1.Improvement and Extension of the Function of Hospital Pharmaceutical Services
China Pharmacy 2005;0(16):-
OBJECTIVE:To explore the approach for the improvement and extension of the function of pharmaceutical services in modern hospitals.METHODS:The development of our pharmacy department in recent years was analyzed,aimed at the actual demands of hospitals and patients and in view of the advantages of our department,the new entry point for the pharmaceutical services was explored.RESULTS & CONCLUSIONS:By improving the workflow of prescription dispensing,the time for patients to have their prescriptions filled can be greatly shortened and is left for pharmacists to collect drug-related information based on the demands of both patients and clinic and provide professional guidance so as to increase the weight of patients in the treatment course,which is in the interest of medication safety,patients and hospital.
2.Quality of Life Evaluation and Influencing Factors in Patients with Stroke
Shu-Feng DAI ; Yu-Chen WU ; Dao-Jun HONG ;
International Journal of Cerebrovascular Diseases 2006;0(12):-
The evaluation of quality of life after stroke primarily includes body,psychology, society,and the ability of activities of daily living,and they can be mainly obtained from self rating quality of life by the patients,The commonly used evaluation methods include six generic measurement scales and four updated Stroke Specific Quality of Life Scales.The latter includes the Stroke Adapted Sickness Impact Profile,the Stroke Impact Scale,Stroke Specific Quality of Life Scales,and Stroke and Aphasia Quality of Life Scale.This article reviews the generic meas- urement scales,Stroke Specific Quality of Life Scales and the various factors that influencing quality of life after stroke.
3.The alkaline phosphatase analysis of 40 children with cerebral palsy
Jian-jun LIU ; Shu-rong JI ; Shu-fen JIA ; Yingyuan HU ; Weihong WU ; Yanchun LI
Chinese Journal of Rehabilitation Theory and Practice 2002;8(3):183-183
ObjectiveTo explore the relations between the alkaline phosphatase(ALP) and the type of cerebral palsy. MethodsThe ALP level of 40 children with different types of cerebral palsy were examined with the Olympus AU-600 apparatus. ResultsThe ALP value of the children with spastic cerebral palsy is normal. The ALP value of the children with athetoid cerebral palsy is higher than spastic cerebral palsy(P<0.001).Conclusions The ALP value can help us to identify the type of cerebral palsy.
4.Preparation and cytocompatibility of beta-calcium phosphate scaffolds with tanshinol coating
Tao WU ; Yingchao LIU ; Kaihui NAN ; Tao SHU ; Jinhui WU ; Zhiqin LU ; Jianzhou XING ; Jun LV
Chinese Journal of Tissue Engineering Research 2017;21(14):2247-2253
BACKGROUND: Revascularization is a challenge for the tissue-engineered bone carrying cells after implanted into human body. Previous studies have found that tanshinol can improve the functions of endothelial progenitor cells and exert vascular protective effects.OBJECTIVE: To prepare the β-calcium phosphate (β-TCP) scaffold with tanshinol coating, and to observe its cytocompatibility.METHODS: The β-TCP scaffolds coated with 10-7, 10-6 and 10-5 mol of tanshinol were constructed by negative pressure absorption method. The distribution of tanshinol coating on the scaffold was observed using scanning electron microscopy,and the inner ingredients were analyzed by infrared spectrum. Human endothelial progenitor cells (hEPCs) were cultured in the extracts of β-TCP and β-TCP scaffolds with 10-7, 10-6, 10-5 mol of tanshinol coatings, respectively. The cell proliferation was detected at 2, 4, 6, 8 and 10 days of culture; the levels of nitric oxide and vascular endothelial growth factor in the supernatants were detected at 1, 7 and 14 days of culture; the lumen formation on the matrigel was observed after 14-day culture. hEPCs were respectively seeded onto the β-TCP and β-TCP scaffolds with different dosages of tanshinol coating,and then the cell growth was observed under scanning electron microscope at 7 days.RESULTS AND CONCLUSION: The tanshinol coating evenly distributed on the inner surface of the pores, and its crystalline structure became dense with dosage increasing. Infrared spectrum analysis revealed no changes in the characteristic absorption peak of tanshinol and TCP in the scaffold. The β-TCP scaffolds with tanshinol coating could promote the proliferation of hEPCs, especially the scaffolds with 10-6 and 10-5 mol tanshinol coating. Compared with the β-TCP scaffold, the scaffolds with 10-6 and 10-5 mol tanshinol coating significantly upregulated the nitric oxide level at 14 days of culture, and significantly increased the level of vascular endothelial growth factor at 7 and 14 days of culture (P <0.05). Although it could be found in all β-TCP scaffolds with tanshinol coating, the lumen formation was the maturest in the scaffold with 10-5 mol tanshinol coating. These results suggest the β-TCP scaffolds with tanshinol coating can promote the proliferation and endothelial differentiation of hEPCs, and hold a good cytocompatibility.
5.Construction of Recombinant Adenovirus Expression Vector of Human Sema4C Gene and Its Expression in Mouse Myoblasts Cell Line C2C12
Hai-Tao WU ; Shu-Hong LIU ; Yan WU ; Jun-Die FAN ; Wen-Hong FAN ; Ming FAN ;
China Biotechnology 2006;0(04):-
To generate recombinant adenovirus expression vector of human Sema4C gene and observe its expression in mouse myoblasts cell line C2C12 for ensuring easy access to investigate the role of Sema4C gene during myogenesis. The recombinant plasmid was packaged and amplified after being transfected in HEK293 cells through Lipofectamine. After infecting C2C12 myoblasts with recombinant adenovirus vector, the adenoviral infection efficiency was determined by confocal microscope which showed that the expression of green fluorescence could be detected at 12h and then reached peak at 24h after recombinant adenovirus infection. The infection efficiency was almost 100% confirmed by FACS examination. Detection of WB indicated that the expression of Sema4C in C2C12 of recombinant adenoviral infection group was significantly higher than that of the control group (P
6.Chemical constituents from Euphorbia lunulata.
Ming ZHAO ; Shuang WU ; Jun LI ; Wan-Xia TANG ; Jin-Lan WANG ; Shu-Jun ZHANG
China Journal of Chinese Materia Medica 2014;39(12):2289-2294
The chemical constituents from Euphorbia lunulata was investigated in this paper. Fourteen compounds were isolated and purified by column chromatographies on silica gel and preparative HPLC. Their structures were identified by physiochemical properties and NMR data analysis as lupeol (1), euphol (2), cassipourol(3) , 24-methylenecycloartan-3beta-ol (4), 24-hydroperoxycycloart-25-en-3beta-ol (5), 25-hydroperoxycycloart-23-en-3beta-ol (6), betulin (7), uvaol (8), (23E) -25-methoxycycloart-23-en-3beta-ol (9), (23E) -cycloart-23,25-dien-3beta-ol (10), 24-methylenecycloartan-3beta, 28-diol (11), salicinolide (12), 2alpha, 3beta, 5alpha, 9alpha, 15beta-pentaacetoxy-11,12-epoxy-7beta, 8alpha-diisobutyryloxyjatropha-6 (17) -en-14-one (13) and 3beta, 5alpha, 15beta-triacetoxy-7beta-isobutyryloxy-9alpha-nicotinoyloxyjatropha-6 (17), 11(E)-dien-14-one (14). Among them, compounds 1-11 were isolated from E. lunulata for the first time.
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
;
Euphorbia
;
chemistry
;
Magnetic Resonance Spectroscopy
;
Molecular Structure
;
Spectrometry, Mass, Electrospray Ionization
;
Stereoisomerism
7.Application of color Doppler ultrasound in examining hypothyroidism among radiation-exposed workers.
Shu-ju SUN ; Jun-wu TAN ; Bing YU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(10):772-776
OBJECTIVETo explore the application of color Doppler ultrasound in examining hypothyroidism among radiation-exposed workers.
METHODSA total of 181 radiation-exposed workers who had clinical and laboratorial diagnoses of hypothyroidism were divided into X-ray diagnosis group, interventional radiology group, and radiotherapy group. Another 62 healthy people who underwent physical examinations were used as control group. All cases underwent color Doppler ultrasound; thyroid function, sonograms, and blood flow characteristics were analyzed.
RESULTSIn the X-ray diagnosis group, interventional radiology group, and radiotherapy group, significant differences in the levels of free thyroxine and thyroid-stimulating hormone were found across workers with different lengths of service (P < 0.05). In the X-ray diagnosis group, interventional radiology group, and radiotherapy group, the percentages of individuals having uneven internal echo intensity among clinical hypothyroidism cases were higher than the percentages among subclinical hypothyroidism cases (P < 0.05). The X-ray diagnosis group, interventional radiology group, and radiotherapy group had significantly higher constituent ratios of grade II and III blood flow signals than the control group (P < 0.05). The peak systolic velocity of the superior thyroid artery (V(max)) was significantly higher in the X-ray diagnosis group, interventional radiology group, and radiotherapy group than in the control group (P < 0.05); in each group, Vmax was significantly higher in clinical hypothyroidism cases than in subclinical hypothyroidism cases (P < 0.05).
CONCLUSIONAs a simple, non-invasive, and economic examination, color Doppler ultrasound has been demonstrated as a valuable method for evaluating occupational hypothyroidism and holds promise for clinical application.
Case-Control Studies ; Health Personnel ; Humans ; Hypothyroidism ; diagnostic imaging ; Occupational Exposure ; adverse effects ; Radiology ; Thyrotropin ; blood ; Thyroxine ; blood ; Ultrasonography, Doppler, Color
8.Application of Habib 4X in hepatic resection
Zhi DU ; Yijun WANG ; Chenxuan WU ; Qiang YUAN ; Xiang JING ; Guiming SHU ; Jun WANG ; Cheng LOU
Chinese Journal of Digestive Surgery 2011;10(1):33-35
Objective To investigate the value of Habib 4X in hepatic resection. Methods The clinical outcome of 21 patients with liver disease who received liver resection at the Tianjin Third Central Hospital from November 2009 to April 2010 were retrospectively evaluated. All the operations were carried out by using Habib 4X. Results All patients received hepatectomy, including right hepatectomy in three patients, left hepatectomy in one patient, multiple segmentectomy in nine patients, single segmentectomy in seven patients and partial liver resection in one patient. All tumors were reseeted completely. The mean operation time was (50±25) minutes and the mean blood loss was(129±117)ml. No patient was transferred to ICU. Three patients were complicated with bile leakage, one with lymphatic leakage and four with pleural effusion, and they were cured by non-surgical treatment. There were no patients with postoperative hemorrhage, incision infection or hepatic failure. No mortality was observed. The mean postoperative hospital stay was(19±14)days. Conclusions Radiofrequency energy was applied along the margins of the tumor to create zones of necrosis before resection with a scalpel, offering hepatobiliary surgeons an additional method for performing liver resections with minimal blood loss, low morbidity and mortality rates. As for malignant tumors, minor or major liver resection assisted by Habib 4X is safe, and it can reduce the chance of positive incisal margin.
10.Xijiao Dihuang decoction combined with Yinqiao powder inhibits TNF-α-induced permeability increase in PMVEC via PKC-SSeCKS pathway
Ruifang REN ; Shu ZHANG ; Xiaorui LI ; Leiming YOU ; Jun WU ; Yu HAO
Chinese Journal of Pathophysiology 2017;33(5):871-876
AIM:To investigate the molecular mechanism of Xijiao Dihuang decoction combined with Yinqiao powder (XDY) in treating viral pneumonia, and the effects of XDY on TNF-α-induced permeability in pulmonary microvascular endothelial cells (PMVEC) and the role of PKC-SSeCKS pathway involved.METHODS:The electric conductivity method was used to detect transendothelial electrical resistance (TER) of primarily cultured PMVEC on Transwell chamber at different time points to determine the permeability of PMVEC.After pretreatment for 24 h, the activity of PKC, TER, and the expression of SSeCKS at mRNA and protein levels were detected.Laser scanning confocal microscopy was used to observe the location of SSeCKS and construction of F-actin in PMVEC.RESULTS:The permeability of PMVECs induced by TNF-α reached the peak at 24 h.Compared with control group, the TER in TNF-α group was decreased, and the activity of PKC was increased.Compared with TNF-α group, the activity of PKC in TNF-α with PKC inhibitor group and TNF-α with XDY group was decreased, while the TER was increased, without difference from control group.Compared with control group, the mRNA expression of SSeCKS and phospho-SSeCKS was increased in PMVEC of TNF-α group, but decreased in TNF-α with XDY group compared with TNF-α group.In control group, F-actin was mainly located around the nucleus and at cytoplasmic borders of PMVEC, forming the dense peripheral bundle, and SSeCKS was evenly scattered in the cell.In TNF-α group, the dense peripheral bundle of F-actin surrounding the cells almost disappeared, and SSeCKS was concentrated around the nucleus.Compared with TNF-α group, the distribution and the structure of F-actin and SSeCKS nearly returned to normal in TNF-α with XDY group.CONCLUSION:XDY inhibits the activation of PKC signaling pathway in PMVEC caused by TNF-α to reduce the mRNA expression of SSeCKS and the phosphorylation of SSeCKS, thus preventing the deformation of endothelial cells and reducing the permeability of PMVEC.