1.How to elevate theory teaching effect in medical immunology
Chinese Journal of Medical Education Research 2006;0(08):-
The theory of Medical Immunology is independent,which includes a lot of new knowledge.Optimizing resource of medical immunology,recombinating knowledge structure,building knowledge framework and leading in PBL(Problem Based Learning) teaching method help to arouse students’studying interest and elevate teaching effect.
2. Percutaneous thermal ablation for nodular thyroid diseases: An assessment of short-term effects
Academic Journal of Second Military Medical University 2011;32(12):1316-1320
Objective To investigate the short-term effects of ultrasound-guided percutaneous microwave ablation (MWA) and radiofrequency ablation (RFA) for treatment of thyroid nodules. Methods Totally 104 patients with 289 thyroid nodules underwent ultrasound-guided internal-cooled MWA and bipolar RFA in our department from July 2009 to January 2011. The outcomes of patients were closely assessed for a short-term of 6 months; the thermal ablation-related side-effects and complications were also discussed. Results Focal ultrasound images of the 289 thyroid nodules and the core-biopsy pathology findings of some nodules revealed complete coagulative degeneration of the ablated lesions. Follow-up for 3-6 months showed that sizes of all the nodules decreased to different extents. Only one nodule needed an extra ablation. No severe complications were observed. Conclusion The ultrasound-guided percutaneous MWA and RFA are effective, minimally invasive, easy to perform and can achieve rapid recovery. The methods can be used for out-patients with nodular thyroid diseases.
3.Analysis of IL-8 secretion in THP-1 cells stimulated by lipopolysaccharide and capsular polyssacharide extracted from Porphyromonas gingivalis
Yiwei WANG ; Dali LIU ; Rong SHU
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(4):458-461
Objective·To isolate lipopolysaccharide (LPS) and capsular polysaccharide (CPS) from Porphyromonas gingivalis (Pg) and analyze their ability to induce THP-1 cells to secrete interleukin 8 (IL-8) in vitro. Methods·LPS and CPS were isolated from the polysaccharides by gel filtration chromatography, which had already been extracted from Pg ATCC33277 and SJD4. The isolated LPS and CPS from different strains were used to stimulate THP-1 cells in different doses and for different periods. The level of IL-8 in the cell culture suspension was measured by ELISA. Results·LPS and CPS were successfully separated from the polysaccharides in Pg ATCC33277 and SJD4. The relative molecular masses of LPS and CPS were 20000-50000 and 150000-500000, respectively. LPS and CPS could induce THP-1 cells to produce IL-8 in vitro in a time- and dose-dependent manner. The effect of LPS from ATCC33277 on secretion of IL-8 was greater than that of CPS with the dose of 0.1 μg/mL (P<0.05). However, the difference could only be found at 24 h in SJD4, and the effect of CPS was greater (P<0.05). Conclusion·As a component of polysaccharides extracted from Pg, CPS can induce THP-1 cells to secrete IL-8 in vitro in a time- and dose-dependent manner.
4.Progress on the measurement of corneal biomechanical properties
Qian, WANG ; Lin-Lin, WANG ; Yan, ZHANG ; Shu-Rong, WANG
International Eye Science 2016;16(10):1840-1846
Cornea is the major refractive components of the eye. As a viscoelastic tissue, cornea exhibits complicated biomechanical properties: non - linear elasticity, anisotropy and viscoelasticity. The biomechanical properties play an important role in keeping the normal structureand function. Changes in biomechanical properties are always earlier than the clinical symptoms. So quantitative measurement of the biomechanical properties benefits the early diagnosis and treatment of diseases. Different methods to measure the biomechanical properties of cornea were reviewed in detail, including classic ex vivo destructive tests, commercially available in vivo measuring methods and other emerging methods with the potential for clinical application but not validated for in vivo measurement. The operating principles, advantages as well as limitations of these methods were also described.
7."The comprehensive reform research and practice of basic medical teaching based on the basis of""student-centered"""
Qiang WANG ; Rong SHU ; Jing LI ; Jing LIU ; Linguo WU
Chinese Journal of Medical Education Research 2015;(8):783-786
The development trend of medical education is comprehensive, which is character-ized by breaking the boundaries among different disciplines, constructing a main line centering on or-gan and system, and implementing student-centered problem-based learning. This paper analyzed the main problems of basic medical education in Wuhan University of Science and Technology, such as insufficient teaching reform to promoting students' autonomous learning and failed to create excellent teaching and research group et al. Also, the paper proposed comprehensive reform measures such as integrating teaching units , establishing Teacher Education Development Center, implementingCourse director andTeam leader system, promoting teaching model ofstudent centered and in-tegrating curriculums of basic medicine to establish course model centering on the organ and system et al, with some success.
8.Clinical effects of photo-activated disinfection as an adjunctive therapy for the treatment of chronic periodontitis
Shucheng HU ; Rong SHU ; Zhongchen SONG ; Mengjun SUN ; Yiwei WANG
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(5):621-626
Objective · To investigate the clinical effects of photo-activated disinfection (PAD)-assisted non-surgical therapy on the treatment of chronic periodontitis. Methods · Eleven patients with chronic periodontitis (totally 118 selected sites) were randomly enrolled. A split-mouth self-control method was adopted. Selected sites with the probing depth (PD) of bilateral homonymous teeth ≥ 5 mm were randomly assigned to the group A (receiving scaling and root planning, SRP) and the group B (receiving SRP+ PAD). Periodontal clinical indexes, inflammation indexes, and periodontal pathogens at selected sites were examined at baseline and 6 and 12 weeks after treatment. Results · Both SRP alone and SRP+PAD improved the periodontal inflammation significantly. The positive rate of bleeding on probing (BOP) 12 weeks after treatment was remarkably lower in the group B than in the group A (37.3% vs 57.6%, P=0.042). Both SRP and SRP+PAD effectively decreased matrix metalloproteinase-8 (MMP-8) and interleukin-1β (IL-1β) levels in gingival crevicular fluid. The IL-1β levels 6 and 12 weeks after treatment were significantly lower in the group B than in the group A [(17.99±14.26) pg/mL vs (32.64±30.69) pg/mL, P=0.007; (11.37±10.25) pg/mL vs (23.85±15.55) pg/mL, P=0.000]. Both treatments decreased the ratio of P. gingivalis and F.nucleatum in total bacteria. Conclusion · PAD can serve as a potential adjunctive therapy to conventional SRP in the treatment of chronic periodontitis with better improvement of bleeding gums and control of periodontal inflammation.
9.Analysis of 3-year survival of implants in patients with chronic periodontitis
Jielei QIAN ; Rong SHU ; Zhongchen SONG ; Yufeng XIE ; Yiwei WANG
Journal of Shanghai Jiaotong University(Medical Science) 2017;37(3):368-372
Objective · To observe short and medium term survival of implants in patients with chronic periodontitis after implantation. Methods · 54 patients with chronic periodontitis (133 implants) were enrolled from August 2011 to August 2013. The survival of implants was observed and the periimplant diseases were compared and analyzed between patients with different degrees of chronic periodontitis. Results · The 3-year survival rate of implants was 97.74%. The differences between patients with different degrees of chronic periodontitis were not statistically significant (P=0.452). Periodontal pocket depth (PPD) and modified plaque index (mPLI) were significantly higher in patients with severe chronic periodontitis than in patients with mild and moderate chronic periodontitis. For patients not receiving supportive periodontal therapy (SPT), the peri-implantitis rate in patients with severe chronic periodontitis was significantly higher than that in patients with mild and moderate chronic periodontitis (P=0.009). For smokers, the periimplantitis rate in patients with severe chronic periodontitis was significantly higher than that in patients with mild and moderate chronic periodontitis (P=0.016). Conclusion · For patients with chronic periodontitis, the theraputic effect of implant treatment is good. Plaque control, SPT, and smoking cessation can reduce the incidence of peri-implantitis.
10.Effects of compound Daqiqi decoction combined cisplatin on Bcl-2/Bax expression of nude mice ovarian cancer subcutaneous transplanted tumor.
Yang-bojun YANG ; Shuang WANG ; Shu LI ; Rong CHEN
China Journal of Chinese Materia Medica 2015;40(8):1575-1579
OBJECTIVETo determine the compound Daqiqi decoction( CDQD) combined cisplatin plays on the cell apoptosis of subcutaneous transplanted ovarian cancer in nude mice, to provide theory evidence for clinical treatment.
METHODMaking the models of subcutaneous transplanted ovarian cancer in nude mice, and divide the 40 mice with tumor into 5 groups (n = 8), the model control group, CDQD low dosed group, CDQD high dosed group, cisplatin group, cisplatin combined CDQD group, killing all the mice after 3 weeks' treatment and stripping tumors. Measure the volume and weight of the tumor and calculate tumor growth curve damps, the inhibition rate. Examining the expression of the Bcl-2(B-cell leukemia /lymphoma 2) and Bax (Bcl-2 associated x protein) mRNA and protein by the RT-PCR and the Western blot.
RESULT(O)The tumor weight shows that there was certain lighter effect in each CDQD group, and compared with cisplatin groups has no statistical significance, the cisplatin combined CDQD group is obviously lighter than that of the other group(P <0.01). The tumor growth curve damps shows that compared with model control group, the treatment group tumors had some extent narrowing (P < 0.01). (2) RT-PCR results: Bax (Bcl-2 associated x protein) mRNA expression shows that compared with model control group, the treatment group has increased (P < 0.01), and the cisplatin combined CDQD group compared with the other group is the highest, there was significant difference with the rest of the treatment group (P < 0.01). In the Bcl-2 mRNA express lowest in cisplatin combined CDQD group (P < 0.01), there has no difference between CDQD high dose group and cisplatin group. (Western blot shows: compared with model control group, the Bax protein of treatment group has increased expression (P < 0.01), the expression of Bax protein in cisplatin combined CDQD group is the highest(P < 0.01). There has no difference between CDQD high dose group and cisplatin group. The Bcl-2 protein expression of the cisplatin combined CDQD group is the lowest (P < 0.01), there has no difference between CDQD high dose group and cisplatin group.
CONCLUSIONThe effect of CDQD on subcutaneous transplantation ovarian tumor has promoting apoptosis function, its mechanism may be related to downgrade the Bcl-2 expression, higher expression of bax, stimulation on the apoptosis of tumor cells; cisplatin combined CDQD have synergistic effect.
Animals ; Cell Line, Tumor ; Cisplatin ; administration & dosage ; Disease Models, Animal ; Drug Therapy, Combination ; Female ; Humans ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Ovarian Neoplasms ; drug therapy ; genetics ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; bcl-2-Associated X Protein ; genetics ; metabolism