Objective To explore influential factors and features of relapse of presenting with an initial episode of acute central nervous system inflammatory demyelinating disease(IDDCNS) in children.Methods By group-control study,20 relapsing patients with IDDCNS compared with 50 single episoding patients with IDDCNS whose case history longer than 0.5 year,whose age at onset,gender,preceding infection or immunization,neurologic syndromes at presentation,therapeutic response,and final neurologic outcome after initial episode were analyzed by mono-factor analysis and multi-factor nonconditional Logistic regression model.Results 1.The risk of relapse increased in the near future,75.0% relapsed before 6 months.There were 18 cases diagnosed multiple sclerosis(MS),1 case neurooptic myelitis and 1 case immaturity myelitis in relapsing patients.There were 39 cases diagnosed acute disseminated encephalomyelitis in single episoding patients.2.Mono-factor analysis:8 factors were not statistically significant,including age at onset,gender,seizure,palsy,conscious disturbance,ataxia,natural relief,sequelae after the first attack;6 factors were statistically significant,including infectious or vaccination agents before episode,fever,visual disorder,multiple neurological signs,positive pathologic reflex,with marked improvement to cortical hormone.3.In the multi-factor stepwise regression analysis,visual disorder(OR=42.50)and positive pathologic reflex(OR=8.666)were the independent influencing factors,multiple neurological signs(OR=0.068)was independent protecting factor.Conclusions The rate of relapse in IDDCNS is lower than abroad and most relapse before 0.5 year,the main diagnosis of relapsing case is MS.When visual disorder and positive pathologic reflex presenting in the fist episode of IDDCNS,the opportunity of relapse will increase,and will decrease when multiple neurological signs presenting.

Objective To observe the therapeutic effect of the method of soothing liver qi stagnation and invigorating blood circula- tion to remove stasis for depression.Methods Totally 90 depression cases were randomized into treatment group and control group with 45 in each.The treatment group was given modified Xiaoyao Powder(Powder for liver qi stagnation with blood stasis) based on the principle of soothing liver qi stagnation to remove stasis.The control group was given Fluoxetine.The course of treatment was 8 weeks in all.Self-rating Depression Scale(SDS),Hamilton Depression Scale(HAMD),Treatment Emergent Symptom Scale (TESS) and scores of TCM symptoms before and after treatment were recorded for comparison.Results SDS,HAMD,TESS and TCM symptoms were improved after treatment in the treatment group(P

Event-Related Potentials, P300 ; physiology ; Humans ; Individuality ; Male ; Memory, Short-Term ; physiology ; Neuropsychological Tests ; standards ; Young Adult

Objective To investigate the action and the mechanism of Jinyin granule(JYG) on cholecystitis in guinea pigs.Methods Cholecystitis models of Guinea pigs were established and then randomly divided into six groups: normal group,model group,XiaoYanLiDan tablet(XYLDT) group,treatment groups including low,middle and high dosage groups of JYG.The Pigs were given above drugs respectively while in normal and model groups the same volume of normal saline was given.Body weight,gallbladder weight,bile amount,IL-1? in the serum were measured and gallbladder pathological tissue was observed by light microscopy.Results Comparing with the model group,the treatment groups had higher body weight,lower gallbladder weight,less bile amount,obviously slighter inflammation and less amount of IL-1? in the serum.Conclusion JYG possesses the therapeutic action to cholecystitis on guinea pigs and its mechanism is interrelated to the decreasing of IL-1? in the serum.

Objective To explore the application of question-based lectures made by students in teaching chapter of nuclear chemical and biological weapon damage among field military nursing.Methods The participants were junior students of four-year undergraduate program.Questions on emergency treatment and care of chemical and biological weapon damage were proposed after a lecture by teacher.Students were divided into three groups after class and each group was assigned to a lecture on emergency treatment and care of one kind of weapon damage.Students had to collect information and prepare PPT for the next lecture.After lectures given by students at next class,discussion on the lectures was organized and comments were given by teachers.Results All students approved this kind of teaching activities,97.44％ believed that it can arouse their interest in study,94.87％ participated in the collection of data,89.74％ took part in the discussion,and 87.18％ obtained the sense of achievements.Conclusion Question-based lectures made by students are helpful in stimulating their interest in learning,cultivating their abilities of acquiring and applying knowledge.The teaching activities achieve the preferable results through proper application and meticulous organization.

AIM: To investigate the pathological changes of motoneuron's function and morphology after root avulsion in order to study the neurobiology mechanism of motoneuron death. METHODS: Twenty female adult Sprague-Dawley rats, 200-300 g were used. The C 5-C 8, T 1 roots of the right brachial plexus were avulsed. All rats were killed 3 d, 5 d or 1 week after avulsion. One group of the C 5-C 8 spinal cords freeze sections (40 ?m thick) were collected for the NADPH-d histochemistry with neural red counterstained. Another group of the C 5-C 8 spinal cords freeze sections (40 ?m thick) were collected for the c-Jun immunocytochemistry stain. The paraffin sections (5 ?m thick) were collected for HE stain. The amount of NOS-positive and survival motoneurons was counted. The percentage of NOS expression and motoneuron survive were quantitatively analyzed considering the amount of contra lateral motoneurons as one hundred percent. RESULTS: The NOS expression rate was 0.74%?0.59% (3 d), 24.84%?6.73%(5 d), or 51.16%?8.67% (1 week), respectively. The survive rate was 93.00%?4.32% (3 d), 93.67%?5.27% (5 d), or 89.83%?2.65% (1 week), respectively. The motoneuron expressed c-Jun as early as 3 days after avulsion. The expression declined afterward until one week after avulsion. There was no significant change on the size of motoneuron. The nuclear membrane was still clear but some nuclei were not located in the middle of the cell body. There were some nucleoli with the chromatin condensation. CONCLUSION: The motoneuron NOS expression and cell death were increased within one week after spinal root avulsion. meanwhile, the c-jun expression was decreased. The NO/NOS may induce the motoneuron death by inhibiting the regenerating reactions of motoneuron after root avulsion injury.

Objective To investigate the influence of indomethacin to the unite treatment effect on chronic phase myeloid leukemia (CML-CP) with interferon alpha-2b (IFNα-2b) and low dose cytarabine (LD-Ara-C).Methods 22 CML-CP patients were randomly divided into two groups.Control groups (10 cases) injected with IFNα-2b (3 million units),injection frequency was q.o.d,the duration of treatment was about 3-18 months,cytarabine (Ara-C) by slowly intravenous driped (30 mg/d).In this treatment schedule,every course of treatment sustained 10 days,and with a 2 weeks interval.During this process,patients in treatment group were treated with hydroxyl urea only when their WBC in peripheral blood exceed 20×109/L,otherwise,discontinue it.Treatment group (12 cases),on the first day of treatment with IFNα-2b and Ara-C,added indomethacin (25 mg) through oral administration,the frequency was t.i.d.During treatment in the two group,the end point of observation was completely hematology ease,at the same time,these indicators in the two groups needed to be compared,the time when WBC begin to fall,the time when WBC fall to normal range,the time when immature cells returned to normal,the time which complete hematological remission and the highest temperature of patients after IFNα-2b was subcutaneous injected.Results The time when WBC begin to fall in treatment group was (4.2±2.7) d,and the time was (5.0±2.5) d in control group (t =0.714,P ＞ 0.05).In treatment group,the time when WBC fall to normal range was(10.0±4.5) d,and the time was (12.0±4.5) d in control group (t =1.036,P ＞ 0.05).The time when immature cells returned to normal in treatment group was (14.2±4.8) d,and the time was (19.0±3.6) d in control group (t =2.609,P ＜ 0.02).The time which complete hematological remission was achieved in control group was (45.8±5.6) d,but it was (53.9±10.5) d in control group (t =2.314,· P ＜ 0.05).Meanwhile,the fever degree after IFNα-2b was subcutaneous injected obviously achieved improvement in treatment group (x2 =12.041,P ＜ 0.005).Conclusion The advantage of indomethacin to the unite treatment with IFNα-2b and LD-Ara-C on CML mainly lays in which cound alleviated the adverse reaction such as flu-like of IFN,and more,there are synergy effect in antagonist CML.

Objective To investigate the remineralization effects of the Aominqing dental desensitizer and the fluoride dentifrice on the demineralized enamels. Methods Sixty-three teeth were randomly divided into three groups after demineralization , then was remineralized for eight days by using Aominqing dental desensitizer, fluoride dentifrice (1.1 g/L), and deionized water, respectively. The thin sections of teeth were analyzed under the con-focal laser scanning microscope (CLSM). The morphology of the surface of teeth was observed under the scanning electron microscope (SEM). Results Under CLSM, the evaluation parameter area of the fluorescent lesion (A,μm2) processed by Aominqing and by fluoride was (3.19 ± 0.19) × 104, (3.61 ± 0.26) × 104 μm2, respectively. The total fluorescence (TF) was (0.61 ± 0.09) × 106, (0.89 ± 0.15) × 106, average fluorescent of the lesion(AF) was (18.98 ± 1.56), (24.65 ± 2.39), and the above parameters were all less than those in the blank control group [A=(4.89 ± 0.24) × 104 μm2,TF=(1.78 ± 0.21) × 106, AF = 36.29 ± 2.57] (P < 0.01). The evaluation parameters in the Aominqing group were less than those in the fluoride dentifrice group(P < 0.05). Under SEM, the surface of the group processed by Aominqing was the smoothest, compared to the fluoride dentifrice group and the blank control group. Conclusions Both Aominqing dental desensitizer and fluoride dentifrice (1.1 g/L) have the remineralization effects on the demineralized enamels, and the former has a stronger effect.

Aged ; Hodgkin Disease ; pathology ; therapy ; Humans ; Lymphoma, Mantle-Cell ; Male ; Stomach Neoplasms ; secondary

Abstract：Objective To analyse the level of measles antibody and serological susceptibility of the enterprise employees in Jin⁃ shan District，Shanghai in 2019，to provide references for measles prevention and control of enterprise employees. Methods A total of 1 796 employees were selected from 6 enterprises in Jinshan District by using a stratified random sampling method，of which serum samples from intravenous blood were collected and determined for IgG antibody level of measles by ELISA. Geo⁃ metric mean concentration（GMC）of measles IgG antibody，antibody positive rate and antibody protection rate were calculated and the susceptibility factors of measles in enterprise employees were analyzed by using the unconditional multivariate Logistic regression model. Results The GMC of measles antibody in 1 796 monitoring objects was 639. 71 mIU／mL，the positive rate was 87. 58% and the protection rate was 29. 06%. Multivariate Logistic regression analysis showed that the odds ratio（OR）of measles susceptibility was 1. 55（95% CI：1. 14 ~ 2. 12）in native subjects compared with non⁃resident subjects，while 2. 35 （95% CI：1. 35 ~ 4. 08）in subjects aged 40 ~ 49 years compared with those aged ≥ 50 years. Conclusion In 2019，the measles antibody level of enterprise employees in Jinshan District of Shanghai was low，and people aged 40 ~ 49，especially the nativeadults，wereathighriskofsusceptibilitytomeasles. Supplementaryimmunizationwithmeaslesvaccineshouldbecarried out for high⁃risk enterprise employees to reduce the risk of measles infection and control the epidemic situation of measles.