Anal Canal/surgery* ; Anorectal Malformations ; Constriction ; Fecal Incontinence ; Humans ; Rectal Prolapse

Objective: The current study was designed to distinguish Phase G0/G1， S， G2/M cell groups in the whole cell cycle by DNA flow cytometry. Method: The authors developed a multiparameter flow cytometry of cyclin E＋ A/DNA for MOLT-4. Result: The data showed that the method could distinguish Phase G0, early G1, late G1, S, G2,and M cells. The method could be used in molecular cell biology, especially in cell kinetic study.

OBJECTIVETo study the effect of crocin on rat experimental hyperlipemia and its mechanisms.

METHODHyperlipemia model was established by feeding heavy cholesterol for 2 months and the effect of crocin on blood lipid in experimental hyperlipemia rats was observed. Aortic smooth muscle cells were cultured in different culture media and proliferation was measured by MTT assay. Western blotting was used to detect the effect of crocin on phosphorylation of p38 MAPK.

RESULTCrocin not only decreased greatly the content of cholesterol, triglyceride and density lipoprotein in blood, but also increased the content of high density lipoprotein. In addition, the proliferation of smooth muscle cells and the activation of p38MAPK were inhibited by Crocin.

CONCLUSIONCrocin prevents atherosclerosis in hyperlipemia, which may be mediated by the inhibition of both proliferation of smooth muscle cells and activation of p38MAPK.

Animals ; Aorta, Thoracic ; cytology ; Carotenoids ; isolation & purification ; pharmacology ; Cell Proliferation ; drug effects ; Cholesterol ; blood ; Crocus ; chemistry ; Hyperlipidemias ; etiology ; metabolism ; pathology ; Hypolipidemic Agents ; pharmacology ; Lipoproteins, HDL ; blood ; Lipoproteins, LDL ; blood ; Male ; Myocytes, Smooth Muscle ; cytology ; enzymology ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley ; Triglycerides ; blood ; p38 Mitogen-Activated Protein Kinases ; metabolism

OBJECTIVETo study the clinicopathologic features, diagnosis and differential diagnosis of myositis ossificans (MO).

METHODSThe clinical features, radiologic results and pathologic findings of 15 cases of MO (including biopsy and surgical specimens) were analyzed. The hematoxylin and eosin sections were reviewed under light microscope. Immunohistochemical staining for S-100 protein, vimentin, desmin, actin and osteonectin was performed.

RESULTSThe age of the patients ranged from 12 to 46 years. The male-to-female ratio was 11:4. Thirteen cases were located in the parosteum of long bone or subperiosteal soft tissue. The remaining two cases occurred in iliac region and palm, respectively. Five patients had history of injury, while 2 patients had operation before. Four patients had no history of trauma and the remaining one had unknown clinical history. Histologically, zonation pattern was not conspicuous in 10 biopsy cases and 8 corresponding surgical specimens. On the other hand, zonation pattern was observed in 5 biopsy cases and 7 corresponding surgical specimens. Follow up revealed relapses in two patients. Immunohistochemical study showed various degree of positivity for vimentin, desmin, actin and osteonectin. S-100 protein was focally positive in 2 of the cases. The Ki-67 index varied from 1% to 10%.

CONCLUSIONCorrect diagnosis of MO relies on correlation of clinical features, radiologic examination and pathologic findings.

Adolescent ; Adult ; Biopsy ; Child ; Female ; Humans ; Male ; Middle Aged ; Myositis Ossificans ; diagnosis ; genetics ; pathology ; S100 Proteins ; genetics ; Vimentin ; X-Rays ; Young Adult

OBJECTIVETo study the clinicopathologic features, diagnosis and differential diagnosis of malignancies in giant cell tumor (MGCT).

METHODSThe clinicopathologic features of 13 cases of MGCT were retrospectively reviewed.

RESULTSThirteen cases of MGCT were found amongst a total of 603 cases of giant cell tumor encountered. Six of the 13 cases represented concurrent malignancy in giant cell tumor while the remaining 7 cases was malignant transformation in recurrent giant cell tumor. The age of the patients ranged from 21 to 71 years (mean age = 39.5 years) in the first group and from 27 to 52 years (mean age = 36.7 years) in the second group. In concurrent MGCT, a high-grade sarcoma component was present in conjunction with the giant cell tumor component. In malignant transformation of recurrent giant cell tumor, the original tumor was giant cell tumor and the recurrence showed features reminiscent of malignant fibrous histiocytoma.

CONCLUSIONSThe diagnosis of malignancies in giant cell tumor requires correlation of clinical, radiologic and pathologic features. The entities need to be distinguished from other giant cell-rich tumors including primary malignant fibrous histiocytoma and giant cell osteosarcoma.

Adult ; Aged ; Bone Neoplasms ; diagnostic imaging ; pathology ; surgery ; Cell Transformation, Neoplastic ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Giant Cell Tumor of Bone ; diagnostic imaging ; pathology ; surgery ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Neoplasms, Second Primary ; pathology ; Osteosarcoma ; pathology ; Radiography ; Sarcoma ; pathology ; Young Adult

OBJECTIVETo study the cost-effectiveness, benefit and utility of infant hepatitis B vaccination in Shanghai from 1992 to 2001.

METHODSTo calculate the cost of hepatitis B vaccination by cost analysis method. Both the numbers of persons with HBsAg positive and patient with hepatitis B, cirrhosis and liver cancer decreased as the index of direct effect. To study the sick-time and the cost of treating hepatitis B, cirrhosis and liver cancer patients, a face to face questionnaire was used and quasi method was adopted to understand the effect of cure and the course of hepatitis B. The cost benefit analysis method was also used to calculate the cost benefit of HBV vaccine. The disability adjusted life years (DALY) was regarded as an index of utility to measure the disease burden.

RESULTSInput of 501,129.49 Yuan might have the result of reducing one liver cancer patient, ten cirrhosis patients, one hundred chronic hepatitis B patients and one thousand HBsAg positive people. The cost of hepatitis B vaccination was 0.24 hundred million Yuan during the past ten years in Shanghai, which had obtained the total benefit value of 41.22 hundred million Yuan, with a cost benefit ratio of 1:172 Yuan. It was estimated that the total disease burden of hepatitis B, cirrhosis and liver cancer patients was 59,762.55 DALY in order to reduce one DALY loss cost of 402.50 Yuan.

CONCLUSIONHBV vaccine inoculation in infants seemed to be a low-cost input and high-effect output strategy.

Adult ; Cost-Benefit Analysis ; Female ; Hepatitis B ; complications ; economics ; prevention & control ; Hepatitis B Vaccines ; economics ; therapeutic use ; Humans ; Immunization Programs ; economics ; Infant, Newborn ; Liver Cirrhosis ; economics ; etiology ; Liver Neoplasms ; economics ; etiology ; Male ; Markov Chains ; National Health Programs ; Quality of Life ; Surveys and Questionnaires ; Vaccination

Objective To study the correlation between the method Strengthening Earth to Generate Metal ( SEGM ) on infantile repeated re-spiratory infection ( RRTI) of lung and spleen qi deficiency and the annual incidence of respiratory tract as well as the correlated immune response.Methods After the case screening , the selected patients were divide into 4 groups: control group (17 cases, no drugs), Chinese medicine group (36 cases, Shenling Baizhu particles , 1.5 -3 g each time, 2 times a day), western medicine group (36 cases, pidotimod granules, 0.4 g each time, 2 times a day) and integrated group of traditional and western medi-cine (37 cases, Shenling Baizhu granule plus Pidotimod granules ).The changes of children′s annual incidence and the immune index before and after treatment for 8 weeks were observed.Results The method of strengthening earth to generate metal can reduce the annual incidence of repeated respiratory tract infection ( P<0.05 ) , and can regulate the T lymphocyte subpopulation homeostasis , with serum immunoglobulin levels elevated (P<0.05).Conclusion The method of SEGM can reduce the annual incidence in chlid patients with recurrent respiratory tract infection of lung and spleen qi deficiency ( P<0.05);and regulate the immunity of the patients.

Objective To research the effects of chronic suppurative otitis media on bone conduction threshold in old patients. Methods The files of patients with unilateral chronic otitis media were retrospective analyzed, who were all oder than 60 years, who were inpatient in our department since January 2005 to March 2009. Conventional puretone audiometry test was carried out. Bone conduction thresholds were calculated for frequencies of 0. 5, 1,2, and 4 kHz, with comparison between the ear with chronic otitis media and controlateral ear. Thresholds were examined separately for each frequency. Results The bone conduction threshold for the normal side was lower than those for the ear with chronic otitis media. The threshold shift was statistically significant for each frequency(P ＜0. 01 ). There were no diverences between the groups when analyzed for the presence of cholesteatoma except at 2 kHz frequencies ( Z = - 1. 975,P = 0. 048). There were diverences between the groups when analyzed for an interruption of the ossicular chain only at 2 kHz frequencies ( Z = - 2. 721, P = 0. 007 ). There were diverences between the groups when the duration of middle ear disease was not same at 1 kHz and 2 kHz frequencies( Z value were - 2. 877,-2. 624, P＜0. 01, respectively). Conclusions This study shows that chronic otitis media can enhance bone conduction threshold for old patients. All measures for early cure should be considered as early as possible in oder patients with chronic otitis media to prevent advance of sensorineural hearing loss.

Objective To explore the situation and influential factors of the reemployment of patients with occupational hand injuries and to provide a theoretical basis for targeted intervention.Methods Totals of 1472 cases of patients with occupational had injuries from January 2010 to December 2011 were included in this study.1326 patients who finished effective investigations were divided in the reemployed group and the unreemployed group.Single factor analysis and Logistic regression analysis were used to analyze data of both groups.Results A Total of 1071 out of 1326 patients with occupational hand injuries got reemployed within 8 months,accounting for 80.77％,and the average absence time was 55 (8.0,142.5) days.Patients with different ages,mobility,educational levels,company types,occupational duties,injury types,medical payments and rehabilitation treatments had different rate of reemployment,and the differences were statistically significant (x2 =244.196,62.103,50.341,13.331,10.944,170.741,80.503,71.908,respectively; P ＜0.05).And there was no statistically significant difference of reemployment rate between patients with different genders and marriage status (P ＞ 0.05).Logistic regression analysis showed that age,mobility,education level,injury type,medical payment and rehabilitation treatment were the most influencing factors to their reemployment (P ＜0.01).Conclusions The reemployment status of patients with occupational hand injuries is not good,and there are many influencing factors.It is suggested that social support should be strengthened,and extended nursing should be implemented according to their rehabilitation demands,so as to promote their reemployment.