydrated, and embedded in wax in sequence. Serial sections of 5 microm thick were made following the routine methods, stained with haematoxylin-eosin dying solution, and observed under a light microscope.

RESULTSAll implantations were located in the back-muscles with abundant capillary vasculature. Under microscope, although all tooth germs could further develop after grafting, tooth germs without dental follicle developed slowly with small size and low calcification compared to those with dental follicle. Although position of Hertwig's epithelial root sheath of all germs seemed no changing, roots of the group with dental follicle could further develop and the roots develop toward the apical direction; this tendency couldn't be seen in the germs of another group. Inflammatory cells could be seen in and out of the pulp cavity of the two groups at 7th day after grafting, while no obvious inflammatory cell was observed at 14th day after grafting.

CONCLUSIONDental follicle play an important role in tooth root development. It probably can lead tooth root to develop in normal direction.

Animals ; Dental Pulp Cavity ; Dental Sac ; Enamel Organ ; Mice ; Mice, Nude ; Molar ; Odontogenesis ; Tooth ; Tooth Germ ; Tooth Root

Objective To investigate the expression of peroxisome proliferators-activated receptor ? (PPAR?) in trophoblast and relation between PPAR? ligands and trophoblast invasion.Methods We examined the expression of PPAR? by immunohistochemistry,immunocytochemistry and real time quantitative PCR.We next examined,using the cytotrophoblast culture model,the biological role of PPAR? ligands in vitro.Results PPAR? was mainly localized in the nuclei of villous cytotrophoblast and extravillous cytotrophoblast of cell islands and cell columns.In villous tissue and cultured trophoblast from early first trimester,the level of expression of PPAR? mRNA and protein was 36.0?5.1,13.4?3.1 and 1.35?0.08,1.13?0.11;from late first trimester it was 23.3?5.5,6.1?1.3 and 1.17?0.03,0.86 ?0.05,and the expression of PPAR? was obviously decreased (P